scholarly journals Antibacterial and antifungal activities of the ethanol extract of some medicinal plants

2020 ◽  
Vol 3 (4) ◽  
pp. 43-51
Author(s):  
Ngoc Nguyen Nhu ◽  
Minh Vu Thi Nguyet ◽  
Tuyen Phung Thi ◽  
Huong Lan Nguyen Thi ◽  
◽  
...  
Keyword(s):  
Curcuma Longa ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Piper Betle ◽  
Antibacterial And Antifungal Activities ◽  
Agar Disc ◽  
Phytochemical Compounds ◽  
Ethanol Extracts ◽  
Antibacterial And Antifungal

The aim of this study was to identify and evaluate for antimicrobial activity and phytochemical properties of the extracts from some plants belonging to different species such as Piper betle L., Allium cepa L., Allium ascalonium L., Allium Sativum L., Curcuma longa L.; Paederia tomentosa L.; Wedelia chinensis M. and Chromolaena odorata L. These extracts have been prepared and the antimicrobial activities have been examined on some bacteria strains like Escherichia coli T1, Bacillus cereus M1; Salmonella sp. ST; Shigella sp. AT and one fungus strain Aspegillus flavus KN by agar disc diffusion method. Results showed that the extracts from total of 8 plant species have been inhibited the growth of all over the micro-organisms strains used in the test at a different ratio, but the ethanol extracts from Piper betle and Wedelia chinensis M. showed the highest effect. Also, the extracts then were tested for the presence of bioactive compounds. The results showed that there were tannins, flavones, carbohydrates, proteins, and oils in Wedelia chinensis M.extracts. In Piper betle extracts there were many phytochemical compounds including phenols, tannins, saponins, carbohydrates, proteins, resins, and oils.

Comparative study of the ethanol extracts of six Acinos Miller species: chemical composition, antimicrobial and antioxidative activities

2020 ◽  
Vol 48 (1) ◽  
pp. 53-65
Author(s):  
Tatjana D. GOLUBOVIĆ ◽  
Gordana S. STOJANOVIĆ ◽  
Dušanka V. KITIĆ ◽  
Bojan K. ZLATKOVIĆ ◽  
Dragana R. PAVLOVIĆ ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Salmonella Enteritidis ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Fungal Organisms ◽  
Ethanol Extracts

The ethanol extracts of selected Acinos Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of A. majoranifolius, A. suaveolens and A. alpinus were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the A. arvensis and A. graveolens extracts reached 28.97% and 30.75%. Also, n-alkanes were the major compounds found in A. hungaricus ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus; Gram-negative: Klebsiella pneumoniae, Pseudomonas aerugionsa, Salmonella enteritidis, Proteus vulgaris, Escherichia coli and fungal organisms: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae were used as test microorganisms. The results of preliminary bioassay demonstrated that the A. alpinus ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.

scholarly journals In VitroPhytochemical, Antibacterial, and Antifungal Activities of Leaf, Stem, and Root Extracts ofAdiantum capillus veneris

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Muhammad Saqib Ishaq ◽  
Muhammad Medrar Hussain ◽  
Muhammad Siddique Afridi ◽  
Ghadir Ali ◽  
Mahrukh Khattak ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Antifungal Activities ◽  
Fungal Strains ◽  
Antibacterial And Antifungal Activities ◽  
Adiantum Capillus Veneris ◽  
Ethanol Extracts ◽  
Antibacterial And Antifungal

Adiantum capillus venerisis a medicinally essential plant used for the treatment of diverse infectious diseases. The study of phytochemical and antimicrobial activities of the plant extracts against multidrug-resistant (MDR) bacteria and medically important fungi is of immense significance. Extracts from the leaves, stems, and roots ofAdiantum capillus veneriswere extracted with water, methanol, ethanol, ethyl acetate, and hexane and screened for their antimicrobial activity against ten MDR bacterial strains and five fungal strains isolated from clinical and water samples. Ash, moisture, and extractive values were determined according to standard protocols. FTIR (Fourier transform infrared Spectroscopy) studies were performed on different phytochemicals isolated from the extracts ofAdiantum capillus Veneris. Phytochemical analysis showed the presence of flavonoids, alkaloids, tannins, saponins, cardiac glycosides, terpenoids, steroids, and reducing sugars. Water, methanol, and ethanol extracts of leaves, stems, and roots showed significant antibacterial and antifungal activities against most of the MDR bacterial and fungal strains. This study concluded that extracts ofAdiantum capillus venerishave valuable phytochemicals and significant activities against most of the MDR bacterial strains and medically important fungal strains.

