scholarly journals Antibacterial Activities of Pseudomonas orientalis APD 16 Isolate Sponge-Associated Aplysina sp. from Enggano Island Against Escherichia coli and Staphylococcus aureus

2020 ◽  
Vol 1 (2) ◽  
pp. 62-67
Author(s):  
Risky Hadi Wibowo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Sea Water ◽  
Structural Complexity ◽  
Antibacterial Activities ◽  
Morphological Characters ◽  
Filter Feeder ◽  
Gram Staining ◽  
And Staphylococcus Aureus

Sponge is one of the invertebrates from the Porifera phylum. Sponge bodies have structural complexity with different cell layers. The sponge has many pores (ostium) on the surface of their body as a filter feeder. Sponge is recognized as organisms that have the potential because they can produce metabolites. Secondary metabolites produced by sponges are the result of the association of sponges with bacteria. The sponge used in this study is the Aplysina sp. sponge collected from Enggano island, Bengkulu Province. Aplysina sp. sponge is known to contain metabolites with antibacterial, antifungal and cytotoxic activity on cancer cells. This study aims to identify of potential isolate associated with Aplysina sp. sponge collected from Enggano island. Isolation of bacteria from Aplysina sp. sponge using Sea Water Complete (SWC) media. The isolate was screened by antagonistic test, morphological characters, Gram-staining, biochemical test and molecular identification. Based on the antagonistic test, APD 16 isolate could inhibit Escherichia coli and Staphylococcus aureus in Vitro. APD 16 isolate was identified molecularly using of 16S rRNAgenes analysis and it genetically close with Pseudomonas orientalis.

Preparation, Characterization and Antibacterial Activity of Chitosan Schiffbases

2011 ◽  
Vol 287-290 ◽  
pp. 1947-1951 ◽  
Author(s):  
Jing Xian Xu ◽  
Ye Ting Lin ◽  
Qiang Lin ◽  
Wen Yuan ◽  
Xue Qiong Yin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Schiff Bases ◽  
Optical Density ◽  
Elemental Analysis ◽  
Antibacterial Activities ◽  
Cp Mas Nmr ◽  
And Staphylococcus Aureus ◽  
Better Than

Two Schiff bases of chitosan (CTS) were synthesized from 4-methoxylbenzylaldehyde (CH3O-CTS) and 4-methylbenzylaldehyde(CH3-CTS). The Schiff bases were characterized by FTIR, DSC-TGA, solid13C CP-Mas NMR, and elemental analysis. Antibacterial activities of the Schiff bases against Escherichia coli and Staphylococcus aureus were measured by the optical density method. The antibacterial activity of the Schiff bases is better than that of the original CTS. The IC50of CH3O-CTS and CH3-CTS againstEscherichia coliis respectively 40.3 ppm and 43 ppm, which being 38.5 ppm and 39.5 ppm against Staphylococcus aureus, lower than IC50of chitosan, being 59.5 ppm and 52 ppm againstEscherichia coliand Staphylococcus aureus, respectively.

scholarly journals Structure-activity relationships of 3-substituted-5,5- diphenylhydantoins as potential antiproliferative and antimicrobial agents

2011 ◽  
Vol 76 (12) ◽  
pp. 1597-1606 ◽  
Author(s):  
Nemanja Trisovic ◽  
Bojan Bozic ◽  
Ana Obradovic ◽  
Olgica Stefanovic ◽  
Snezana Markovic ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Human Colon ◽  
Antibacterial Activities ◽  
E Coli ◽  
Structure Activity Relationships ◽  
Structure Activity ◽  
Almost All

A series of twelve 3-substituted-5,5-diphenylhydantoins was synthesized, including some whose anticonvulsant activities have already been reported in the literature. Their antiproliferative activities against HCT-116 human colon carcinoma cells were evaluated to determine structure-activity relationships. Almost all of the compounds exhibited statistically significant antiproliferative effects at a concentration of 100 ?M, while the derivative bearing a benzyl group was active even at lower concentrations. Moreover, their in vitro antibacterial activities against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and clinical isolates of Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus were evaluated. Only the 3-iso-propyl and 3-benzyl derivatives showed weak antibacterial activities against the Gram-positive bacterium E. faecalis and the Gram-negative bacteria E. coli ATCC 25922 and E. coli.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DARI ISOLAT CENDAWAN ENDOFIT ASAL TUMBUHAN SENDUDUK (Melastoma malabathricum L.) TERHADAP Escherichia coli DAN Staphylococcus aureus

2018 ◽  
Vol 8 (2) ◽  
pp. 7-12
Author(s):  
Israwati Harahap ◽  
Vivin Paddillah Rahmi ◽  
Nofripa Herlina
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Zone Of Inhibition ◽  
Melastoma Malabathricum ◽  
Symbiotic Microorganism ◽  
And Staphylococcus Aureus ◽  
Test Result

