scholarly journals Antifungal Activities of Methanol Extract from Syzygium chlorantha and Hopea spp. against Pycnoporus Sanguineus

2021 ◽  
Vol 4 (2) ◽  
pp. 88-94
Author(s):  
Menti Saysa Harmen ◽  
Sitti Fatimah Mhd. Ramle

Antifungal activities of 6 methanol extract obtain from bark, sapwood and heartwood of Syzygium chlorantha and Hopea spp. were evaluated against the white rot fungi Pycnoporus sanguineus using a medium in which homogenised hyphae were dispersed. It is observed that Hopea spp. has antifungal activity against Pycnoporus sanguineus compared to Syzygium chlorantha. Hopea spp. bark showed the highest antifungal activity against Pycnoporus sanguineus with reaction showed very clear inhibition zone. Meanwhile, Hopea spp. sapwood showed partially clear inhibition zone. On the other hand, there are no antifungal activities occurring at heartwood of Hopea spp. and all parts of Syzygium chlorantha. The positive control glycyrrhizic acid dipotassium salt (GADS) showed clear inhibition zone from 50 ?g to 1000 ?g. The results of the sapwood and bark of Hopea spp. suggest that these extract have great potential as a source of fungi stats.

BioResources ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. 2719-2731 ◽  
Author(s):  
Yi P. Teoh ◽  
Mashitah M. Don ◽  
Salmiah Ujang

Wood-decaying fungi present a serious threat to items made from rubberwood (Hevea brasiliensis). Though conventional chemical control has been a successful method for preserving wood against stain and decay fungi growth, the effects of these chemicals are of concern because they create problems for the environment and public health. Pycnoporus sanguineus (P. sanguineus), is a white-rot fungus that invades wood during its growth, storage, or use, causing decay or other property changes. It was considered in this work as a potential source of bioactive compounds and investigated for its natural antifungal activity using a minimum inhibitory concentration assay against wood-degrading fungi. It was found that media consisting of 10.0 g/L malt extract, yeast extract, dextrose, and maltose, respectively at pH 4.7±0.2 provided the highest biomass production by P. sanguineus. Results showed that the antifungal properties of methanol and water extract of P. sanguineus mycelia and supernatant ranged from MIC values of 0.1 to 5.0 µg/µL. 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP) was found to be the major component in the extract of this fungus, based on analysis using gas chromatography – mass spectrometry.


2016 ◽  
Vol 63 (2) ◽  
Author(s):  
Grzegorz Janusz ◽  
Justyna Sulej ◽  
Magdalena Jaszek ◽  
Monika Osińska-Jaroszuk

Three species of white rot fungi: Cerrena unicolor, Phlebia lindtneri and Pycnoporus sanguineus were cultured in two different media under five different lighting conditions: dark, white, red, blue, and green light. Laccase, cellobiose dehydrogenase, and protease activities were examined in the samples. Blue light efficiently boosted laccase synthesis in C. unicolor and P. sanguineus, whereas the highest activities (20 654 nkat/l) of P. lindtneri laccase were observed when this fungus was maintained in green light. On the contrary, the green light allowed obtaining the highest activities of cellobiose dehydrogenase of C. unicolor and P. lindtneri, while CDH of P. sanguineus seems to be dependent on white light. It is clearly visible that differences in protease activities are noticeable not only between the lights variants but also among the media used. However, high proteases activities are correlated with light variants inducing laccase in Lindeberg and Holm medium. Contrary to the cellulose-based medium, where they are weak in light variants that lead to high CDH activities.


Holzforschung ◽  
2016 ◽  
Vol 70 (9) ◽  
pp. 877-884 ◽  
Author(s):  
Jie Gao ◽  
Jong Sik Kim ◽  
Nasko Terziev ◽  
Geoffrey Daniel

Abstract Softwoods (SW, spruce and fir) and hardwoods (HW, ash and beech) were thermally modified by the thermo-vacuum (Termovuoto) process for 3–4 h in the temperature range 160–220°C (TMW160–220°C) and their fungal durability were examined in soil-block tests with two brown rot (BR, Postia placenta, Gloeophyllum trabeum) and two white rot (WR, Pycnoporus sanguineus, Phlebia radiata) fungi. SW-TMW160–220°C were exposed to P. placenta and P. sanguineus and HW-TMW190–220°C to all fungal species. Considerable improvement (durability class 1–3) in decay resistance was only achieved for SW- and HW-TMW220°C. Thermal modification (TM) below 200°C influenced decay resistance negatively in case of some fungal species applied for both SW and HW. Judged by the durability class, decay resistance was higher in HW- than in SW-TMW at high TM temperature. Behavior of TM differed significantly between ash (ring-porous HW) and beech (diffuse-porous HW). A comparison between results of soil- and agar-block tests on Termovouoto wood demonstrated that the influence of testing method in terms of assignment to durability classes is not significant.


2019 ◽  
Vol 2 (1) ◽  
pp. 1-2
Author(s):  
Ayah Rebhi Hilles ◽  
Syed Mahmood ◽  
Mohd Arifin Kaderi ◽  
Ridzwan Hashim ◽  
Tara Jalal ◽  
...  

