scholarly journals Hubungan Angiogenesis dengan Gambaran Morfologi dan Klinis Kanker Payudara pada Kultur Jaringan

2021 ◽  
Vol 35 (2) ◽  
pp. 8-11
Author(s):  
Darwito ◽  
Sonar S Panigoro ◽  
Muchlis Ramli
Keyword(s):  
Cell Line ◽  
Cross Sectional ◽  
Grade Iii

Pendahuluan : Seperti tumor padat lainnya kanker payudara dapat tumbuh dan bermetastasis bila memperoleh oksigen dan nutrisi yang cukup. Tanpa adanya suplai darah yang menyediakan oksigen dan nutrisi maka tumor akan diam atau bahkan mati. Suplai darah bisa terjadi apabila sel tumor mampu membentuk pembuluh darah baru, yang disebut angiogenesis, yang merupakan faktor terpenting agar sel kanker itu dapat tumbuh dan melakukan metastasis. Metode : Penelitian ini menggunakan studi prospektif cross sectional dengan melakukan kultur jaringan kanker payudara sebanyak 14 kasus. Eksplan diambil dari spesimen hasil operasi dan ditanam dalam medium kultur 3 dimensi yang telah diberi endhotelial cell line. Aktifitas angiogenesis dinilai secara semikuantitatif invitro. Pengamatan aktifitas angiogenesis dilakukan setiap 24 jam dengan menilai migrasi sel endotel selama 96 jam. Pengamatan didokumentasikan dengan memakai skoring 0-4. Hasil : Berdasarkan evaluasi terhadap 14 kasus yang dilakukan kultur, didapatkan korelasi antara skor angiogenesis dengan TNM (tumor lymnode metastase), grading, dan tipe histopatologi. Kanker dengan grade III, tipe scirous mempunyai nilai skoring (+4), tanpa memandang TNM. Diskusi : Angiogenesis dapat terjadi melalui beberapa cara yaitu melaui proses tunas atau bukan tunas. Pembentukan angiogenesis meliputi pembentukan jaringan kapiler baru yang merupakan awal pembuluh darah, sedangkan yang bukan melalui tunas angiogenesis meliputi proses menjadi besar, pemisahan, dan penggabungan calon pembuluh darah. Proses bukan tunas memerlukan proliferasi sel endotel untuk membentuk dinding pembuluh darah. Pada penelitian ini tidak ada korelasi antara umur, status menopaus, dan status masa tumor dengan derajat angiogenesis. Hanya pada tipe histopatologi dan yang mempunyai korelasi dengan derajat angiogenesis dengan korelasi koefisien p=0,019 (

Perfil Epidemiológico de Pacientes Renais Crônicos em Tratamento Conservador em um Hospital Escola do Sul de Minas/ Epidemiological Profile of Chronic Renal Failure Patients on Conservative Treatment in a Hospital in Southern School of Minas

1970 ◽  
Vol 4 (4) ◽  
pp. 49-60
Author(s):  
Camila Dorilêo Negretti ◽  
Pablo Girardeli Mendonça Mesquita ◽  
Nilo César do Vale Baracho
Keyword(s):  
Diabetes Mellitus ◽  
Conservative Treatment ◽  
Cross Sectional Study ◽  
Age Group ◽  
Sectional Study ◽  
Cross Sectional ◽  
Control Programs ◽  
Keywords Epidemiology ◽  
Epidemiological Profile ◽  
Grade Iii