scholarly journals Synthesis, Characterization, and Biological Studies of Binuclear Copper(II) Complexes of (2E)-2-(2-Hydroxy-3-Methoxybenzylidene)-4N-Substituted Hydrazinecarbothioamides

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
P. Murali Krishna ◽  
B. S. Shankara ◽  
N. Shashidhar Reddy
Keyword(s):  
Spectroscopic Characterization ◽  
Azomethine Nitrogen ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Binuclear Copper ◽  
Antibacterial And Antifungal Activities ◽  
Agar Disc ◽  
Biological Studies ◽  
Antibacterial And Antifungal ◽  
Ct Dna

Four novel binuclear copper(II) complexes [1–4] of (2E)-2-(2-hydroxy-3-methoxybenzylidene)-4N-substituted hydrazinecarbothioamides, (OH)(OCH3)C6H4CH=NNHC(S)NHR, where R = H (L1), Me (L2), Et (L3), or Ph (L4), have been synthesized and characterized. The FT-IR spectral data suggested the attachment of copper(II) ion to ligand moiety through the azomethine nitrogen, thioketonic sulphur, and phenolic-O. The spectroscopic characterization indicates the dissociation of dimeric complex into mononuclear [Cu(L)Cl] units in polar solvents like DMSO, where L is monoanionic thiosemicarbazone. The DNA binding properties of the complexes with calf thymus (CT) DNA were studied by spectroscopic titration. The complexes show binding affinity to CT DNA with binding constant (Kb) values in the order of 106 M−1. The ligands and their metal complexes were tested for antibacterial and antifungal activities by agar disc diffusion method. Except for complex 4, all complexes showed considerable activity almost equal to the activity of ciprofloxacin. These complexes did not show any effect on Gram-negative bacteria, whereas they showed moderate activity for Gram-positive strains.

An Invitro Study: Antioxidant, Phytochemical Analysis and Antimicrobial Activities of Achranthes aspera

2021 ◽  
pp. 268-277
Author(s):  
Masiraben Vahora ◽  
Falaknaaz Shaikh
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Antibacterial And Antifungal Activities ◽  
Fusarium Sp ◽  
Antibacterial And Antifungal

Medicaments, plants and plant-based are the basis of many of the modern pharmaceuticals we use today for our various purposes. The aim of the present study was to evaluate the antioxidant, phytochemical and antibacterial and antifungal activities of the Achyranthes aspera plant extract in different organic solvents. The radical scavenging activity of the different extracts of root, stem, leaf, and seed were evaluated by DPPH assay and the antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa, and antifungal activity against Fusarium sp. and Aspergillus niger was studied by Agar well cut diffusion method. All of the extracts exhibited different antioxidant and antibacterial activities and the activities varied from solvent to solvent, and the activities are concentrated. The antioxidant and antimicrobial activities were compared with the positive control Ascorbic acid and Cefuroxime. A qualitative phytochemical analysis was carried out and found to possess bioactive compounds like alkaloids, glycosides, terpenoids, steroids, flavonoids, tannins.

scholarly journals Total phenolic content, antioxidant, antibacterial and antifungal activities, FT-IR analyses of Brassica oleracea L. var. acephala and Ornithogalum umbellatum L.

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 229-244 ◽  
Author(s):  
Sinem Aydin
Keyword(s):  
Antioxidant Activity ◽  
Brassica Oleracea ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Ft Ir ◽  
Ethanol Extracts ◽  
Antibacterial And Antifungal

Brassica oleracea var. acephala and Ornithogalum umbellatum are two plant species which belong Brassicaceae and Liliaceae, respectively. Both of them are used as food and medicinally. O. umbellatum is used as anticancer, diuretic anti anti emetic. B. oleracea var. acephala is used for gastritis, gastric and duodenal ulcers and preventing some cancer forms such as stomach cancer, breast cancer and lung cancer. The aim of this research was to search antioxidant, antibacterial and antifungal activities and FT-IR analyses of ethanol extracts of stem and flower parts of B. oleracea var. acephala and O. umbellatum. Extracts were obtained by Soxhlet extraction. Ethanol extracts of B. oleracea var. acephala showed higher antibacterial activity than ethanol extracts of O. umbellatum. The most effective antifungal extract was ethanol extract of flower part of O. umbellatum against the fungi Candida. tropicalis and Candida. parapsilosis. Antioxidant activity of the extracts were also studied. The highest total phenolic and flavonoid contents were found in flower part of B. oleracea var. acephala as 50.7?0.007 ?g GAE/mL and stem part of O. umbellatum as 81.37?0.006 ?g CE/mL, respectively. BHT which used as standard antioxidant had higher total antioxidant activity than tested ethanol extracts. DPPH activity of the extracts increased in the following order: Flower part of B. oleracea var. acephala> Stem part of O. umbellatum> Flower part of O. umbellatum> Stem part of B. oleracea var. acephala. These results which obtained from this study demonstrate that B. oleracea var. acephala and O. umbellatum species might be utilized as an alternative to synthetic antioxidans and antimicrobials.

