scholarly journals Phytochemical and Antioxidant Potential of Four Traditional Malaysian Medicinal Plants

2017 ◽  
Vol 5 (1) ◽  
pp. 9-14
Author(s):  
Norfarizan-Hanoon N.A ◽  
Nurraihana H ◽  
Hasmah A ◽  
Wan Rosli W.I.
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Frap Assay ◽  
Total Antioxidant ◽  
Different Levels

Phytochemicals and antioxidants can be found at different levels in medicinal plants that are associated with the use in chronic diseases treatment. The objective of the present study was to investigate the phytochemical and antioxidant properties of Albizia myriophylla, Garcinia opaca var. dumosa, Oxalis barrelieri and Tacca cristata, traditional medicinal plants used in healthcare by aborigines or 'Orang Asli' from Kelantan, Malaysia. The total antioxidant capacity was estimated by ABTS (2,2’ azinobis-(3-ethybenzthiazoline-6-sulfonic acid)), DPPH (1,1-diphenyl-2-picrylhydrazyl radical) and FRAP assay. The results showed that the four selected plants had significant differences in their total phenolic content (ranging from 45.92 ± 2.01 to 613.37 ± 8.92 mg GAE/g dry weight), in their total flavonoid content (ranging from 6.03 ± 1.03 to 19.29 ± 2.90 mg CE/g dry weight), in their tannins (ranging from 28.23 ± 7.39 to 64.99 ± 7.27 mg TAE/g dry weight), in their alkaloids (ranging from 0.40 to 3.27%) and in their saponins (ranging from 4.31 to 13.15%). The TEAC values of the extracts ranged from 112.12 ± 3.16 to 237.20 ± 1.55 µmol Trolox/g dry weight, from 8.38 ± 2.09 to 219.36 ± 23.48 µmol Trolox/g dry weight and from 136.83 ± 5.24 to 220.93 ± 48.71 µmol Trolox/g dry weight, for ABTS, DPPH and FRAP respectively. TEAC values determined by ABTS, DPPH and FRAP were well correlated with phenolics, flavonoids and tannins contents. Garcinia opaca demonstrated the highest total phenolics and tannins while Oxalis barrelieri showed the highest total flavonoid and alkaloid compared to other plants studied. Garcinia opaca also had shown the highest antioxidant potential, followed by Oxalis barrelieri. This study had shown that Garcinia opaca var. dumosa and Oxalis barrelieri can be promoted as the source of phenolics antioxidant and radical scavengers.

scholarly journals Antioxidant, Total Phenolic and Flavonoid Content of Selected Nigerian Medicinal Plants

2015 ◽  
Vol 14 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Matthias Onyebuchi Agbo ◽  
Philip Felix Uzor ◽  
Uchenna Nneamaka Akazie Nneji ◽  
Chidozie Uzoma Eze Odurukwe ◽  
Uchenna Basilia Ogbatue ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

Plant phenolics and flavonoids play a great role in scavenging free radicals in the body and act as antioxidants. Thus their determination is sometimes nedded. Total antioxidant capacity, total phenolic and flavonoid contents of the extracts of 10 medicinal plants (Lochnera rosea, Allamanda cathartica, Asplenium platyneuron, Euphorbia prostrate, Baphia nitida, Crotolaria retusa, Zapoteca portoricensis, Platycerium bifurcatum, Mussaenda afzelii and Craterosiphon scandens) from 7 botanical families growing in the tropical rainforest of Nigeria were included in this study. The total antioxidant capacity of the extracts was assessed by using the phosphomolybdate method. The phenolic content was determined by using Folin-Ciocalteau assay, while the total flavonoid was determined by the aluminium chloride colorimetric assay. The results obtained showed that the total antioxidant capacity for all the extracts were in the range of 0.888 ± 0.75 to 0.938 ± 0.00 mg EAA/g. The results showed that E. prostrate, P. bifurcatum and A. platyneuron were found to be the richest source of phenolic (97.77 ± 0.77, 87.62 ± 1.22 and 82.33 ± 0.30 mg GAE/g) while B. nitida and M. afzelii had the least total phenolic content (11.67 ± 0.09 and11.18 ± 0.30 mg GAE/g). The highest total flavonoid content was revealed in P. bifurcatum (648.67 ± 12.3 mg QE) while M. afzelii also had the least total flavonoid content (3.67 ± 0.00 mg QE/g). The ratio of flavonoid to the phenolic in each extract was also determined to ascertain extracts that are rich in flavonoids.Dhaka Univ. J. Pharm. Sci. 14(1): 35-41, 2015 (June)

