scholarly journals Tối ưu hóa phản ứng realtime PCR nhằm phát hiện Streptococcus agalactiae

2021 ◽  
Vol 16 (1) ◽  
pp. 34-46
Author(s):  
Nguyễn Thị Thanh Thảo ◽  
Nguyễn Thị Trúc Phương ◽  
Nguyễn Thị Trúc Anh ◽  
Lương Thị Mỹ Ngân
Keyword(s):  
Staphylococcus Aureus ◽  
Chlamydia Trachomatis ◽  
Streptococcus Agalactiae ◽  
Gardnerella Vaginalis ◽  
Realtime Pcr

Streptococcus agalactiae (GBS) là tác nhân truyền nhiễm hàng đầu gây nhiễm trùng huyết sơ sinh giai đoạn sớm. Tầm soát GBS và tiêm kháng sinh dự phòng ở phụ nữ thai sản có thể giúp ngăn chặn hữu hiệu tỷ lệ nhiễm GBS ở trẻ sơ sinh. Phương pháp truyền thống phát hiện và nuôi cấy GBS trên đĩa thạch máu rất tốn thời gian, công sức và độ nhạy thấp. Nghiên cứu này tiến hành tối ưu hóa phản ứng realtime PCR với cặp mồi và mẫu dò được thiết kế nhằm phát hiện gen đặc hiệu cfb của GBS. Các thí nghiệm tối ưu hóa được thực hiện trên chủng S. agalactiae ATCC 13813. Độ đặc hiệu của quy trình tối ưu được kiểm tra trên DNA của chủng Staphylococcus aureus ATCC 25923, Gardnerella vaginalis và Chlamydia trachomatis. Ngoài ra, quy trình tối ưu được thử nghiệm trên 30 mẫu dịch phết âm đạo-trực tràng của phụ nữ mang thai trong giai đoạn 35-37 tuần. Quy trình tối ưu đặc hiệu với chủng GBS, có độ nhạy 50 bản sao/phản ứng, độ chính xác 99.94%, và hiệu quả khuếch đại EA% = 94.5%. Trong số 30 mẫu thử nghiệm, 10 mẫu được phát hiện là có hiện diện của GBS, trong khi nuôi cấy truyền thống chỉ phát hiện 08 mẫu có GBS.

Antimicrobial Activity of Quercus infectoria Gall and Its Active Constituent, Gallic Acid, against Vaginal Pathogens

2019 ◽  
Author(s):  
Mozhgan Mehri Ardestani ◽  
Atousa Aliahmadi ◽  
Tayebeh Toliat ◽  
Abdolhossein Dalimi ◽  
Zohreh Momeni ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Gallic Acid ◽  
Streptococcus Agalactiae ◽  
Trichomonas Vaginalis ◽  
Antimicrobial Agents ◽  
Ethanolic Extract ◽  
Gardnerella Vaginalis ◽  
Active Constituent ◽  
Quercus Infectoria

Vaginal infections are one of the most common reasons a woman visits a gynecologist. The increased resistance to conventional antibiotics is one of the main reasons for searching and developing new antimicrobial agents, especially those of natural origin. In traditional Persian medicine, the gall of Quercus infectoria has been claimed to eliminate vagina and cervix from excessive discharge. So, the aim of the present study was to evaluate the antimicrobial activity of ethanolic extract of Quercus infectoria gall as well as its active constituent, gallic acid, against some vaginal pathogens. In this study, the ethanolic extract of Quercus infectoria gall was obtained by maceration and standardized based on amount of gallic acid. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of this extract as well as its active compound, gallic acid, were determined against Candida spp., Gardnerella vaginalis, Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, Trichomonas vaginalis and Lactobacillus acidophilus. The results demonstrated remarkable activity of ethanolic extract of Quercus infectoria gall against investigated pathogens with MIC and MBC in the range between 0.125 mg/ml and 16 mg/ml. The most inhibitory and bactericidal activity was observed on Streptococcus agalactiae and Staphylococcus aureus. The effects of gall dried ethanolic extract on Trichomonas vaginalis showed 100 % inhibition of the parasitic growth with concentration of 800 µg/ml after 24 h incubation. The antimicrobial and anti-trichomonas activity of extract was more than gallic acidIt seems that ethanolic extract of Quercus infectoria gall could inhibit the growth of vaginal pathogens. Further preclinical and clinical studies are required to confirm the efficacy of this natural extract in vaginitis.