scholarly journals Gas Chromatographic Analysis of the Phyto-Constituents and the Assessment of the Anti-Microbial Properties of the Leave Extracts of Nigeria-Grown Gingko biloba

2020 ◽  
Vol 1 (2) ◽  
pp. 45-56
Author(s):  
Kingsley Ubaoji ◽  
Onyeka Nwosu ◽  
Kingsley Agu ◽  
Kingsley Nwozor ◽  
Nma Ifedilichukwu ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Good Health ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Therapeutic Effects ◽  
Gingko Biloba ◽  
Antibacterial And Antifungal Activities ◽  
Zones Of Inhibition ◽  
Pharmaceutical Industries ◽  
Ethanol Extracts

Purpose: Gingko biloba (GB) leaves have been recognized for their therapeutic effects, dating back to traditional Chinese medicine where the ground leaves were used to treat several health problems. GB extracts are renowned for their medicinal properties in Asia, Europe and South America. However, GB leaves are rarely grown and used in Nigeria. In this study, we screened the phytochemical constituents and antimicrobial activities of GB leaves (aqueous and ethanol extracts) grown in Enugu, Enugu State, South East Nigeria. Study Design: Experimental Materials and Methods: Quantitative analyses of phytochemicals were done using gas chromatography while disk diffusion and broth dilution methods were used to determine the antimicrobial activities. Results: The phytochemical analysis of aqueous and ethanol extracts showed high quantity of flavonoids (anthocyanin, rutin, epicatechin, kaemferol and catechin), alkaloids (lunamarine and ribalindine) and saponin while the moderate quantity of steroids and terpenes, tannin and phenols were also observed. The phytochemical analysis also showed very little concentration of antinutrients (phytate and oxalate) in both extracts. The ethanol extract showed some degree of antibacterial and antifungal activities as observed from their zones of inhibition (mm). Activity was observed on gram-positive bacteria; Staphylococcusaureus (15.5+0.71mm) and less activity on gram-negative bacteria; Escherichia coli (10.5+1.41mm) and Pseudomonasaeruginosa (no activity). Also there was an activity on yeast; Candida albicans (16.5+0.71mm) and less activity on moulds; Penicilliumcyclopium (9+1.41mm) and Aspergillus fumigatus (no activity). The aqueous extract showed no antimicrobial activity on both bacteria and fungi. Conclusion: This study revealed the medicinal potentials of Ginkgo biloba leaves grown in Nigeria. The findings, therefore, will be useful for pharmaceutical industries and medical practice in Nigeria and beyond for the provision of good health to individuals.

scholarly journals Synthesis of disulphide-Schiff base derivatives and investigations of in vitro antimicrobial activities against some human pathogens

2017 ◽  
Vol 1 (3) ◽  
pp. 230-234 ◽  
Author(s):  
Sefa Durmuş ◽  
Aslıhan Dalmaz ◽  
Görkem Dülger ◽  
Duygu Bircan Kadıoğlu
Keyword(s):  
Schiff Base ◽  
Schiff Bases ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Control Group ◽  
Human Pathogens ◽  
Antibacterial And Antifungal Activities ◽  
Schiff Base Derivatives ◽  
Antibacterial And Antifungal

Abstract Thio-Schiff bases are becoming increasingly widespread in various branches such as the preparation of certain medicines, cosmetic products, and polymer production. In particular, the presence of antibacterial, antifungal, antiviral, antitumor and antimalarial properties of Schiff bases containing sulfur in the structure has made these compounds attractive in different disciplines. In this study, different derivatives of dimeric disulfide-Schiff bases have been synthesized. The antibacterial and antifungal activities of the synthesized these compounds were investigated in vitro against some human pathogens (Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Candida albicans, C. tropicalis, C. guilliermondii and C. glabrata). Test microorganisms were isolated from the patients appyling to Medical Faculty Hospital of Duzce University were used. Diffusion method was used to determine the antimicrobial activities of the compounds.standard antibacterial (Cefotaxime, Amoxicillin/clavulanicacid) and antifungal (Posaconazole) antibiotics were used as the control group and the results were compared. The result indicated that antimicrobial activity of Disulphide-Schiff Base Derivatives exhibited less activity against bacteria as compared to AMC30 (Amoxicillin/clavulanicacid), but highly effective against bacteria as compared to CTX30 (Cefotaxime). In addition, the compounds exhibited less activity against yeast.

