Prevalence of Salmonellae, Listeriae, and Yersiniae in the Slaughterhouse Environment and on Work Surfaces, Equipment, and Workers

1997 ◽  
Vol 60 (4) ◽  
pp. 367-371 ◽  
Author(s):  
MICHELA LUCIA SAMMARCO ◽  
GIANCARLO RIPABELLI ◽  
ADDOLORATO RUBERTO ◽  
GIORGIO IANNITTO ◽  
GUIDO MARIA GRASSO
Keyword(s):  
Listeria Monocytogenes ◽  
Yersinia Enterocolitica ◽  
Degree Of Contamination ◽  
Salmonella Spp ◽  
Hand Wash ◽  
Cold Room ◽  
Molise Region

In 1995 and 1996 a nine-month study was carried out in 11 pig abattoirs located in the Molise region (Italy) to evaluate the degree of contamination of- the slaughterhouse environment, work surfaces, equipment, and personnel by Salmonella spp., Listeria spp., and Yersinia spp. A total of 219 samples were taken over three replications including slaughtering floor and wall, hooks, work-tables, chopping blocks, knives, cleavers, dehairing devices, hands of personnel, clothing, hand-wash basins, and cold room handles, floor, wall, and hooks. Overall, six abattoirs (54.5%) had one or more positive sites, while only 14 of the 219 sites (6.4%) tested were positive for any of considered microorganisms. Salmonella spp. were isolated from 1 of 9 cleavers (11.1 %), 1 of 16 worktables (6.25%), and 1 of 18 slaughtering floors (5.6%). Yersinia enterocolitica was found on 3 slaughtering floors (16.7%) and on 2 worktables (12.5%). Yersinia kristensenii was detected on 2 slaughtering floor swabs (11.1 %). Listeria monocytogenes was isolated from 2 of 20 cold room floor swabs (13.3%) and from 1 of 14 hand-wash basins (7.1%). Other species of Listeria were detected on slaughtering wall and floor swabs and on chopping blocks. Our study indicates that slaughtering floors, cold room floors, and worktables are important sites in abattoirs that may possibly harbor pathogens like Salmonella spp., Yersinia enterocolitica, and Listeria monocytogenes, and that cleaning and sanitizing of the slaughterhouse environment and equipment need a greater emphasis.

The Incidence of Salmonella, Campylobacter, and Yersinia enterocolitica in Swine Carcasses and the Slaughterhouse Environment

1989 ◽  
Vol 52 (9) ◽  
pp. 642-645 ◽  
Author(s):  
AKIER A. MAFU ◽  
R. HIGGINS ◽  
M. NADEAU ◽  
G. COUSINEAU
Keyword(s):  
Yersinia Enterocolitica ◽  
Degree Of Contamination ◽  
Salmonella Spp ◽  
Fecal Samples ◽  
Cold Room ◽  
Tank Water ◽  
Campylobacter Spp

This study was done to evaluate the degree of contamination of cooler-ready hog carcasses and the slaughterhouse environment by Salmonella spp., Campylobacter spp. and Yersinia enterocolitica. Samples from diaphragms and feces were collected from 200 market hogs in a Quebec slaughterhouse. Scalding-tank water and environmental swabs were also collected in the slaughterhouse. Specimens were tested for the presence of Salmonella spp., Y. enterocolitica, and Campylobacter spp. Salmonella spp. were isolated from 45 (10%) of 448 samples. The distribution of the isolates were slaughtering floor (8.9%), feces (80.2%), cold-room floor (4.4%), and diaphragms (6.7%). Campylobacter spp. were isolated from 247 (61.7%) of 400 specimens, and C. coli, C. jejuni, and C. laridis accounted for 97%, 2%, and 1% of isolates, respectively. Ninety-nine percent of fecal samples were positive for the presence of C. coli. Y. enterocolitica was found in 42 (9.3%) of the 448 specimens. Of these, 85.7%, 11.9%, and 2.4% of the isolates came from fecal, diaphragm, and cold-room floor samples, respectively. Neither Salmonella spp. nor Y. enterocolitica were isolated from scalding-tank water.