Objetivo: Determinar o perfil epidemiológico dos pacientes com Doença Renal Crônica em tratamento conservador no ambulatório do Hospital Escola de Itajubá. Materiais e Métodos: Estudo descritivo, de corte transversal, de abordagem quantitativa. Realizado com 171 pacientes atendidos em tratamento conservador no ambulatório de nefrologia no período de janeiro de 2012 a dezembro de 2013. A coleta objetivou caracterizar idade, sexo, raça, estado civil e procedência. Também foram avaliados dados do primeiro atendimento como: valores de ureia e creatinina, o grau de DRC, a etiologia e o número de retorno. Resultados: Dos doentes renais crônicos avaliados, 50,3% eram do sexo masculino, 88% da raça branca, 54,4% casados e a maioria (70,8%) possuía faixa etária prevalente >60 anos. A maioria dos atendidos, 98,5% pertence a microrregião de referência. Quanto a dados de primeira consulta, o diabetes mellitus foi a principal etiologia (38%). Os valores de ureia e creatinina acima da referência preconizada foi observado na maioria dos pacientes e o grau III de DRC em 31% dos casos. O número médio de retornos após a primeira consulta foi de três retornos em 25,13% dos pacientes. Conclusão: A pesquisa permitiu o conhecimento do perfil epidemiológico dos portadores de DRC em tratamento conservador. Os achados reforçam a necessidade de implementação de políticas voltadas para promoção e prevenção à saúde com divulgação de mais programas de controle para minimizar o surgimento de novos casos da DRC. Palavras-chave: Epidemiologia. Doença Renal Crônica. Tratamento. ABSTRACT.Objective: Determine the epidemiological profile of patients with Chronic Kidney Disease (CKD) on conservative treatment at the Hospital Escola de Itajubá. Materials and Methods: A retrospective, descriptive, cross-sectional study of quantitative approach. It was conducted with 171 patients who were treated in conservative treatment at the nephrology clinic from January 2012 to December 2013. The collection aimed to characterize age, sex, race, marital status and origin. We also evaluated data from the first service as: urea and creatinine values, the degree of CKD the etiology and the number of return. Results: Among the chronic renal patients evaluated, 50.3% were male, 88% Caucasian, 54.4% were married and the majority (70.8%) was the most prevalent age group> 60 years. Most of the patients 98.5% belong to micro region of reference. As the first appointment data, diabetes mellitus was the major cause (38%). The urea and creatinine values above the recommended reference was observed in the majority of patients and the DRC grade III in 31% of cases. The average number of returns after the fisrt visit was three return in 25.13% of patients. Conclusion: The research gave us the knowledge of the epidemiological profile of patients with CKD on dialysis. The findings reinforce the need to implement policies for health promotion and prevention with more disclosure of control programs to minimize the appearance of new cases of CKD. Keywords: Epidemiology. Chronic Kidney Disease.Treatment.

scholarly journals Clinical Profiles and Types of Senile Cataract Patients: Experience of 30 Cases in Bangladesh

2019 ◽  
Vol 6 (1) ◽  
pp. 38-41
Author(s):  
Shamima Sultana ◽  
AQM Omar Sharif ◽  
Inamur Rahman Choudhury ◽  
MM Ehsanul Haque ◽  
Wahida Begum
Keyword(s):  
Cross Sectional Study ◽  
Senile Cataract ◽  
Cross Sectional ◽  
Data Collection Sheet ◽  
Study Population ◽  
Computer Based ◽  
Clinical Profiles ◽  
Patients Experience ◽  
Cataract Patients ◽  
Grade Iii

Background: Senile cataract can be expressed with different clinical presentation. Objective: The purpose of the present study was to find out the clinical profiles of senile cataract patients. Methodology: This cross-sectional study was conducted at National Institute of Ophthalmology, Dhaka, Bangladesh from January 1999 to December 2000 for a period of two (02) years. Patients with senile cataract were selected for study. The details clinical profiles of the study population were recorded like types, grades of cataract and visual acuity. Data were collected on pre-designed data collection sheet, compiled and appropriate statistical analysis was done using computer based software. Result: A total number of 60 eyes of cataract patients were recruited for this study. The mean age with the standard deviation was 58.8±6.055 years. Majority of the patients were suffering from total cataract that is nuclear plus cortical plus posterior subcapsular variety which was 31(51.7%) eyes of cataract patients. Majority of the patients were grade III (amber) type of nuclear sclerosis which was 26(43.3%) eyes of cataract patients. Most of the patients were presented with PL which was 32(53.3%) eyes of cataract patients. Conclusion: In conclusion majority of the patients are suffering from total cataract with grade III (amber) type of nuclear sclerosis. Journal of Current and Advance Medical Research 2019;6(1):38-41

scholarly journals Prevalence of hypothermia in the first hour of life of premature infants weighing ≤ 1500g

