scholarly journals Invivo Antimalarial Activity of Dodonaea Angustifolia Seed Extracts Against Plasmodium Berghei in Mice Model

2012 ◽  
Vol 4 (1) ◽  
pp. 47 ◽  
Author(s):  
B Mengiste ◽  
E Makonnen ◽  
K Urga
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Mice Model ◽  
Dodonaea Angustifolia

scholarly journals Antimalarial Activity of Meriandra dianthera Leaf Extracts in Plasmodium berghei-Infected Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Kalay Hagazy ◽  
Gereziher G. Sibhat ◽  
Aman Karim ◽  
Gebretsadkan H. Tekulu ◽  
Gomathi Periasamy ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Mice Model ◽  
Oral Toxicity ◽  
Leaf Extracts ◽  
Traditional Use ◽  
Crude Leaf Extract

Objective. To evaluate the antimalarial effect of aqueous methanolic extract and solvent fractions of Meriandra dianthera leaves against Plasmodium berghei in mice model. Method. M. dianthera leaves were extracted with 80% methanol and dried. The dried crude extract was then defatted and further fractionated with chloroform, ethyl acetate, and butanol. Acute oral toxicity test was performed as per the Organization for Economic Cooperation and Development guideline 425. Peter’s 4-day suppressive test was used to determine the in vivo antimalarial activity of the extract and fractions. Result. The crude leaf extract of Meriandra dianthera showed parasite inhibition of 42.28% and 45.52% at doses of 400 and 600 mg/kg, respectively, as compared to the negative control. Moreover, the mice which received chloroform and aqueous fractions at the dose of 400 mg/kg/day showed significant (P<0.001) chemosuppression compared to the negative control. Both the extract and fractions were able to prevent P. berghei induced body weight loss and body temperature reduction and also increased the survival time of the mice as compared to the negative control. The aqueous methanolic leaf extract of M. dianthera showed no gross signs of toxicity or mortality in mice until a single oral dose of 2000 mg/kg. Conclusion. The extracts of M. dianthera leaves showed promising antimalarial activity, with no sign of toxicity and therefore may support its traditional use for the treatment of malaria.

Antimalarial Activity of the Methanol Aerial Extract of Alysicarpus glumaceus (Vahl) DC. in Plasmodium berghei Infected Mice

2019 ◽  
Vol 3 (3) ◽  
pp. 80-84
Author(s):  
Amina Olorukooba ◽  
◽  
Ferhat Khan ◽  
Charles Madu ◽  
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity

scholarly journals Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 532
Author(s):  
Hae-Soo Yun ◽  
Sylvatrie-Danne Dinzouna-Boutamba ◽  
Sanghyun Lee ◽  
Zin Moon ◽  
Dongmi Kwak ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Hematological Parameters ◽  
Significant Inhibition ◽  
Ic50 Values ◽  
The Republic

In traditional Chinese medicine, Ranunculus japonicus has been used to treat various diseases, including malaria, and the young stem of R. japonicus is consumed as a food in the Republic of Korea. However, experimental evidence of the antimalarial effect of R. japonicus has not been evaluated. Therefore, the antimalarial activity of the extract of the young stem of R. japonicus was evaluated in vitro using both chloroquine-sensitive (3D7) and chloroquine-resistant (Dd2) strains; in vivo activity was evaluated in Plasmodium berghei-infected mice via oral administration followed by a four-day suppressive test focused on biochemical and hematological parameters. Exposure to extracts of R. japonicus resulted in significant inhibition of both chloroquine-sensitive (3D7) and resistant (Dd2) strains of P. falciparum, with IC50 values of 6.29 ± 2.78 and 5.36 ± 4.93 μg/mL, respectively. Administration of R. japonicus also resulted in potent antimalarial activity against P. berghei in infected mice with no associated toxicity; treatment also resulted in improved hepatic, renal, and hematologic parameters. These results demonstrate the antimalarial effects of R. japonicus both in vitro and in vivo with no apparent toxicity.

scholarly journals Sequestration of erythrocytes infected with Plasmodium berghei ANKA in BALB/c mice treated with goat bile

2020 ◽  
Vol 4 (2) ◽  
pp. 179
Author(s):  
Kartika Arum Wardani ◽  
Kholida Nur Aini ◽  
Heny Arwati ◽  
Willy Sandhika
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Control Group ◽  
Dependent Manner ◽  
Plasmodium Berghei Anka ◽  
Concentration Dependent Manner ◽  
Control Group Design ◽  
Bile Concentration

