scholarly journals Prospects and methods of polyploid selection of new high-yielding miscanthus clones in the natural climatic conditions of Ukraine

Bioenergy ◽  
2021 ◽  
Author(s):  
M. V. Roik ◽  
N. S. Kovalchuk ◽  
O. A. Zinchenko ◽  
M. Ya. Humentyk ◽  
H. S. Honcharuk
Keyword(s):  
Genetic Basis ◽  
Pollen Grains ◽  
Exposure Period ◽  
Climatic Conditions ◽  
Miscanthus Sinensis ◽  
Nutrient Media ◽  
Miscanthus Sacchariflorus ◽  
Second Year ◽  
Tetraploid Level ◽  
Selection Of

Goal. Expanding the breeding trait collection of breeding genotypes of the genus Miscanthus (Anderson) and the genetic basis for new allotriploid clones by transferring components for hybridization of natural wild species Miscanthus sinensis and Miscanthus sacchariflorus to the tetraploid level. Methods. Cytological, biotechnological, fluorescent cytphotometry, field, laboratory. Results. The efficiency of polyploidisation for induction of new tetraploid forms of miscanthus in liquid nutrient media supplemented with colchicine (0.05% mass) and for stabilization of myxoploid shoots (0.005% mass) for 6 h of cultivation is examined. The period of exposure for Miscanthus sinensis (2x=2x= 38) and Miscanthus sacchariflorus (2x = 2x = 38) varied from 2 hours to 3 days depending on the genetic origin of the material, with transfer to a hormonal environment. The best indicators of tetraploid induction for Miscanthus sinensis were observed for the exposure period of 2 days with polyploidization efficiency (Db%) of 31.25% and 21.42%, and for Miscanthus sacchariflorus 2 and 6 hours with 35.0% and 27.3%, respectively. The technology of transfering rooted shoots into the soil on the Yaltushkiv Eperimental Breeding Farm was improved and the composition of the soil mixture that provided 99% rooting of culture seedlings at a humidity of 60−70% and air temperature of 35−55°С was found. Conclusions. New biotechnological tetraploid lines of Miscanthus sinensis (2xn=4x=76) and Miscanthus sacchariflorus (2xn=4x=76) were created in the conditions of liquid nutrient media supplemented with colchicine (0.05 % mass). It is investigated that flowering of new tetraploid clones in the conditions of Ukraine for the second year of vegetation occurs from late September to early October with formation of fertile pollen grains.

scholarly journals PECULIARITIES OF CREATION OF MISCANTHUS SINENSIS AND MISCANTHUS SACCHARIFLORUS TETRAPLOID LINES

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Maliarenko O.A. ◽  
Maliarenko O.A. ◽  
Maliarenko O.A. ◽  
Maliarenko O.A. ◽  
Maliarenko O.A. ◽  
...  
Keyword(s):  
Computer Software ◽  
Pollen Grains ◽  
Exposure Period ◽  
Miscanthus Sinensis ◽  
Miscanthus Sacchariflorus ◽  
Status Differentiation ◽  
Breeding Schemes ◽  
Second Year ◽  
Tetraploid Level ◽  
Genome Ploidy

The work aimed at creating tetraploid lines of Miscanthus sinensis and Miscanthus sacchariflorus species. To achieve this goal, we used methods of microclonal propagation, fluorescence cytophotometry, and genomic status differentiation using computer software of AP ‘Partec’ (Germany). It was found that the percentage of cultivated shoots of Miscanthus sacchariflorus for an exposure period of 1 day was 18.86 ± 5.37%. To compare, it was51.78 ± 6.51% in Miscanthus sinensis. The best indicators of tetraploid induction in Miscanthus sinensis were observed for the exposure to colchicine for 2 days with polyploidization efficiency of 31.25% and 21.42%, and in Miscanthus sacchariflorusf or 2 hours and 6 hours with rates of 35.0% and 27.3%, respectively. To stabilize the tetraploid level of genome ploidy, we used Murashige and Skoog liquid media (1962) supplemented with 0.005% colchicine and an exposure period of myxoploids for 6 hours. The flowering of new tetraploid clones in the conditions of Ukraine was observed on the second year of vegetation in late September and the beginning of October with the formation of fertile pollen grains. However, development of a microgametophyte depends on temperature conditions, both for Miscanthus sinensis (4x) and Miscanthus sacchariflorus (4x). Breeding schemes for the formation of anisoploid populations have also been developed: M sinensis (4x) x M sinensis (2x); M. sa?chariflorus (4x) x M sinensis (2x); M sinensis (4x) x M. sachariflorus (2x)

