Comparison of Individual, Pooled, and Composite Fecal Sampling Methods for Detection of Salmonella on U.S. Dairy Operations

2012 ◽  
Vol 75 (9) ◽  
pp. 1562-1571 ◽  
Author(s):  
J. E. LOMBARD ◽  
A. L. BEAM ◽  
E. M. NIFONG ◽  
C. P. FOSSLER ◽  
C. A. KOPRAL ◽  
...  
Keyword(s):  
Fecal Sample ◽  
Animal Health ◽  
Study Data ◽  
Dairy Herds ◽  
Sample Type ◽  
Infection Status ◽  
Department Of Agriculture ◽  
Fecal Samples ◽  
Pooled Samples ◽  
Culture Positive

The objectives of this study were to estimate the prevalence of Salmonella for individual, pooled, and composite fecal samples and to compare culture results from each sample type for determining herd Salmonella infection status and identifying Salmonella serovar(s). During the U.S. Department of Agriculture National Animal Health Monitoring System Dairy 2007 study, data and samples were collected from dairy operations in 17 major dairy states. As part of the study, composite fecal samples (six per operation) were collected from cow areas, such as holding pens, alleyways, and lagoons, where manure accumulates. Fecal samples also were collected from individual cows (35 per operation), and fecal sample pools were created by combining samples from 5 cows (7 per operation). A total of 1,541 composite fecal samples were collected from 260 operations in 17 states, and 406 (26.3%) of these samples were culture positive for Salmonella. Among the 116 operations for which all three sample types were obtained, 41.4% (48 operations) were Salmonella culture positive based on individual samples, 39.7% (46 operations) were positive based on pooled samples, and 49.1% (57 operations) were positive based on composite fecal samples. Relative to individual samples, the sensitivity of composite fecal samples for determining herd infection status was 85.4% and the sensitivity of pooled fecal samples was 91.7%. On 33.6% of operations (39 of 116), Salmonella was cultured from all three fecal sample types (individual, pooled, and composite), and 20 (51.3%) of these operations had exactly the same serovar in all three sample types. Use of composite fecal samples is less costly and time-consuming than use of individual or pooled samples and provides similar results for detecting the presence and identifying serovars of Salmonella in dairy herds. Therefore, composite sampling may be an appropriate alternative to culture of individual samples when assessing Salmonella status in dairy herds.

scholarly journals Three-year monitoring of paratuberculosis in dairy cattle by pooled faecal culture and individual prevalence estimation

2018 ◽  
Vol 18 (1) ◽  
pp. 99-111
Author(s):  
Alberto Prieto ◽  
José Manuel Díaz-Cao ◽  
Pablo Díaz ◽  
Ana Pérez-Creo ◽  
Gonzalo López-Lorenzo ◽  
...  
Keyword(s):  
First Year ◽  
Dairy Herds ◽  
Bayesian Approaches ◽  
Faecal Culture ◽  
Control Programmes ◽  
Second Year ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Pooled Samples ◽  
Culture Positive

Abstract The objective of this study was to assess the implementation of a three-year Mycobacterium avium subsp. paratuberculosis monitoring programme using pooled faecal culture in small and mediumsized dairy herds to classify them as infected or non-infected and apply proper hygiene and biosecurity measures. Over a three-year period, 35 dairy herds were analysed annually by faecal culture of ten pooled samples. In addition, proper hygiene and biosecurity protocols were implemented in the farms after the first testing round. Considering a herd as infected with at least one culture positive in any of the three years, the accumulated percentage of infected herds was 25.7%, 40% and 45.7%, for each year respectively. Assuming that all infected herds had been detected at the end of the study, the percentage of infected herds detected each year was 56.25% and 87.5% for the first and second year, respectively. Using frequentist and Bayesian approaches, the estimated individual prevalence revealed a downward trend from 3.30-3.65% in the first year to 1.66-1.86% in the third year. The results of this study indicate that pooled faecal culture allowed for proper classification of the herds and can be a useful tool for monitoring dairy herds against paratuberculosis. In addition, statistical analysis of pooled faecal culture results can be used to evaluate the evolution of individual prevalence in the population and therefore the function of the implemented control programmes.

