XÁC ĐỊNH THÀNH PHẦN HÓA HỌC VÀ KHẢ NĂNG KHÁNG VI SINH VẬT CỦA CAO CHIẾT ETHANOL LY TRÍCH TỪ THÂN RỄ LOÀI THIÊN NIÊN KIỆN PI-E (HOMALOMENA PIERREANA)

2020 ◽  
Vol 39 (03) ◽  
Author(s):  
VĂN HỒNG THIỆN ◽  
LÊ BÍCH TRÂM ◽  
NGUYỄN THANH LAN ◽  
HỒ NGUYỄN HOÀNG YẾN ◽  
LƯU HỒNG TRƯỜNG ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis

Nghiên cứu này đã xác định được mẫu nghiên cứu thu tại Vườn quốc gia Phú Quốc là loài Homalomena pierreana. Thông qua kỹ thuật sắc ký ghép khối phổ, 10 hợp chất thuộc nhóm sesquiterpene có trong cao chiết ethanol của thân rễ loài H. pierreana đã được xác định. Ngoài ra, cao chiết ethanol từ mẫu nghiên cứu cũng cho thấy khả năng kháng lại 6 chủng vi khuẩn kiểm định là Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis và Salmonella typhimurium.

scholarly journals Polyurethane Microstructures-a Good or Bad in vitro Partner for the Isoflavone Genistein?

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Corina Danciu ◽  
Florin Borcan ◽  
Codruta Soica ◽  
Istvan Zupko ◽  
Erzsébet Csányi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Salmonella Enteritidis ◽  
Practical Application ◽  
Preliminary Study ◽  
Micro Structures

In recent years polyurethane microstructures (PM) have gained increasing attention in the pharmaceutical field due to the importance of their practical application. Since finding that such a formulation with genistein could improve its applications, we have conducted a preliminary study regarding the in vitro antiproliferative (MCF7, MDA-MB-231 and T47D) and antimicrobial ( Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis (D), Bacillus subtilis, B. cereus, and Candida albicans) activity in order to test whether polyurethane micro structuresre present a good option for further modulation of genistein's bioavailability. It was concluded that the polyurethane micro structures are a bad in vitro partner for the isoflavone genistein.

scholarly journals Actividad antibacteriana de extractos hexánico y etanólico de macroalgas marinas de la Bahía de Ancón, Lima – Perú

2020 ◽  
Vol 31 (2) ◽  
pp. e17829
Author(s):  
Ramón Muñoz A. ◽  
Selma Santome ◽  
Jorge León Q.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Salmonella Typhimurium ◽  
San Francisco ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc ◽  
Para Determinar

En el presente trabajo se evaluaron las actividades antimicrobianas de extractos etanólicos y hexánicos de nueve especies de macroalgas marinas recolectadas en la zona intermareal de la playa San Francisco, Ancón, de Lima, Perú. La evaluación se realizó in vitro frente a un panel de bacterias estándar Gram positivas (Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633 y Enterococcus faecalis ATCC 51922) y Gram negativas (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027 y Salmonella Typhimurium ATCC 14028). Los extractos etanólicos fueron de mayor rendimiento (67%) en comparación a los hexánicos (33%), en especial frente a los Gram positivos, siendo el extracto obtenido de Ulva enteromorpha var. intestinalis y U. nematoidea los más activos frente a S. aureus ATCC 6538 (74.1 y 78.2 % de inhibición, respectivamente). Los extractos etanólicos de Cladophora sp y U. nematoidea frente a B. subtilis ATCC 6633 presentaron inhibiciones entre moderada y baja (52.5 y 33%, respectivamente). Los extractos etanólicos de U. enteromorpha y Cladophora sp frente a S. Typhimurium presentaron solo 40% de inhibición. El extracto etanólico de Cladophora sp fue el único que presentó actividad antimicrobiana frente a las cinco cepas bacterianas y fue elegida para determinar la Concentración Mínima Inhibitoria (CMI), que resultó en 12.5 y 25 mg/ml frente a B. subtilis ATCC 6633 y S. aureus ATCC 6538, respectivamente. Se concluye que, la mayoría de las macroalgas estudiadas contienen compuestos inhibitorios, cuyos extractos etanólico y hexánico son capaces de inhibir el crecimiento de bacterias patógenas.