2020 ◽  
Vol 41 (spe) ◽  
Author(s):  
Tamara Soares ◽  
Géssica Almeida Pedroza ◽  
Márcia Koja Breigeiron ◽  
Maria Luzia Chollopetz da Cunha
Keyword(s):  
Premature Infants ◽  
Neonatal Intensive Care ◽  
Cross Sectional Study ◽  
Descriptive Statistics ◽  
Sectional Study ◽  
Axillary Temperature ◽  
Cross Sectional ◽  
Clinical Complications ◽  
Computerized Records ◽  
Grade Iii

Abstract Objective: To analyze the prevalence of hypothermia in the first hour of life of preterm infants with birth weight 1,500 g or less. Method: A cross-sectional study performed in a Neonatal Intensive Care Unit. Data obtained from 359 computerized records of premature infants admitted between 2012 and 2016. Descriptive Statistics and Poisson Regression were used. Results: Premature infants (66.9%) presented hypothermia in the first hour of life, with axillary temperature of 36.2ºC (35.7-36.6), associated with: diagnosis of preeclampsia (p = 0.001), small for gestational age (p = 0.029), and the need for chest compression in the delivery room (p = 0.001). In cases of peri-intraventricular hemorrhage grade III (75%) and death (78.9%), there was a prevalence of premature infants with hypothermia in the first hour of life. Conclusion: Hypothermia in the first hour of life was prevalent in preter m infants, being associated with clinical complications. The prevention of hypothermia in the first hour of life is fundamental in the reduction of diseases related to prematurity.

scholarly journals Correlation between P-Wave Terminal Force V1 (PTFV1) from 12-Lead ECG and Left Ventricular Diastolic Dysfunction in Patients Diagnosed With Hypertension

2020 ◽  
Vol 6 (2) ◽  
pp. 97-103
Author(s):  
Bambang Arie Hidayat Dalimunthe ◽  
Nizam Akbar ◽  
Refli Hasan ◽  
Harris Hasan ◽  
Andika Sitepu ◽  
...  
Keyword(s):  
Diastolic Dysfunction ◽  
Systolic Dysfunction ◽  
Hypertensive Heart Disease ◽  
Left Ventricular Diastolic Dysfunction ◽  
Left Ventricular ◽  
P Wave ◽  
P Value ◽  
Cross Sectional ◽  
Ventricular Diastolic Dysfunction ◽  
Grade Iii

Background: Patients diagnosed with hypertension will deteriorate into hypertensive heart disease which is characterized by diastolic dysfunction first followed by systolic dysfunction later in the course of the disease. Diastolic dysfunction of the left ventricle causes an increase in LVEDP as well as in the dimension of the left atrium. P-Wave Terminal Force V1 (PTFV1) which is derived from 12 lead ECG could help diagnose diastolic dysfunction in centers where echocardiography is not available. The purpose of this study was to determine the correlation of PTFV1 on the 12-lead Electrocardiography with diastolic dysfunction in patients diagnosed with hypertension in the outpatient clinic of Cardiac Center Adam Malik General Hospital in Medan. Methods: This is a cross-sectional study conducted from March 2019 until August 2019. Patients with hypertension who met the inclusion criteria were examined electrocardiographically to obtain PTFV1 value. Then echocardiography examination was then performed to assess the grades of diastolic dysfunction and other parameters. Analysis of correlation between PTFV1 values and diastolic dysfunction was then conducted. Results: From the clinical characteristics, there is no difference regarding age, sex , and risk factorsbetween the three diastolic dysfunction groups, while echocardiography characteristic shows more reduced EF in grade III diastolic dysfunction (36.5±7.7). Significant differences in PTFV1 are found among diastolic dysfunction groups. Grade I diastolic dysfunction has PTFV1 value of 23.8 mm.ms, grade II diastolic dysfunction has PTFV1 value of 34.1 mm.ms, and grade III diastolic dysfunction has PTFV1 value of 52.1 mm.ms, Significance of  p value is <0.001. There is a strong correlation between PTFV1 and diastolic dysfunction grade (r = 0.63 (P <0.001)). Cut off point of PTFV1 > 29.8 mm.ms can discriminate patients who have increased LAP with a sensitivity of 84% and specificity of 71%. Conclusions: PTFV1 is a simple screening tool which is widely available and correlate well with left ventricular diastolic dysfunction in patients with hypertension, which makes it a good alternative tool especially in areas where echocardiography is not readily available.

