Occurrence and Survival of Campylobacter jejuni in Milk and Turkey1

1982 ◽  
Vol 45 (13) ◽  
pp. 1218-1220 ◽  
Author(s):  
C. JANE WYATT ◽  
E. M. TIMM
Keyword(s):  
Campylobacter Jejuni ◽  
Agar Medium ◽  
Raw Milk ◽  
Brucella Broth ◽  
Enrichment Procedure ◽  
Whole Milk ◽  
Rectal Swabs

An enrichment procedure was used to determine the presence of Campylobacter jejuni in milk and ground turkey. This procedure consisted of subculturing a sample in antibiotic-supplemented brucella broth incubated at 37°C for 24 h, transferred to fresh broth, incubated microaerophilically at 42°C for 8 h and plated on agar medium selective for C. jejuni for detection. C. jejuni was suspected in 9 of 50 samples of raw milk, but was not confirmed. The organism was not recovered from rectal swabs of cows. Storage of whole milk and ground turkey inoculated with C. jejuni at 4, 37 and 42°C resulted in decreases in C. jejuni counts in milk at 4 and 42°C; and increases in counts in ground turkey at 37 and 42°C. No survivors were detected when suspensions of the organism were exposed to 10 ppm chlorine for 30 s or three common commercial sanitizers used according to manufacturers' specifications.

Campylobacter jejuni in Cattle and Raw Milk in The Netherlands

1982 ◽  
Vol 45 (13) ◽  
pp. 1212-1213 ◽  
Author(s):  
J. OOSTEROM ◽  
G. B. ENGELS ◽  
R. PETERS ◽  
R. POT
Keyword(s):  
The Netherlands ◽  
Campylobacter Jejuni ◽  
Dairy Farms ◽  
Raw Milk ◽  
Direct Plating ◽  
Enrichment Procedure

A survey was done on the occurrence of Campylobacter jejuni in slaughtered cattle and raw milk from dairy farms in The Netherlands, In the first part of the survey, in which direct plating techniques were used, no C. jejuni was detected in any of 200 samples of caecal contents of cattle or in 200 samples of raw milk. A second series of investigations was done using a new enrichment procedure. This time C. jejuni was isolated from 11 of 200 caecal contents, but from none of 200 samples of milk. Further experiments showed that Campylobacter can survive in milk at 4°C for weeks, whether the milk was shaken with air (as occurs during the milking process) or not. Our investigations indicate that C. jejuni was not excreted with the milk. It can be concluded that cattle in The Netherlands do not play an important role in the epidemiology of C. jejuni.

scholarly journals Gastrointestinal infections caused by consumption of raw drinking milk in England & Wales, 1992–2017

2019 ◽  
Vol 147 ◽  
Author(s):  
N. Adams ◽  
L. Byrne ◽  
J. Edge ◽  
A. Hoban ◽  
C. Jenkins ◽  
...  
Keyword(s):  
Public Health ◽  
Infectious Disease ◽  
Campylobacter Jejuni ◽  
Raw Milk ◽  
National Surveillance ◽  
Gastrointestinal Infections ◽  
Food Standards ◽  
The Uk ◽  
Drinking Milk ◽  
Intestinal Infectious Disease

Abstract Systematic, national surveillance of outbreaks of intestinal infectious disease has been undertaken by Public Health England (PHE) since 1992. Between 1992 and 2002, there were 19 outbreaks linked to raw drinking milk (RDM) or products made using raw milk, involving 229 people; 36 of these were hospitalised. There followed an eleven-year period (2003–2013) where no outbreaks linked to RDM were reported. However, since 2014 seven outbreaks of Escherichia coli O157:H7 (n = 3) or Campylobacter jejuni (n = 4) caused by contaminated RDM were investigated and reported. Between 2014 and 2017, there were 114 cases, five reported hospitalisations and one death. The data presented within this review indicated that the risk of RDM has increased since 2014. Despite the labelling requirements and recommendations that children should not consume RDM, almost a third of outbreak cases were children. In addition, there has been an increase in consumer popularity and in registered RDM producers in the UK. The Food Standards Agency (FSA) continue to provide advice on RDM to consumers and have recently made additional recommendations to enhance existing controls around registration and hygiene of RDM producers.

Assessment of different selective agar media for enumeration and isolation ofListeriafrom dairy products

1988 ◽  
Vol 55 (4) ◽  
pp. 579-583 ◽  
Author(s):  
Lucas Dominguez ◽  
José Francisco Fernández ◽  
Victor Briones ◽  
José Luis Blanco ◽  
Guillermo Suárez
Keyword(s):  
Dairy Products ◽  
Agar Medium ◽  
Raw Milk ◽  
Superior Performance ◽  
Direct Plating ◽  
Selective Agar ◽  
Natural Microflora ◽  
Agar Media ◽  
The Difference ◽  
Better Than