scholarly journals ANTIBAKTERIJSKO DJELOVANJE PROPOLISA NA ODABRANE REFERENTNE SOJEVE BAKTERIJA

2020 ◽  
Vol 20 (1-2) ◽  
Author(s):  
Bojan GOLIĆ́ ◽  
Vesna KALABÁ ◽  
Tanja ILIĆ́ ◽  
Dragana KALABÁ ◽  
Nina ZRNIĆ́
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis

Alkoholna tinktura propolisa je najčešće korišten proizvod od propolisa na tržištu za liječenje slabijih infekcija u usnoj šupljini, angine, nekih kožnih oboljenja i slično. Zbog toga što je propolis još uvijek neoficijalan lek u apotekama, ispitali smo njegovu antimikrobnu aktivnost primjenom disk-difuzionog testa na šest referentnih bakterijskih sojeva Salmonella Enteritidis WDCM 00030, Salmonella Typhimurium WDCM 00031, Listeria monocytogenes WDCM 00020, Staphylococcus aureus WDCM 00032, Escherichia coli WDCM 00013 i Pseudomonas aeruginosa WDCM 00024. Cilj ovoga rada je bio da se ispitaju antibakterijska svojstva alkoholnog rastvora propolisa na referentne bakterijske kulture, kao i da se odredi tip djelovanja. Na osnovu dobijenih rezultata, može se zaključiti da su bakterijski sojevi Salmonella Enteritidis WDCM 00030, Salmonella Typhimurium WDCM 00031, Listeria monocytogenes WDCM 00020, Staphylococcus aureus WDCM 00032 i Pseudomonas aeruginosa WDCM 00024, jako osjetljivi na djelovanje alkoholne tinkture propolisa.

KHẢ NĂNG GÂY ĐỘC TẾ BÀO UNG THƯ VÀ KHÁNG KHUẨN CỦA DỊCH CHIẾT GANODERMA LUCIDUM VÀ HUMPHREYA ENDERTII TỪ VƯỜN QUỐC GIA PHƯỚC BÌNH

2020 ◽  
Vol 39 (03) ◽  
Author(s):  
NGUYỄN THỊ DIỆU HẠNH ◽  
NGUYỄN NGỌC ẨN ◽  
LƯU VĂN LUÔNG ◽  
NGUYỄN CÔNG VÂN ◽  
HỒ NGUYỄN HOÀNG YẾN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Salmonella Typhimurium ◽  
Bacillus Cereus ◽  
Ganoderma Lucidum ◽  
Salmonella Enteritidis ◽  
Viet Nam

Các loài nấm Linh chi được sử dụng nhiều trong y học cổ truyền và việc xác định các hoạt tính sinh học của các loài nấm Linh chi mới là cần thiết cho sự phát triển các sản phẩm từ dược liệu. Năm 2016, nấm quế Linh chi, Humphreya endertii, thuộc họ Ganodermataceae, đã được phát hiện ở Vườn quốc gia Phước Bình, tỉnh Ninh Thuận, Việt Nam. Nghiên cứu này đã khảo sát khả năng gây độc tế bào ung thư phổi NCI H460 và tế bào ung thư gan HepG2 của dịch chiết hai loài nấm Linh chi Ganoderma lucidum và H. endertii có nguồn gốc từ Vườn quốc gia Phước Bình, IC50 tương ứng cho dịch chiết G. lucidum và H. endertii là 1,78 ± 0,35 và 2,25 ± 0,28 mg/ml trên tế bào NCI H460; và 4,53 ± 0,48 và 4,13 ± 1,05 mg/ml trên tế bào HepG2. Bên cạnh đó, dịch chiết hai loài nấm này cũng thể hiện khả năng kháng 5 chủng vi khuẩn gây bệnh Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enteritidis và Salmonella typhimurium; dịch chiết nấm G. lucidum còn thể hiện sự kìm hãm với Escherichia coli trong khi dịch chiết H. endertii thì không có hoạt tính này

scholarly journals Ενδοπληθυσμιακή και διαπληθυσμιακή μελέτη πτητικών συστατικών της ομάδας Thymus sect. Teucrioides jalas

2011 ◽  
Author(s):  
Δανάη Πιταροκοίλη
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Streptococcus Pneumoniae ◽  
Culex Pipiens ◽  
Salmonella Enteritidis ◽  
Candida Krusei ◽  
Post Hoc

