scholarly journals Evaluation of the Biofilm Forming Capacities of Bacterial Isolates Recovered in Raw and Treated Effluent from Wastewater Treatment Plant of Ahmadu Bello University Zaria, Nigeria

2019 ◽  
Vol 23 (10) ◽  
pp. 1783-1786
Author(s):  
MI Ugwoke ◽  
DA Machido ◽  
MB Tijjani

Biofilm producing bacteria are associated with many recalcitrant infections and are highly resistant to antimicrobial agents, hence notoriously difficult to eradicate. This study aimed at determining the biofilm forming capacities of bacterial isolates recovered in the raw wastewater and treated effluent from Wastewater Treatment Plants of Ahmadu Bello University Zaria using Tube Method (TM) and Congo Red Agar (CRA) method; and from the results, among the isolates recovered from the raw wastewater, TM detected 62.5% isolates as positive and 37.5% as negative for biofilm production, CRA detected 37.5% isolates as positive and 62.5% as negative for biofilm production. TM also demonstrated to be more suitable in detecting biofilm producing bacterial isolates from the treated effluent were it detected 50% isolates as positive and 50% as negative. However, CRA detected only 12.5% isolates as positive and 87.5% as negative for biofilm production. We therefore, conclude that the TM is more efficient and reliable for detection of biofilm producing bacteria in the laboratory when compared to CRA method and can be recommended as one of the suitable standard screening method for the detection of biofilm producing bacteria in laboratories.Keywords: Biofilm; Bacteria; Congo red agar and Tube method

Author(s):  
Tamara Mainetti ◽  
Marilena Palmisano ◽  
Fabio Rezzonico ◽  
Blaž Stres ◽  
Susanne Kern ◽  
...  

AbstractConjugated estrogens, such as 17β-estradiol-3-sulfate (E2-3S), can be released into aquatic environments through wastewater treatment plants (WWTP). There, they are microbiologically degraded into free estrogens, which can have harmful effects on aquatic wildlife. Here, the degradation of E2-3S in environmental samples taken upstream, downstream and at the effluent of a WWTP was assessed. Sediment and biofilm samples were enriched for E2-3S-degrading microorganisms, yielding a broad diversity of bacterial isolates, including known and novel degraders of estrogens. Since E2-3S-degrading bacteria were also isolated in the sample upstream of the WWTP, the WWTP does not influence the ability of the microbial community to degrade E2-3S.


FACETS ◽  
2018 ◽  
Vol 3 (1) ◽  
pp. 128-138 ◽  
Author(s):  
Claire N. Freeman ◽  
Lena Scriver ◽  
Kara D. Neudorf ◽  
Lisbeth Truelstrup Hansen ◽  
Rob C. Jamieson ◽  
...  

Wastewater treatment plants (WWTPs) have been identified as hotspots for antimicrobial resistance genes (ARGs) and thus represent a critical point where patterns in ARG abundances can be monitored prior to their release into the environment. The aim of the current study was to measure the impact of the release of the final treated effluent (FE) on the abundance of ARGs in the receiving water of a recently upgraded WWTP in the Canadian prairies. Sample nutrient content (phosphorous and nitrogen species) was measured as a proxy for WWTP functional performance, and quantitative PCR (qPCR) was used to measure the abundance of eight ARGs, the intI1 gene associated with class I integrons, and the 16S rRNA gene. The genes ermB, sul1, intI1, blaCTX-M, qnrS, and tetO all had higher abundances downstream of the WWTP, consistent with the genes with highest abundance in the FE. These findings are consistent with the increasing evidence suggesting that human activity affects the abundances of ARGs in the environment. Although the degree of risk associated with releasing ARGs into the environment is still unclear, understanding the environmental dimension of this threat will help develop informed management policies to reduce the spread of antibiotic resistance and protect public health.


1994 ◽  
Vol 30 (4) ◽  
pp. 25-34
Author(s):  
M. Ettala ◽  
E. Rossi

Seven operational mishaps were specified on the basis of a questionnaire on wastewater treatment plants, some of them large. In this study a process was developed for screening the chemical spill risks to municipal biological sewage treatment plant. Data on wastewater treatment processes, potential spill sources and chemical properties were combined to determine the threshold chemical quantities which may inhibit the removal of carbonaceous material, prevent nitrification and methanogenesis, cause sludge contamination or lead to the aeration capacity being exceeded. Two sewage treatment plants and eleven industrial sites were chosen for field studies. The influence of spill duration and maintenance activities on threshold quantities are discussed. A field survey lasting 1-2 hours at each site was long enough for the most relevant data to be obtained when the screening method developed was applied. Several chemical spill risks to the plants studied were specified. In addition, cases were identified in which failure of the pretreatment facilities for industrial wastewaters could cause severe mishaps at a biological wastewater treatment plant.


