scholarly journals Microbiological and Immunological Studies on Brucellosis in a Hospital in Al-Madinah Al-Munawarah

2020 ◽  
Vol 1 (2) ◽  
pp. 24-44
Author(s):  
Manal Mohamed Elsayed Ahmed ◽  
Ibrahim A. ◽  
Abd El-Rahman M.
Keyword(s):  
Saudi Arabia ◽  
Antibody Titer ◽  
Armed Forces ◽  
Raw Milk ◽  
Agglutination Test ◽  
Diagnostic Methods ◽  
Human Brucellosis ◽  
Female Ratio ◽  
Titer Group ◽  
Prevalent Species

Purpose: The study was conducted to estimate the prevalence of brucellosis in Prince Sultan Armed Forces Hospital at Al-Madinah Al-Munawarah, Saudi Arabia. The aim was also to determine the most prevalent species of Brucella and to make a comparison between culture and serological methods in diagnosis and to evaluate the levels of sIL-2R and/or IFN-γ production to be used as markers of treatment efficacy. Study Design: Cross-sectional Study Subjects and Methods: This study was conducted on 65 patients with male: female ratio (2:1) suspected of having brucellosis. It was carried out using slide agglutination test for detection of anti-Brucella antibodies.  Also, we estimated anti-Brucella IgG and IgM antibody levels in the sera of examined patients using ELISA. Quantization of human IFN-ɣ was performed. Results: The total incidence of brucellosis was 92.3%. The incidence among males (95.2%) was higher than that of female (87%). Brucellosis was detected in all age groups. Most of brucellosis patients were recovered during the period from January to June. Consumption of milk products, heating raw milk and milking animals were the highest risks with an incidence of 100% followed by drinking raw milk with an incidence of 95% while cutting raw meat and animal contact were the less risk with an incidence of 80% and 67%, respectively. The most prevalent species among examined patients was B. melitensis (86%) and B. abortus (6%). Brucellosis patients had 63% and 83%of anti BrucellaIg G and IgM, respectively. The highest (%) of patients having positive IgG and IgM levels in their sera were among 1/160 standard tube agglutination test (SAT) antibody titer group brucellosis patients were having positive levels of IFN-ɣ. All of them belonged to 1/80 antibody titer group. The mean IFN-ɣ levels according to SAT antibody titers were 224.25, 102 and 69.3 pg/ml, respectively. Conclusion: Eradication of human brucellosis depends on the eradication of animal brucellosis. In countries like the Kingdom of Saudi Arabia, where brucellosis is endemic; rapid, sensitive and highly specific diagnostic methods are required to make early diagnosis and prevent resistance as there is an overlap in therapy.

scholarly journals Seroprevalence of Human Brucellosis in Wadi Al Dawaser region of Saudi Arabia

2018 ◽  
Vol 35 (1) ◽  
Author(s):  
Mohamudha Parveen Rahamathulla
Keyword(s):  
Risk Factors ◽  
Saudi Arabia ◽  
Predictive Value ◽  
Diagnostic Yield ◽  
Public Health Problem ◽  
Human Brucellosis ◽  
Major Public Health Problem ◽  
Female Ratio ◽  
Middle East Countries ◽  
Igm Elisa

Background and Objective: Brucellosis is an important zoonotic disease and a major public health problem in the Middle East countries including Saudi Arabia. This study has evaluated the seroprevalence of human brucellosis in Wadi Al Dawaser region of Central Saudi Arabia. Methods: The study was conducted for three years (2015-2018) at Wadi Al Dawaser general hospital. A total of 6721 clinically suspected serum samples were collected over three years and tested by Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT), IgM and IgG ELISA. A standard questionnaire to determine the risk factors were used among patients. Results: Of the total 6721 samples tested, 576 (8.6%) were seropositive for brucellosis. RBPT identified 427 (74.1%), SAT titers 493 (85.6%) cases. IgM ELISA and IgG ELISA were positive for 501 (86.9%) and 558 (96.8%) cases respectively. Among the positive cases, 77.3% were male with the male to female ratio of 3.3:1. Nearly, 82% were Saudi Nationals. Direct contact with domestic animals and consumption of raw milk were the risk factors identified. No seasonal variation was seen. Diagnostic yield of IgM ELISA showed 86.9% sensitivity and 100% specificity; IgG ELISA showed 96.8% sensitivity and 100% specificity. Both IgG and IgM ELISAs showed 100% Positive predictive value, 98.9% and 95.6% Negative predictive value respectively. Conclusion: A seroprevalence of 8.6% of human brucellosis was documented from this rural region. This is the first report from Wadi Al Dawaser. Frequent surveillance among risk group, vaccination of livestock, creating awareness and health education among the public and school children are recommended. How to cite this:Rahamathulla MP. Seroprevalence of Human Brucellosis in Wadi Al Dawaser region of Saudi Arabia. Pak J Med Sci. 2019;35(1):---------. doi: https://doi.org/10.12669/pjms.35.1.55 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals BRUCELLOSIS