Some influence of the hormonal components in nutrient medium onthe induction of novel structures when obtaining rapeseed haploids through in vitro culture

2018 ◽  
pp. 30-37
Keyword(s):  
Anther Culture ◽  
Hormonal Regulation ◽  
Control Plant ◽  
Pollen Grains ◽  
Control Treatment ◽  
Basic Medium ◽  
Nutrient Media ◽  
Oilseed Crops ◽  
Wide Range

Growth regulators, phytohormones, both natural and artificial, are the main means to control plant ontogenesis. They are involved in regulating the processes of cell differentiation and cell divisions, the formation of tissues and organs, the changes in the rate of growth and development, the duration of the certain stages of ontogenesis. The main classes of phytohormones used in plant biotechnology, in particular, in the induction of haploid structures, are auxins and cytokinins. The mechanism of action of phytohormones on a cell is rather complicated and may have a different character. Understanding the characteristics of the action of phytohormones is complicated by the fact that the system of hormonal regulation of plant life is multicomponent. This is manifested in the fact that the same physiological process is most often influenced not by one, but by several phytohormones, covering a wide range of aspects of cell metabolism. In connection with the foregoing, the purpose of our work was to test a set of nutrient media with different basic composition and different proportions of phytohormones to determine the patterns of their influence on the processes of haploid structure induction in rape anther culture using accessions, developed at the Institute of Oilseed Crops NAAS. The material used was two accessions of winter rapeseed (No. 1 and No. 2) and one sample of spring rapeseed, provided by the Rapeseed Breeding laboratory of the Institute of Oilseed Crops. Incised inflorescences were kept against the background of low temperature of 6–8 ° C for several days, and then, under aseptic conditions, anthers with unripe pollen grains were isolated and planted on nutrient media differing in both basic mineral composition and content of phytohormones. MS (Murashige & Skoog 1962) and B5 (Gamborg et al 1968) media were used as basic media. Phytohormones were added to the basic media in various combinations – BA, 2,4-D, NAA at the concentrations of 0.1-0.6 mg/l. In each treatment up to 300 anthers were cultivated. Differences between treatments were evaluated using standard t-test. Studies have shown that in the anther culture of rapeseed on the tested nutrient media, morphogenic structures of different types (embryoids and callus) were originated. Synthetic auxin 2,4-D, regardless of the composition of the basic medium, caused the formation of structures of both types, though with a low frequency. Phytohormone BA of the cytokinin type had a similar effect. In this case, the frequency of structures was slightly higher, and the developed structures were represented mainly by embryoids. The joint action of cytokinin and auxin was the most favorable for the initiation of morphogenic structures. Such combination of phytohormones caused the formation of these structures with a frequency of 24.5-14.7% in the studied genotypes of winter rape. A similar effect of phytohormones on the induction and development of morphogenic structures was also observed in spring rape. In this case, a single basic MS medium was used. The experiment included treatments where phytohormones were absent (control), as well as various combinations of auxin and cytokinin. In the control treatment, the formation of new structures was not noted. In treatments with phytohormones, in addition to the medium with the combination of auxin and cytokinin, the medium in which only cytokinin was present was also rather effective. The treatment in which the action of auxin 2,4-D was combined with the action of another auxin, NAA, turned out to be practically ineffective. Thus, it was found that for the induction of morphogenic structures from microspores in rape anther culture of the tested genotypes, the combination of cytokinin with auxin, or the use of only single cytokinin BA without other phytohormones, had the most positive effect.

