scholarly journals Pharmaceutical Analysis of An Anti-Diabetic Formulation: Kimshukatvagadi Ghana Capsule

2021 ◽  
Vol 12 (2) ◽  
pp. 247-250
Author(s):  
Garima Singh ◽  
Shailesh V Deshpande ◽  
Rinjin G Krishna
Keyword(s):  
High Performance ◽  
Curcuma Longa ◽  
Large Population ◽  
Herbal Medicines ◽  
Chemical Components ◽  
Butea Monosperma ◽  
Solvent Systems ◽  
Strychnos Potatorum ◽  
Layer Chromatography ◽  
Salacia Reticulata

Ayurveda is one of the oldest and holistic science. Herbal medicines have a long therapeutic history; serving many of the health needs of large population of the world. However, the quality control and assurance remains as a challenge due to the high discrepancy of chemical components involved. In Ayurvedic texts, several formulations have been mentioned in Prameha (Diabetes Mellitus). Kimshukatvagadi is one such formulation mentioned in Sahasrayoga Vati Prakarana adhyaya. It contains Palash (Butea monosperma Lam.), Haridra (Curcuma longa L.), Amalaki (Emblica officinalis L.), Kataka (Strychnos potatorum L.f.), Vairi (Salacia reticulata Wight). Kimshukatvagadi Vati was converted into Ghana to increase its potency and then it was sealed into Capsule for increasing the shelf life, making it easy to dispense, dose fixation etc. Kimshukatvagadi Ghana Capsule was subjected to organoleptic analysis, phytochemical and qualitative analysis to detect the presence of various functional groups, and to high performance thin layer chromatography (HPTLC) examination by optimizing the solvent systems.

Squalene metabolism in two species of Lagenidium

1994 ◽  
Vol 40 (7) ◽  
pp. 523-531 ◽  
Author(s):  
Aristotle Domnas ◽  
Shankha S. Biswas ◽  
Patricia A. Gallagher
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Positive Control ◽  
Gas Liquid Chromatography ◽  
Supporting Evidence ◽  
Lagenidium Giganteum ◽  
Solvent Systems ◽  
The Stability ◽  
Layer Chromatography

Squalene metabolism of the sterol auxotroph Lagenidium giganteum was studied and compared with that of the positive control Lagenidium callinectes. Application of experimentally derived precautions ensured both the stability and the purity of squalene during incubations. Under these conditions mycelia of L. giganteum converted squalene to squalene oxide and to a sterol-like compound. Cell-free and microsomal preparations also converted squalene to the oxide, which was identified by thin layer chromatography with five different solvent systems, by co-chromatography with authentic oxide, and by conversion to the glycol. Supporting evidence for the production of squalene oxide was obtained by gas–liquid chromatography, high performance liquid chromatography, and autoradiography. The squalene oxide produced was identified by mass spectrometry.Key words: Lagenidium giganteum, Lagenidium callinectes, squalene, 2,3-squalene epoxide.

scholarly journals Variation of Ginsenosides in Ginseng of Different Ages

2016 ◽  
Vol 11 (6) ◽  
pp. 1934578X1601100
Author(s):  
Jian-Ming He ◽  
Yi-Zhen Zhang ◽  
Jian-Ping Luo ◽  
Wen-Ju Zhang ◽  
Qing Mu
Keyword(s):  
High Performance ◽  
Herbal Medicines ◽  
Ginseng Root ◽  
Chemical Components ◽  
General Knowledge ◽  
Different Ages ◽  
Medicinal Properties ◽  
Major Chemical ◽  
Wild Ginseng

Panax ginseng has been used in traditional oriental medicine for thousands of years. Ginsenosides, the major chemical components of the roots, are considered to be responsible for the medicinal properties of ginseng. Ginsenosides increase with the age of ginseng root in general knowledge, and in this study the content of ginsenosides in ginseng of different ages was quantified. Separation and determination of eight main ginsenosides, Rgl, Re, Rbl, Re, Rg2, Rb2, Rb3 and Rd, was performed by high performance liquid chromatography with UV detection at 203 nm. The content of Rgl, Re, Rbl, Re, Rg2 and Rd increased from 5 to 16-year-old ginseng and then decreased, while Rb2 and Rb3 increased in the range of 5-12 years, but then slowly decreased. However, the total eight ginsenosides in 16 year old ginseng had a higher content than that in any other from 5-18 years old. As a result, the content of ginsenosides and total ginsenosides was not positively related to age from 5-18 years, which is not in full agreement with the general knowledge of ginseng. Thus, this study suggests that the older wild ginseng may not result in better medicinal ginseng for herbal medicines.