2018 ◽  
Vol 25 (12) ◽  
pp. 1828-1832
Author(s):  
Ali Faraz ◽  
Syed Yousaf Kazmi ◽  
Muhammad Asad Farhan ◽  
Usama Bin Ghaffar ◽  
Sajid Hussain ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Public Health Problem ◽  
Risk Groups ◽  
Agglutination Test ◽  
Human Brucellosis ◽  
Major Public Health Problem ◽  
Seroprevalence Rate ◽  
Serum Samples ◽  
Cross Sectional ◽  
Nomadic Life

Background: Human brucellosis is a zoonotic infectious disease transmitted from domesticated animals to humans. It remains a major public health problem in Saudi Arabia where 7% of the population still maintains a nomadic life style with domestication of animals. Objectives: This study aims to discover the prevalence of brucellosis and Brucella among patients attending a district government hospital in Majmaah, kingdom of Saudi Arabia. Period: 2 years (from 1st February 2016 to 31st January 2018). Design: Cross sectional descriptive. Settings: King Khalid Hospital, Al Majmaah, Saudi Arabia. Materials (Patients) & Methods: Total 1098 serum samples from the patients with clinical suspicion of brucellosis were screened with rapid slide agglutination test (crescent diagnostics Jeddah) for Brucella antibodies and later confirmed by Serum Tube Agglutination Test. Main Outcome Measures: The result of our study showed that the prevalence of brucellosis among patients attending our study is 9.1%. Results: 100 individuals were found to be seropositive with titers ≥1:160. The result of our study showed that the prevalence of brucellosis among patients attending our study is 9.1%The Majority of the patients presented to the medical OPD and orthopedic unit of the hospital. The prevalence of disease in males was found to be higher as compared to females in the current studied group. The age group 21- 40 years, was found to be far more susceptible to this infection. Majority presented with fever and musculoskeletal complaints. The total seroprevalence rate calculated for the patients attending our hospital is 26.50%. Conclusion: Frequent serological surveillance should be carried out in areas that are endemic. Screening of risk groups, imported animals and household members of active brucellosis must be undertaken. This is a crucial epidemiological move allowing for timely diagnosis and control of disease. Limitations: Our prevalence rate represents a specific segment of the population (i.e., those attending the hospital) and not thegeneral population. 

scholarly journals An epidemiological profile of brucellosis in Tabuk Province, Saudi Arabia

2001 ◽  
Vol 7 (4-5) ◽  
pp. 791-798 ◽  
Author(s):  
K. E. Elbeltagy
Keyword(s):  
Saudi Arabia ◽  
Brucella Melitensis ◽  
Signs And Symptoms ◽  
Raw Milk ◽  
Female Ratio ◽  
Agglutination Titre ◽  
Male Female Ratio ◽  
History Of ◽  
Epidemiological Profile ◽  
Milk Ingestion

All 137 brucellosis cases occurring in Tabuk Province, Saudi Arabia in 1997 were studied retrospectively. Brucella agglutination titre of > or = 1/80, or rising titre plus history of typical signs and symptoms were considered evidence of infection. The incidence rate was 34/100, 000, mean age 33.8 +/- 13.9 years [range: 3-72 years] and male: female ratio 1.8: 1. There were 63.5% of cases rurally resident, 58.4% kept animals at home or elsewhere, 27.0% worked with animals and/or on farms, and 88.3% reported a history of raw milk ingestion. The most common infecting agents were Brucella melitensis, B. abortus and B. suis. Splenomegaly and hepatomegaly were detected in 25.5% and 22.6% of cases respectively.

