scholarly journals The female urinary microbiota in relation to the reproductive tract microbiota

Gigabyte ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Chen Chen ◽  
Lilan Hao ◽  
Weixia Wei ◽  
Fei Li ◽  
Liju Song ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Bacterial Communities ◽  
Reproductive Tract ◽  
Uterine Cavity ◽  
Detailed Comparison ◽  
Female Reproductive Tract ◽  
Reproductive Age ◽  
Rrna Gene ◽  
Urogenital System ◽  
Urinary Microbiota

Human urine is traditionally considered to be sterile, and whether the urine harbours distinct microbial communities has been a matter of debate. Potential links between female urine and reproductive tract microbial communities is currently not clear. Here, we collected urine samples from 147 Chinese women of reproductive age and explored the nature of colonization by 16S rRNA gene amplicon sequencing, quantitative real-time PCR, and live bacteria culture. To demonstrate the utility of this approach, the intra-individual Spearman’s correlation was used to explore the relationship between urine and multiple sites of the female reproductive tract. PERMANOVA was also performed to explore potential correlations between the lifestyle and various clinical factors and urinary bacterial communities. Our data demonstrated distinct bacterial communities in urine, indicative of a non-sterile environment. Streptococcus-dominated, Lactobacillus-dominated, and diverse type were the three most common urinary bacterial community types in the cohort. Detailed comparison of the urinary microbiota with multiple sites of the female reproductive tract microbiota demonstrated that the urinary microbiota were more similar to the microbiota in the cervix and uterine cavity than to those of the vagina in the same women. Our data demonstrate the potential connectivity among microbiota in the female urogenital system and provide insight and resources for exploring diseases of the urethra and genital tract.

scholarly journals The female urinary microbiota in relation to the reproductive tract microbiota

2019 ◽  
Author(s):  
Chen Chen ◽  
Lilan Hao ◽  
Weixia Wei ◽  
Fei Li ◽  
Liju Song ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Bacterial Communities ◽  
Reproductive Tract ◽  
Chinese Women ◽  
Uterine Cavity ◽  
Detailed Comparison ◽  
Reproductive Age ◽  
Rrna Gene ◽  
Urogenital System ◽  
Urinary Microbiota

AbstractHuman urine is traditionally considered to be sterile, and whether the urine harbours distinct microbial communities has been a matter of debate. The potential link between female urine and reproductive tract microbial communities is currently not clear.Here we collected the urine samples from 147 Chinese women of reproductive age, and explored the nature of colonization by 16S rRNA gene amplicon sequencing, real-time qPCR and live bacteria culture. To demonstrate utility intra-individual Spearman’s correlation was used to explore the relationship between urine and multi-sites of the reproductive tract. PERMANOVA was also performed to explore potential correlations between the lifestyle and various clinical factors and urinary bacterial communities. Our data demonstrated distinct bacterial communities in urine, indicative of a non-sterile environment. Types of diverse, Streptococcus-dominated, and Lactobacillus-dominated were the three most common types in the cohort. Detailed comparison of the urinary microbiota to the multi-sites of reproductive tract microbiota demonstrated the urinary microbiota was more similar to the microbiota in the cervix and uterine cavity instead of vagina in the same women.Our data demonstrates the potential connectivity of the microbiota in the female urogenital system and provided insight into the exploration of urethra and genital tract diseases.

scholarly journals The vagino-cervical microbiome as a woman’s life history

2019 ◽  
Author(s):  
Zhuye Jie ◽  
Chen Chen ◽  
Lilan Hao ◽  
Fei Li ◽  
Liu Tian ◽  
...  
Keyword(s):  
Sexual Behaviors ◽  
Reproductive Tract ◽  
Human Microbiome ◽  
Human Microbiome Project ◽  
Amplicon Sequencing ◽  
Female Reproductive Tract ◽  
Reproductive Age ◽  
Rrna Gene ◽  
Microbiome Composition ◽  
Major Factors

The gut microbiome has been the center of attention for human commensal microbiome studies. The vaginal microbiome is also densely populated with bacteria, viruses and fungi, and the presence of microorganisms beyond the cervix is increasingly reported in non-infectious conditions1–3. Due to the over 90% of human sequences in female reproductive tract samples3,4, metagenomic information has been very limited. 16S rRNA gene amplicon sequencing studies have identified community types in the vaginal microbiota, and observed its dynamic changes due to menstrual cycles and sexual behaviors in small cohorts5,6. Here we perform metagenomic shotgun sequencing on cervical samples from 516 women of reproductive age (more than 10-fold of the Human Microbiome Project (HMP)4), and dissect major factors, especially pregnancy and delivery histories and contraception methods on the microbiome composition. Features of other body sites, such as mood fluctuations and facial speckles could potentially be deduced from the vagino-cervical microbiome. Our results offer an unprecedented glimpse into the microbiota in the female reproductive tract and imply disease susceptibilities that may be relieved by behavioural changes.