SummaryDifferent selective agar media were compared for the recovery and isolation of five species ofListeriafrom raw milk and cheese. The selective media examined were Beerens medium, MacBride medium and that described by Dominguezet al.(1984) with 6 mg/1 acriflavine, listeria selective agar medium (LSAM), and LSAM with 12 mg/1 acriflavine (LSAM × 2A); a non-selective yeast glucose Lemco agar was included for comparison. When the difference between listeria and the natural microflora of raw milk and cheese was 102cfu/ml, listeria could be isolated by direct plating on all media tested. When it was lower than 103–104cfu/ml, listeria were isolated by direct plating only on LSAM and LSAM × 2A. When the difference was greater than 104cfu/ml, a previous enrichment was necessary to isolate them. LSAM and LSAM × 2A media performed better than the other media tested for isolating listeria by direct plating and improved their isolation from dairy products. This superior performance was evaluated by the ability of these media to support colony formation of different species ofListeriatested, the easy recognition of these colonies from those formed by other microorganisms and by their capacity to inhibit the natural microflora of these foods.

Evaluation of the Bacteriological Health Risk of 60-Day Aged Raw Milk Cheddar Cheese

1984 ◽  
Vol 47 (7) ◽  
pp. 530-531 ◽  
Author(s):  
MICHAEL H. BRODSKY
Keyword(s):  
Staphylococcus Aureus ◽  
Alkaline Phosphatase ◽  
Health Risk ◽  
Campylobacter Jejuni ◽  
Alkaline Phosphatase Activity ◽  
Geometric Mean ◽  
Cheddar Cheese ◽  
Raw Milk ◽  
Fecal Coliforms ◽  
Salmonella Spp

One hundred twenty-seven 60-d aged Cheddar cheese samples produced by 21 provincially inspected cheese plants were analyzed by 8 regional laboratories of the Ontario Ministry of Health. Coliforms were detected in 37 (31.2%) and fecal coliforms confirmed in 22 (18.3%) samples, with geometric mean counts per g of 92.5 and 79.3, respectively. Staphylococcus aureus was found in only two products at a level of >1000 per g. Salmonella spp. and Campylobacter jejuni were not isolated from any of the samples tested. Yersinia enterocolitica was isolated from one product; however, the isolate was bile esculin-and salicin-positive, and considered a non-pathogenic biotype. The pH of these aged Cheddars ranged between 4.98 and 5.50, with a mean of 5.26. Alkaline phosphatase activity was detected in 94 (79.7%) of the 118 samples tested. These results suggest that 60-d aged raw milk Cheddar cheese produced in Ontario does not pose a significant bacteriological health risk.

Diluents and the Enumeration of Stressed Campylobacter jejuni

1985 ◽  
Vol 48 (2) ◽  
pp. 135-137 ◽  
Author(s):  
DEBRA D. ABRAM ◽  
NORMAN N. POTTER
Keyword(s):  
Campylobacter Jejuni ◽  
Phosphate Buffer ◽  
Aerobic Conditions ◽  
Brucella Broth ◽  
Peptone Water

Significant discrepancies in calculated counts of Campylobacter jejuni strains ATCC 33250 and 29428 were observed between undiluted samples and samples diluted in 0.1% peptone water when cultures had been incubated at 42°C under micro-aerobic conditions in brucella broth containing 2% NaCl. The influence of 0.1% peptone water, brucella broth and phosphate buffer as diluents for enumeration of C. jejuni stored at 6 and −18°C in brucella broth with 0.5 and 2% NaCl was studied. The three diluents were equally effective for the recovery of C. jejuni from refrigerated broth containing 0.5% NaCl. However, when the organism had been refrigerated in the broth with 2% NaCl or frozen in the broth with either level of salt, dilution with brucella broth produced significantly higher counts than dilution with 0.1% peptone water or phosphate buffer.

scholarly journals Uses of milk in sweetmeat shops and consumer preferences to milk products at Mymensingh municipality in Bangladesh

2016 ◽  
Vol 2 (2) ◽  
pp. 266-273
Author(s):  
ABM Kawsar Ahmed ◽  
Md Rezwanul Habib ◽  
Samia Afrin ◽  
Mohammad Ashiqul Islam ◽  
Md Harun Ur Rashid
Keyword(s):  
Data Analysis ◽  
Shelf Life ◽  
Distribution Pattern ◽  
Statistical Methods ◽  
Consumer Preferences ◽  
Raw Milk ◽  
Milk Products ◽  
Whole Milk ◽  
Significant Difference ◽  
Research Findings

The work has been designed to investigate the utilization of milk for consumption of fluid milk and milk products, their distribution pattern, pricing, shelf life and consumer’s preference of Mymensingh municipality in Bangladesh. The study was based on milk and milk products and data were collected from the selected sweetmeat shops by direct interview, of which 20 samples were collected from sweetmeat shops and 7 from goalas. Both tabular and statistical methods were used for collected data analysis. Shopkeepers of different sweetmeat shops received raw milk from farmers (52.6%) and goalas (47.4%) and the highest amount of whole milk was required in per unit production of rasomalai (21%) and ghee (18%) whereas the lowest amount in chomchom (9%). Milk products prices were not remained same throughout the year in this municipality due to fluctuation of raw milk availability and their price. Eid, Puja festivals and other educational activities results that may increase milk products selling especially rasogolla and kalojam. Research findings also showed non-significant difference in case of pricing, distribution pattern, shelf life and selling of milk and milk products following sweetmeats.Asian J. Med. Biol. Res. June 2016, 2(2): 266-273