scholarly journals Saúde Animal, Biossegurança e Segurança dos alimentos

2018 ◽  
Vol 67 (Supplement) ◽  
pp. 131-135
Author(s):  
R. Potes ◽  
M.A. Laranjo ◽  
L. Elias
Keyword(s):  
Listeria Monocytogenes ◽  
Toxoplasma Gondii ◽  
Yersinia Enterocolitica ◽  
Trichinella Spiralis ◽  
Taenia Solium ◽  
Salmonella Spp ◽  
Campylobacter Spp

Alguns perigos que podem ameaçar a saúde dos consumidores de carne de porco e de produtos processados estão relacionados com a saúde animal ou com a manipulação da matéria-prima desde o abate dos animais, a transformação e até ao consumo. Entre os perigos químicos consideram-se as substâncias com efeitos anabólicos, os resíduos de substâncias medicamentosas e os contaminantes químicos, alguns com origem na produção animal ou produzidos durante o processamento. No que se refere aos perigos biológicos, podem encontrar-se alguns parasitas como Toxoplasma gondii, Trichinella spiralis e Taenia solium e também bactérias como Salmonella spp, Campylobacter spp., Yersinia enterocolitica, Listeria monocytogenes. As características físico-químicas da carne fresca facilitam o crescimento de diversos microrganismos, para além dos mencionados anteriormente, o que a torna altamente perecível. Os diferentes processos de transformação desta matéria-prima, não só permitem obter diferentes alimentos para o consumidor como constituem formas de aumentar a sua vida útil. Entre os processos de transformação incluem-se a secagem, a fermentação e a cura. Em Portugal há uma grande variedade de produtos cárneos tais como Chouriço, Paio, Presunto, Salsichão e Catalão, muitos dos quais são fabricados segundo as técnicas tradicionais das diferentes regiões de onde são originários. Alguns destes produtos têm sido estudados na Universidade de Évora. Apresentaremos alguns resultados que mostram o efeito favorável de vários processos de transformação no controlo de alguns dos perigos biológicos mencionados.

Occurrence of Zoonotic Bacteria in Retail Game Meat in Japan with Special Reference to Erysipelothrix

1997 ◽  
Vol 60 (3) ◽  
pp. 328-331 ◽  
Author(s):  
YOKO KANAI ◽  
HIDEKI HAYASHIDANI ◽  
KEN-ICHI KANEKO ◽  
MASUO OGAWA ◽  
TOSHIO TAKAHASHI ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Wild Boar ◽  
Yersinia Enterocolitica ◽  
The Other ◽  
Salmonella Spp ◽  
Serotype 1 ◽  
Meat Samples ◽  
Zoonotic Bacteria ◽  
Highly Virulent ◽  
Campylobacter Spp

From December 1993 to March 1994, a total of 93 wild boar and 30 deer meat samples were purchased from 13 retail shops and examined for the presence of species of Erysipelothrix, Yersinia, Listeria, Salmonella and Campylobacter. Erysipelothrix spp. were isolated from 41 wild boar and 15 deer samples. These isolates were identified as 13 serotypes. Serotypes 6 and 2 were the most predominant. Of 17 isolates examined, 14 isolates were highly virulent for mice. The bacteria of the same serotype were isolated repeatedly from the samples purchased in 4 of the shops. Yersinia enterocolitica was isolated from 36 wild boar and 10 deer samples; however, all isolates of Y. enterocolitica did not have pathogenic properties. Listeria monocytogenes was isolated from 5 wild boar samples. The isolates were identified as serotype 1/2c and 4b. Salmonella spp. were isolated from 2 wild boar samples; one isolate was identified as S. typhimurium and the other was untypable. Campylobacter spp. were not detected.

scholarly journals Brotes de enfermedades transmitidas por los alimentos estudiados mediante técnicas moleculares

2017 ◽  
Vol 19 (5) ◽  
pp. 671-678
Author(s):  
Elkin Enrique Hernández Porras ◽  
Liliana Elisa Rosero Torres ◽  
Eliana Liseth Parra Barrera ◽  
Jaime Alberto Guerrero Montilla ◽  
Adriana Leonor Gómez Rubio ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Yersinia Enterocolitica ◽  
Salmonella Spp