Effects of Farm Manure-Handling Practices on Escherichia coli O157 Prevalence in Cattle

1997 ◽  
Vol 60 (4) ◽  
pp. 363-366 ◽  
Author(s):  
DALE D. HANCOCK ◽  
DANIEL H. RICE ◽  
DONALD E. HERRIOTT ◽  
THOMAS E. BESSER ◽  
ERIC D. EBEL ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dairy Herds ◽  
Escherichia Coli O157 ◽  
Forage Crops ◽  
Fecal Samples ◽  
Herd Prevalence ◽  
E Coli ◽  
High Prevalence ◽  
Handling Practices ◽  
Culture Positive

Thirty-six dairy herds in Idaho, Oregon, and Washington were selected on the basis of cattle housing and manure-handling practices. Approximately 60 fecal samples from heifers were collected monthly in each herd for 6 months and cultured for Escherichia coli O157. One hundred seventy-nine of 12,664 (1.41%) individual fecal samples from 27 of the 36 herds (75%) were culture positive for E. coli O157. Within-herd prevalence ranged from 0% to 5.5% with a strong clustering toward the lower end of this range. A tendency was observed for herds to maintain either a relatively low or high prevalence of E. coli O157. Prevalence of E. coli O157 was similar in herds which housed heifers in dry lots and on pasture with and without application of manure. Also, application of manure to cattle forage crops was not associated with the prevalence of E. coli O157 in dairy herds.

Salmonella in Dairy Operations in the United States: Prevalence and Antimicrobial Drug Susceptibility

2005 ◽  
Vol 68 (4) ◽  
pp. 696-702 ◽  
Author(s):  
D. M. BLAU ◽  
B. J. McCLUSKEY ◽  
S. R. LADELY ◽  
D. A. DARGATZ ◽  
P. J. FEDORKA-CRAY ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
Antimicrobial Agents ◽  
Animal Health ◽  
Dairy Farm ◽  
The United States ◽  
Drug Susceptibility ◽  
National Study ◽  
Dairy Herds ◽  
Antimicrobial Drugs ◽  
Fecal Samples

Salmonella serotypes are important foodborne pathogens of humans that can be acquired through consumption of contaminated meat and dairy products. Salmonella infection also can be a significant animal health issue. As part of a national study of U.S. dairy operations conducted between March and September 2002, fecal samples were collected from representative cows in 97 dairy herds in 21 states and were cultured to determine the prevalence of Salmonella shedding. Salmonella was recovered from the feces of at least one cow in 30.9% of the herds. Overall, 7.3% of fecal samples were culture positive for Salmonella. The three most frequently recovered serotypes were Salmonella Meleagridis (24.1%), Salmonella Montevideo (11.9%), and Salmonella Typhimurium (9.9%). The susceptibilities of Salmonella isolates recovered were determined using a panel of 16 antimicrobial drugs. Salmonella isolates recovered from dairy cows had relatively little resistance to these antimicrobial agents; 83.0% of the isolates were susceptible to all antimicrobials tested. This study provides updated information on the prevalence and susceptibility patterns of Salmonella in dairy herds and on cow and herd characteristics. These data contribute to our understanding of the ecology of Salmonella in the dairy farm environment.

scholarly journals Suitability of Nasal and Deep Nasopharyngeal Swab Sampling of Calves in the Mycoplasma bovis Control Program

2021 ◽  
Vol 8 ◽  
Author(s):  
Tarja Pohjanvirta ◽  
Nella Vähänikkilä ◽  
Vera Talvitie ◽  
Sinikka Pelkonen ◽  
Tiina Autio
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Animal Health ◽  
Control Program ◽  
Test Method ◽  
Diagnostic Tools ◽  
Effective Vaccine ◽  
Dairy Herds ◽  
Mycoplasma Bovis ◽  
Infection Status