Tα διάφορα είδη θυμαριού (Τhymus L.) ήταν γνωστά από την αρχαιότητα και χρησιμοποιούνταν ευρέως υπό διάφορες μορφές στην Αίγυπτο, την Ελλάδα και την Ρώμη. Σο γένος Τhymus L. (ομάδα Menthae, υποοικογένεια Nepetoideae), ένα από τα οκτώ σημαντικότερα γένη της οικογένειας των Labiatae, χωρίζεται σε οκτώ sectiones. Η sectio Teucrioides περλαμβάνει τα Th. leucospermus, Th. hartvigii, καθώς επίσης και το σύνολο των υποειδών του Th. teucrioides, τα οποία είναι από τα πιο διακριτά, αλλά ταυτόχρονα και από τα πλέον ποικιλόμορφα μεταξύ των ελληνικών θυμαριών.Σο θέμα της παρούσας διδακτορικής διατριβής είναι αφενός η ενδοπληθυσμιακή και διαπληθυσμιακή μελέτη των taxa του Thymus sect. Teucrioides Jalas, καθώς επίσης και η απομόνωση και ταυτοποίηση φυσικών βιοδραστικών ουσιών ενός εκπροσώπου της sectio, του υποείδους Thymus teucrioides subsp. candilicus.΢υνολικά συλλέχθησαν 22 πληθυσμοί, από 3 έως 6 άτομα αναλόγως της αφθονίας των ατόμων που απάρτιζαν τον πληθυσμό, ειδών και υποειδών και πιθανών υβριδίων του Thymus sectio Teucrioides από όλη την περιοχή εξάπλωσής της sectio Teucrioides στην Ελλάδα. Μετά από απόσταξη των αιθερίων ελαίων μελετήθηκε η χημική σύστασή τους με αέρια χρωματογραφία (GC-FID, GC-MS) και κατόπιν πραγματοποιήθηκε στατιστική ανάλυση των αποτελεσμάτων (CA, PCA, ANOVA, Post Hoc Tests - Bonferroni).Επιπλέον, το εκχύλισμα του υποείδους Thymus teucrioides subsp. candilicus επεξεργάστηκε με τεχνικές υγρής χρωματογραφίας και απέδωσε σαράντα ένα διαφορετικούς δευτερογενείς μεταβολίτες, η δομική ταυτοποίηση των οποίων, πραγματοποιήθηκε με βάση τα φασματοσκοπικά τους δεδομένα.Από τους σαράντα ένα δευτερογενείς μεταβολίτες, οι οκτώ αποτελούν νέα φυσικά προϊόντα. Από τους οκτώ αυτούς δευτερογενείς μεταβολίτες, οι επτά είναι πρενυλιωμένα σεσκιτερπένια και έχουν πρωτότυπους ανθρακικούς σκελετούς (10-16) και ο όγδοος ανήκει στην κατηγορία των μονοτερπενίων (6).Διενεργήθηκαν φαρμακολογικοί έλεγχοι σε αριθμό μεταβολιτών ως προς την κυτταροτοξική δράση έναντι έξι καρκινικών σειρών με αξιόλογα αποτελέσματα. Παράλληλα πραγματοποιήθηκαν βιοδοκιμές αντιμικροβιακής δράσης στους μεταβολίτες 10, 12 και 13, τα αιθέρια έλαια και τα φυτικά εκχυλίσματα των Thymus teucrioides subsp. candilicus και Th. leucospermus έναντι των βακτηριακών στελεχών Streptococcus pneumoniae, multi resistant Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Clostridium perfringens, Salmonella enteritidis, Micrococcus flavus, και των μυκήτων Candida albicans, Candida krusei και Aspergillus fumigatus. Σέλος, ελέγχθηκε η προνυμφοκτόνος και απωθητική δράση των ανωτέρω αιθερίων ελαίων και φυτικών εκχυλισμάτων εκπροσώπων της sectio Teucrioides στο δίπτερο υγειονομικής σημασίας Culex pipiens biotype molestus (κοινό κουνούπι), φορέα του ιού του Δυτικού Νείλου, με ενδιαφέροντα αποτελέσματα.

scholarly journals ANÁLISE DA COR E DA ATIVIDADE ANTIMICROBIANA DE MICROCÁPSULAS CONTENDO LACTOFERRINA BOVINA

2014 ◽  
Vol 32 (2) ◽  
Author(s):  
LUCIANA HELENA MAIA PORTE ◽  
MARIA HELENA MIGUEZ ROCHA LEÃO ◽  
ALEXANDRE PORTE
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Spray Drying ◽  
Enterococcus Faecium ◽  
Micrococcus Luteus ◽  
Rhodococcus Equi ◽  
Streptococcus Faecium