Objetivo Aplicar una técnica de reacción en cadena de la polimerasa (PCR) múltiple en tiempo real para la detección de Salmonella spp., Listeria monocytogenes y Yersinia enterocolitica, como herramienta de apoyo diagnóstico en la vigilancia de brotes de enfermedad transmitida por alimentos.Materiales y Métodos Se aplicó la metodología molecular en muestras clínicas provenientes de individuos que estaban asociados a brotes de enfermedad transmitida por alimentos de dos departamentos de Colombia. Los resultados se compararon con los datos arrojados por la metodología convencional de cultivo. Adicionalmente a los aislamientos obtenidos se les evaluó relación clonal mediante la técnica de electroforesis de campo pulsado (PFGE).Resultados Se determinó un total de 123 casos de enfermedad transmitida por alimentos de los cuales 45 muestras biológicas fueron confirmadas por laboratorio y 88 mediante nexo epidemiológico. La metodología molecular detectó 35/45 muestras positivas frente a 17/45 muestras positivas detectadas mediante la metodología convencional. La PFGE demostró relación clonal en cada brote.Conclusión Los resultados del estudio demuestran la aplicabilidad de la técnica molecular como herramienta útil de apoyo diagnóstico en la caracterización de brotes de enfermedad transmitida por alimentos, permitiendo una respuesta oportuna y confiable.

Contamination of Pork Carcasses during Slaughter, Fabrication, and Chilled Storage

1995 ◽  
Vol 58 (9) ◽  
pp. 993-997 ◽  
Author(s):  
JAIME J. SAIDE-ALBORNOZ ◽  
C. LYNN KNIPE ◽  
ELSA A. MURANO ◽  
GEORGE W. BERAN
Keyword(s):  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Yersinia Enterocolitica ◽  
Pathogenic Bacteria ◽  
Linear Increase ◽  
Human Consumption ◽  
Refrigerated Storage ◽  
Salmonella Spp ◽  
Chilled Storage ◽  
Pathogenic Contamination

In an attempt to determine points of greatest pathogenic contamination of pork, the prevalence of five pathogens was determined on pork carcasses at specific points during slaughter, fabrication, and refrigerated storage. Pork carcass and loin surfaces were swabbed at three hog slaughtering plants. Carcasses were swabbed after singeing, after the final wash of the slaughter process, and after 24 h of chilled storage. Boneless loins were swabbed after trimming and deboning, but before packaging. Also, vacuum-packaged loins were sampled after 36 days of storage at 2°C. Staphylococcus aureus, Salmonella spp., and Listeria monocytogenes were the most prevalent. S. aureus isolates showed a significant linear increase (P = 0.0399) from slaughter to fabrication processes, with the highest numbers detected after 24 h of refrigerated storage. Trimming fat from surfaces of pork loins reduced the number of initial S. aureus counts, but there was no further reduction after 36 days of refrigerated storage. Salmonella were isolated primarily from pork before fabrication and refrigerated storage. A continuous reduction in the numbers of Salmonella isolates was detected from the point of singeing to the point of fabrication. No Salmonella were isolated from vacuum-packaged pork stored for 36 days at 2°C. The relatively higher prevalence of the psychrotrophic pathogenic bacteria L. monocytogenes and Yersinia enterocolitica in vacuum-packaged pork loins after 36 days of storage at 2°C indicates the need for proper cooking and handling of meats prior to human consumption.

scholarly journals Presence and Characterization of Zoonotic Bacterial Pathogens in Wild Boar Hunting Dogs (Canis lupus familiaris) in Tuscany (Italy)

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1139
Author(s):  
Giovanni Cilia ◽  
Filippo Fratini ◽  
Barbara Turchi ◽  
Valentina Virginia Ebani ◽  
Luca Turini ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Wild Boar ◽  
Yersinia Enterocolitica ◽  
Canis Lupus ◽  
Canis Lupus Familiaris ◽  
Salmonella Spp ◽  
Work Activity ◽  
Hunting Dogs ◽  
Rectal Swabs

