scholarly journals Haemathological and histological evaluation of African catfish Clarias gariepinus following acute exposure to Methanolic extract of Khaya senegalensis

2015 ◽  
Vol 2 (3) ◽  
pp. 475-482
Author(s):  
Matouke Matouke Moise ◽  
Obadiah Audu Abui
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Methanolic Extract ◽  
Clarias Gariepinus ◽  
Histological Evaluation ◽  
African Catfish ◽  
Median Lethal Concentration ◽  
Intracellular Space ◽  
Exposed Fish ◽  
Khaya Senegalensis

Haematological and histopatholoical effects of methanolic extract of K. senegalensis leaves was investigated on Clarias gariepinus over a period of 96h exposure. The median lethal concentration of the extract was 199.69mg/L. The extract caused decreased in total erythrocytes (TEC) and Packed Cell volume (PCV) respectively and increased of total leukocytes (TLC). Histopathological lesions in the liver, cytoplasmic degeneration, less intracellular space, mild necrosis, sinusoidal blood congestion and marked blood congestion in hepathocytes were recorded. However the severity but not the type of lesions was concentration-dependent. Though, the degree of tissue change (DTC) varied with the methonolic extract used. There was significant association (P<0.05) between the DTC and K. senegalensis methanolic concentration. The cumulative DTC indicated a moderate damage in the liver. The extract was considered toxic to the exposed fish and therefore deleterious on the organs of C. gariepinus.Res. Agric., Livest. Fish.2(3): 475-482, December 2015

scholarly journals Liver histopathological changes in Clarias gariepinus exposed to ethanol extract of Adenium obesum stem bark

2015 ◽  
Vol 32 (01) ◽  
pp. 022-028 ◽  
Author(s):  
S. Abalaka ◽  
M. Fatihu ◽  
N. Ibrahim ◽  
S. Ambali
Keyword(s):  
Clarias Gariepinus ◽  
Ethanol Extract ◽  
Exposure Period ◽  
Stem Bark ◽  
Aquatic Environments ◽  
Median Lethal Concentration ◽  
Exposed Fish ◽  
Range Finding ◽  
Serum Aspartate Aminotransferase ◽  
Histopathological Effects

Abstract Introduction: Histopathological effects of ethanol extract of Adenium obesum stem bark on the liver of Clariasgariepinus was investigated over a 96-h exposure period as an effective organic piscicide. Materials and Methods: An acute static toxicity bioassay was performed after a preliminary concentration range finding test was conducted. Results: Exposed fish showed adaptation behavioural changes with those of respiratory distress and abnormal nervous signs of toxicity, which was concentration-dependent. Mortality was recorded in some of the exposed fish while a median lethal concentration of 7.15 mgL-1 was established for the extract in the exposed fish. Non-significant (p>0.05) changes were recorded in serum aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase activities of the exposed ish. Moderate liver damage was observed in the exposed fish based on the degree of tissue change (DTC) grading where significant (p<0.05) differences were observed between the DTC of the exposed groups and the control. Conclusion: Ethanol extract of A. obesum stem bark was toxic to the exposed fish with evidence of hepatotoxicity and therefore, can be used to eradicate the hardy C.gariepinus from aquatic environments.

scholarly journals Determination of Median Lethal Concentration (LC50) for Endosulfan, Heptachlor and Dieldrin Pesticides to African Catfish, Clarias gariepinus and Their Impact on Its Behavioral Patterns and Histopathological Responses

Toxics ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 340
Author(s):  
Ariful Islam ◽  
Nurul Amin ◽  
Christopher L. Brown ◽  
Abdul Shukor Juraimi ◽  
Kamal Uddin ◽  
...  
Keyword(s):  
Juvenile Fish ◽  
Clarias Gariepinus ◽  
Aquatic Organism ◽  
The Body ◽  
Lethal Concentration ◽  
African Catfish ◽  
Median Lethal Concentration ◽  
Histopathological Alterations ◽  
Behavioral Abnormalities ◽  
Hepatic Portal