Antibacterial Activity of Ethanol Extract from Stem Bark and Leaves of ­Berenuk (Crescentia cujete L.)

2017 ◽  
Vol 4 (1) ◽  
pp. 1-14
Author(s):  
Uswatun Hasanah ◽  
Desi Rosdiana ◽  
Syaefudin Syaefudin
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Diffusion Method ◽  
Contact Method ◽  
E Coli ◽  
Antibacterial Assay ◽  
Phytochemical Compounds ◽  
Crescentia Cujete ◽  
Ethanol Extracts

Crescentia cujete L. (C. cujete) has been known as a medicine for various diseases that caused by microorganisms. This research was aimed to identify the phytochemical compounds and to determine the antibacterial activity of ethanolic extracts from stem bark and leaves of C. cujete. The phytochemical compounds in both extracts were identified by Harborne method, while antibacterial activity assay was performed by disc diffusion method with the concentration of bacteria 106 cfu/mL. Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) were used in antibacterial assay. Minimum Inhibitory Concentration (MIC) was obtained by using contact method. The phytochemical compounds analysis showed that ethanol extracts of C. cujete stem bark and leaves contained alkaloids, flavonoids, saponins, tannins, and steroids. The results of antibacterial activity test showed that both extracts have antibacterial activity with the highest inhibition showed by extracts with concentration of 100 %(w/v). The decreased percentage in the number of bacterial colonies on the extracts was less than 90 % so that the MIC value of both extracts against S. aureus could not be determined.

scholarly journals Short Communication: Evaluation of antimicrobial activities of Alchemilla vulgaris and Portulaca oleracea ethanolic extracts and correlation with their phytochemical profiles

2017 ◽  
Vol 15 (2) ◽  
pp. 96-99
Author(s):  
SALEM MOHAMED EDRAH
Keyword(s):  
Antimicrobial Activity ◽  
Short Communication ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Portulaca Oleracea ◽  
Diffusion Method ◽  
Ethanolic Extracts ◽  
Communication Evaluation ◽  
Ethanol Extracts ◽  
Phytochemical Profiles

Edrah SM. 2017. Short Communication: Evaluation of antimicrobial activities of Alchemilla vulgaris and Portulaca oleracea ethanolic extracts and correlation with their phytochemical profiles. Biofarmasi J Nat Prod Biochem 15: 96-99. The ethanol extracts of leaves of Alchemilla vulgaris and Portulaca oleracea were studied for antimicrobial activity at 10 mg/mL concentrations by using disc diffusion method on two gram-positive bacteria: Staphylococcus aureus and Staphylococcus epidermidis; three gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa and one fungi: Candida albicans were used in the study. After incubation for 24 hrs, the zone of inhibition was compared with standard antibiotics Gentamycin (10 μg/disc) which was used as positive control. From the dose-dependent study, it was concluded that the ethanol extract of A. vulgaris was more potential than leave extract of P. oleracea. Almost all of the chemical ingredients present in both ethanol extracts such as tannins, flavonoids, phenols which may be responsible for the antimicrobial activity.

scholarly journals Synthesis and Evaluation of Antimicrobial Activities of Novel N-Substituted Indole Derivatives

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Tanveer MahamadAlli Shaikh ◽  
Habtamu Debebe
Keyword(s):  
Antimicrobial Activities ◽  
New Drugs ◽  
Diffusion Method ◽  
Indole Derivatives ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Standard Drug ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal

Indole motifs are one of the most significant scaffolds in the discovery of new drugs. We have described a synthesis of new N-substituted indole derivatives (1-3), and their in vitro antimicrobial activities were investigated. The synthesis of titled compounds has been demonstrated by utilizing commercially available starting materials. The antibacterial and antifungal activities were performed using new strains of bacteria Staphylococcus aureus, Escherichia coli, and Candida albicans using the disc diffusion method. Notably, the compound 4-(1-(2-(1H-indol-1-yl) ethoxy) pentyl)-N,N-dimethyl aniline (1) was found to be most potent than the other analogues (2 and 3), which has shown higher inhibition than the standard drug chloramphenicol.

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