scholarly journals The Diagnosis and Treatment of Impalpable Testes at King Salman Armed Forces Hospital, Tabuk, Saudi Arabia

Cureus ◽  
2020 ◽  
Author(s):  
Mohammad S Mohammad Alnoaiji ◽  
Asmaa Ghmaird ◽  
Eid H Alshahrani ◽  
Fatima A Qaisy ◽  
Rana S Alotaibi ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Armed Forces ◽  
Diagnosis And Treatment

scholarly journals Recent trends of glucose-6-phosphate dehydrogenase deficiency among saudi population in Riyadh city

2019 ◽  
Vol 35 (6) ◽  
Author(s):  
Fatmah Said Abdullah Al Qahtany
Keyword(s):  
Saudi Arabia ◽  
G6pd Deficiency ◽  
Descriptive Analysis ◽  
Saudi Population ◽  
Cross Sectional ◽  
Female Ratio ◽  
Mortality And Morbidity ◽  
King Saud University ◽  
Riyadh City ◽  
Medical City

G6PD deficiency is associated with erythrocyte deficiency in the X-chromosome enzyme. It causes a hematologic syndrome called hemolytic anemia that connects G6PD deficiency with X-linked condition. In the Middle East, including Saudi Arabia, G6PD deficiency is the most dominant genetic blood disorders. It results in higher rates of mortality and morbidity due to its incurable long-lasting nature and prevalence of physical and psychological incapacities. In this study, an attempt was made to evaluate the prevalence of G6PD deficiency among the Saudi population in Riyadh city. A cross-sectional retrospective study was conducted at King Saud University Medical City in Riyadh, Saudi Arabia. The population of the study comprised randomly chosen males and females who visited the hospital from January 2017 to January 2018. Statistical analyses were performed using SPSS, and descriptive analysis was used to find the frequency of G6PD-deficient patients. Out of the 209 patients, 62.2% were males (n=130) and 37.8% were females (n=79). Twenty males and 6 females were found to have G6PD deficiency, with the male to female ratio being 1:3. Out of the total 130 male participants, 20 patients were found to be enzyme deficient and 6 patients of 79 female patients were found to be G6PD deficient. There were 38.4% (n=10) patients with G6PD level <4 units/gram hemoglobin, 26.9% (n=7) patients had G6PD levels of 4.1–7.0 units/gram hemoglobin, and 34.6% (n=9) patients had >7 units/gram hemoglobin. Among the G6PD patients, 23.07% patients were severely anemic, and 5 (19.2%) patients were reported to have high bilirubin. The present study revealed the G6PD prevalence to be 12.4% among the Saudi population; this value is significantly higher than that found in France, Spain, India, and Singapore. In the Saudi population, males are more vulnerable to G6PD-deficient than females. Hence, attention should be paid to G6PD-deficient patients while prescribing antimalarial medication. Such patients may be advised to avoid certain foods to minimize the risk of having hemolytic episodes.

scholarly journals Management of Brucellosis

1970 ◽  
Vol 3 (1) ◽  
pp. 16-20
Author(s):  
MA Samad Talukder ◽  
ARE Mohamed ◽  
MM Madkour ◽  
Thur Y Albaage
Keyword(s):  
Saudi Arabia ◽  
High Temperature ◽  
Diagnostic Criteria ◽  
Clinical Presentation ◽  
High Titre ◽  
Agglutination Test ◽  
Joint Involvement ◽  
Slow Response ◽  
Adequate Therapy

Experiences with management of 140 (82 males and 58 females) patients of brucellosis is presented. The diagnostic criteria was based on clinical presentation (fever, joint pains) and high titre positive brucella agglutination test. Forty-four (31.4%) patients responded in 2-9 days as evidenced by high temperature coming to normal on receipt of adequate therapy by tetracycline alone or in combination with streptomycin or other drugs. Inappropriate antibiotic in inappropriate doses was given to 23 (16.4%) patients resulting in a slow response. More than half 73 (52%) were not treated as they did not attend for follow up for result of investigation or misdiagnosis. The complications were bone and joint involvement and endocarditis. To give adequate therapy and avoid complications any patient with pyrexia in Saudi Arabia should be investigated for brucellosis.DOI: http://dx.doi.org/10.3329/bjms.v3i1.8222BJMS 1996; 3(1): 16-20