Abstract Sequestration of Plasmodium berghei ANKA-infected erythrocytes occurs in BALB/c mice as characteristic of  Plasmodium falciparum infection in humans. Animals’ bile has been widely used for centuries in Traditional Chinese Medicine. Goat bile has been used in healing infectious and non-infectious diseases; however, no report on the use of goat bile against malaria infection and sequestration. The purpose of this study was to analyze the correlation between parasitemia and sequestration in the liver of P.berghei ANKA-infected BALB/c mice treated with goat bile. This research was an in vivo experimental study using the post-test control group design. The male BALB/c mice aged ± 6 weeks, body weight 20-25 g were used. The mice were divided into five groups where Group 1-3 were mice treated with goat bile 25%, 50%, and 100%, respectively. Group 4-5 were negative (sterile water) and positive controls (DHP). Parasitemia was observed daily from each mouse and the number of sequestered infected erythrocytes on the endothelium of sinusoids. The data were analyzed using t independent test. Antimalarial activity of goat bile was shown by the lower parasitemia in goat bile-treated mice compared with the negative control. The average number of sequestration was goat bile concentration-dependent manner. The higher the concentration, the lower the number of sequestration. Sequestration was correlated with parasitemia (p=0,0001). Sequestration of P.berghei ANKA-infected erythrocytes correlated with parasitemia, and was goat bile concentration-dependent manner. Keywords: Malaria, parasitemia, sequestration, goat bileCorrespondence: [email protected]

scholarly journals Experimental and Mathematical Model for the Antimalarial Activity of Methanolic Root Extract of Azadirachta indica (Dongoyaro) in Mice Infected with Plasmodium berghei NK65

2020 ◽  
pp. 68-82
Author(s):  
Adeniyi Michael Olaniyi ◽  
Momoh Johnson Oshiobugie ◽  
Aderele Oluwaseun Raphael
Keyword(s):  
Mathematical Model ◽  
Azadirachta Indica ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Equilibrium Points ◽  
Standard Procedure ◽  
Root Extract ◽  
Globally Asymptotically Stable ◽  
The Stability ◽  
Group B

The study determines the experimental and mathematical model for the anti-plasmodial activity of methanolic root extract of Azadirachta indica in Swiss mice infected with Plasmodium berghei NK65. Phytochemical analyses, antimalarial activity of the methanolic root extract of A. indica was determined in mice infected with Plasmodium berghei NK65 using standard procedure. Liver biomarker enzymes were also determined. The model P. berghei induced free and P. berghei infected equilibrium were determined. The stability of the model equilibrium points was rigorously analyzed. The phytochemicals present in the extract include: alkaloid, flavonoid, saponin and phenolic compounds etc. The experimental study consists of five groups of five mice each per group. Group A, B, C, D and E were healthy, infected without treatment, infected mice treated with fansidar (10 mg/kg), chloroquine (10 mg/kg) and 250 mg/kg body weight of A. indica methanolic root extract respectively. The extract showed anti-plasmodial activity of 73.96%. The result was significant when compared with group B mice, though it was lower than that exhibited by fansidar (88.91%) and chloroquine (92.18%) for suppressive test. There were significant decrease (P<0.05) in plasma AST and ALT levels in the treated infected mice compared to the infected untreated mice. The results of the model showed that the P.berghei induced free equilibrium is locally and globally asymptotically stable at threshold parameter,  less than unity and unstable when  is greater than unity. Numerical simulations were carried out to validate the analytic results which are in agreement with the experimental analysis of this work.

scholarly journals Antimalaria and Hematological Properties of Ethanol Leaf Extract of Pennisetum purpureum on Plasmodium berghei Infected Mice

2019 ◽  
pp. 1-8
Author(s):  
E. N. Ekene ◽  
O. M. Odigie
Keyword(s):  
Body Weight ◽  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Antimalarial Activity ◽  
Pennisetum Purpureum ◽  
High Dose ◽  
Control Groups ◽  
Parasite Count ◽  
Genus Plasmodium ◽  
Group I

Through bite from a female Anopheles mosquito, Malaria is transmitted by infection with single-celled parasites of the genus Plasmodium. Studies have shown it to be characterized by periodic bouts of severe chills, accompanied with high fever. It has been suggested that Pennisetum purpureum possess antiplasmodial effects, however, no scientific record(s) yet exist(s) to validate this claim. This study was therefore undertaken to determine the anti-malaria and haematological properties of ethanol leaf extract of P. purpureum in Plasmodium berghei -infected mice. Thirty-Five (35) albino mice (20g) were procured, acclimatized (for two weeks) and assigned to five groups of 7 mice each. With group I receiving standard rat feed ad-libitum (control), Groups II through V were respectively infected with Plasmodium berghei (malaria infected, untreated), Plasmodium berghei infected + treated with 5mg/kg body weight of Artesunate (malaria infected, Artesunate treated), infected with Plasmodium berghei + treated with 200mg/kg body weight of Pennisetum  purpureum (malaria infected, low dose extract treated), and infected with Plasmodium berghei + treated with 400mg/kg body weight of Pennisetum  purpureum (malaria infected, high dose extract treated). After 21 days of administration, mice were sacrificed, blood samples collected, centrifuged for 10 minutes at 300g, and resulting supernatant biochemically analysed for hematologic changes. Result showed a significant increase in initial parasite count across groups except control. Administration of Artesunate also caused a significant (p < .05) reduction in parasite counts upon comparison with control. More so, administration of low and high dose extract caused a significant (p < .05) reduction in parasite count following comparison with control. Administration of 200mg/kg caused the highest parasitemia suppression than high dose. We recommend for further evaluation of the plant in other to identify active ingredients responsible for the observed antimalarial activity.