Lactoferrina bovina (bLF), proveniente do soro de leite, foimicroencapsulada pela técnica de spray drying. Microcápsulascontendo 20 % de bLF foram produzidas, utilizando-se comomaterial de parede dextrina: amido octenilsuccinato (OSA) emdiferentes proporções: 100:00, 75:25, 50:50, 25:75 e 0:100 %.Foram avaliadas a cor e a estabilidade de cor das microcápsulassob armazenamento em ambientes com diferentes umidadesrelativas e a atividade antimicrobiana da lactoferrina liberadadas microcápsulas. As microcápsulas apresentaram cor clara etenderam a escurecer sob armazenamento em ambiente com altaumidade relativa. Verifi cou-se atividade inibitória das microcápsulasde bLF produzidas para diferentes bactérias Gram positivas(Bacillus subtilis CCT 2576, Staphylococcus aureus CCT 2740,Micrococcus luteus CCT 2692, Enterococcus faecium CCT 5079,Streptococcus faecium ATCC 10541, Rhodococcus equi CCT0541), Gram negativas (Pseudomonas aeruginosa ATCC 13388,Salmonella choleraesius CCT 4296, Escherichia coli CCT 0547) elevedura (Candida albicans ATCC 10231). A concentração inibitóriamínima (MIC) das microcápsulas variou de acordo com o microorganismotestado (MIC entre 2,5-100 mg.mL-1). Com exceção deB. subtilis (MIC entre 50-100 mg.mL-1 para as microcápsulas), aconcentração de bLF contida nas microcápsulas necessária parainibir o crescimento dos micro-organismos foi menor do que a bLFnativa. Esses resultados sugerem efeito de potencialização daatividade antimicrobiana da bLF após o processamento por spraydrying.

Pulsed-Light Inactivation of Food-Related Microorganisms

1999 ◽  
Vol 65 (3) ◽  
pp. 1312-1315 ◽  
Author(s):  
N. J. Rowan ◽  
S. J. MacGregor ◽  
J. G. Anderson ◽  
R. A. Fouracre ◽  
L. McIlvaney ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Listeria Monocytogenes ◽  
High Intensity ◽  
Salmonella Enteritidis ◽  
Pulsed Light ◽  
Bacterial Cultures ◽  
Light Pulses ◽  
Yeast Extract Agar

ABSTRACT The effects of high-intensity pulsed-light emissions of high or low UV content on the survival of predetermined populations ofListeria monocytogenes, Escherichia coli,Salmonella enteritidis, Pseudomonas aeruginosa,Bacillus cereus, and Staphylococcus aureus were investigated. Bacterial cultures were seeded separately on the surface of tryptone soya-yeast extract agar and were reduced by up to 2 or 6 log10 orders with 200 light pulses (pulse duration, ∼100 ns) of low or high UV content, respectively (P < 0.001).

scholarly journals Spatial Structure and Antimicrobial Activity of Cyclopropane Derivative of Limonene

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300
Author(s):  
Daniyar Sadyrbekov ◽  
Timur Saliev ◽  
Yuri Gatilov ◽  
Ivan Kulakov ◽  
Roza Seidakhmetova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Spatial Structure ◽  
Bacterial Strains ◽  
Gram Negative ◽  
X Ray ◽  
Cyclopropane Derivative

A cyclopropane derivative of limonene, (1 S, 4 S, 6 R)-7,7-dichloro-4-[(1 S)-2,2-dichloro-1-methylcyclopropyl]-1-methylbicyclo [4.1.0] heptane (compound 2), was synthesized and its structure was determined by NMR and X-ray crystallographic methods. In addition, an antimicrobial activity of the compound against Gram-positive ( Staphylococcus aureus, Bacillus subtilis) and Gram-negative ( Escherichia coli, Pseudomonas aeruginosa) bacterial strains was also scrutinized.

scholarly journals Antimicrobial activity of soft and purified propolis extracts

Medicina ◽  
2008 ◽  
Vol 44 (12) ◽  
pp. 977 ◽  
Author(s):  
Alvydas Pavilonis ◽  
Algirdas Baranauskas ◽  
Ligita Puidokaitė ◽  
Žaneta Maželienė ◽  
Arūnas Savickas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Phenolic Compounds ◽  
Klebsiella Pneumoniae ◽  
Gram Negative ◽  
Propolis Extract