The incidence and clinical manifestations of human brucellosis in a referral hospital in Southern Saudi Arabia between 2015 and 2019

2021 ◽  
Author(s):  
Mutasim E. Ibrahim ◽  
Mohammad S. Al-Shahrani
Keyword(s):  
Saudi Arabia ◽  
Joint Pain ◽  
Clinical Manifestations ◽  
Signs And Symptoms ◽  
Referral Hospital ◽  
Human Brucellosis ◽  
Local Health Authority ◽  
Local Health ◽  
Laboratory Findings ◽  
Body Ache

AbstractWe determined the incidence and clinical manifestations of human brucellosis from patients who attended a referral hospital in South of Saudi Arabia. A record-based retrospective study was conducted from January 2015 to December 2019 at King Abdulla Hospital, Bisha, Saudi Arabia. Information on patients’ demographic characteristics, detailed records of signs and symptoms, and the laboratory findings were reviewed. Of 6,586 patients included, 15.8% (n = 1,041) were infected with brucellosis. The age of infected individuals ranged from five to 95 years, with an average of 35.1 ± 21.2 years. Most infected patients were male (72.3%). Young adults (26–44 years) were the most common age group with the disease (34.1%). The annual rate of infection significantly decreased (P < 0.0001) from 33.2% in 2015 to 12.5% in 2019. An escalating number of brucellosis cases was seen in the spring and peaked during the summer. Fever (35.3%), joint pain (25.5%), generalized body ache (10.7%), and neurological symptoms (10.0%) were the most frequent clinical manifestation associated with brucellosis. Joint pain was commonly found among children (44.4%). Neurological findings were more frequent among adult patients. The study concluded that brucellosis is endemic in Southern Saudi Arabia and needs local health authority to implement preventive and educational program measures. Infected patients may present with diverse, nonspecific clinical manifestations that require intuition from clinicians to detect the disease.

scholarly journals Seroprevalence of human Brucellosis and associated risk factors among high-risk occupations in Mbeya Region of Tanzania

2020 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Frederick D. Sagamiko ◽  
Ruth L. Mfune ◽  
Bernard M. Hang’ombe ◽  
Esron D. Karimuribo ◽  
Alfred M. Mwanza ◽  
...  
Keyword(s):  
Public Health ◽  
Risk Factors ◽  
Statistical Significance ◽  
Raw Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Brucellosis ◽  
P Value ◽  
Cross Sectional ◽  
Occupationally Exposed ◽  
Associated Risk Factors

Background: Brucellosis is a bacterial zoonotic disease of public health and economic importance. A cross-sectional study was conducted in Mbeya region between November 2015 and January 2016 to investigate the seroprevalence of human brucellosis and identify associated risk factors among individuals in risky occupations. Methods: A total of 425 humans from six occupational categories were serially tested for Brucella antibodies using the Rose Bengal Plate Test (RBPT) and competitive Enzyme Linked Immunosorbent Assay (c-ELISA), for screening and confirmation, respectively. A questionnaire survey was also administered to participants to collect epidemiological data. Results: The overall seroprevalence among the occupationally exposed individuals was 1.41% (95% CI: 00.64-3.12). Seroprevalence was higher among butcher men 5.6% (95% CI: 1.68-5.26), herds men 1.35% (95% CI: 0.18-9.02); and abattoir workers 1.1% (95% CI: 0.26-4.22) although there was no statistical significance. (P value = .18). Seroprevalence was also higher among men (1.8%) compared to females (0%) (P value = .19). and also, among those aged < 11 years (2.5%). Individuals who consumed raw milk had a higher seroprevalence (1.56%) compared to those who drunk boiled milk while seropositivity was 0.88% among those who assisted animals during parturition (P value = .49). Butcher men were at higher risk of exposure compared to other occupational categories. Our findings show the presence of brucellosis in occupationally exposed individuals in Mbeya region.Conclusion: There is need to sensitize the concerned professions in order to reduce the risk of acquiring Brucella infections from animals and animal products This also calls for public health awareness about the disease, and implementation of measures to prevent further spread of brucellosis within and outside the study area.

Sign in / Sign up

Export Citation Format

Share Document