scholarly journals Endometrial microbiota composition is associated with reproductive outcome in infertile patients

2021 ◽  
Author(s):  
Inmaculada Moreno ◽  
Iolanda Garcia-Grau ◽  
David Perez-Villaroya ◽  
Marta Gonzalez-Monfort ◽  
Mustafa Bahçeci ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Live Birth ◽  
Reproductive Tract ◽  
Treatment Strategies ◽  
16S Rrna Gene Sequencing ◽  
Female Reproductive Tract ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Reproductive Outcome ◽  
Infertile Patients

ABSTRACTBackgroundPrevious evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage, or no pregnancy.MethodsHere we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments.ResultsA dysbiotic endometrial microbiota profile composed of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Haemophilus, Klebsiella, Neisseria, Staphylococcus and Streptococcus was associated with unsuccessful outcomes. In contrast, Lactobacillus was consistently enriched in patients with live birth outcomes.ConclusionsOur findings indicate that endometrial microbiota composition before embryo transfer is a useful biomarker to predict reproductive outcome, offering an opportunity to further improve diagnosis and treatment strategies.

scholarly journals Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Microbiome ◽  
2022 ◽  
Vol 10 (1) ◽  
Author(s):  
Inmaculada Moreno ◽  
Iolanda Garcia-Grau ◽  
David Perez-Villaroya ◽  
Marta Gonzalez-Monfort ◽  
Mustafa Bahçeci ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Live Birth ◽  
Reproductive Tract ◽  
Treatment Strategies ◽  
16S Rrna Gene Sequencing ◽  
Female Reproductive Tract ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Reproductive Outcome ◽  
Infertile Patients

Abstract Background Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy. Methods Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments. Results A dysbiotic endometrial microbiota profile composed of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Haemophilus, Klebsiella, Neisseria, Staphylococcus and Streptococcus was associated with unsuccessful outcomes. In contrast, Lactobacillus was consistently enriched in patients with live birth outcomes. Conclusions Our findings indicate that endometrial microbiota composition before embryo transfer is a useful biomarker to predict reproductive outcome, offering an opportunity to further improve diagnosis and treatment strategies.

244 Fertility Outcomes Associated with Reproductive Tract Cytokines and Microbiota in Postpartum Beef Cows

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 128-129
Author(s):  
Rebecca K Poole ◽  
Taylor B Ault-Seay ◽  
Rebecca R Payton ◽  
Phillip R Myer ◽  
Andrea S Lear ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Transforming Growth Factor Beta ◽  
Bacterial Communities ◽  
Reproductive Tract ◽  
Transforming Growth Factor ◽  
Pearson Correlation ◽  
Transrectal Ultrasound ◽  
Beef Cows ◽  
Rrna Gene ◽  
Concentration Data

Abstract Local immune activity in the reproductive tract is crucial in the response to uterine diseases, normal reproductive functions, and establishing pregnancy. Few studies have evaluated the influence of the local immune environment of the reproductive tract on fertility outcomes. The objectives were to 1) evaluate reproductive cytokine concentrations in postpartum cows undergoing estrus synchronization followed by timed artificial insemination (TAI) and 2) correlate reproductive bacterial communities with cytokine concentrations. Angus cows (n = 20) were subjected to a 7-Day Co-Synch protocol with pre-synchronization beginning 21 days prior (d -21) to TAI (d 0). Uterine and vaginal flushes were collected on d -21 and -2. Pregnancy was determined by transrectal ultrasound on d 30. Bacterial community profiling and analyses were conducted targeting the V1 to V3 hypervariable regions of the 16S rRNA gene. Cytokine concentrations for interleukin (IL)-1b, IL-6, IL-10, and transforming growth factor beta (TGF-β) were determined by commercial ELISA kits. Concentration data were analyzed using PROC MIXED and correlations using Pearson correlation in SAS. No differences were detected in vaginal samples (P >0.05). No differences in IL-10 or IL-1b concentrations were detected in uterine samples (P >0.05). Uterine TGF-β concentrations were greater in resulting pregnant than non-pregnant cows (44.0 ± 13.4 pg/mL vs 14.7 ± 4.9 pg/mL; P = 0.05). Uterine TGF-β was negatively correlated with the relative abundance of genera Treponema (r = -0.668; P = 0.05) in resulting non-pregnant cows on d -21. Uterine IL-6 concentrations were greater in resulting non-pregnant than pregnant cows (198.7 ± 21.8 pg/mL vs 144.3 ± 16.1 pg/mL; P = 0.05). Uterine IL-6 and the relative abundance of genera Butyrivibrio were positively correlated (r = 0.742; P = 0.02) in resulting non-pregnant cows on d -21. These results suggest possible relationships between uterine bacterial communities and cytokines prior to TAI that may ultimately affect fertility outcomes in beef cattle.