Simultaneous Quantification of Pharmacological Markers Quercetin and Berberine Using High-Performance Thin-Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC) from a Polyherbal Formulation Pushyanuga Churna

2019 ◽  
Vol 102 (4) ◽  
pp. 1003-1013 ◽  
Author(s):  
Sunita Shailajan ◽  
Yugandhara Patil ◽  
Mayuresh Joshi ◽  
Sasikumar Menon ◽  
Mandar Mhatre
Keyword(s):  
High Performance ◽  
Herbal Medicines ◽  
Holistic Approach ◽  
Great Promise ◽  
Chromatographic Techniques ◽  
Chromatographic Fingerprints ◽  
Mainstream Medicine ◽  
Pharmacologically Active ◽  
Layer Chromatography

Abstract Background: Ayurvedic medicines show great promise due to their holistic approach in the treatment of diseases. But proper standardization is necessary for their integration into mainstream medicine. One such well-known formulation is Pushyanuga Churna. As safety and efficacy of a multiherbal formulation is dependent on the authenticity of the ingredients used, chromatographic techniques play a significant role in the quality control of complex herbal medicines. Objective: In the current research, marker-based standardization of Pushyanuga Churna using validated HPTLC and HPLC methods have been established. Methods: Pushyanuga Churna was prepared in-house using authentic ingredients. Pharmacologically active biomarkers quercetin and berberine were simultaneously estimated using validated HPTLC and HPLC methods. Chromatographic fingerprints were developed for the formulation along with all the ingredients that can be used as tools to identify Pushyanuga Churna. Results: In-house formulation and Patanjali (marketed formulation) were observed to be rich in both markers. Quercetin was found to be more abundant than berberine in the ingredients. The deviation of marker content in marketed formulations can be attributed to variation in the ingredients used in the preparation. Conclusions: The routine use of such scientifically accepted methods will help in establishing the quality of the formulation and help in building confidence in traditional medicine. Highlights: The study highlights evaluation of biomarkers from the formulation and its ingredients as an important method to ensure the batch to batch consistency in quality of the formulation.

scholarly journals Review on Plants Fingerprinting and Tlc Fingerprinting of Two Aqueous Herbal Products

2018 ◽  
Vol 3 (3) ◽  
Keyword(s):  
Systematic Review ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chemical Method ◽  
Herbal Medicines ◽  
Common Type ◽  
Herbal Products ◽  
Herbal Preparations ◽  
Layer Chromatography

As herbal medicines have become commercialized, the safety, quality and efficacy of medicinal plants and herbal products have become of great concern. In view of this a review on Plant fingerprinting was carried out using systematic review and TLC fingerprinting was also carried out on two herbal products using simple Thin Layer Chromatography. The results revealed Morphological, Chemical and genetic methods are the major types studied. The High Performance Thin Layer Chromatography (HPTLC) is the most common type to chemical method utilized and most of the studies on Plants fingerprinting were carried out in Asia. Comparing the TLC profile of the two herbal preparations reveals that the two products are basically the same

scholarly journals Pharmacognostical and Pharmacochemical Parameters of Tablet Sutashekhara Rasa – Without Gold

2015 ◽  
Vol 3 (01) ◽  
pp. 64-70
Author(s):  
Shweta P. Mata ◽  
D. B. Vaghela ◽  
K. S. Dhiman ◽  
C. R. Harisha ◽  
V. J. Shukla
Keyword(s):  
High Performance ◽  
Ph Value ◽  
Beta Blockers ◽  
Modern Medicine ◽  
Physicochemical Analysis ◽  
Average Weight ◽  
Average Hardness ◽  
Temporary Relief ◽  
Solvent Systems ◽  
Layer Chromatography

