scholarly journals Research Progress in Determination Methods of Formaldehyde Content in Indoor Air

2021 ◽  
Vol 2 (4) ◽  
Author(s):  
Bowei Yao ◽  
Hairui Wang ◽  
Wei Zhang ◽  
Lili Feng
Keyword(s):  
Indoor Air ◽  
Rapid Detection ◽  
Detection Method ◽  
Detection Methods ◽  
Research Progress ◽  
Formaldehyde Content ◽  
Advantages And Disadvantages ◽  
Determination Methods ◽  
Phenol Reagent ◽  
Detection Principle

The detection methods of formaldehyde content in indoor air, including traditional laboratory detection methods (AHMT spectrophotometry, phenol reagent spectrophotometry, acetyl acetone spectrophotometry and gas chromatography) and rapid detection methods (electrochemical sensor method, photoelectric spectrophotometry, etc.), were introduced and described. This paper systematically analyzes and compares the detection principle, applicable environment medium, detection flux and the advantages and disadvantages of each detection method. The future detection methods of formaldehyde content in indoor air were prospected.

A Survey on Detecting Methods for Harmonic of Power System

2012 ◽  
Vol 614-615 ◽  
pp. 907-910
Author(s):  
Xue Song Zhou ◽  
Guang Zhu Chen ◽  
You Jie Ma
Keyword(s):  
Power System ◽  
Reactive Power ◽  
Detection Method ◽  
Response Speed ◽  
Development Trend ◽  
Detection Methods ◽  
Detection Accuracy ◽  
Harmonic Detection ◽  
Advantages And Disadvantages ◽  
Power System Harmonics

This paper describes Detecting Methods for Harmonics of power system has been considered one of the serious harms for power system. The research of harmonics has obtained high attention among people. The researches of harmonics detection have many methods, such as Based on Fryze theory of harmonic power detection, Instantaneous reactive power theory detection method, Fourier Transformation harmonic detection method, wavelet transform detection method, neural networks harmonic detection method etc. Aiming at harmonic detection, the different detection methods of power system harmonics are summarized and compared. This paper reviews the existing harmonic detection methods, and discuss their advantages and disadvantages in terms of detection accuracy and response speed, and finally summarizes the development trend of the harmonic detection method.

scholarly journals Development of a rapid detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65

2020 ◽  
Author(s):  
Yuhua Li ◽  
Haoran Li ◽  
Xiaoxiao Song ◽  
Hao Zhang ◽  
Yujuan Duan ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Trichomonas Vaginalis ◽  
Detection Method ◽  
Reaction System ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Detection Methods ◽  
Adhesion Protein ◽  
Loop Mediated Isothermal Amplification ◽  
Pcr Targeting

Abstract Background: Trichomoniasis resulting from Trichomonas vaginalis (T. vaginalis) has been considered as a commonly seen disease with the transmission way of sex. At present, the detection methods of T. vaginalis mainly include wet mount microscopy, culture, PCR, immunofluorescence and ELISA. However, all of these detection methods exist shortcomings.Methods: In this study, a loop-mediated isothermal amplification (LAMP) assay that targeted the species-specific sequence of adhesion protein 65 (AP65) gene had been conducted to detect T. vaginalis. The optimum reaction system and conditions were optimized in this rapid detection method.Results: The results of sensitivity analysis showed that the LAMP assay targeting the AP65 gene was 1000 times more sensitive than the nested PCR targeting the actin gene commonly used for detection of T. vaginalis, and the detecting limitation of the former was 10 trichomonad. Moreover, the amplification of the target gene AP65 by LAMP assay exhibited high specificity and the product was exclusively from T. vaginalis. The detection technique of LAMP did not exhibit cross-reactivity with the common pathogens of Trichinella spiralis, Toxoplasma gondii, Escherichia coli, Candida albicans, Staphylococcus aureus, Haemophilus.Conclusions: According to the present study, the LAMP assay with the target of AP65 gene, was suitable for the early diagnosis of T. vaginalis infections. Consequently, the LAMP assay was proposed by the current study as a point-of-care examination and an alternative molecular tool which exhibited the potential value in the treatment, control and prevention of trichomoniasis transmission and relevant complication.