Domestic dogs (Canis lupus familiaris) used for wild boar (Sus scrofa) hunting may represent incidental hosts for several zoonotic pathogens. This investigation aimed to evaluate the presence of anti-Leptospira antibodies and the occurrence, antimicrobial resistance, and virulence of Salmonella spp., Yersinia enterocolitica, and Listeria monocytogenes in sera and rectal swabs collected from 42 domestic hunting dogs in the Tuscany region (Italy). Regarding Leptospira, 31 out of 42 serum samples (73.8%) were positive and serogroup Pomona was the most detected (71.4%) at titers between 1:100 and 1:400. Four Salmonella isolates (9.52%) were obtained, all belonging to serotype Infantis; two of them showed antimicrobial resistance to streptomycin, while pipB and sopE presence was assessed in all but one isolate. Concerning Yersinia enterocolitica, seven isolates (16.7%) were obtained, six belonging to biotype 1 and one to biotype 4. Resistance to amoxicillin–clavulanic acid, cephalothin, and ampicillin was detected. Biotype 4 presented three of the virulence genes searched (ystA, ystB, inv), while isolates of biotype 1 showed only one gene. No Listeria monocytogenes was isolated from dog rectal swabs. The results suggest that hunting dogs are exposed to different bacterial zoonotic agents, potentially linked to their work activity, and highlight the possible health risks for humans.

scholarly journals Prévalence de Salmonella, Listeria monocytogenes, Campylobacter spp., Yersinia enterocolitica et Cryptosporidium spp. dans du lait en vrac, des matières fécales de vaches et des effluents de fermes laitières à Trinidad

1996 ◽  
Vol 49 (4) ◽  
pp. 303-309
Author(s):  
A.A. Adesiyun ◽  
L.A. Webb ◽  
H. Romain ◽  
J.S. Kaminjolo
Keyword(s):  
Listeria Monocytogenes ◽  
Yersinia Enterocolitica ◽  
Salmonella Spp ◽  
Cryptosporidium Spp ◽  
Campylobacter Spp

La prévalence de Listeria, Salmonella, Campylobacter, Yersinia et Cryptosporidium dans du lait en vrac, les matières fécales des vaches laitières et des effluents à lait des fermes laitières de l'île de la Trinité, a été evaluée. Des 177 échantillons de lait en vrac testés, 3 (1,7 %), 3 (1,7 %) et 2 (1,2 %) étaient positifs respectivement pour Listeria monocytogenes, Salmonella spp. et Yersinia enterocolitica mais négatifs pour Campylobacter spp. et Cryptosporidium spp. Des 333 échantillons fécaux testés, L. monocytogenes, Campylobacter spp. et Cryptosporidium spp. étaient présents dans respectivement 11 (3,3 %), 19 (5,7 %) et 7 (2,1 %) des cas. Ces taux étaient significativement (P < 0,05; chi2) plus élevés, que le taux de détection (0 %) pour Yersinia spp. Salmonella spp. a été detectée dans 3 (0,9 %) échantillons fécaux. Des 168 échantillons prélevés des effluents à lait, Campylobacter spp. était présent dans 7 (4,2 %) échantillons alors que Salmonella spp. l'était dans seulement 1 (0,6 %) échantillon. Aucun échantillon n'était positif pour Yersinia spp. et Listeria spp. (différence significative, P<= 0,05; chi2). En conclusion, les cinq espèces entero-pathogènes testées étaient présentes dans les fermes laitières de l'île de la Trinité.

scholarly journals Prevalence, Virulence and Antimicrobial Susceptibility of Salmonella spp., Yersinia enterocolitica and Listeria monocytogenes in European Wild Boar (Sus scrofa) Hunted in Tuscany (Central Italy)

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 93
Author(s):  
Giovanni Cilia ◽  
Barbara Turchi ◽  
Filippo Fratini ◽  
Stefano Bilei ◽  
Teresa Bossù ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Wild Boar ◽  
Yersinia Enterocolitica ◽  
Virulence Factor ◽  
Central Italy ◽  
Human Pathogens ◽  
Salmonella Spp ◽  
European Wild Boar ◽  
Amoxicillin Clavulanic Acid ◽  
Virulence Factor Genes