Mycoplasma bovis is an important cattle pathogen affecting animal health, welfare, and productivity. The main disease syndromes are mastitis, pneumonia, and otitis media in young stock, as well as arthritis. Response to antibiotic treatment is poor and no effective vaccine is available. Asymptomatic carriers are common and usually harbor the organism in the airways or mammary glands. Purchase of carrier animals is a major risk for the introduction of infection into naive herds. Following the detection of M. bovis in Finland in 2012, a voluntary control program was established. It aims to prevent the spread of the infection and to help farms attain certification of a low M. bovis risk. Among the diagnostic tools in the program, nasal swabs (NS) from young calves have been tested for M. bovis to indicate the infection status of the herd. In this study, we assessed the suitability of this test method. We analyzed the effectiveness of NS and deep nasopharyngeal swabs (NP) to detect M. bovis in pneumonic and healthy calves in dairy herds recently infected with M. bovis. In pneumonic calves, NP sampling followed by culture and real-time PCR demonstrated a proportion of positive agreement (PPA) of 0.91 compared with bronchoalveolar lavage (BAL), whereas NS showed only 0.5 PPA compared with BAL. Among healthy dairy calves, overall M. bovis prevalence in NS was 29.6%. The highest rate of shedding (43%) occurred in calves 31–60 days old. At the calf level, M. bovis prevalence in NP samples was 47% compared with 33% in NS samples among the 284 studied calves. However, at the herd level, NS sampling classified 51 out of 54 herds with a positive infection status as infected, whereas in NP sampling, the respective figure was 43 out of 54 herds (p = 0.061). In conclusion, NS sampling from calves under 6 months of age and analyzed by real-time PCR is a cost-efficient method for a control program to detect M. bovis in dairy herds, even if no M. bovis mastitis has been detected in the herd. For pneumonic calves, we recommend only NP or BAL sampling.

scholarly journals Development of a Combined Selection and Enrichment PCR Procedure for Clostridium botulinum Types B, E, and F and Its Use To Determine Prevalence in Fecal Samples from Slaughtered Pigs

2001 ◽  
Vol 67 (10) ◽  
pp. 4781-4788 ◽  
Author(s):  
Maria Dahlenborg ◽  
Elisabeth Borch ◽  
Peter Rådström
Keyword(s):  
Sample Preparation ◽  
Fecal Sample ◽  
Clostridium Botulinum ◽  
Geographical Location ◽  
Type B ◽  
Fecal Samples ◽  
Pcr Assays ◽  
Combined Selection ◽  
Spore Load ◽  
Slaughtered Pigs

ABSTRACT A specific and sensitive combined selection and enrichment PCR procedure was developed for the detection of Clostridium botulinum types B, E, and F in fecal samples from slaughtered pigs. Two enrichment PCR assays, using the DNA polymeraserTth, were constructed. One assay was specific for the type B neurotoxin gene, and the other assay was specific for the type E and F neurotoxin genes. Based on examination of 29 strains of C. botulinum, 16 strains of other Clostridium spp., and 48 non-Clostridium strains, it was concluded that the two PCR assays detect C. botulinum types B, E, and F specifically. Sample preparation prior to the PCR was based on heat treatment of feces homogenate at 70°C for 10 min, enrichment in tryptone-peptone-glucose-yeast extract broth at 30°C for 18 h, and DNA extraction. The detection limits after sample preparation were established as being 10 spores per g of fecal sample for nonproteolytic type B, and 3.0 × 103 spores per g of fecal sample for type E and nonproteolytic type F with a detection probability of 95%. Seventy-eight pig fecal samples collected from slaughter houses were analyzed according to the combined selection and enrichment PCR procedure, and 62% were found to be PCR positive with respect to the type B neurotoxin gene. No samples were positive regarding the type E and F neurotoxin genes, indicating a prevalence of less than 1.3%. Thirty-four (71%) of the positive fecal samples had a spore load of less than 4 spores per g. Statistical analysis showed that both rearing conditions (outdoors and indoors) and seasonal variation (summer and winter) had significant effects on the prevalence of C. botulinum type B, whereas the effects of geographical location (southern and central Sweden) were less significant.