Label-Free Rapid Detection of Pathogens with Antimicrobial Peptide Assisted Impedance Spectrometry

2015 ◽  
Vol 1793 ◽  
pp. 13-18 ◽  
Author(s):  
Keren Jiang ◽  
Hashem Etayash ◽  
Sarfuddin Azmi ◽  
Garima Thakur ◽  
Selvaraj Naicker ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Dynamic Range ◽  
Detection Method ◽  
Detection Methods ◽  
Handheld Devices ◽  
Gram Negative Bacteria ◽  
Label Free ◽  
Gram Positive ◽  
Gram Negative ◽  
Impedance Sensor

ABSTRACTAn urgent need exist for developing handheld devices for rapid, sensitive, and specific detection method for pathogens. Here we demonstrate a rapid detection method for Gram-positive and Gram-negative bacteria using an impedance sensor array functionalized with antimicrobial peptides (AMPs). This impedance sensor screens pathogens in real-time and has comparable sensitivity with current detection methods like polymerase chain reaction (PCR) and immunoassay. Functionalized electrodes in array selectively bind to the corresponding bacteria strains, resulting in variations in the impedance modulus. Impedance variation is used to detect incubated bacterial cell concentration with a resolution of 1 cell µL-1. The dynamic range of detection for both Gram-positive and Gram-negative bacteria is found to be 103-106cfu mL-1. Micropatterned electrodes modified with AMPs in an impedimetric array offer an excellent platform for rapid and selective detection of pathogens in contaminated water and food products.

Isothermal Target and Probe Amplification Assay for the Real-Time Rapid Detection of Staphylococcus aureus

2015 ◽  
Vol 78 (4) ◽  
pp. 723-727 ◽  
Author(s):  
HYEWON SHIN ◽  
MINHWAN KIM ◽  
EUNJU YOON ◽  
GYOUNGWON KANG ◽  
SEUNGYU KIM ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Rapid Detection ◽  
Food Poisoning ◽  
Detection Methods ◽  
Economic Losses ◽  
Fluorescence Detector ◽  
The Real ◽  
Robust Detection ◽  
Advantages And Disadvantages

Staphylococcus aureus, the species most commonly associated with staphylococcal food poisoning, is one of the most prevalent causes of foodborne disease in Korea and other parts of the world, with much damage inflicted to the health of individuals and economic losses estimated at $120 million. To reduce food poisoning outbreaks by implementing prevention methods, rapid detection of S. aureus in foods is essential. Various types of detection methods for S. aureus are available. Although each method has advantages and disadvantages, high levels of sensitivity and specificity are key aspects of a robust detection method. Here, we describe a novel real-time isothermal target and probe amplification (iTPA) method that allows the rapid and simultaneous amplification of target DNA (the S. aureus nuc gene) and a fluorescence resonance energy transfer–based signal probe under isothermal conditions at 61°C or detection of S. aureus in real time. The assay was able to specifically detect all 91 S. aureus strains tested without nonspecific detection of 51 non–S. aureus strains. The real-time iTPA assay detected S. aureus at an initial level of 101 CFU in overnight cultures of preenriched food samples (kiwi dressing, soybean milk, and custard cream). The advantage of this detection system is that it does not require a thermal cycler, reducing the cost of the real-time PCR and its footprint. Combined with a miniaturized fluorescence detector, this system can be developed into a simplified quantitative hand-held real-time device, which is often required. The iTPA assay was highly reliable and therefore may be used as a rapid and sensitive means of identifying S. aureus in foods.

scholarly journals Establishment and Application of Reverse Transcription Recombinase-Aided Amplification as A Rapid Detection Method for SARS-COV-2

2020 ◽  
Author(s):  
Lele Ai ◽  
Wei Liu ◽  
Fuqiang Ye ◽  
Chenxi Ding ◽  
Han Dai ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Detection Method ◽  
Detection Methods ◽  
Influenza B Virus ◽  
Influenza B ◽  
The Novel ◽  
Efficient Detection ◽  
Novel Coronavirus

