Investigation of extraction method effect on yeast beta glucan production

Extraction of 2-Acetyl-1-Pyrroline (2AP) in Pandan Leaves (Pandanus Amaryllifolius Roxb.) Via Solvent Extraction Method: Effect of Solvent

Jurnal Teknologi ◽

10.11113/jt.v67.2735 ◽

2014 ◽

Vol 67 (2) ◽

Cited By ~ 1

Author(s):

Norzita Ngadi ◽

Noor Yahida Yahya

Keyword(s):

Solvent Extraction ◽

Extraction Method ◽

Extraction Process ◽

Natural Sources ◽

Effect Of Solvent ◽

Method Effect ◽

Solvent Extraction Method ◽

Pandanus Amaryllifolius

Pandan (Pandanus amaryllifolius Roxb.) leaves are widely used in Malaysia as a source of natural flavoring. The major compound contributing to the characteristic flavour of Pandan is 2-acetyl-1-pyrroline (2AP). As the consumer requirement for use of natural flavours, extraction of components from natural sources has been sought. In this study, solvent extraction of 2AP from Pandan leaves was performed. The effect of solvent used during extraction process (i.e. methanol, ethanol, propanol) towards the yield of 2AP was investigated. The presence of 2AP was determined using GCMS. The results obtained showed that ethanol was the best solvent to extract 2AP from Pandan leaves compared to methanol as higher 2AP peak arises from ethanol chromatogram. However there is no 2AP detected when propanol was used as solvent. It is believed that polarity of the solvent plays an important role in the extraction of 2AP.

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Effect of Strains and Extraction Methods on β-Glucan Production, Antioxidant Properties, and Ftir Spectra From Mushroom Fruiting Bodies of Schizophyllum Commune FR. in Thailand

10.20944/preprints202102.0294.v1 ◽

2021 ◽

Author(s):

Naruemon Mongkontanawat1 ◽

Duangrutai Thumrongchote

Keyword(s):

Antioxidant Activity ◽

Extraction Method ◽

Chemical Structure ◽

Antioxidant Properties ◽

Schizophyllum Commune ◽

Extraction Methods ◽

Hot Water ◽

Beta Glucan ◽

Nutrition Value ◽

Medicinal Properties

Schizophyllum commune Fr, a native mushroom of Thailand, has a high nutrition value and it classified as a mushroom with medicinal properties, which can neutralize the growth of many cancer cells. Thus, the aim of this research was studied effect of S. commune strain and the extraction method on the quantity and properties of β-glucan. The five S. commune Fr strains consisted of Chanthaburi, 85-022, 85-023, 85-031, and 85-043, which used in this research. The β-glucan extraction method compared two different extraction: hot water (M1) and hot alkali extraction (M2), with control (native-MR). It found that Chanthaburi strain has the highest in β-glucan content 49.20 + 0.35 % (w/w), and high potential antioxidant activity (79.14 +0.77 DPPH % and 50.92 + 0.48 ABTS %) (p < 0.05). The extraction methods had no effect on the yield of β-glucan, except antioxidant properties and chemical structure of extract substance. The extract substance from M2 has significant the highest potential antioxidant activity (80.22 + 0.51 drink mushroom juice in can by using 1-day-old MR and adjust pH more than 7 can increase antioxidant properties of product.

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Enhancement of β-Glucan Biological Activity Using a Modified Acid-Base Extraction Method from Saccharomyces cerevisiae

Molecules ◽

10.3390/molecules26082113 ◽

2021 ◽

Vol 26 (8) ◽

pp. 2113

Author(s):

Enas Mahmoud Amer ◽

Saber H. Saber ◽

Ahmad Abo Markeb ◽

Amal A. Elkhawaga ◽

Islam M. A. Mekhemer ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Cell Death ◽