Endophytic fungi is a symbiotic microorganism which live inside plant tissues and not harm to their host. Several genera from endophytic fungi known to produce secondary metabolite compounds like antibiotics, anticancer, antifungal, antiviral and antimalarial. This study aimed to investigate antibacterial activity of endophytic fungi from senduduk (Melastoma malabathricum L.) against Escherichia coli and Staphylococcus aureus. Based on the test result, 11 isolates endophytic fungi from senduduk were known to have antibacterial activities. Isolate code 23 have ability to inhibit the growth of E.coli with diameters zone of inhibition is 22 mm and eight isolate endophytic fungi have ability to inhibit the growth of S.aureus (showed by inhibition zone).

scholarly journals Synthesis, Structures, and Antibacterial Activities of Hydrazone Compounds Derived from 4-Dimethylaminobenzohydrazide

2021 ◽  
Vol 68 (3) ◽  
pp. 567-574
Author(s):  
Guo-Xu He ◽  
Ling-Wei Xue
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Nmr Spectra ◽  
Antibacterial Activities ◽  
13C Nmr Spectra ◽  
X Ray Diffraction ◽  
X Ray ◽  
Mtt Method ◽  
Antibacterial Material ◽  
And Staphylococcus Aureus

A series of three new hydrazone compounds derived from the condensation reactions of 4-dimethylaminobenzohydrazide with 4-dimethylaminobenzaldehyde, 2-chloro-5-nitrobenzaldehyde and 3-methoxybenzaldehyde, respectively, were prepared. The compounds were characterized by elemental analysis, infrared and UV-vis spectra, HRMS, 1H NMR and 13C NMR spectra, and single crystal X-ray diffraction. Crystals of the compounds are stabilized by hydrogen bonds. The compounds were assayed for antibacterial (Bacillus subtilis, Escherichia coli, Pseudomonas fluorescence and Staphylococcus aureus) and antifungal (Aspergillus niger and Candida albicans) activities by MTT method. The results indicated that compound 2 is an effective antibacterial material.

scholarly journals Salicylate blockade of granulocyte adherence and the inflammatory response to experimental peritonitis

Blood ◽  
1979 ◽  
Vol 53 (5) ◽  
pp. 1018-1022
Author(s):  
PJ Spagnuolo ◽  
JJ Ellner
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Inflammatory Response ◽  
Sodium Salicylate ◽  
Nylon Fiber ◽  
Intraperitoneal Infection ◽  
And Staphylococcus Aureus ◽  
Human And Mouse

Aspirin profoundly inhibited the in vitro augmentation of human and mouse granulocyte adherence to nylon fiber induced by the bacterial products Escherichia coli endotoxin and Staphylococcus aureus culture filtrate. Granulocytes obtained from normal volunteers during the 48 hr following ingestion of aspirin did not respond normally to endotoxin stimulation. Furthermore, pretreatment of mice with sodium salicylate prior to intraperitoneal infection with Streptococcus pneumoniae impaired granulocyte exudation and resulted in uncontrolled bacteremia and greater lethality of infection.

scholarly journals Evaluation preliminary phytochemistry and antimicrobial activity of extracts in Eugenia uniflora L. on Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 10 (13) ◽  
pp. e329101320579
Author(s):  
Alexandre Horácio Couto Bittencourt ◽  
Jaqueline Souza Machado ◽  
Mariana Garcia da Silva ◽  
Braz Antônio Pereira Cosenza
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Eugenia Uniflora ◽  
E Coli ◽  
And Staphylococcus Aureus

Devido ao aumento da resistência de patógenos humanos e animais aos medicamentos atuais, pesquisas por novos antimicrobianos têm sido incentivadas, principalmente por meio de plantas medicinais. Eugenia uniflora L., conhecida como pitangueira, é popularmente utilizada com diversas funções terapêuticas, justificando estudos nesta espécie. Assim, os objetivos deste estudo foram avaliar a atividade antibacteriana “in vitro” de extratos de folhas e cascas de Eugenia uniflora L. sobre Escherichia coli e Staphylococcus aureus. Para as etapas de triagem fitoquímica e microbiologia, foram utilizadas as metodologias usuais nessas áreas para obtenção dos extratos e avaliação da atividade antibacteriana com uso de controle positivo e negativo. Os resultados obtidos mostraram que no extrato das folhas foi verificada a presença de saponinas, flavonóides, alcalóides e taninos, enquanto na casca foram obtidos apenas saponinas e alcalóides. Os extratos da folha e da casca foram capazes de inibir o crescimento de Stapylococcus aureus apenas, promovendo halos de inibição de meio de 19,4 mm e 14,4 mm, respectivamente. As análises permitiram concluir que os extratos apresentam metabólitos secundários interessantes e de grande potencial antimicrobiano e que esses extratos foram capazes de inibir apenas as cepas de S. aureus, sendo resistentes às cepas de E. Coli.

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