Abstract Background: By the end of August 2020, >23 million cases and 800,000 deaths were attributed to SARS-CoV-2 in >200 countries. The improvement of simple, rapid, and efficient detection methods is of great significance for the early detection, timely isolation, and protection of susceptible populations. This study aimed to provide an alternative method for the rapid detection of viral nucleic acid.Methods: This study provided a rapid nucleic acid detection method mediated by recombinant enzyme based on the novel coronavirus (SARS-CoV-2). Primers and probes were designed based on the N gene sequence of coronavirus. The method was performed at 39 °C, the detection time was short (<20 min), and the detection limit was up to 101 copies/mL.Results: The primer-probe did not show any cross-reaction with adenovirus, Zika virus, influenza B virus, and chikungunya virus, with good specificity. A total of 106 clinical throat swab samples were compared by reverse transcription recombinase-aided amplification (RT-RAA) and commercial reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR); the results were identical.Conclusions: The novel coronavirus RT-RAA method established in this study had high sensitivity, strong specificity, simple operation, and fast detection speed, and hence, is suitable for the rapid detection of novel coronavirus under the current epidemic situation.

scholarly journals Establishment and Application of Reverse Transcription Recombinase-aided Amplification as a Rapid Detection Method for SARS-COV-2

2020 ◽  
Author(s):  
Lele Ai ◽  
Wei Liu ◽  
Fuqiang Ye ◽  
Chenxi Ding ◽  
Han Dai ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Detection Method ◽  
Detection Methods ◽  
Influenza B Virus ◽  
Influenza B ◽  
The Novel ◽  
Efficient Detection ◽  
Novel Coronavirus

Abstract Background: By the end of August 2020, >23 million cases and 800,000 deaths were attributed to SARS-CoV-2 in >200 countries. The improvement of simple, rapid, and efficient detection methods is of great significance for the early detection, timely isolation, and protection of susceptible populations. This study aimed to provide an alternative method for the rapid detection of viral nucleic acid.Methods: This study provided a rapid nucleic acid detection method mediated by recombinant enzyme based on the novel coronavirus (SARS-CoV-2). Primers and probes were designed based on the N gene sequence of coronavirus. The method was performed at 39 °C, the detection time was short (<20 min), and the detection limit was up to 101 copies/mL.Results: The primer-probe did not show any cross-reaction with adenovirus, Zika virus, influenza B virus, and chikungunya virus, with good specificity. A total of 106 clinical throat swab samples were compared by reverse transcription recombinase-aided amplification (RT-RAA) and commercial reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR); the results were identical.Conclusions: The novel coronavirus RT-RAA method established in this study had high sensitivity, strong specificity, simple operation, and fast detection speed, and hence, is suitable for the rapid detection of novel coronavirus under the current epidemic situation.

Assessment Criteria and Approaches for Rapid Detection Methods To Be Used in the Food Industry

2014 ◽  
Vol 77 (4) ◽  
pp. 670-690 ◽  
Author(s):  
MARTIN WIEDMANN ◽  
SIYUN WANG ◽  
LAURIE POST ◽  
KENDRA NIGHTINGALE
Keyword(s):  
Nucleic Acid ◽  
Foodborne Pathogens ◽  
Rapid Detection ◽  
Food Industry ◽  
Pathogen Detection ◽  
Assessment Criteria ◽  
Detection Methods ◽  
Advantages And Disadvantages ◽  
Specific Focus

The number of commercially available kits and methods for rapid detection of foodborne pathogens continues to increase at a considerable pace, and the diversity of methods and assay formats is reaching a point where it is very difficult even for experts to weigh the advantages and disadvantages of different methods and to decide which methods to choose for a certain testing need. Although a number of documents outline quantitative criteria that can be used to evaluate different detection methods (e.g., exclusivity and inclusivity), a diversity of criteria is typically used by industry to select specific methods that are used for pathogen detection. This article is intended to provide an overall outline of criteria that the food industry can use to evaluate new rapid detection methods, with a specific focus on nucleic acid–based detection methods.