Antioxidant capacity of hydroalcoholic extracts of Grewia serrulata DC and Grewia nervosa (Lour.) Panigrahi

2018 ◽  
Vol 9 (1) ◽  
pp. 121
Author(s):  
Mentham Ramesh ◽  
Chandu Babu Rao
Keyword(s):  
Total Phenolic Content ◽  
Superoxide Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential

scholarly journals Spectrophotometric determination of sun protective factor (SPF) and antioxidant potential of different solvent extracts of graptophyllum pictum

2019 ◽  
Vol 10 (SPL1) ◽  
Author(s):  
Noor Diyana Ibrahim ◽  
Wan Nur Farihah Che Mustafa ◽  
Mahendran Sekar ◽  
Khor Poh Yen ◽  
Seow Lay Jing
Keyword(s):  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Uv Spectrophotometry ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Natural substances extracted from plants have been gaining attraction as protective agents due to their safety and responsible for multiple biological effects on skins. The present study was to determine the total phenolic and flavonoid contents, Sun Protection Factor (SPF), and antioxidant potential of different solvent extracts of Graptophyllum pictum leaves. The dried powdered leaves were extracted by the cold maceration method, using three different solvents, i.e., methanol, ethanol, and water. The extracted leaves was tested for antioxidant activity using of DPPH (1,1-diphenyl-2-picryl-hydrazyl). Total phenolic content was determined using the Follin-Ciocalteu and for total flavonoid content with used the aluminium chloride. The SPF of all extracts were analyzed by ultraviolet (UV) spectrophotometry. The total phenolic content is the highest in methanol extract (377.61±0.31 µg/ml), and total flavonoid content is the highest in ethanol extract (158.06 ± 0.18 µg/ml). For DPPH free radical scavenging activity, ethanolic extract showed the maximum percentage of inhibition, and for SPF, methanol extract had displayed the highest. The study recommends that methanol is a good solvent for the extraction of sun-protective constituents from G. pictum.

scholarly journals EVALUATION OF ANTIOXIDANT POTENTIAL AND QUANTITATIVE ESTIMATION OF PHENOLIC AND FLAVONOID CONTENT IN SOME SELECTED NEPALESE MEDICINAL PLANTS

2019 ◽  
pp. 124-128
Author(s):  
RUPAK KHAREL ◽  
KHAGA RAJ SHARMA
Keyword(s):  
Medicinal Plants ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Melia Azedarach ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Methanolic Extracts ◽  
Ic50 Value ◽  
Phenolic And Flavonoid

Objective: The aim of this study is to evaluate the antioxidant potential, determination of total phenolic and flavonoid content in nine selected medicinal plants Spondias pinnata, Melia azedarach, Ageratina adenophora, Urtica dioica, Curcuma longa, Bauhinia variegata, Elaeocarpus angustifolius Blume, Achyranthes aspera, and Psidium guajava from Kavre district of Nepal using in vitro studies. Methods: Methanolic plant extracts were prepared by cold percolation method. The methanol extract of nine medicinal plants collected from Kavre district of Nepal, was screened for assessing bioactive phytoconstituents followed by antioxidant property, total phenolic, and flavonoid content. Different plants collected were powdered and extracted with methanol, concentrated by a rotatory evaporator and analyzed for the presence of phytochemicals. The antioxidant potential of the plant extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Results: The phytochemical analysis of methanolic extracts of all nine medicinal plants displayed the presence of various secondary metabolites such as alkaloids, flavonoids, polyphenols, saponins, and quinones. The extract of S. pinnata showed the highest percentage of radical scavenging activity up to 87.94±1.88 with 50% inhibitory concentration (IC50) 17.51±1.27 μg/mL, followed by B. variegata, 80.63±1.06 with IC50 value 26.55±2.61 μg/mL. The standard, ascorbic acid has IC50 value of 20.13±1.17 μg/mL. Further, the ethyl acetate fraction of S. pinnata showed the maximum percentage of radical scavenging (85.92±1.37) with IC50 value of 46.95±1.17 μg/mL. Moreover, S. pinnata displayed the highest total phenolic content (TPC) 48.26±1.23 mg GAE/g (milligram gallic acid equivalent per gram) extract while the highest flavonoid content was displayed by Melia azedarach 41.07±1.53 mg QE/g (milligram quercetin equivalent per gram) extract measured by the Folin–Ciocalteu reagent method and aluminum chloride colorimetric method. Conclusions: The preliminary results of this study have put forward the extract of S. pinnata showed the highest percentage of radical scavenging activity and S. pinnata displayed the highest TPC while the highest flavonoid content was displayed by Melia azedarach methanolic extracts although the further studies are needed to assess its mechanism of action.

