Transfer of Campylobacter and Salmonella from Poultry Meat onto Poultry Preparation Surfaces

2017 ◽  
Vol 80 (5) ◽  
pp. 750-757 ◽  
Author(s):  
Amreeta Sarjit ◽  
Gary A. Dykes
Keyword(s):  
Thin Layer ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Food System ◽  
Chicken Meat ◽  
Poultry Meat ◽  
Transfer Rates ◽  
Thermophilic Campylobacter ◽  
Associated Surfaces ◽  
Foodborne Infection

ABSTRACT Thermophilic Campylobacter and Salmonella enterica are major causes of gastrointestinal foodborne infection. Survival of these pathogens on food-associated surfaces is a risk contributing to their spread through the food system. This study examined the transfer of two strains each of C. jejuni, C. coli, Salmonella Enteritidis, and Salmonella Typhimurium from chicken meat to a knife or scissors used on either a plastic or wooden cutting board. Each strain of Campylobacter and Salmonella at ∼108 CFU mL−1 was inoculated (5 mL) onto 25 g of chicken meat with skin and allowed to attach (for 10 min). The meat was then cut (20 times per implement) into 1-cm2 pieces with either a knife or scissors on either a plastic or wooden cutting board. The numbers of pathogens transferred from meat onto cutting implements and cutting board surfaces were enumerated. The surfaces were subsequently either rinsed with water or rinsed with water and wiped with a kitchen towel to mimic commonly used superficial cleaning practices for these implements, and the numbers of pathogens were enumerated again. The bacterial numbers for both pathogens were determined on thin-layer agar. The attachment of the Salmonella strains to chicken meat (∼7.0 to 7.8 log CFU cm−2) was higher than the attachment of the Campylobacter strains (∼4.6 to 6.6 log CFU cm−2). All four Salmonella strains transferred in higher numbers (∼1.9 to 6.3 log CFU cm−2) to all surfaces than did the Campylobacter strains (∼1.1 to 3.9 log CFU cm−2). The transfer rates of both pathogens from the chicken meat to all the surfaces examined varied substantially between ∼0 and 21.1%. The highest rate of transfer (∼21.1%) observed was for C. coli 2875 when transferred from the chicken meat to the scissors. Most cleaning treatments reduced the numbers of both pathogens (∼0.3 to 4.1 log CFU cm−2) transferred to all the surfaces. Our study gives insights into the risks associated with the transfer of Campylobacter and Salmonella from poultry to the surfaces used in poultry preparation.

scholarly journals Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia

2016 ◽  
Vol 95 (8) ◽  
pp. 1888-1893 ◽  
Author(s):  
T.Y. Thung ◽  
N.A. Mahyudin ◽  
D.F. Basri ◽  
C.W.J. Wan Mohamed Radzi ◽  
Y. Nakaguchi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Chicken Meat ◽  
Retail Markets

Prevalence of Salmonella Isolates and Antimicrobial Resistance in Poultry Meat from South Korea

2014 ◽  
Vol 77 (9) ◽  
pp. 1579-1582 ◽  
Author(s):  
RAN-HEE YOON ◽  
SE-YEOUN CHA ◽  
BAI WEI ◽  
JAE-HEE ROH ◽  
HYE-SUK SEO ◽  
...  
Keyword(s):  
South Korea ◽  
Foodborne Pathogens ◽  
Salmonella Enteritidis ◽  
Chicken Meat ◽  
Poultry Meat ◽  
Antibiotics Resistance ◽  
Shopping Malls ◽  
Internet Shopping ◽  
Traditional Markets ◽  
Meat Samples

Contamination of Salmonella was assessed in duck and chicken meat collected from supermarkets, traditional markets, internet shopping malls, and wholesale markets in Jeonlado, South Korea, in 2013. Salmonella contamination was found in 51.3% of duck meat samples and 3.7% of chicken meat samples. Salmonella contamination of duck meat samples differed by meat type, i.e., 69.8% of samples of whole ducks and 33.9% of samples of duck pieces. Six serotypes were identified from 64 Salmonella isolates in duck meat: Salmonella Typhimurium (37.5%), Salmonella Enteritidis (21.8%), Salmonella Stanley (3.1%), Salmonella Regent (1.6%), Salmonella Winterthur (3.1%), and Salmonella Westhampton (1.6%). All isolates were resistant to one or more antibiotics. Resistance to sulfisoxazole was most common (93.8% of isolates), followed by resistance to nalidixic acid (59.4%), ceftazidime (26.6%), and ampicillin (26.6%). To our knowledge, this study is the first to report Salmonella contamination in duck meat from Korea. Duck meat should be considered an important source of foodborne pathogens.