scholarly journals Potential for Aquifer Storage and Recovery (ASR) in South Bihar, India

2021 ◽  
Vol 13 (6) ◽  
pp. 3502
Author(s):  
Somnath Bandyopadhyay ◽  
Aviram Sharma ◽  
Satiprasad Sahoo ◽  
Kishore Dhavala ◽  
Prabhakar Sharma
Keyword(s):  
Pilot Study ◽  
Environmental Sustainability ◽  
Hard Rock ◽  
Climatic Conditions ◽  
Aquifer Storage And Recovery ◽  
Aquifer Recharge ◽  
Aquifer Storage ◽  
Cropping Season ◽  
Selection Of ◽  
Surface Technique

Among the several options of managed aquifer recharge (MAR) techniques, the aquifer storage and recovery (ASR) is a well-known sub-surface technique to replenish depleted aquifers, which is contingent upon the selection of appropriate sites. This paper explores the potential of ASR for groundwater recharge in the hydrological, hydrogeological, social, and economic context of South Bihar in India. Based on the water samples from more than 137 wells and socio-economic surveys, ASR installations were piloted through seven selected entrepreneurial farmers in two villages of South Bihar. The feasibility of ASR in both hard rock and deep alluvial aquifers was demonstrated for the prominent aquifer types in the marginal alluvial plains of South Bihar and elsewhere. It was postulated through this pilot study that a successful spread of ASR in South Bihar can augment usable water resources for agriculture during the winter cropping season. More importantly, ASR can adapt to local circumstances and challenges under changing climatic conditions. The flexible and participatory approach in this pilot study also allowed the farmers to creatively engage with the design and governance aspects of the recharge pit. The entrepreneurial farmers-led model builds local accountability, creates avenues for private investments, and opens up the space for continued innovation in technology and management, while also committing to resource distributive justice and environmental sustainability.

Research changes in the properties of butadiene-nitrile rubber under various aging conditions

2021 ◽  
pp. 009524432110290
Author(s):  
Mariya L Davydova ◽  
Aytalina F Fedorova
Keyword(s):  
Accelerated Aging ◽  
Physical And Mechanical Properties ◽  
Exposure Period ◽  
Climatic Conditions ◽  
Nitrile Rubber ◽  
Sterically Hindered Phenols ◽  
Loading Mode ◽  
Hindered Phenols ◽  
The Republic ◽  
Butadiene Nitrile Rubber

This article represents the results of a study of changes in the properties of vulcanizates based on BNR-18 butadiene-nitrile rubber containing as stabilizers the experimental spatially hindered phenols Stafen, CO3, CO4, and industrial antioxidant 6PPD, after accelerated aging (100°C 96 h) and aging under full-scale exposure in extreme climatic conditions of the Republic of Sakha (Yakutia) during 2 years. In winter, the air temperature reached—48°C, in summer—+36.1°C. It is shown that the experimental sterically hindered phenols more effectively under natural exposure conditions. They are characterized by the most stability in terms of strength throughout the entire exposure period. Under conditions of accelerated aging, the vulcanizate containing the industrial antioxidant 6PPD is characterized by the greatest stability of physical and mechanical properties. According to the viscoelastic characteristics obtained in the dynamic loading mode, the contribution of the presented stabilizers in maintaining resistance to temperature and deformation effects compared with unstabilized rubber is confirmed.