Research Progress on Pulverized Coal Concentration Detection Device

2021 ◽  
Vol 15 ◽  
Author(s):  
Yun-Tao Wu ◽  
Tian-Hu Wang ◽  
Jin Hua ◽  
He-Yuan Sun
Keyword(s):  
High Efficiency ◽  
Coal Dust ◽  
Coal Industry ◽  
Pulverized Coal ◽  
Detection Methods ◽  
Research Progress ◽  
Future Research ◽  
Detection Scheme ◽  
Advantages And Disadvantages ◽  
Dust Detection

Background: Pulverized coal detection is an indispensable detection measure in the coal industry. The current detection devices can be divided into two types: invasive and non-invasive. The coal dust detection methods and devices based on acoustics, optics, and electricity have been extensively studied. In order to achieve a high-efficiency online detection scheme, improving the accuracy and stability of the detection means is the primary goal of the research. Objective: The general problems and characteristics of coal dust detection device design are summarized, as well as recent technological developments and the needs for online testing to predict future research trends. Methods: The current typical detection devices are classified according to the detection principle and whether they invade the target, analyzing its advantages and disadvantages according to the device performance and application scenarios. Results: It has a beneficial effect on the design of the pulverized coal concentration detection device. Conclusion: The paper summarizes and analyzes several representative coal concentration detection device patents in recent years. Then it points out advantages and main problems. On this basis, the main development direction of the coal dust concentration detection device in the future is discussed.

scholarly journals Comparative study of different detection method on the virus titers

2013 ◽  
Vol 2 (3) ◽  
pp. 6 ◽  
Author(s):  
Yunpeng Wang
Keyword(s):  
Cell Culture ◽  
Significant Role ◽  
Detection Method ◽  
Virus Detection ◽  
Culture Technique ◽  
Detection Methods ◽  
Research Progress ◽  
Experimental Animals ◽  
Cell Culture Technique ◽  
Plaque Detection

<p>The counting of the virions plays a significant role in the study of virology. The detection methods of the virus titers can be mainly divided into two kinds: that based on the experimental animals and that on the cell culture technique. The former can be applied to those viruses that possess the experimental animal model, and the latter, according to the pathological advances of the cells, can be further divided into direct observation of the pathological advances and the plaque detection. When it comes to those viruses, that either have no CPE symptom after having infected the cells, or have a complicated operation of the plaque detection, we can apply the direct immunoflorescence or indirect immunoflorescence to conduct detection. Real-time PCR performs an increasingly significant role in virus detection. As a foundation for the study of virology, the counting of the virions and its research progress are worthy of further attention.</p>

scholarly journals Research and Innovation of Rapid Test Method for Water Quality Analysis

2022 ◽  
Vol 2152 (1) ◽  
pp. 012038
Author(s):  
Zheng Lian ◽  
Changpeng Ai ◽  
Yonghua Zhou
Keyword(s):  
Water Quality ◽  
Rapid Detection ◽  
Rapid Test ◽  
Test Method ◽  
Quality Analysis ◽  
Detection Methods ◽  
Detection Accuracy ◽  
Water Quality Analysis ◽  
Advantages And Disadvantages ◽  
Research And Innovation

Abstract Water quality safety is closely related to people’s production and life, and it is necessary for human survival. In recent years, water pollution incidents occur frequently, which not only reminds us of environmental protection, but also exposes the lack of rapid detection ability of water quality analysis in China. At present, there are obvious shortcomings in the mainstream rapid detection methods of water quality analysis. Therefore, this paper puts forward the research and innovation of the rapid detection methods for water quality analysis. In this paper, the main methods of water quality analysis and detection in China are studied in depth, and the advantages and disadvantages of various methods are compared and analyzed. The results show that most of the current mainstream detection methods have the contradiction between detection cost and detection accuracy. In view of this situation, according to the actual needs of rapid detection of water quality analysis, combined with vacuum detection tube electronic colorimetry, this paper innovatively proposed a rapid detection method of water quality analysis based on vacuum detection tube electronic colorimetry. In this paper, the principle of this method is introduced in detail. Through the relevant comparative test experiments, it can be seen that in the detection experiment of pH value and nitrite concentration, the vacuum detection tube electron colorimetry method in this paper has obvious advantages over the traditional spectrophotometer. This paper analyzes that the innovation research of water quality analysis and detection is the innovation of the whole process and the whole system. Therefore, this paper puts forward the ideas and specific optimization and improvement measures for the management work, industry standards and work concept of water quality detection.

Sign in / Sign up

Export Citation Format

Share Document