Uterine microbiome—low biomass and high expectations†

2018 ◽  
Vol 101 (6) ◽  
pp. 1102-1114 ◽  
Author(s):  
Iolanda Garcia-Grau ◽  
Carlos Simon ◽  
Inmaculada Moreno
Keyword(s):  
Bacterial Communities ◽  
Sampling Method ◽  
Reproductive Tract ◽  
Female Reproductive Tract ◽  
High Expectations ◽  
Downstream Analysis ◽  
Uterine Diseases ◽  
Human Fetal Development ◽  
Infertile Patients

AbstractThe existence of different bacterial communities throughout the female reproductive tract has challenged the traditional view of human fetal development as a sterile event. There is still no consensus on what physiological microbiota exists in the upper reproductive tract of the vast majority of women who are not in periods of infection or pregnancy, and the role of bacteria that colonize the upper reproductive tract in uterine diseases or pregnancy outcomes is not well established. Despite published studies and advances in uterine microbiome sequencing, some study aspects—such as study design, sampling method, DNA extraction, sequencing methods, downstream analysis, and assignment of taxa—have not yet been improved and standardized. It is time to further investigate the uterine microbiome to increase our understanding of the female reproductive tract and to develop more personalized reproductive therapies, highlighting the potential importance of using microbiological assessment in infertile patients.

scholarly journals In ovo microbial communities: a potential mechanism for the initial acquisition of gut microbiota among oviparous birds and lizards

2018 ◽  
Vol 14 (7) ◽  
pp. 20180225 ◽  
Author(s):  
Brian K. Trevelline ◽  
Kirsty J. MacLeod ◽  
Sarah A. Knutie ◽  
Tracy Langkilde ◽  
Kevin D. Kohl
Keyword(s):  
Gut Microbiota ◽  
Microbial Communities ◽  
Bacterial Communities ◽  
Reproductive Tract ◽  
Egg Yolk ◽  
Fungal Communities ◽  
In Ovo ◽  
Physiological Processes ◽  
Initial Acquisition ◽  
Culture Independent

Vertebrate gut microbiota mediate critical physiological processes known to affect host fitness, but the mechanisms that expose wildlife to pioneer members of this important microbial community are not well understood. For example, oviparous vertebrates are thought to acquire gut microbiota through post-natal exposure to the external environment, but recent evidence from placental mammals suggests that the vertebrate reproductive tract harbours microbiota that may inoculate offspring in utero . These findings suggest that oviparous vertebrates may be capable of acquiring pioneer microbiota in ovo , but this phenomenon remains unexplored. To fill this knowledge gap, we used culture-independent inventories to determine if the eggs of wild birds and lizards harboured in ovo microbial communities. Our approach revealed distinct in ovo bacterial communities, but fungal communities were indistinguishable from controls. Further, lizard eggs from the same clutch had bacterial community structures that were more similar to each other than to unrelated individuals. These results suggest that oviparous vertebrates may acquire maternal microbiota in ovo , possibly through the inoculation of egg yolk prior to shelling. Therefore, this study may provide a first glimpse of a phenomenon with substantial implications for our understanding of the ecological and evolutionary factors shaping gut microbial communities.

scholarly journals Taxonomical and Functional Assessment of the Endometrial Microbiota in A Context of Recurrent Reproductive Failure: A Case Report

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 205 ◽  
Author(s):  
Iolanda Garcia-Grau ◽  
David Perez-Villaroya ◽  
Davide Bau ◽  
Marta Gonzalez-Monfort ◽  
Felipe Vilella ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Reproductive Tract ◽  
16S Rrna Gene Sequencing ◽  
Reproductive Failure ◽  
Female Reproductive Tract ◽  
Gardnerella Vaginalis ◽  
Rrna Gene ◽  
Microbial Profile ◽  
Genetic Features ◽  
Metagenome Sequencing