2020 ◽  
Vol 8 (1) ◽  
pp. 63
Author(s):  
Ni Nyoman Nepi Marleni ◽  
Ristie Ermawati ◽  
Nitis Aruming Firdaus

<span>The water demands for agricultural purposes are increasing along with the population growth. Unfortunately, the water source is not sufficiently available nowadays. Furthermore, the quality of available water is more deteriorated due to the contamination from many sources such as households, industrial areas and other activities. Water board Scheldestromen handles 16 wastewater treatment plants located in Province of Zeeland in The Netherlands. The total production of treated effluent water from 16 wastewater treatment plants is 50 million annually. Quality of the effluent is relatively good, however, some potential risks related to the number of pathogenic microorganism and some trace metals are still there. The treated effluent is mostly discharged to river near to the treatment plant. The high amount of effluent discharge would potentially to be re-used for fulfill the demand for agricultural water. Therefore, this study aims to evaluate the feasibility of treated effluent from one of Walcheren wastewater treatment plants located in Ritthem, Province of Zeeland.</span><span>This study uses the method of Multi Criteria Analysis (MCA) for looking the best way to re-use the effluent for agricultural purposes. The MCA questionnaire was developed based on the thorough literature review of effluent reuse. There are 6 criteria that need to be weighted and 6 scenario alternatives that need to be scored for the MCA questionnaire. The result show that the effluent water contained pathogenic bacteria (E.Coli) and some trace metals (Ni and Cu) more than suggested by FAO standard. Hence the most preferred technology is combination between closed conduits and UV system, with weighted score 0.78 while others had less weighted score compared to the selected one</span>


2014 ◽  
Vol 9 (3) ◽  
pp. 293-306 ◽  
Author(s):  
Adel A. S. Al-Gheethi ◽  
M. O. Abdul-Monem ◽  
A. H. S. AL-Zubeiry ◽  
A. N. Efaq ◽  
A. M. Shamar ◽  
...  

The aim of this study was to evaluate the efficiency of wastewater treatment plants (WWTPs) in Yemen for reduction of faecal indicators and pathogenic bacteria in the secondary effluents and sludge. Hundred sixty bacterial isolates were obtained from 27 secondary effluents and sludge samples generated from Ibb wastewater treatment plant (IWWTP), Taiz wastewater treatment plant (TWWTP), Aden wastewater treatment plant (AWWTP1 and 2) and Sana'a wastewater treatment plant (SWWTP) in Republic of Yemen. Isolation of the bacteria was carried out by the direct plate method on the several selective media. The concentrations of faecal coliforms (FCs) were more than that recommended by World Health Organisation guidelines in all secondary effluents samples expect for those collected from TWWTP. FCs in the sludge from IWWTP and SWWTP were more than the standards limits recommended by United State Environmental Protection Agency (U. S. EPA) Class B, while sludge from AWWTP and TWWTP meet U. S. EPA standards limits Class A and class B, respectively. Among 160 bacterial isolates, E. coli was the most common (detected in 88.88% of the samples), followed by Streptococcus faecalis (70.37%), Klebsiella pneumonia (66.67%), Enterobacter aerogenes (59.23%), Salmonella typhi (33.33%), S. typhimurium and Shigella sonni (25.93% for each) and Yersinia pestis (22.22%). The sludge samples collected from IWWTP and TWWTP and stored for 24 weeks at room temperature (25 ± 2 °C) met the standards limits recommended by U.S. EPA, Class A.


1992 ◽  
Vol 26 (9-11) ◽  
pp. 2417-2420 ◽  
Author(s):  
A. B. Pincince

In the next few years, the need to decrease emissions of odors and volatile organic compounds will become increasingly important in the design and operation of wastewater treatment plants. The primary methods for decreasing emissions are adding precipitating chemicals to raw wastewater, using velocity-controlled or vortex-type gritchambers, eliminating or decreasing free falls, covering tanks andchannels, decreasing stripping in aeration tanks, minimizing chlorine use, operating treatment plants properly, and collecting and treating vented air. The methods cannot all be used simultaneously at a single treatment plant, and some of them might prove to be too costly, except in special circumstances. Nevertheless, they are worth considering.


2013 ◽  
Vol 68 (8) ◽  
pp. 1763-1769 ◽  
Author(s):  
Lili Ma ◽  
Guannan Mao ◽  
Jie Liu ◽  
Hui Yu ◽  
Guanghai Gao ◽  
...  