Methods for Recovery of Campylobacter jejuni from Foods

1982 ◽  
Vol 45 (14) ◽  
pp. 1332-1337 ◽  
Author(s):  
NORMAN J. STERN
Keyword(s):  
Campylobacter Jejuni ◽  
Gas Flow ◽  
Raw Milk ◽  
Growth Conditions ◽  
Food Samples ◽  
Human Infection ◽  
Animal Products ◽  
Enrichment Broth ◽  
Selective Media ◽  
Sensitivity And Selectivity

The triangular relationship between Campylobacter jejuni, foods and disease in humans has been well-documented. Many studies have revealed that C. jejuni causes at least as many cases of human gastroenteritis as does Salmonella sp. Foods are an important vehicle in human infection, and raw milk is most frequently implicated. Other animal products also serve as potential sources of infection. C. jejuni has been found on the carcasses of poultry and other domestic animals throughout the world. The organism is microaerophilic and various methods for establishing appropriate growth conditions, such as the Fortner principle, atmosphere replacement and adding of supplements to encourage growth of C. jejuni, are available. Methods developed for use in clinical laboratories lack the necessary sensitivity and selectivity, and therefore have limited use in detecting small numbers of C. jejuni in foods. In one enrichment method for detecting C. jejuni in foods, washings are filtered and centrifuged, the sediment is suspended in the enrichment broth and the suspension is incubated under a constant gas flow at reduced oxygen levels. Following incubation enrichment broth is filtered and plated onto selective media. In another recently developed method, food samples are directly added to an enrichment broth with antibiotics and incubated under a microaerobic atmosphere before selective plating. Butzler's, Skirrow's and Campy-BAP selective media use several antibiotics to which C. jejuni is resistant. The plates are supplemented with horse or sheep blood, depending upon the specific formulation. The optimum temperature for growth of C. jejuni, about 42°C, may also be used for selection. It is now possible to recover 0.1 to 1 cell of C. jejuni per 10 to 25 g of food sample from among 106 to 109 indigenous bacteria. After a characteristic colony is isolated, the key criteria for presumptive identification of C. jejuni by phase-contrast microscopy are darting, corkscrew motion and a comma to spiral shape.

Occurrence of Listeria monocytogenes in Raw Milk in Nebraska

1988 ◽  
Vol 51 (11) ◽  
pp. 840-841 ◽  
Author(s):  
MICHAEL B. LIEWEN ◽  
MARK W. PLAUTZ
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Farms ◽  
Raw Milk ◽  
Storage Tanks ◽  
Biochemical Tests ◽  
Bulk Storage ◽  
Milk Samples ◽  
Listeria Species ◽  
Enrichment Procedure

Raw milk samples were obtained from bulk storage tanks of individual dairy farms in eastern Nebraska during February and July of 1986. One hundred different farms were tested during each period. One-tenth ml of each sample was plated directly onto McBride's Listeria Agar (MLA) and 30 ml was subjected to a four-week cold enrichment procedure. Suspect colonies from MLA were subjected to biochemical tests to confirm identity. Nine percent of all raw milk samples examined were determined to be positive for Listeria species after the cold enrichment procedure. Four percent contained L. monocytogenes and five percent contained L. innocua. Six percent and two percent of samples were found to contain L. monocytogenes in February and July respectively.

Fluorometric Analysis of Alkaline Phosphatase in Fluid Dairy Products

1990 ◽  
Vol 53 (7) ◽  
pp. 588-591 ◽  
Author(s):  
RICHARD M. ROCCO
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Products ◽  
Raw Milk ◽  
Test Sample ◽  
Reaction Rates ◽  
Product Formation ◽  
Test Time ◽  
Whole Milk ◽  
Repeated Analysis ◽  
Total Test Time

A new quantitative assay has been developed for measuring residual alkaline phosphatase (ALP) activity in a wide variety of dairy products including whole milk, low fat and skim milks, chocolate milk, and creams. ALP in the test sample hydrolyzes a nonfluorescent substrate, FluorophosR, to a highly fluorescent product. Product formation is monitored continuously during a short incubation period and enzyme activity is calculated from the rate of fluorescence increase. Total test time is 3 min. Reaction rates are linear up to 0.5% raw milk (equivalent to 5 μg phenol/ml/15 min) with a detection limit of 0.006% raw milk. Within and between run precision of the fluorometric method was assessed by repeated analysis of a pasteurized milk sample containing added mixed herd raw milk. The within run (N=10) mean was 190.4 mU/L, standard deviation (SD) 3.2, and a coefficient of variance (CV) of 1.7%. The procedure provides a rapid, sensitive, precise, and easy-to-use ALP assay, applicable to a wide variety of dairy products.

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