Biological Activity ◽

Extraction Method ◽

Apoptotic Cell ◽

Dry Weight ◽

Future Research ◽

Resistant Bacteria ◽

Acid Base ◽

Beta Glucan

Beta glucan (β-glucan) has promising bioactive properties. Consequently, the use of β-glucan as a food additive is favored with the dual-purpose potential of increasing the fiber content of food products and enhancing their health properties. Our aim was to evaluate the biological activity of β-glucan (antimicrobial, antitoxic, immunostimulatory, and anticancer) extracted from Saccharomyces cerevisiae using a modified acid-base extraction method. The results demonstrated that a modified acid-base extraction method gives a higher biological efficacy of β-glucan than in the water extraction method. Using 0.5 mg dry weight of acid-base extracted β-glucan (AB extracted) not only succeeded in removing 100% of aflatoxins, but also had a promising antimicrobial activity against multidrug-resistant bacteria, fungi, and yeast, with minimum inhibitory concentrations (MIC) of 0.39 and 0.19 mg/mL in the case of resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, respectively. In addition, AB extract exhibited a positive immunomodulatory effect, mediated through the high induction of TNFα, IL-6, IFN-γ, and IL-2. Moreover, AB extract showed a greater anticancer effect against A549, MDA-MB-232, and HepG-2 cells compared to WI-38 cells, at high concentrations. By studying the cell death mechanism using flow-cytometry, AB extract was shown to induce apoptotic cell death at higher concentrations, as in the case of MDA-MB-231 and HePG-2 cells. In conclusion, the use of a modified AB for β-glucan from Saccharomyces cerevisiae exerted a promising antimicrobial, immunomodulatory efficacy, and anti-cancer potential. Future research should focus on evaluating β-glucan in various biological systems and elucidating the underlying mechanism of action.

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Evaluation of the dark field method for large association of spread DNA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081814 ◽

1978 ◽

Vol 36 (2) ◽

pp. 190-191

Author(s):

Douglas C. Barker

Keyword(s):

Electron Microscopy ◽

Molecular Weight ◽

Mitochondrial Dna ◽

Extraction Method ◽

Field Method ◽

Dark Field ◽

Kinetoplast Dna ◽

Field Electron ◽

Field Electron Microscopy ◽

Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

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Question Wording Effects in the Assessment of Global Self-Esteem

European Journal of Psychological Assessment ◽

10.1027//1015-5759.16.1.13 ◽

2000 ◽

Vol 16 (1) ◽

pp. 13-19 ◽

Cited By ~ 31

Author(s):

Martin Dunbar ◽

Graeme Ford ◽

Kate Hunt ◽

Geoff Der

Keyword(s):

Reading Ability ◽

Factor Model ◽

Self Esteem ◽

Reading Scores ◽

Poor Reading ◽

Method Effect ◽

Two Samples ◽

Positive Item ◽

Method Effects ◽

Best Fit

Summary: Marsh (1996) produced evidence that method effects associated with negatively worded items might be responsible for the results of earlier factor analytic studies that reported finding positive and negative self-esteem factors in the Rosenberg Global self-esteem scale ( Rosenberg, 1965 ). He analyzed data collected from children using a 7-item self-esteem measure. This report details attempts to replicate Marsh 's analysis in data collected from two samples of adults who completed the full 10-item Global Self-Esteem (GSE) scale. The results reported here are similar to those given by Marsh in so much as a correlated uniquenesses model produced a superior fit to the data than the simple one factor model (without correlated uniquenesses) or the often reported two factor (positive and negative self-esteem) model. However, whilst Marsh reported that the best fit was produced by allowing negative item uniquenesses to correlate with each other, the model that produced the best fit to these data was one that contained correlated positive item uniquenesses. Supporting his claim that differential responding to negative and positive self-esteem items reflects a method effect associated with reading ability, Marsh also showed that factors associated with negative and positive items were most distinct among children who had poor reading scores. We report a similar effect among a sample of older adults where the correlation between these factors was compared across two groups who were selected according to their scores on a test of verbal reasoning.

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Confound It!

European Journal of Psychological Assessment ◽

10.1027/1015-5759/a000459 ◽

2019 ◽

Vol 35 (6) ◽

pp. 855-867 ◽

Cited By ~ 2

Author(s):

John T. Kulas ◽

Rachael Klahr ◽

Lindsey Knights

Keyword(s):

Social Desirability ◽

Response Pattern ◽

Experimental Manipulation ◽

Scoring Method ◽

Method Effect ◽

Coding Method ◽

The Social ◽

Current Article ◽

Method Factors ◽

Psychological Inventory

Abstract. Many investigators have noted “reverse-coding” method factors when exploring response pattern structure with psychological inventory data. The current article probes for the existence of a confound in these investigations, whereby an item’s level of saturation with socially desirable content tends to covary with the item’s substantive scale keying. We first investigate its existence, demonstrating that 15 of 16 measures that have been previously implicated as exhibiting a reverse-scoring method effect can also be reasonably characterized as exhibiting a scoring key/social desirability confound. A second set of analyses targets the extent to which the confounding variable may confuse interpretation of factor analytic results and documents strong social desirability associations. The results suggest that assessment developers perhaps consider the social desirability scale value of indicators when constructing scale aggregates (and possibly scales when investigating inter-construct associations). Future investigations would ideally disentangle the confound via experimental manipulation.

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