scholarly journals In-vivo antimalarial activity of aqueous leaf and bark extracts of Trema orientalis against Plasmodium berghei in mice

2016 ◽  
Vol 41 (2) ◽  
pp. 398-404 ◽  
Author(s):  
Oluwatoyosi Eniola Oyebola ◽  
Olajumoke Abimbola Morenikeji ◽  
Isaiah Oluwafemi Ademola
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Trema Orientalis

scholarly journals Study of the Antimalarial Activity of the Leaf Extracts and Fractions of Persea americana and Dacryodes edulis and Their HPLC Analysis

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Philip F. Uzor ◽  
Chukwuebuka K. Onyishi ◽  
Adaeze P. Omaliko ◽  
Somtochukwu A. Nworgu ◽  
Onyemaechi H. Ugwu ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Hplc Analysis ◽  
Antimalarial Activity ◽  
Persea Americana ◽  
Leaf Extracts ◽  
Lead Compounds ◽  
Phytochemical Composition ◽  
Potential Sources ◽  
Water Fractions ◽  
Dacryodes Edulis

In the present study, the antimalarial activity of the extracts and fractions of the leaves of Persea americana and Dacryodes edulis as well as their phytochemical compositions were examined. Each of the extracts of the plants was successively fractionated to obtain hexane, ethyl acetate, methanol, and water fractions. The extracts and fractions were tested against Plasmodium berghei in both curative and suppressive antimalarial mouse models. Their major phytochemical composition was studied by the standard chemical tests and HPLC analysis. The extracts and fractions of P. americana and D. edulis demonstrated significant ( p < 0.05 ) maximal plasmodial inhibition as 52.16 ± 2.77% and 57.10 ± 1.98%, respectively, and chemosuppression of parasitemia as 64.01 ± 0.08% and 71.99 ± 0.06%, respectively. The major secondary metabolites identified in the plants include alkaloids, flavonoids, and saponins. It was concluded that P. americana and D. edulis possess promising antimalarial activity and they are potential sources of new lead compounds against malaria.

scholarly journals Antimalarial activity of hydromethanolic extract and its solvent fractions of Vernonia amygdalina leaves in mice infected with Plasmodium berghei

2019 ◽  
Vol 7 ◽  
pp. 205031211984976 ◽  
Author(s):  
Temesgen Bihonegn ◽  
Mirutse Giday ◽  
Getnet Yimer ◽  
Abebe Animut ◽  
Mekonnen Sisay
Keyword(s):  
Weight Loss ◽  
Survival Time ◽  
Rectal Temperature ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Methanol Extract ◽  
Antimalarial Activity ◽  
Vernonia Amygdalina ◽  
Oral Toxicity

Background: Vernonia amygdalina Del. (Asteraceae) is reported to be traditionally used for the treatment of malaria. Based on folkloric repute of this plant in Ethiopian traditional medicine and crude extract-based ethnopharmacological studies conducted in few countries, this study was undertaken to evaluate the in vivo antimalarial activity of 80% methanol extract and its solvent fractions of the leaves of V. amygdalina in mice infected with Plasmodium berghei. Methods: A 4-day suppressive test was conducted on mice infected with P. berghei to find out antimalarial effect of chloroform, butanol and aqueous fractions obtained from the 80% methanol crude extract. In all the activity tests, mice were randomly assigned in five groups (three tests and two controls) of six animals in each and received respective treatments. Data were analyzed using one way analysis of variance followed by Tukey’s post hoc test for multiple comparisons. Results: Acute oral toxicity test showed that all solvent fractions of the leaves of V. amygdalina revealed neither mortality nor overt signs of toxicity up to 2000 mg/kg. This study indicated that the percentage parasitemia suppression of 80% methanol extract was 32.47% (±2.65), 35.40% (±3.14) and 37.67% (±2.50) at 200, 400 and 600 mg/kg, respectively. All doses of the 80% methanol extract of V. amygdalina prolonged survival time and prevented weight loss and packed cell volume reduction in infected mice. All doses of chloroform and butanol fractions significantly suppressed parasitemia (p < 0.05), increased survival time (p < 0.05) compared to negative control and exhibited a significant reduction in rectal temperature (p < 0.05). All solvent fractions significantly prevented weight loss (p < 0.05) at all tested doses. The 80% methanol extract and chloroform and butanol fractions significantly (p < 0.05) prevented further reduction in rectal temperature of P. berghei-infected mice at all doses. Conclusion: The results of this study indicated that 80% methanol extract and solvent fractions of the leaves of V. amygdalina demonstrated promising antimalarial activity. The study corroborated the folklore use of this plant for the treatment of malaria in ethnomedicine in Ethiopia.

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