scholarly journals The Role Activity of Complement, TNFα & IL12 in Pathogenesis Dengue Virus

2010 ◽  
Vol 1 (1) ◽  
pp. 44
Author(s):  
Soegeng Soegijanto ◽  
Dian Dwi Sary ◽  
Budi Setiawan ◽  
Atsushi Yamanaka
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Study Groups ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Complement Activity ◽  
Grade Iii

Dengue Virus infection is always found in some part of the world especially South East Asia including Indonesia. The pathogenesis of Dengue Virus infection is still controversial. The aim of this study is to analyze the role complement activity, TNFα & IL12 in Dengue Virus infection especially in pathogenesis of Dengue Virus infection. Cross sectional study had been done since February 2009 in Dr. Soetomo Hospital Surabaya. Blood Sera of Dengue Virus infection were collected from Dengue Fever, and Dengue Hemorrhagic Fever patient who had been care in Paediatric. Dengue patients and time schedule for taking blood sample for examination CH50, TNFα & IL12 as follow: on the first day on admission, the second day, the third day. Study groups of patients as follow: Dengue Fever, 36; Dengue Hemorrhagic Fever grade I, 37; Dengue Hemorrhagic Fever grade II, 10; Dengue Hemorrhagic Fever grade III, 18; Dengue Hemorrhagic Fever grade IV, 6. In this study found that the higher activity complement which lower level CH50 was more identified on Dengue Shock Syndrome and Dengue Hemorrhagic Fever grade III than Dengue Fever cases. A concept of our study was focusing on manifestation of vascular leakage, measurement of complement activity CH50, TNFα & IL12 and clinical manifestation Dengue Hemorrhagic Fever. The examination of TNFα & IL12 in our study supported the role the activity complement. The conclusion are measurement CH50, TNFα & IL12 can be used as a predictive factor of the degree of Dengue Virus infection

Syringomyelia Resolution Following Chiari Surgery: A Novel Scale for Communication and Research

Neurosurgery ◽  
2020 ◽  
Vol 88 (1) ◽  
pp. E60-E66
Author(s):  
Jacques Lara-Reyna ◽  
John Chae ◽  
Umberto Tosi ◽  
Mark M Souweidane ◽  
Rafael Uribe-Cardenas ◽  
...  
Keyword(s):  
Chiari Malformation ◽  
Cross Sectional Area ◽  
Percentage Change ◽  
Grading System ◽  
Sectional Area ◽  
Cross Sectional ◽  
Size Grade ◽  
Grade Ii ◽  
The Mean ◽  
Grade Iii

Abstract BACKGROUND The pathophysiological connection between Chiari malformation and syringomyelia is accepted. Debate remains, however, how can we best define changes in syringomyelia following surgery. OBJECTIVE To introduce a grading system focusing on syrinx reduction based on routinely and reproducible radiological information, and provide a suggestion of the application of this scale for prediction of patient's prognoses. METHODS Data from 48 patients with Chiari malformation and syringomyelia were compiled. We calculated syrinx cross-sectional area by approximating an ellipse in the largest axial plane. We compared the percentage of reduction or enlargement following surgery. The percentage change was grouped into four grades: Grade 0 = Increasing size, grade I ≤ 50% reduction, grade II = 50% to 90% reduction, grade III ≥ 90% reduction. RESULTS A total of 89.6% of patients had syrinx improvement after surgery. A total of 5 patients were grade 0, 14 were grade I, 20 patients were grade II, and 9 patients met criteria for grade III. The mean postoperative syrinx area was 24.1 mm2 (0-169 mm2) with a mean syrinx reduction of 62.7%. CONCLUSION Radiological improvement of syringomyelia can be mathematically defined and standardized to assist in communication in outcome-based trials. Radiological resolution is expected most patients.