PSXII-2 Identification of candidate SNPs associated with high prolificacy in Romanov sheep

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 254-255
Author(s):  
Alexey V Shakhin ◽  
Arsen V Dotsev ◽  
Tatiana E Deniskova ◽  
Gottfried Brem ◽  
Natalia A Zinovieva
Keyword(s):  
Higher Education ◽  
Genetic Basis ◽  
Reference Genome ◽  
Unique Property ◽  
Candidate Snps ◽  
Merino Sheep ◽  
Sheep Breeds ◽  
Important Trait ◽  
Very High ◽  
Selection Of

Abstract Prolificacy is a very important trait in sheep. Romanov sheep, the well-known Russian sheep breed, are characterized by very high prolificacy; however, the genetic basis of this unique property of Romanov sheep is still unknown. It was reported that Ovine BMPR1B gene, located on the OAR6, is associated with prolificacy in several sheep breeds. The aim of our study was to identify candidate SNPs within BMPR1B gene, related to prolificacy. To achieve this goal, using NGS technology, we sequenced ovine BMPR1B gene in Romanov sheep (n = 6), which are characterized by high prolificacy (about 270 lambs per 100 ewes). The sequences of BMPR1B gene of Noire du Velay, Tan, Southdown and Australian Horned Merino sheep breeds as well as Asiatic mouflon (n = 1), which are characterized by significantly lower prolificacy (from 110 to 180 lambs per 100 ewes) were derived from publicly available sources and used for comparison. FST analysis performed in PLINK 1.9 program revealed 10 SNPs with values higher than 0.8. The majority of candidate SNPs under putative selection were localized in the region from 29,382,098 to 29,430,387 on OAR6 of Ovine reference genome (Oar_v3.1 (Ensembl release 98). Thus, we can suggest, that this region of the BMPR1B gene can be considered as the putative region, associated with high prolificacy of Romanov sheep. Additional studies will be needed to confirm the effect of identified candidate SNPs on prolificacy traits. The research results will be useful for artificial selection of sheep with higher prolific capacity, including the introduction of desired alleles in sheep populations using genome editing technologies. This work was supported by the Russian Ministry of Science and Higher Education No. 0445-2019-0024 and RFBR No. 20-516-56002.

scholarly journals Elk (Cervus elaphus) Seasonal Habitat Selection in a Heterogeneous Forest Structure

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jesse N. Popp ◽  
David N. C. McGeachy ◽  
Josef Hamr
Keyword(s):  
Habitat Selection ◽  
Cervus Elaphus ◽  
Forest Structure ◽  
Hardwood Forests ◽  
Climatic Conditions ◽  
White Birch ◽  
Balsam Poplar ◽  
Adult Females ◽  
Selection Of ◽  
Future Management

Seasonal habitat selection by the reintroduced Burwash elk population, approximately 30 km south of Sudbury, Ontario, has been analysed in order to assist in the development of future management. Twenty-five adult females were radio-collared and tracked 1–3 times a week for 3 years. The most prominent patterns included selection of intolerant hardwood forests (trembling aspen, white birch, and balsam poplar) during all seasons, while Great Lakes-St. Lawrence pines (white and red pine dominated stands) were used less than expected based on availability for all seasons. The selection patterns are likely associated with seasonal climatic conditions and forage preferences. Because the selection behaviours displayed here varied greatly from other elk habitat studies, it is suggested that managers consider the importance of population-specific habitat studies before developing related strategies.

scholarly journals Prunus necrotic ringspot virus Early Invasion and Its Effects on Apricot Pollen Grain Performance

2007 ◽  
Vol 97 (8) ◽  
pp. 892-899 ◽  
Author(s):  
Khalid Amari ◽  
Lorenzo Burgos ◽  
Vicente Pallas ◽  
María Amelia Sanchez-Pina
Keyword(s):  
Developmental Stages ◽  
Generative Cell ◽  
Pollen Grains ◽  
Growth Zone ◽  
Pollen Tubes ◽  
Climatic Conditions ◽  
Pollen Grain ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus

The route of infection and the pattern of distribution of Prunus necrotic ringspot virus (PNRSV) in apricot pollen were studied. PNRSV was detected both within and on the surface of infected pollen grains. The virus invaded pollen during its early developmental stages, being detected in pollen mother cells. It was distributed uniformly within the cytoplasm of uni- and bicellular pollen grains and infected the generative cell. In mature pollen grains, characterized by their triangular shape, the virus was located mainly at the apertures, suggesting that PNRSV distribution follows the same pattern as the cellular components required for pollen tube germination and cell wall tube synthesis. PNRSV also was localized inside pollen tubes, especially in the growth zone. In vitro experiments demonstrated that infection with PNRSV decreases the germination percentage of pollen grains by more than half and delays the growth of pollen tubes by ≈24 h. However, although PNRSV infection affected apricot pollen grain performance during germination, the presence of the virus did not completely prevent fertilization, because the infected apricot pollen tubes, once germinated, were able to reach the apricot embryo sacs, which, in the climatic conditions of southeastern Spain, mature later than in other climates. Thus, infected pollen still could play an important role in the vertical transmission of PNRSV in apricot.

scholarly journals THE ESTIMATION OF PEAS COLLECTION IN THE ALTAI

2019 ◽  
pp. 48-52
Author(s):  
S. K. Shchukis ◽  
E. R. Shchukis
Keyword(s):  
Plant Height ◽  
Rapid Development ◽  
Climatic Conditions ◽  
Source Material ◽  
Pea Aphid ◽  
Diverse Source ◽  
Selection Of ◽  
Vast Territory

The article emphasizes the importance of peas as a source of vegetative protein, and indicates on insufficient areas under peas both in Russia and in the Altai. One of the reasons of reducing its acreage is the high costs on peas planting and protecting from pests, diseases and weeds. Climate changing contributes to rapid development of such harmful diseases as ascohitoz, bacteriosis, fusarium, rust, significant number of weevil, pea aphid and moth mullet. Considering the main directions in pea breeding, it should be emphasized that the Altai Area, with its vast territory and diverse natural and climatic conditions, requires highly productive, well-adapted varieties of different use which are capable to effectively use agro-climatic resources of different zones. All this requires the active study of the source material, the selection of new, economically valuable forms and their introduction into the breeding process. It has been noted that the production requires more advanced varieties, the development of which is impossible without the presence of diverse source material. The purpose of the study was to identify the best productive plants in the pea collection of 2012-2017. The material has been represented by 113 variety samples of domestic and foreign selection. The varieties with best productivity were ‘Mnogoplodny 163’, ‘Rheinperle’, ‘Flagman 12’, ‘806’9, ‘Varyag’, ‘Altaisky Usaty’, ‘Pioner’, ‘LittleMarwel’, ‘Mnogoplodny 27’, ‘Zeleny Tsukat’, ‘Ryadovoy’, ‘576/80’. The best forage productivity was produced by ‘Novosibirets’, ‘Varyag’, ‘Pino’, ‘Zeleny Tsukat’, ‘Mnogoplodny 105’. The most fast-ripening varieties were ‘Rheinperle’ and ‘Pyrama’. The samples ‘Novosibirets’, ‘Varyag’, ‘Pino’ and ‘8067’ were characterized with high plant height. The samples ‘Pyrama’, ‘Flagman 12’, ‘Novosibirets’, ‘Sihirobana-Kinusaja’, the line ‘312’ produced large-sized kernels. The great lodge resistance has been shown by ‘Altaisky Usaty’, ‘Svetozar’, ‘Kamerton’, ‘Flagman 12’.

scholarly journals The First Korean Garden in Russia

2021 ◽  
pp. 102-112
Author(s):  
Victor Kuzevanov ◽  
Alexey Ponomarev ◽  
Sergey Kalyuzhny ◽  
Yong-Shik Kim
Keyword(s):  
Science Education ◽  
International Cooperation ◽  
Climatic Conditions ◽  
State University ◽  
Design Development ◽  
Botanic Garden ◽  
Garden Design ◽  
Natural Heritage ◽  
History Of ◽  
Selection Of

The history of the first «Korean Garden» design, development and establishment within the Irkutsk State University Botanic Garden in the harsh climatic conditions of Baikalian Siberia is described. The peculiarities of the selection of plants and landscape arrangements represent this garden as a unique ethnobotanical object – the cultural and natural heritage of Korea, an ecological and humanitarian resource for science, education and international cooperation.

Sign in / Sign up

Export Citation Format

Share Document