Discovery and development of new drugs from marine and freshwater animal remain one of the most challenging areas in recent marine sciences field. Thus, the object of current study to examine the antifungal activity of Asian swamp eel (Monopterus albus) skin mucus. Eel skin mucus aqueous and methanol extracts were prepared with different extract concentrations from 0.49 to 1000 μg/mL against fungus pathogens i.e. Aspergillus niger and Microsporum gypseum. The antifungal assay conducted using well diffusion method. The results showed a dose dependent decrease the fungal growth, at 100µl/well, the inhibition zone of methanol extract against M. gypseum (25.7±0.75) mm, while the aqueous one was (23.3±0.16) mm Whereas eel skin mucus methanol and aqueous extracts showed lower inhibition zone against Aspergillus niger at the same concentration which was (11.1±0.59) mm and (9.0±0.15) mm respectively. The methanol extract showed the highest inhibitory activity against M. gypseum because M. gypseum infect the upper layers of the skin and eel skin mucus protect eels from infections. The results were statistically significant with p < 0.001. In conclusion, the present study carried out to reveal the antifungal activities of eel skin mucus which might be use as a source of antifungal agent.


Author(s):  
Florencia Guerra ◽  
Abdullah Adil Ansari ◽  
Rajini Kurup ◽  
Gomathinayagam Subramanian

Aims: Senna alata (Carrion crow bush), Senna bicapsularis (Money bush) and Pityrogramma calomelanos (Wild maran) are known for their value in traditional medicine. The study was an effort to identify, environment friendly antifungal compounds from these locally available plants against fungal pathogen Curvularia lunata. Study Design: An experiment based study done to identify antifungal property and phytochemical identification of C. lunata. Place and Duration of Study: Plants were collected from the coastal areas of Guyana and identified at the Biodiversity Center, University of Guyana, Guyana between January 2017- May 2017. Methodology: Phytochemical extraction was conducted using soxhlet and rotovap apparatus. Hexane, methanol and aqueous extracts of plants were analyzed for antifungal properties. Antifungal activity was tested using the poisoned food and well diffusion technique. Data analysis of the study was done using R-Studio Program for statistical computing and graphics. A Tukey test was done along with ANOVA and Boxplots. Results: Methanol extract of P. calomelanos showed maximum antifungal activity with an inhibition percentage of 60.3% at 400 µl, with poison food technique. With well diffusion method, P. calomelanos showed an inhibition zone of 54 mm at 500 µl. Saponins, steroids and glycosides were identified with methanol extract of P. calomelanos. Conclusion: The finding of this study indicates, the possible use of P. calomelanos extract as an antifungal agent against C. lunata and its potential to control fungal plant diseases.


BioResources ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. 606-614 ◽  
Author(s):  
Jing Wang ◽  
Jian Li ◽  
Shujun Li ◽  
Camille Freitag ◽  
J. J. Morrell

Three extractives from China-fir were obtained by a sequential extraction processes with hexane, ethyl acetate, and methanol. The components of the three extractives were analyzed: (1) The gas chromatography-mass spectrometry (GC-MS) analysis showed that in addition to the presence of cedrol, naphthalenes comprised a relatively large percentage of both the hexane extract (10.39%) and the ethyl acetate extract (9.43%). (2) Total phenolic contents analysis showed that phenols took up 6.66 % of the ethyl acetate extract and 22.8% of the methanol extract. All extracts, even with low concentrations, presented fair antifungal activities against two white-rot fungi, Trametes versicolor and Irpex lacteusand two brown-rot fungi, Postia placenta and Gloeophyllum trabeum. Cedrol and naphthalenes were partly responsible for the bioactivities. The synergistic effect of phenols and antifungal compounds also contributed to the wood decay resistance.


1994 ◽  
Vol 40 (9) ◽  
pp. 795-798 ◽  
Author(s):  
Mark L. Ferrey ◽  
William C. Koskinen ◽  
Robert A. Blanchette ◽  
Todd A. Burnes

White rot fungi were able to mineralize the aromatic ring carbon of alachlor to CO2. After 122 days, 14 and 12% of the alachlor that was initially present in malt extract cultures supplemented with a wood substrate was mineralized at room temperature by Ceriporiopsis subvermispora and Phlebia tremellosa, respectively. Although Phanerochaete chrysosporium mineralized alachlor at 25 °C, it did so more slowly than the other two white rot fungi. The brown rot fungus Fomitopsis pinicola did not mineralize alachlor.Key words: alachlor, mineralization, white rot fungi, pesticide.


2017 ◽  
Vol 9 (3) ◽  
pp. 297-306 ◽  
Author(s):  
R. Ali ◽  
A. Rahim ◽  
A. Islam

7-Hydroxy-3',4'-methylenedioxy- and 7-benzyloxy-3',4'-methylenedioxy flavanones have been synthesized starting from 2,4-dihydroxyacetophenone. Subsequently biocidal activities of the flavanones have been investigated along with their corresponding chalcones against some bacterial and fungal strains. 2'-Hydroxy-4'-benzyloxy-3,4-methylenedioxy chalcone (5) and its corresponding flavanone (7) showed good antibacterial and antifungal activities against some selected bacterial and fungal strains. On the other hand, 2',4'-dihydroxy-3,4-methylenedioxy chalcone (4) showed no antibacterial and antifungal activities while its corresponding flavanone (6) showed a little antibacterial activity only at higher concentration but did not show antifungal activity. The synthesized chalcones and flavanones have been characterized using UV-Vis, IR and 1H NMRspectral data together with elemental analysis.


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