Tablet Sutashekhara Rasa (TSR) is an Ayurvedic, herbo-metal formulation prescribed widely for several conditions such as Acid peptic disorders, Pain in abdomen, Haemorrhage, Mental disorders etc. On analysis of pharmcodynamics of this compound it is basically Pitta corrective drug. Ardhavabhedaka (Migraine) is also one of the clinical morbidity which is manifested by vitiated Pitta/Rakta along with Vata. The available treatment in modern medicine is use of NSAIDs, Beta-blockers etc. with only temporary relief. TSR being a Pitta corrective is used in a clinical study with new indication in Ardhavabhedaka (Migraine). Till date there is no data available regarding evaluation ofTSR. Present study an attempt to develop newer approaches for the quality control and standardization of TSR. The samples were subjected to organoleptic, physicochemical analysis and Chromatographic (HPTLC) examination by optimizing the solvent systems. The phrmacognostical study of ingredients of TSR shows the presence of Sceleriform vessel, Lignified stone cells, Bottle necked shapedstone cells etc. Pharmaceutical analysis showed that the Average weight of tablet 276mg, Average hardness of tablet 2.05 Kg/cm2 , Loss on drying 4.7904% w/w, pH value 7 and High Performance Thin Layer Chromatography at 254nm and 366nm resulted into 6 spots.

scholarly journals Validated high-performance thin-layer chromatographic analysis of curcumin in the methanolic fraction of Curcuma longa L. rhizomes

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Poonam Kushwaha ◽  
Babita Shukla ◽  
Jyotsana Dwivedi ◽  
Sumedha Saxena
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Curcuma Longa ◽  
Dry Weight ◽  
Good Separation ◽  
Chromatography Method ◽  
Crude Drugs ◽  
Thin Layer Chromatographic Analysis ◽  
Layer Chromatography ◽  
Absorption Mode

Abstract Background In the present study, an HPTLC (high-performance thin-layer chromatography) method was developed for the quantitative determination and validation of the curcumin in the methanolic fraction of Curcuma longa L. For achieving good separation of curcumin, the mobile phase of chloroform:methanol (97:3) was used. The densitometric analysis of curcumin was performed at 420 nm in reflection/absorption mode. Results Linearity of the method was obtained in the range of 100‒600 ng per spot. During analysis, the methanolic fraction of the C. longa showed the presence of a quantifiable amount of curcumin. The content of curcumin was found to be 1.5% (per dry weight). Conclusions The method is specific, simple, precise, and accurate. The obtained data can have used for the routine analysis of the reported biomarkers in crude drugs and extracts. The quantification and the method validation of curcumin have not yet been reported in C. longa which can be utilized for the proper standardization of the plant.

scholarly journals Standardization of the Ayurvedic Formulation Haridra Khanda Using High-Performance Thin-Layer Chromatography-Densitometry

2008 ◽  
Vol 91 (5) ◽  
pp. 1162-1168 ◽  
Author(s):  
Kedar Kumar Rout ◽  
Sagarika Parida ◽  
Sagar Kumar Mishra
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Curcuma Longa ◽  
Water Soluble ◽  
Trace Metal Analysis ◽  
Cell Structures ◽  
Microscopic Evaluation ◽  
Multiple Wavelengths ◽  
Layer Chromatography ◽  
Instrumental Precision

Abstract The present study aimed to standardize the Ayurvedic preparation Haridra Khanda containing Curcuma longa as a major ingredient. Various physicochemical parameters such as alcohol-soluble extractive, water-soluble extractive, total ash, and acid-insoluble ash were determined according to the Ayurvedic Pharmacopoeia of India. Microscopic evaluation of the formulation revealed the presence of various diagnostic cell structures of C. longa. Trace metal analysis indicated the absence of toxic metals such as As, Cd, Hg, and Pb. High-performance thin-layer chromatographic (HPTLC) fingerprint patterns at multiple wavelengths (254, 366, and 430 nm) identified the number of components present at each wavelength. The bioactive markers curcumin (C1), demethoxycurcumin (C2), and bisdemethoxycurcumin (C3) were quantified by using a simple, rapid, and efficient HPTLC method using plates precoated with silica gel 60F254 stationary phase. The instrumental precision [coefficient of variation (CV)] was 0.51, 0.64, and 0.79% and the repeatability of the method (CV) was 0.89, 1.11, and 0.95%, respectively, for C1 to C3. Limits of detection and quantitation for compounds C1 to C3 were 20, 20, and 15 ng and 50, 40, and 50 ng, respectively. Response was a linear function in the ranges of 50350, 40240, and 50300 ng with correlation coefficient (r) 0.9998, 0.9995, and 0.9992, respectively, for C1 to C3. The mean recovery values of 99.63 (C1), 98.65 (C2), and 98.97% (C3) indicated the excellent accuracy of the method. It is shown that HPTLC can be applied successfully for the marker evaluation of the formulation containing C. longa.

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