Investigation of the microbial community in the female reproductive tract has revealed that the replacement of a community dominated by Lactobacillus with pathogenic bacteria may be associated with implantation failure or early spontaneous abortion in patients undergoing assisted reproductive technology (ART) treatment. Herein we describe taxonomically and functionally the endometrial microbiome of an infertile patient with repeated reproductive failures (involving an ectopic pregnancy and two clinical miscarriages). The microbiological follow-up is presented over 18-month in which the microbiota was evaluated in six endometrial fluid samples. The microbial profile of 16S rRNA gene sequencing showed a persistent infection with Gardnerella and other bacterial taxa such as Atopobium and Bifidobacterium. In addition, taxonomic and functional analysis by whole metagenome sequencing in the endometrial fluid sample collected before one clinical miscarriage suggested the presence of multiple Gardnerella vaginalis clades with a greater abundance of clade 4, usually associated with metronidazole resistance. These results revealed a persistent G. vaginalis endometrial colonization presenting genetic features consistent with antimicrobial resistance, biofilm formation, and other virulence factors, which could be related to the reproductive failure observed.

scholarly journals Variation in Sodic Soil Bacterial Communities Associated with Different Alkali Vegetation Types

2021 ◽  
Vol 9 (8) ◽  
pp. 1673
Author(s):  
Andrea K. Borsodi ◽  
Márton Mucsi ◽  
Gergely Krett ◽  
Attila Szabó ◽  
Tamás Felföldi ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Relative Abundance ◽  
Bacterial Communities ◽  
Soil Microbial Communities ◽  
Rrna Gene ◽  
Vegetation Types ◽  
Metabolic Potential ◽  
Sodic Soils ◽  
Sodic Soil ◽  
Strain Collection

In this study, we examined the effect of salinity and alkalinity on the metabolic potential and taxonomic composition of microbiota inhabiting the sodic soils in different plant communities. The soil samples were collected in the Pannonian steppe (Hungary, Central Europe) under extreme dry and wet weather conditions. The metabolic profiles of microorganisms were analyzed using the MicroResp method, the bacterial diversity was assessed by cultivation and next-generation amplicon sequencing based on the 16S rRNA gene. Catabolic profiles of microbial communities varied primarily according to the alkali vegetation types. Most members of the strain collection were identified as plant associated and halophilic/alkaliphilic species of Micrococcus, Nesterenkonia, Nocardiopsis, Streptomyces (Actinobacteria) and Bacillus, Paenibacillus (Firmicutes) genera. Based on the pyrosequencing data, the relative abundance of the phyla Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes and Bacteroidetes also changed mainly with the sample types, indicating distinctions within the compositions of bacterial communities according to the sodic soil alkalinity-salinity gradient. The effect of weather extremes was the most pronounced in the relative abundance of the phyla Actinobacteria and Acidobacteria. The type of alkali vegetation caused greater shifts in both the diversity and activity of sodic soil microbial communities than the extreme aridity and moisture.

scholarly journals The Female Reproductive Tract Microbiome—Implications for Gynecologic Cancers and Personalized Medicine

2021 ◽  
Vol 11 (6) ◽  
pp. 546
Author(s):  
Anthony E. Rizzo ◽  
Jennifer C. Gordon ◽  
Alicia R. Berard ◽  
Adam D. Burgener ◽  
Stefanie Avril
Keyword(s):  
Personalized Medicine ◽  
Reproductive Tract ◽  
Uterine Cavity ◽  
Convincing Evidence ◽  
Female Reproductive Tract ◽  
Microbial Colonization ◽  
Future Research ◽  
Gynecologic Cancers ◽  
And Personalized Medicine ◽  
Generation Sequencing

The microbial colonization of the lower female reproductive tract has been extensively studied over the past few decades. In contrast, the upper female reproductive tract including the uterine cavity and peritoneum where the ovaries and fallopian tubes reside were traditionally assumed to be sterile under non-pathologic conditions. However, recent studies applying next-generation sequencing of the bacterial 16S ribosomal RNA gene have provided convincing evidence for the existence of an upper female reproductive tract microbiome. While the vaginal microbiome and its importance for reproductive health outcomes has been extensively studied, the microbiome of the upper female reproductive tract and its relevance for gynecologic cancers has been less studied and will be the focus of this article. This targeted review summarizes the pertinent literature on the female reproductive tract microbiome in gynecologic malignancies and its anticipated role in future research and clinical applications in personalized medicine.

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