scholarly journals Effect of Environmental Factors and Influence of Rumen and Hindgut Biogeography on Bacterial Communities in Steers

2010 ◽  
Vol 77 (1) ◽  
pp. 258-268 ◽  
Author(s):  
Gustavo A. Romero-Pérez ◽  
Kim H. Ominski ◽  
Tim A. McAllister ◽  
Denis O. Krause
Keyword(s):  
Ambient Temperature ◽  
Bacterial Communities ◽  
Volatile Fatty Acids ◽  
Principal Component ◽  
Population Diversity ◽  
Access Time ◽  
Sample Type ◽  
Bacterial Populations ◽  
Fecal Samples ◽  
Alfalfa Silage

ABSTRACTFeces from cattle production are considered important sources of bacterial contamination of food and the environment. Little is known about the combined effects of arctic temperatures and fodder tannins on rumen and hindgut bacterial populations. Individual rumen liquor and rectal fecal samples from donor steers fed either alfalfa silage or sainfoin (Onobrychis viciifoliaScop.) silage and waterad libitumwere collected weekly on the first three sampling days and fortnightly afterwards. The daily ambient temperatures were registered and averaged to weekly mean temperatures. Steers fed sainfoin silage had lower (P< 0.05) concentrations of branched-chain volatile fatty acids (VFA) than those fed alfalfa silage. All VFA concentrations were higher (P< 0.001) in rumen liquor samples than in fecal samples. The interaction of sample type and diet showed a significant effect (P< 0.05) on the proportions of the bacterial community that were from the phylaProteobacteriaandVerrucomicrobia.Ambient temperature had an indirect effect (P< 0.05) on the phylumFirmicutes, as it affected its proportional balance. The bacterial population diversity in samples appeared to decrease concurrently with the ambient temperature. The phylumFirmicutesexplained the first principal component at 64.83 and 42.58% of the total variance in rumen liquor and fecal samples, respectively. The sample type had a larger effect on bacterial communities than diet and temperature. Certain bacterial populations seemed to be better adapted than others to environmentally adverse conditions, such as less access time to nutrients due to higher motility and rate of passage of digesta caused by extreme temperatures, or antimicrobials such as tannins, possibly due to an influence of their biogeographical location within the gut.

scholarly journals The latest FAD – Faecal antibody detection in cattle. Protocol and results from three UK beef farms naturally infected with gastrointestinal nematodes

Parasitology ◽  
2018 ◽  
Vol 146 (1) ◽  
pp. 89-96 ◽  
Author(s):  
A. S. Cooke ◽  
K. A. Watt ◽  
E. R. Morgan ◽  
J. A. J. Dungait
Keyword(s):  
Animal Health ◽  
Nematode Species ◽  
Gastrointestinal Nematodes ◽  
Vital Role ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Fecal Samples ◽  
Gastrointestinal Health ◽  
Immunological Protection

AbstractAntibodies at gastrointestinal mucosal membranes play a vital role in immunological protection against a range of pathogens, including helminths. Gastrointestinal health is central to efficient livestock production, and such infections cause significant losses. Fecal samples were taken from 114 cattle, across three beef farms, with matched blood samples taken from 22 of those animals. To achieve fecal antibody detection, a novel fecal supernatant was extracted. Fecal supernatant and serum samples were then analysed, using adapted enzyme-linked immunosorbent assay protocols, for levels of total immunoglobulin (Ig)A, IgG, IgM, andTeladorsagia circumcincta-specific IgA, IgG, IgM and IgE (in the absence of reagents for cattle-specific nematode species). Fecal nematode egg counts were conducted on all fecal samples. Assays performed successfully and showed that IgA was the predominant antibody in fecal samples, whereas IgG was predominant in serum. Total IgA in feces and serum correlated within individuals (0.581,P= 0.005), but other Ig types did not. Results support the hypothesis that the tested protocols are an effective method for the non-invasive assessment of cattle immunology. The method could be used as part of animal health assessments, although further work is required to interpret the relationship between results and levels of infection and immunity.

scholarly journals Animal health and welfare planning improves udder health and cleanliness but not leg health in Austrian dairy herds