Salmonella enterica Serotypes Isolated from Imported Frozen Chicken Meat in the Canary Islands

2005 ◽  
Vol 68 (12) ◽  
pp. 2702-2706 ◽  
Author(s):  
T. HERNANDEZ ◽  
A. SIERRA ◽  
C. RODRIGUEZ-ALVAREZ ◽  
A. TORRES ◽  
M. P. AREVALO ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enterica ◽  
Salmonella Enteritidis ◽  
Phage Type ◽  
Country Of Origin ◽  
The United States ◽  
Chicken Meat ◽  
Breast Meat ◽  
Phage Types ◽  
Salmonella Virchow

To determine the prevalence of Salmonella enterica serotypes in imported frozen chicken meat, 406 samples (whole chicken, legs, and breast meat) were analyzed for Salmonella according to ISO6579 rules, serotypes were assigned, and phage typing was conducted for Salmonella serotypes Enteritidis, Typhimurium, and Heidelberg. The overall frequency of Salmonella isolation was 16.5%. By country of origin, the highest percentage of cases was found among the samples from France followed by samples from Brazil. The differences between legs and breast meat were significant. The most frequently isolated serotype of Salmonella was Enteritidis, followed by Salmonella Heidelberg, Salmonella Typhimurium, and Salmonella Virchow. By country of origin, we identified a large percentage of serotype Salmonella Enteritidis in the samples imported from Brazil. There was a greater diversity of serotypes isolated from the French samples, and Salmonella Enteritidis was not the dominant strain. In the samples from the United States, the only serotype isolated was Salmonella Kentucky, although a smaller number of samples was analyzed. The Salmonella Enteritidis phage type that prevailed in both France and Brazil was 4. Phage types 204c and 204 were identified for Salmonella Typhimurium, and phage types 8, 31, and 37 were identified for Salmonella Virchow.

scholarly journals Investigating the Meat Pathway as a Source of Human Nontyphoidal Salmonella Bloodstream Infections and Diarrhea in East Africa

2020 ◽  
Author(s):  
John A Crump ◽  
Kate M Thomas ◽  
Jackie Benschop ◽  
Matthew A Knox ◽  
David A Wilkinson ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
East Africa ◽  
Salmonella Enteritidis ◽  
Virulence Genes ◽  
Diarrheal Disease ◽  
Bloodstream Infections ◽  
Human Infection ◽  
Poultry Meat ◽  
Produce Water

Abstract Background Salmonella Enteritidis and Salmonella Typhimurium are major causes of bloodstream infection and diarrheal disease in East Africa. Sources of human infection, including the role of the meat pathway, are poorly understood. Methods We collected cattle, goat, and poultry meat pathway samples from December 2015 through August 2017 in Tanzania and isolated Salmonella using standard methods. Meat pathway isolates were compared with nontyphoidal serovars of Salmonella enterica (NTS) isolated from persons with bloodstream infections and diarrheal disease from 2007 through 2017 from Kenya by core genome multi-locus sequence typing (cgMLST). Isolates were characterized for antimicrobial resistance, virulence genes, and diversity. Results We isolated NTS from 164 meat pathway samples. Of 172 human NTS isolates, 90 (52.3%) from stool and 82 (47.7%) from blood, 53 (30.8%) were Salmonella Enteritidis sequence type (ST) 11 and 62 (36.0%) were Salmonella Typhimurium ST313. We identified cgMLST clusters within Salmonella Enteritidis ST11, Salmonella Heidelberg ST15, Salmonella Typhimurium ST19, and Salmonella II 42:r:- ST1208 that included both human and meat pathway isolates. Salmonella Typhimurium ST313 was isolated exclusively from human samples. Human and poultry isolates bore more antimicrobial resistance and virulence genes and were less diverse than isolates from other sources. Conclusions Our findings suggest that the meat pathway may be an important source of human infection with some clades of Salmonella Enteritidis ST11 in East Africa, but not of human infection by Salmonella Typhimurium ST313. Research is needed to systematically examine the contributions of other types of meat, animal products, produce, water, and the environment to nontyphoidal Salmonella disease in East Africa.