A Cross Sectional Study of Chronic Liver Disease with Reference to Portal Hypertension and Endoscopic Finding at Down Town Hospital, Guwahati, Assam

2021 ◽  
Vol 8 (27) ◽  
pp. 2373-2380
Author(s):  
Manabendra Nayak ◽  
Ghodke Chinmoy Pradeep
Keyword(s):  
Portal Hypertension ◽  
Liver Disease ◽  
Chronic Liver Disease ◽  
Cross Sectional Study ◽  
Chronic Liver ◽  
Sectional Study ◽  
Cross Sectional ◽  
Town Hospital ◽  
Pugh Class ◽  
Grade Iii

BACKGROUND Cirrhosis is a progressive clinical condition associated with considerable mortality and morbidity. It leads to a wide spectrum of characteristic clinical manifestations, mainly attributable to hepatic insufficiency and portal hypertension.1 In cirrhosis, primary diagnostic test for evaluation of upper-gastro-intestinal bleeding (UGIB) is endoscopy.2 The present study attempts to find out different clinical patterns of the chronic liver disease (CLD) with portal hypertension along with the endoscopic profile of the patients. METHODS It was a cross sectional study conducted in Down Town Hospital, Guwahati, Assam. RESULTS A cross sectional study was conducted at Downtown Hospital, Guwahati, Assam on patients diagnosed with chronic liver disease from 01 January 2017 to 31 January 2018. Male predominance was observed in this study with 84 % males and 16 % females. Male to female ratio was 5.25 : 1. Maximum patients (58 %) were observed in the age group of 40 to 60 years followed by 30 % in the age group of 60 to 80 years. The average age was 53.6 years. Most common aetiological factor was alcohol (66 %) followed by non-alcoholic steatohepatitis (NASH) (16 %). Other aetiologies were hepatitis-B, hepatitis-C and cryptogenic. 88 % cases were recorded in model for end stage liver disease (MELD) score range of 10 to 29. Ascites was noted in 88 % cases, of which 20 % had grade-1 and grade-2, 48 % had grade-3. Splenomegaly was noted in 70 % cases. Child-Pugh class-C consisted of 26 % cases (13) with grade-III varices followed by 18 % cases (9) with grade-II varices while Child-Pugh Class-B had 24 % cases (12) and 8 % cases (4) with grade-III and grade-II varices respectively. Child-Pugh class-A had all the cases with grade-I varices. CONCLUSIONS Alcoholism was the leading cause for cirrhosis followed by hepatitis-B, hepatitis-C, NASH and cryptogenic. Class-C had maximum number of cases with grade-III varices followed by class-B. MELD score ranged between 10 - 29 in majority. Severe anaemia was noted in 10 % cases. Thrombocytopenia a non-invasive indicator of oesophageal varices was noted in 70 % with different grades of oesophageal varices. KEYWORDS Endoscopy, Portal Hypertension, Chronic Liver Disease

Mitochondrial alterations in human gastric carcinoma cell line

2007 ◽  
Vol 293 (2) ◽  
pp. C761-C771 ◽  
Author(s):  
Hyoung Kyu Kim ◽  
Won Sun Park ◽  
Sung Hyun Kang ◽  
Mohamad Warda ◽  
Nari Kim ◽  
...  
Keyword(s):  
Cell Line ◽  
Elongation Factor ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Human Gastric Cancer ◽  
Heat Shock Protein 60 ◽  
Ags Cells ◽  
Gastric Carcinoma Cell ◽  
Cross Sectional ◽  
Mitochondrial Inner Membrane