2015 ◽  
Vol 98 (10) ◽  
pp. 6801-6811 ◽  
Author(s):  
Lukas Tremetsberger ◽  
Christine Leeb ◽  
Christoph Winckler
Keyword(s):  
Animal Health ◽  
Dairy Herds ◽  
Udder Health

scholarly journals Effects of a single initial and delayed release implant on arrival compared with a non-coated initial implant and a non-coated terminal implant in heifers fed across various days on feed

2019 ◽  
Vol 3 (4) ◽  
pp. 1182-1193 ◽  
Author(s):  
Zachary K Smith ◽  
Ben P Holland ◽  
Alyssa B Word ◽  
Grant I Crawford ◽  
Wade N Nichols ◽  
...  
Keyword(s):  
Growth Performance ◽  
Animal Health ◽  
Feed Ratio ◽  
United States Department ◽  
Initial Body Weight ◽  
Department Of Agriculture ◽  
Live Animal ◽  
Trenbolone Acetate ◽  
The Mean ◽  
Estradiol 17Β

Abstract Two experiments evaluated the effect of implant number, type, and total steroidal dose on live animal performance and carcass traits in heifers fed for three different days on feed (DOF). In experiment 1, heifers (n = 3,780; 70 heifers/pen and 9 pens/treatment; initial body weight [BW] = 309 kg) were used in a 2 × 3 factorial arrangement of treatments. Factors were as follows: 1) implant (all from Merck Animal Health, De Soto, KS): 200 mg trenbolone acetate (TBA) and 20 mg estradiol-17β (E2) administered on arrival (SINGLE), or 80 mg TBA and 8 mg E2 administered on arrival followed by 200 mg TBA and 20 mg E2 after approximately 90 d (REPEATED) and 2) duration of DOF: harvested after approximately 172, 193, and 214. In experiment 2, heifers (n = 3,719; 65 to 70 heifers/pen and 9 pens/treatment; initial BW = 337 kg) were used with the same factors as experiment 1, except DOF were 150, 171, and 192. No implant × DOF interaction (P ≥ 0.06) was noted for any performance parameters in either experiment. Heifers administered REPEATED had improved (P ≤ 0.05) live gain to feed ratio (G:F) and carcass-adjusted G:F and tended (P = 0.09) to have greater hot carcass weight (HCW) in experiment 1. Increasing DOF resulted in greater (P ≤ 0.01) live and carcass-adjusted final BW and decreased (P = 0.01) live ADG in experiment 1. As DOF increased, HCW, HCW gain, and dressing% (P ≤ 0.01) increased in experiment 1. The mean carcass transfer was 79.6% across the 42 d terminal window in experiment 1. In experiment 2, REPEATED had improved (P = 0.03) carcass-adjusted G:F compared with SINGLE, but HCW was not different (P = 0.36) between treatments. Increased DOF resulted in greater (P ≤ 0.01) final live and carcass-adjusted BW, decreased (P ≤ 0.01) live and carcass-adjusted ADG, and poorer (P ≤ 0.01) live and carcass-adjusted G:F in experiment 2. In experiment 2, dressing percentage was greater (P = 0.02) in REPEATED compared with SINGLE. Heifers given SINGLE had greater (P = 0.01) back fat and estimated empty body fat (EBF), whereas REPEATED had fewer (P = 0.01) Yield Grade 4 carcasses and greater (P = 0.01) longissimus muscle (LM) area. Increased DOF resulted in greater (P ≤ 0.04) HCW, HCW gain, dressing%, back fat, LM area, marbling, EBF%, and United States Department of Agriculture (USDA) Prime-grading carcasses, Yield Grade 4 and 5, and over 454-kg carcasses in experiment 2. Carcass ADG and carcass transfer indicate a 0.70 kg carcass ADG between 150 and 192 DOF, resulting in an average carcass transfer of 72.2% in experiment 2. Although feedlot growth performance and HCW did not differ between the implant regimens tested, increasing DOF resulted in decreased live growth performance while increasing the proportion of USDA prime carcasses and HCW.

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