scholarly journals Mobile Colistin Resistance Gene mcr-1 Detected on an IncI2 Plasmid in Salmonella Typhimurium Sequence Type 19 from a Healthy Pig in South Korea

2021 ◽  
Vol 9 (2) ◽  
pp. 398
Author(s):  
Dong Chan Moon ◽  
Su-Jeong Kim ◽  
Abraham Fikru Mechesso ◽  
Hee Young Kang ◽  
Hyun-Ju Song ◽  
...  
Keyword(s):  
South Korea ◽  
Salmonella Typhimurium ◽  
Bacterial Infections ◽  
Salmonella Enteritidis ◽  
Sequence Type ◽  
Intestinal Epithelial ◽  
Colistin Resistance ◽  
Salmonella Gallinarum ◽  
Additional Resistance ◽  
Food Animals

Colistin is considered the last resort for the treatment of multi-drug resistant Gram-negative bacterial infections. We studied colistin resistance and the mcr-1 gene carriage in Salmonella isolates recovered from food animals in South Korea between 2010 and 2018. Colistin resistance was found in 277 isolates, predominantly in Salmonella Enteritidis (57.1%) and Salmonella Gallinarum (41.9%). However, the mcr-1 gene was identified in only one colistin-resistant Salmonella Typhimurium (MIC = 16 µg/mL) isolated from a healthy pig. The mcr-1 carrying isolate presented additional resistance to multiple antimicrobials. The strain belonged to sequence type (ST)19 and carried various virulence factor genes that are associated with adhesion and invasion of Salmonella into intestinal epithelial cells, as well as its survival in macrophages. The mcr-1 gene was identified on an IncI2 plasmid and it was also transferred to the E. coli J53 recipient strain. The mcr-1-carrying plasmid (pK18JST013) in this study was closely related to that previously reported in S. Indiana (pCFSA664-3) from chicken in China. This is the first report of mcr-1 carrying S. Typhimurium in South Korea. The finding indicates the importance of regular screening for the presence of the mcr-1 gene in S. Typhimurium in food animals to prevent the spread to humans.

scholarly journals Multiple Techniques for Size Determination of Generalized Modules for Membrane Antigens from Salmonella typhimurium and Salmonella enteritidis

ACS Omega ◽  
2017 ◽  
Vol 2 (11) ◽  
pp. 8282-8289 ◽  
Author(s):  
Gianluigi De Benedetto ◽  
Paola Cescutti ◽  
Carlo Giannelli ◽  
Roberto Rizzo ◽  
Francesca Micoli
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Size Determination ◽  
Membrane Antigens

scholarly journals Machine Learning Applied to Near-Infrared Spectra for Chicken Meat Classification

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Sylvio Barbon ◽  
Ana Paula Ayub da Costa Barbon ◽  
Rafael Gomes Mantovani ◽  
Douglas Fernandes Barbin
Keyword(s):  
Near Infrared ◽  
Prediction Models ◽  
Nir Spectroscopy ◽  
Quality Parameters ◽  
Chicken Meat ◽  
Poultry Meat ◽  
Spectral Information ◽  
Support Vector ◽  
Near Infrared Spectra ◽  
Meat Samples

Identification of chicken quality parameters is often inconsistent, time-consuming, and laborious. Near-infrared (NIR) spectroscopy has been used as a powerful tool for food quality assessment. However, the near-infrared (NIR) spectra comprise a large number of redundant information. Determining wavelengths relevance and selecting subsets for classification and prediction models are mandatory for the development of multispectral systems. A combination of both attribute and wavelength selection for NIR spectral information of chicken meat samples was investigated. Decision Trees and Decision Table predictors exploit these optimal wavelengths for classification tasks according to different quality grades of poultry meat. The proposed methodology was conducted with a support vector machine algorithm (SVM) to compare the precision of the proposed model. Experiments were performed on NIR spectral information (1050 wavelengths), colour (CIEL∗a∗b∗, chroma, and hue), water holding capacity (WHC), and pH of each sample analyzed. Results show that the best method was the REPTree based on 12 wavelengths, allowing for classification of poultry samples according to quality grades with 77.2% precision. The selected wavelengths could lead to potential simple multispectral acquisition devices.

Sign in / Sign up

Export Citation Format

Share Document