scholarly journals Rapid Detection of Mycoplasma bovis, Staphylococcus aureus and Streptococcus agalactiae in Cattle Bulk Tank Milk in Cyprus and Relations with Somatic Cell Counts

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 841
Author(s):  
Maria Liapi ◽  
George Botsaris ◽  
Costas Arsenoglou ◽  
Nikolas Markantonis ◽  
Christodoulos Michael ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Somatic Cell ◽  
Real Time ◽  
Streptococcus Agalactiae ◽  
Mycoplasma Bovis ◽  
Bulk Tank Milk ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
Cell Counts ◽  
Bulk Tank

One hundred and seventy-seven (177) bulk tank milk samples were analyzed with a commercially available real-time polymerase chain reaction kit and 11 (6.21%), 41 (23.16%), and 58 (32.77%) tested positive for Mycoplasma bovis, Staphylococcus aureus, and Streptococcus agalactiae, respectively. Statistical analysis revealed a significant relationship between the presence of S. aureus and S. agalactiae. Enumeration of somatic cells was performed in the same samples by flow cytometry. The somatic cell counts were found higher in S. aureus and S. agalactiae positive samples. No association was found between M. bovis presence and somatic cells counts. Low internal assay control Ct values were found to be related with high somatic cell counts. Noticeably, this is the first report for the presence of M. bovis in Cyprus. Therefore, its presence was confirmed by bulk tank milk culture, conventional PCR, and next generation sequencing. Furthermore, M. bovis was typed with multilocus sequencing typing and was allocated to sequence type 29 (ST 29). Real-time PCR in bulk tank milk samples is a useful tool to detect mammary infections, especially for neglected pathogens such as M. bovis.

TRICHOMONAS VAGINALIS E ANORMALIDES CITOPATÓLOGICAS NOS EXAMES CITOPATOLÓGICOS REALIZADOS EM UM LABORATÓRIO CLÍNICO DA PONTIFÍCIA UNIVERSIDADE CATÓLICA DE GOIÁS - GO: ESTUDO DE PREVALÊNCIA

2020 ◽  
Vol 47 (1) ◽  
pp. 6104
Author(s):  
Andrea Alves Ribeiro ◽  
Sara Rosa Peixoto ◽  
Gabriel Henrique Martins de Almeida Araújo ◽  
Carolina Tonello dos Santos Pelá Alcanfor
Keyword(s):  
Herpes Simplex ◽  
Chlamydia Trachomatis ◽  
Odds Ratio ◽  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Candida Sp

O Trichomonas vaginalis é um protozoário que causa uma das principais infecções sexualmente transmissíveis. O objetivo foi estimar a prevalência de Trichomonas vaginalis em um Laboratório escola de Goiânia-GO. É um estudo retrospectivo utilizando as fichas de requisição dos exames citopatológicos realizados no Laboratório Clínico da Pontifícia Universidade Católica de Goiás (LC-PUCGO) entre janeiro de 2013 a dezembro de 2015. As variáveis foram categorizadas no programa Excel 2013 e tabulados no programa Epi Info™ versão 7, para avaliação da associação entre as variáveis foi realizado o cálculo do Odds Ratio (OR), considerando um intervalo de confiança (IC) de 95% e o valor de significância p< 0,05. Do total dos 4558 exames citopatológicos satisfatórios foi encontrada uma prevalência de Lactobacillus sp., de 46,97% (2141/4558) seguida por bacilos 24,62% (1122/4558), bacilos e cocos de 21,68% (988/4558), cocos de 3,86%(176/4558) e bacilos e lactobacilos de 2,87% (131/4558). Os agentes patogênicos encontrados foram Gardnerella vaginalis 79,6% (1141/1433), Candida sp. 17,7% (253/1433), Trichomonas vaginalis de 2,2% (32/1433), Herpes simplex de 0,4% (6/1433) e Chlamydia trachomatis de 0,1% (1/1433). Dentre os 4558 exames citopatológicos, 14,39% (656/4558) apresentaram anormalidades citológicas. As anormalidades encontradas foram ASC-US 2,57% (117/656), LSIL 1,78 % (81/656), ASC-H 3,52 % (160/656), HSIL 1,08% (48/656), AGC NEO 0,22% (10/656), Carcinoma invasor 0,02 % (1/656). Houve uma associação estatisticamente significante com a presença de Trichomonas vaginalis e o padrão anaeróbio OR 44,89 (IC 95 %: 10.5-271,8 p < 0,000). A detecção da manifestação por Trichomonas vaginalis é bastante sensível por meio do exame citopatológico. A presença de Trichomonas vaginalis, em um percentual relativamente baixo, indica a necessidade de identificação dos fatores de risco e aconselhamento em relação à proteção, transmissão e tratamento dessa infecção.