Pesticides such as endosulfan, heptachlor and dieldrin persist in aquatic environments as a result of their resistance to biodegradation. However, there is no adequate information about the toxicity of endosulfan, heptachlor and dieldrin to the aquatic organism, African catfish (Clarias gariepinus)—a high valued widely distributed commercially interesting species. The current experiment was performed with the aim to determine the median lethal concentration (LC50) of endosulfan, heptachlor and dieldrin to African catfish (Clarias gariepinus); their behavioral abnormalities and histopathological alterations in several vital organs. A total of 324 juvenile fish were exposed for 96 h to six concentrations of endosulfan and dieldrin at 0, 0.001, 0.002, 0.004, 0.008 and 0.016 ppm, and to heptachlor at concentrations of 0, 0.02, 0.04, 0.08, 0.16 and 0.32 ppm for dose-response tests. The study demonstrated that the species is highly susceptible to those contaminants showing a number of behavioral abnormalities and histopathological changes in gill, liver and muscle. The 96-h LC50 value of endosulfan, dieldrin and heptachlor for the African catfish was found as 0.004 (0.001−0.01) mg/L, 0.006 mg/L and 0.056 (0.006−0.144) mg/L, respectively. Abnormal behaviors such as erratic jerky swimming, frequent surfacing movement with gulping of air, secretion of mucus on the body and gills were observed in response to the increasing exposure concentrations. Histopathological alterations of liver, gill and muscle tissues were demonstrated as vacuolization in hepatocytes, congestion of red blood cells (RBCs) in hepatic portal vein; deformed secondary lamellae and disintegrated myotomes with disintegrated epidermis, respectively. These findings are important to monitor and responsibly manage pesticide use in and around C. gariepinus aquacultural areas.

scholarly journals Cytotoxicity, Redox and Immune Status in African Catfish, Clarias Gariepinus (Burchell, 1822) Exposed to Bisphenol a (BPA) and its Analogues

2021 ◽  
Author(s):  
Oluwakemi D. Samuel ◽  
Joseph Adeyemi ◽  
Olufemi S. Bamidele ◽  
Fernando Barbosa ◽  
Chris O. Adedire
Keyword(s):  
Oxidative Stress ◽  
Bisphenol A ◽  
Blood Cells ◽  
Clarias Gariepinus ◽  
White Blood Cells ◽  
Immune Dysfunction ◽  
Toxic Effects ◽  
African Catfish ◽  
Exposed Fish ◽  
Immune Parameters

Abstract The objective of the study was to determine the comparative toxicities and immune dysfunction in the African catfish, Clarias gariepinus exposed to bisphenol A (BPA) and its two analogues; bisphenol AP (BPAP) and bisphenol P (BPP). Juveniles of C. gariepinus were exposed to sublethal concentrations (70 and 140 μg/L) of BPA, BPAP and BPP for 7, 14, or 21 days after which various endpoints which are indicative of cytotoxicity, oxidative stress, haematological and innate immune parameters were determined in the liver homogenates or blood plasma. The exposure of C. gariepinus to BPA and its analogues caused significant increased activities of lactate dehydrogenase, catalase and superoxide dismutase. The exposed fish had increased levels of DNA fragmentation, lipid peroxidation, white blood cells, nitric oxide and respiratory burst while the red blood cells counts and the percentage packed cell volume decreased significantly in the exposed fish compared to control. The toxic effects elicited by the bisphenols were both concentration- and duration-dependent. Generally, BPA exerted the most toxic effects on the fish, followed by BPAP while BPP exerted the least toxic effect effects to C. gariepinus. Summarily, the findings indicated that BPA and its two analogues studied in the research are capable of causing cytotoxicity, oxidative stress and immune dysfunction in C. gariepinus.