scholarly journals Optimization of Uruset Apple Vinegar Production Using Response Surface Methodology for the Enhanced Extraction of Bioactive Substances

Foods ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 107 ◽  
Author(s):  
Seydi Yıkmış
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Process Conditions ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Bioactive Substances ◽  
Total Antioxidant

In this study, the aim is to produce non-thermal vinegar by using red Uruset apples, which have high bioavailability among apple varieties. For this purpose, Uruset apple vinegar was produced and ultrasound at different times (2, 4, 6, 8 and 10 min) and different amplitudes (40%, 50%, 60%, 70%, and 80%); in addition, a 26 kHz frequency was applied to the samples. Total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (1,1-diphenyl- 2-picrylhydrazyl (DPPH) and cupric reducing antioxidant capacity (CUPRAC)), and color values were evaluated for the optimization of process conditions. At the same time, the differences between commercial apple vinegar (CV), pasteurized Uruset apple vinegar (PV), and a control (C) of untreated apple vinegar were investigated. Ultrasound treatment of Uruset apple vinegar was more successful for the enrichment of bioactive substances than the other samples. At the end of the study, the maximal optimization values for Uruset apple vinegar were 7.4 min and 62.2 amplitude. At the end of optimization, CUPRAC (0.69 mg TEAC/mL), DPPH (0.49 mg TEAC/mL), total flavonoid content (46.95 mg CE/L), and total phenolic content (124.25 mg GAE/L) were determined. As a result, ultrasound technology was successfully used for Uruset apple vinegar production.

scholarly journals Antioxidant properties, total phenolic and total flavonoid content of the Slovak white wines – welschriesling and chardonnay

10.5219/730 ◽  
2017 ◽  
Vol 11 (1) ◽  
Author(s):  
Július Árvay ◽  
Daniel Bajčan ◽  
Alena Vollmannová ◽  
Judita Bystrická ◽  
Pavol Trebichalský ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
White Wines

scholarly journals Antioxidative properties and proximate analysis of spent coffee ground (SCG) extracted using ultrasonic-methanol assisted technique as a potential functional food ingredient

Food Research ◽  
2020 ◽  
Vol 4 (3) ◽  
pp. 636-644
Author(s):  
M.K. Zainol ◽  
I. Mohd Subri ◽  
Zamri Amir Izzwan ◽  
Zamzahaila Mohd Zin ◽  
Fisal Ahmad ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Functional Food ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Spent Coffee Ground ◽  
Coffee Ground

Spent coffee ground (SCG) produced in tons by restaurants and cafeterias and domestic consumers is a potentially good source of natural antioxidants because it contains substantial amounts of bioactive compounds. The purpose of this study was to identify the physicochemical and antioxidant properties of three different types of spent coffee ground (SCG), namely Robusta, Arabica and Liberica extracted using ultra-sonicmethanol assisted technique. DPPH, FTC, TBA, total phenolic content (TPC) and total flavonoid content (TFC) were used to measure the antioxidant properties. Robusta SCG exhibited the highest DPPH inhibition 41.63±0.04%), FTC (60.42±0.03%) and TBA analysis (73.09±0.08%). The total phenolic compounds in the samples varied widely ranging from 18.94±0.06 to 26.23±0.86 mg GAE/g sample, with Robusta SCG showing the highest value among the three, while Arabica SCG depicted the highest amount total flavonoid content (47.62±0.05 to 56.20±0.08). A strong correlation between antioxidant activity and total phenolic content were observed in this study. Compared to Arabica and Liberica SCGs expended, Robusta SCG demonstrated a stronger beneficial effect against lipid peroxidation. This study reveals that SCGs can be regarded as a new useful source of natural antioxidant with a view to increasing the use of antioxidant synthetics by using the ingredient of agro-industrial residues in food production especially ingredients for functional food.