scholarly journals Vaginitis y cervicitis en planificación familiar

1991 ◽  
Vol 42 (3) ◽  
pp. 223-226
Author(s):  
Clara Inés Vargas ◽  
Betty Lucy Galindo ◽  
Jorge Enrique Martinez
Keyword(s):  
Chlamydia Trachomatis ◽  
Gardnerella Vaginalis ◽  
Candida Sp ◽  
Las Mujeres

Con el fin de determinar los patógenos vaginales y cervicales, y su relación con los métodos de planificación, estudiamos a una población de 300 mujeres que planificaban con anovulatorios o dispositivos intrauterinos y las comparamos con un grupo de 144 mujeres que utilizaban métodos naturales o de barrera para su control natal. Al analizar los resultados observamos que el patógeno más comúnmente aislado de la vagina de las pacientes de los diferentes grupos fue la Gardnerella vaginalis [38. 7% ). que la Candida sp no se presentó con mayor frecuencia en el grupo de mujeres que planificaban con anovulatorios [9%, control 11% ). y que la frecuencia de aislamiento de la Chlamydia trachomatis del cervix de las mujeres que planificaban fue en aumento a través del estudio [muestra 1: 4.6%; muestra 4: 20.9%), no así el grupo control [muestra 1: 11%; muestra 4 : 12%). [Rev Col Obstet Ginecol 1991; 42[3): 223-226).

scholarly journals Possibility of application of dodicin hydrochloride in prevention of mastitis in cows

2006 ◽  
Vol 60 (3-4) ◽  
pp. 207-221
Author(s):  
Svetlana Joksovic ◽  
Vitomir Cupic ◽  
Vera Katic
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Agalactiae ◽  
Control Group ◽  
Main Approach ◽  
Laboratory Conditions ◽  
Streptococcus Uberis ◽  
Holstein Friesian ◽  
Experimental Group ◽  
Outer Environment

The main approach to curbing mastitis is to prevent the entry of microorganisms from the outer environment into the mammary gland, which is achieved by the use of papilla disinfection following every time of milking. The objective of this work was to examine the antimicrobial activity of the disinfectant dodicin hydrochloride, in fact the preparation that contains this disinfectant (DESU? M), against bacteria, the most frequent causes of mastitis in cows. The efficacy of modified DESU? Mwas examined under laboratory conditions using the quantitative test against the following microorganisms: Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus uberis, under the conditions in the field. The udders of an experimental group of 20 cows of the Holstein-Friesian breed were immersed in a solution of the modified preparation DESU ? M following every time of milking over a period of three months. In the second group of 10 cows of the Holstein-Friesian breed, marked as the control group, no disinfection was applied following the milkings. The preparation DESU? Mexhibited satisfactory antimicrobial efficacy against the most frequent causes (Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus uberis) of mastitis in cows under laboratory conditions. With the application of the preparation DESU? M, following every time of milking over a period of three months, the number of somatic cells was reduced by almost one half in comparison with their number at the start of the experiment. No residue of the modified preparation DESU ? M were found in any sample of milk from the experimental group of cows.

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