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

2019 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Research Result ◽  
Clarias Gariepinus ◽  
Storage Period ◽  
The Body ◽  
Garlic Extract ◽  
African Catfish ◽  
Natural Preservatives ◽  
Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

PEMBEKUAN SEMEN LELE DUMBO (Clarias Gariepinus BURCHELL 1822) SEBAGAI MODEL KRIOPRESERVASI SEMEN IKAN

2010 ◽  
Vol 9 (1) ◽  
Author(s):  
Lutfi Lutfi
Keyword(s):  
Liquid Nitrogen ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Nitrogen Vapor ◽  
Dmso Concentration ◽  
Freezing Temperatures ◽  
Vapor Liquid

<p><em>Effect of four types of diluents</em><em>s</em><em> and four concentration of DMSO (5%, 10%, 15% and 20%) against the motility of African catfish sperm were evaluated after storage at freezing temperatures. </em><em>The steps in preparation the 16 treatments combination of the diluents</em><em> </em><em>are </em><em>preparation of diluents, mixing </em><em>diluents </em><em>with DMSO, packing </em><em>of semen </em><em>in</em><em>to</em><em> 0.3-ml straw, equilibration of </em><em>semen </em><em>at 4 °C for 30 minutes, freezing </em><em>of </em><em>semen in nitrogen vapor liquid </em><em>at </em><em>a height of 6.5 cm for 10 minutes</em><em>,</em><em> and subsequent</em><em>ly</em><em> storage of </em><em>semen </em><em>in liquid nitrogen (-196</em><em> <sup>0</sup></em><em>C) for further analy</em><em>sis</em><em> </em><em>of </em><em>post-thawing motility (PTM). </em><em>The result showed that t</em><em>he highest level of motility of spermatozoa </em><em>was </em><em>in </em><em>treatment </em><em>P</em><em><sub>1</sub></em><em>D</em><em><sub>15 </sub></em><em>(45.7 ± 4.3%) and the lowest </em><em>was </em><em>in </em><em>treatment </em><em>P<sub>2</sub>D<sub>20</sub> (14.5 ± 13.2%). The best diluent </em><em>in </em><em>this observation </em><em>was </em><em>diluents containing NaCl, KCl, CaCl<sub>2</sub> and NaHCO<sub>3</sub>. The best concentration </em><em>was </em><em>DMSO 15%. While the best interaction between the concentration of DMSO diluents is P<sub>1</sub>D<sub>15</sub> treatments containing NaCl, KCl, CaCl <sub>2</sub> and NaHCO<sub>3</sub> with a combination of 15% DMSO concentration. </em><em>The </em><em>conclusion </em><em>of the research is that </em><em>diluents containing NaCl, KCl, CaCl<sub>2</sub> and NaHCO<sub>3</sub> with a combination of 15% DMSO concentration</em><em> can be used in </em><em>cryopreservation of African catfish semen</em><em>.</em></p>

Vitamin E as antioxidant in female african catfish (Clarias gariepinus) exposed to chronic toxicity of atrazine

2012 ◽  
Vol 16 (2) ◽  
pp. 83-98 ◽  
Author(s):  
Shadia Kadry ◽  
Afnan Amer ◽  
Mohamed Marzouk ◽  
Magdy Hanna ◽  
Amal Azmy ◽  
...  
Keyword(s):  
Vitamin E ◽  
Chronic Toxicity ◽  
Clarias Gariepinus ◽  
African Catfish

Evaluation of body weight and color of cultured European catfish (Silurus glanis) and African catfish (Clarias gariepinus) using image analysis

2021 ◽  
Vol 93 ◽  
pp. 102147
Author(s):  
Erkan Gümüş ◽  
Adil Yılayaz ◽  
Mahir Kanyılmaz ◽  
Bahar Gümüş ◽  
Murat Balaban
Keyword(s):  
Image Analysis ◽  
Body Weight ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Silurus Glanis ◽  
European Catfish
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