scholarly journals Ramification of pH in pectinase-assisted extraction on the antioxidant capacity of Arabica spent coffee ground (SCG) extract

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1303-1311
Author(s):  
N.A. Shukri ◽  
Z. Mohd Zin ◽  
N. MohdMaidin ◽  
Hasmadi M. ◽  
M.K. Zainol
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Spent Coffee Ground ◽  
Coffee Ground ◽  
Phenolic And Flavonoid

Spent coffee ground (SCG) is the by-product of coffee processing that is produced up to 45% in coffee beverage and instant coffee processing and is believed to contain high amounts of antioxidants. Despite the fact that SCG has exerted many advantages, the information on obtaining the antioxidant values using enzymes are still scarce. The objective of the study was therefore to determine the total phenolic and flavonoid content of antioxidant activity and antimicrobial activity in Arabica SCG extracted using pectinase at different pH values. Arabica SCG was extracted using pectinase at pH 3, pH 4, pH 5, pH 6 and pH 7 and analyzed for its anti-microbial activity and antioxidant properties (DPPH, FRAP, total phenolic and total flavonoid content along with individual flavonoids using HPLC). Arabica SCG was extracted using pectinase at pH 3, pH 4, pH 5, pH 6 and pH 7 and analyzed for its antimicrobial activity and antioxidant properties (DPPH, FRAP, total phenolic and total flavonoid content along with individual flavonoids using HPLC). The result showed that the antioxidant capacity of the SCG extract at pH 4 exhibited higher DPPH and FRAP values. The total phenolic and flavonoid content exhibited in (1.38±4.42, mg GAE/g sample and 22.57±0.27 mg QE/g of dry sample). Flavonoids namely quercetin, kaempferol, rutin, gallic acid, catechin, epigallocatechin gallate, pcoumaric acid and myricetin were present in all samples at various levels. The SCG in pH 7 extract showed the highest concentrations of the individual flavonoid compound in the sample and the highest inhibition zone on Pseudomonas aeruginosa and Staphylococcus aureus. Thus, the optimum pH of the pectinase which is pH 4-5 had provided the highest yield in antioxidant capacity and activity of the Arabica SCG extract with this extraction method that can be used for food preservation as well as in the nutraceutical industry.

scholarly journals In-vitro antioxidant, anti-cancer, and anti-inflammatory activities of selected medicinal plants from western Nepal

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Bishnu Prasad Pandey ◽  
Kapil Adhikari ◽  
Suman Prakash Pradhan ◽  
Hee Jeong Shin ◽  
Eun Kyeong Lee ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antioxidant Properties ◽  
Melia Azedarach ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Flavonoid Content ◽  
Anti Inflammatory

Abstract Background This study was focused on the measurement of anticancer properties of six medicinal plants from western Nepal in three cell lines; HeLa, Hep3B, and HCT116, and anti-inflammatory properties in RAW 264.7 cell line through NO, PGE2, and TNF-α production. In addition, the phytochemical screening, total phenolic, flavonoid content, and antioxidant properties were evaluated. Results The qualitative phytochemical analysis revealed the presence of different secondary metabolite and range of total phenolic and total flavonoid content. The highest antioxidant activities were observed in Bergenia pacumbis against both DPPH (IC50 = 25.97 ± 0.19 μg/mL) and ABTS (IC50 = 14.49 ± 0.40 μg/mL). Furthermore, the highest antiproliferative effect against cervical, liver, and colon cancer cell lines were observed in Melia azedarach as IC50 values of 10.50, 5.30, and 1.57 μg/mL respectively, while the strongest anti-metastatic potential on liver cancer cell line was found in Pleurospermum benthamii. In addition, P. benthamii showed the most potent anti-inflammatory effect in RAW264.7 murine macrophage cells. Conclusion This study provided the evidence for M. azedarach and P. benthamii to have great anticancer potential and finding builds the enough scientific backgrounds in future to isolate and purify the bioactive compounds for further applications.

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