We compared mitochondrial function, morphology, and proteome in the rat normal gastric cell line RGM-1 and the human gastric cancer cell line AGS. Total numbers and cross-sectional sizes of mitochondria were smaller in AGS cells. Mitochondria in AGS cells were deformed and consumed less oxygen. Confocal microscopy indicated that the mitochondrial inner membrane potential was hyperpolarized and the mitochondrial Ca2+concentration was elevated in AGS cells. Interestingly, two-dimensional electrophoresis proteomics on the mitochondria-enriched fraction revealed high expression of four mitochondrial proteins in AGS cells: ubiquinol-cytochrome c reductase, mitochondrial short-chain enoyl-coenzyme A hydratase-1, heat shock protein 60, and mitochondria elongation factor Tu. The results provide clues as to the mechanism of the mitochondrial changes in cancer at the protein level and may serve as potential cancer biomarkers in mitochondria.

scholarly journals HUBUNGAN INDEX MASSA TUBUH DENGAN GRADING PADA KANKER PAYUDARA

Biomedika ◽  
2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Arif Nurhidayat Prawirohardjo ◽  
Widyanti Soewoto ◽  
Untung Alfianto
Keyword(s):  
Cross Sectional ◽  
Grade Ii ◽  
Grade Iii

Kanker payudara merupakan kanker paling umum pada wanita di seluruh dunia dan merupakan kanker paling banyak terjadi pada wanita. WHO telah merekomendasikan klasifikasi IMT termasuk derajat underweight atau overweight berhubungan dengan peningkatan risiko beberapa penyakit tidak menular. Differensiasi tingkat keganasan untuk kanker payudara, menggunakan kriteria WHO yaitu system grading Nottingham (juga disebut modifikasi Elston_Ellis dari sistem grading Scarff-Bloom-Richardson). Penelitian ini bertujuan untuk mengetahui hubungan Index Massa Tubuh dengan Grading pada kanker payudara. Penelitian ini merupakan studi observasional analitik dengan desain Cross Sectional untuk mempelajari hubungan Indeks Massa Tubuh dengan Grading kanker payudara. Karaktersitik berdasarkan umur minimum 28 tahun dan usia maksimum 64 tahun, berat badan pasien minimum 40 kg dan maksimum 75 kg. Tinggi badan pasien minimum 141 cm dan maksimum 163 cm,skor IMT minimum 17.22 dan maksimum 31.18. Berdasarkan IMT dalam kategori Underwight ada 2 orang (6,7%), IMT dalam kategori normowight ada 18 orang (60,0%), dan pasien dengan IMT dalam kategori overwight ada 10 orang (33.3%), dengan demikian responden mayoritas dengan hasil pengukuran IMT dalam kategori normowight. Derajat diferensiasi sel dalam kategori Grade I ada 2 orang (6,7%), derajat diferensiasi sel dalam kategori Grade II ada 8 orang (26,7%), dan derajat diferensiasi sel dalam kategori Grade III ada 20 orang (66.7%), dengan demikian responden mayoritas dengan hasil pengukuran derajatdiferensiasiseldalam kategori Grade III. Pasien dengan grading kanker payudara grade 1 dengan IMT underweight ada 1 orang dan normowight ada 1 orang, pada grading kanker payudara grade II yang normoweight ada 7 orang dan overweight ada 1 orang, dan pada grading kanker payudara grade III dengan IMT underweight ada 1 orang normoweight ada 10 orang dan overweight ada 9 orang. IMT overwight lebih berisiko terhadap tingginya grade pada grading kanker payudara, nilai koefisen korelasi sebesar 0,396 dengan nilai p=0,045 (p<0,05) yang berarti terdapat hubungan yang signifikan antara Index Masa Tubuh dengan Grading pada kanker payudara. Kata Kunci: Kanker Payudara, Index Masa Tubuh, Differensiasi Histopatologi

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