Objective. To evaluate the antimicrobial activity of soft and purified propolis extracts. Study object and methods. Antimicrobial activity of soft and purified propolis extracts was determined with reference cultures of Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 33499, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 12459, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 8035, and fungus Candida albicans ATCC 60193. Microbiological tests were performed under aseptic conditions. Minimum inhibitory concentration (MIC) – the highest dilution of preparation (the lowest concentration of preparation) that suppresses growth of reference microorganisms – was determined. Results. Concentration of phenolic compounds in soft propolis extract that possesses antimicrobial activity against gram-positive (Staphylococcus aureus, Enterococcus faecalis) and gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis) is 0.587±0.054 mg and 0.587±0.054–0.394±0.022 mg (P>0.05) and in purified propolis extract – 0.427±0.044 mg and 0.256±0.02 mg (P>0.05). Klebsiella pneumoniae is most resistant to soft propolis extract when the concentration of phenolic compounds is 1.119± 0.152 mg and to purified propolis extract when the concentration of phenolic compounds is 1.013±0.189 mg (P>0.05). Spore-forming Bacillus subtilis bacteria are more sensitive to soft and purified propolis extracts when the concentration of phenolic compounds is 0.134±0.002 mg and 0.075±0.025 mg, respectively, and Bacillus cereus – when the concentration is 0.394±0.022 mg and 0.256±0.02 mg (P>0.05). Sensitivity of fungus Candida albicans to soft and purified propolis extracts is the same as Bacillus subtilis. Encapsulated bacterium Klebsiella pneumoniae is most resistant to antimicrobial action of soft and purified propolis extracts as compared with gram-positive Staphylococcus aureus and Enterococcus faecalis bacteria (P<0.05), gram-negative Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis (P<0.05), sporeforming Bacillus subtilis and Bacillus cereus bacteria (P<0.05), and fungus Candida albicans (P<0.05). There is no statistically significant difference between antimicrobial effect of soft propolis extract and purified propolis extract on gram-positive bacteria, gram-negative bacteria, spore-forming bacteria, encapsulated bacteria, and Candida fungus. Conclusions. Soft and purified propolis extracts possess antimicrobial activity. They could be recommended as natural preservatives in the manufacture of pharmaceutical products.

scholarly journals Composición química del aceite esencial de Petroselinum crispum (Mill) Nyman ex A.W. Hill “perejil” y determinación de su actividad antibacteriana

2012 ◽  
Vol 15 (2) ◽  
pp. 78-83
Author(s):  
Reider Vivanco T ◽  
Enrique León S ◽  
Américo Castro L ◽  
Norma J Ramos C
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Staphylococcus Epidermidis ◽  
Petroselinum Crispum

El estudio tuvo como objetivo analizar la composición química del aceite esencial de las hojas de Petroselinum crispum (Mill) Nyman ex A.W. Hill “perejil” y determinar su actividad antibacteriana. El aceite esencial se obtuvo tratando 20 kg de hojas frescas con un equipo de destilación por arrastre con vapor de agua; obteniéndose cuatro mililitros y un rendimiento de 0,02 por ciento v/p. En el análisis cualitativo se analizó su solubilidad en solventes orgánicos; los componentes químicos se determinaron por cromatografía de Gases/ Espectrometría de Masas (CG/EM), lográndose la elucidación de los siguientes compuestos: 1R-α-pineno, β-pineno, β-felandreno, p, α-dimetil estireno, (E)-2-caren-4-ol, 1,3-benzodioxol, 4-metoxi-6-(2-propenil)-, y 1,3-benzodioxol,4,7-dimetoxy-5-(2-propenil)-. La determinación de la actividad antibacteriana in vitro, se realizó por el método de difusión en agar, demostrándose actividad significativa frente a Staphylococcus aureus ATCC 25933 y Staphylococcus epidermidis cepa clínica, a las concentraciones de 100 y 50 por ciento; y para Pseudomonas aeruginosa ATCC 2783 a la concentración de 100 por ciento, teniendo de poca a ninguna actividad frente a Bacillus subtilis y Escherichia coli. Los resultados obtenidos muestran que el aceite esencial de Petroselinum crispum (Mill) Nyman ex A.W. Hill “perejil” presenta actividad antibacteriana.

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