As microbiological parameters are important in monitoring the correct operation of wastewater treatment plants and controlling the microbiological quality of wastewater, the abundances of total bacteria (including intact and damaged bacterial cells) and total viruses in wastewater were investigated using a combination of ultrasonication and flow cytometry. The comparisons between flow cytometry (FCM) and other cultivation-independent methods (adenosine tri-phosphate (ATP) analysis for bacteria enumeration and epifluorescence microscopy (EFM) for virus enumeration) gave very similar patterns of microbial abundance changes, suggesting that FCM is suitable for targeting and obtaining reliable counts for bacteria and viruses in wastewater samples. The main experimental results obtained were: (1) effective removal of total bacteria in wastewater, with a decrease from an average concentration of 1.74 × 108counts ml−1 in raw wastewater to 3.91 × 106counts ml−1 in the effluent, (2) compared to influent raw wastewater, the average concentration of total viruses in the treated effluent (3.94 × 108counts ml−1) exhibited no obvious changes, (3) the applied FCM approach is a rapid, easy, and convenient tool for understanding the microbial dynamics and monitoring microbiological quality in wastewater treatment processes.


2020 ◽  
Vol 15 (2) ◽  
pp. 142-151
Author(s):  
Peter Lukac ◽  
Lubos Jurik

Abstract:Phosphorus is a major substance that is needed especially for agricultural production or for the industry. At the same time it is an important component of wastewater. At present, the waste management priority is recycling and this requirement is also transferred to wastewater treatment plants. Substances in wastewater can be recovered and utilized. In Europe (in Germany and Austria already legally binding), access to phosphorus-containing sewage treatment is changing. This paper dealt with the issue of phosphorus on the sewage treatment plant in Nitra. There are several industrial areas in Nitra where record major producers in phosphorus production in sewage. The new wastewater treatment plant is built as a mechanicalbiological wastewater treatment plant with simultaneous nitrification and denitrification, sludge regeneration, an anaerobic zone for biological phosphorus removal at the beginning of the process and chemical phosphorus precipitation. The sludge management is anaerobic sludge stabilization with heating and mechanical dewatering of stabilized sludge and gas management. The aim of the work was to document the phosphorus balance in all parts of the wastewater treatment plant - from the inflow of raw water to the outflow of purified water and the production of excess sludge. Balancing quantities in the wastewater treatment plant treatment processes provide information where efficient phosphorus recovery could be possible. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. There are also two outflows - drainage of cleaned water to the recipient - the river Nitra - 9.9 kg Ptot/day and Ptot content in sewage sludge - about 120.3 kg Ptot/day - total 130.2 kg Ptot/day.


2008 ◽  
Vol 3 (1) ◽  
Author(s):  
Luchien Luning ◽  
Paul Roeleveld ◽  
Victor W.M. Claessen

In recent years new technologies have been developed to improve the biological degradation of sewage sludge by anaerobic digestion. The paper describes the results of a demonstration of ultrasonic disintegration on the Dutch Wastewater Treatment Plant (WWTP) Land van Cuijk. The effect on the degradation of organic matter is presented, together with the effect on the dewatering characteristics. Recommendations are presented for establishing research conditions in which the effect of sludge disintegration can be determined in a more direct way that is less sensitive to changing conditions in the operation of the WWTP. These recommendations have been implemented in the ongoing research in the Netherlands supported by the National Institute for wastewater research (STOWA).


1993 ◽  
Vol 28 (10) ◽  
pp. 1-8 ◽  
Author(s):  
A. Gaber ◽  
M. Antill ◽  
W. Kimball ◽  
R. Abdel Wahab

The implementation of urban village wastewater treatment plants in developing countries has historically been primarily a function of appropriate technology choice and deciding which of the many needy communities should receive the available funding and priority attention. Usually this process is driven by an outside funding agency who views the planning, design, and construction steps as relatively insignificant milestones in the overall effort required to quickly better a community's sanitary drainage problems. With the exception of very small scale type sanitation projects which have relatively simple replication steps, the development emphasis tends to be on the final treatment plant product with little or no attention specifically focused on community participation and institutionalizing national and local policies and procedures needed for future locally sponsored facilities replication. In contrast to this, the Government of Egypt (GOE) enacted a fresh approach through a Local Development Program with the United States AID program. An overview is presented of the guiding principals of the program which produced the first 24 working wastewater systems including gravity sewers, sewage pumping stations and wastewater treatment plants which were designed and constructed by local entities in Egypt. The wastewater projects cover five different treatment technologies implemented in both delta and desert regions.


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