Wild boar is an animal the population of which constantly increases in Europe. This animal plays an important role as a reservoir for several pathogens, including three of the most important zoonoses: salmonellosis, yersiniosis and listeriosis. The aim of this investigation was to evaluate the occurrence of antimicrobial-resistant and virulence factor genes of Salmonella spp., Yersinia enterocolitica and Listeria monocytogenes isolated from wild boar in Tuscany (Central Italy). During two consequent hunting seasons (2018/2019 and 2019/2020), rectal swabs, spleens and livers were collected from 287 hunted wild boar to isolate strains. Each isolate was tested to investigate its antimicrobial resistance and to detect virulence factor genes by PCR. Eighteen Salmonella strains (6.27%) were isolated. Of these, 66.7% were resistant to streptomycin, 13.4% to cephalothin, 6.67% to imipenem and one isolate (6.67%) was resistant simultaneously to five antimicrobials. Moreover, the most detected genes were sopE (73.4%), pipB (66.7%), sodCI (53.3%), spvR and spvC (46.7%). In total, 54 (17.8%) Yersinia enterocolitica were isolated; of them, 26 (48.1%), 9 (16.7%), 17 (31.5%), 1 (1.85%) and 1 (1.85%) belonged to biotypes 1, 2, 3, 4 and 5, respectively. All strains (100%) demonstrated resistance to cephalothin and 70.4% to amoxicillin-clavulanic acid, 55.6% to ampicillin, and 37.0% to cefoxitin. Additionally, the most detected genes were ystA (25.9%), inv (24.1%), ail (22.2%), ystB (18.5%) and virF (14.8%). Finally, only one Listeriamonocytogenes isolate (0.35%) was obtained, belonging to serogroup IVb, serovar 4b, and it was found to be resistant to cefoxitin, cefotaxime and nalidixic acid. The results highlighted the role of wild boar as a carrier for pathogenic and antimicrobial-resistant Salmonella spp., Yersinia enterocolitica and Listeria monocytogens, representing a possible reservoir for domestic animals and human pathogens.

Caracterización de la leche y queso artesanal de la región de Ojos Negros Baja California, México.

2020 ◽  
Vol 11 (2) ◽  
pp. 553-564
Author(s):  
Laura E. Silva-Paz ◽  
Gerardo E. Medina-Basulto ◽  
Gilberto López-Valencia ◽  
Martin F. Montaño-Gómez ◽  
Rafael Villa-Angulo ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Baja California ◽  
Salmonella Spp ◽  
Buenas Prácticas

La comunidad de Ojos Negros está ubicada en el municipio de Ensenada Baja California, México. Desde 1930, los residentes locales fabrican un queso artesanal muy apreciado en la región; sin embargo, la leche cruda y el queso nunca han sido analizados por la calidad microbiológica y de higiene del producto final. El objetivo del presente estudio fue evaluar la calidad microbiológica, física y química de la leche cruda utilizada para producir queso y el queso artesanal producido en las 22 unidades de producción individuales. Se tomaron muestras de queso y leche de las unidades de producción para realizar pruebas microbiológicas. Se realizaron determinaciones físicas y químicas de proteínas, grasas y lactosa utilizando un analizador LACTOSCAN-S. Los resultados del análisis de la leche mostraron un contenido de proteína (33,11 g/l) y grasa (39,89 g/l) dentro de los parámetros de la normatividad. Para la calidad microbiológica de la leche, los resultados del recuento de mesófilos aeróbicos mostraron un cumplimiento del 64% con las regulaciones; sin embargo, el mismo conteo de mesófilos aeróbicos en las muestras de queso resultó en solo el 4% de cumplimiento con las regulaciones. No hubo detección de Salmonella spp. o Listeria monocytogenes en cualquiera de las muestras de leche o de queso probadas. Se deben incorporar buenas prácticas sanitarias y de fabricación para mejorar la calidad sanitaria y de higiene para la producción de queso artesanal en la comunidad de Ojos Negros.

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