scholarly journals Effect of Various Calcite Precipitating Bacteria on Compressive Strength of Concrete: A Comparative Study

2021 ◽  
pp. 158-168
Keyword(s):  
Escherichia Coli ◽  
Compressive Strength ◽  
Cell Concentration ◽  
Culture Media ◽  
Calcium Lactate ◽  
Fine Aggregate ◽  
E Coli ◽  
Compressive Strength Of Concrete ◽  
Maximum Compressive Strength ◽  
Mixing Water

The presence of micro pores in concrete makes the concrete weak and less durable, in this study an ecofriendly attempt has been made to minimize these micro pores by calcite precipitating bacteria i.e. Escherichia Coli, bacillus subtilisand pseudomonas aeruginosa which produce calcium carbonate when they blend with calcium lactate in presence of water and air thus filling up the micro pores, thus comparing their effects on compressive strength of concrete.Various proportions of E. coli, B.subtilisand P. aeruginosa bacterial media were impregnated in concrete viz. 10%,20% and 30% replacing the proportion of mixing water, small proportion of fine aggregate was also replaced by calcium lactate and silica gel. It was observed that concrete with 10% replacement of P. aeruginosa culture media with mixing water showed maximum compressive strength with 79.6% increase in the compressive strength of concrete where as concrete with B.subtilisbacterial media showed 61.1% increase in the compressive strength of concrete and concrete with E.coli bacterial media showed 23.5% increase in the compressive strength of the concrete, keeping 10-6 cells/ml the cell concentration for all bacterial concretes.

The Influence of Glycosylation on the Catalytic and Fibrinolytic Properties of Pro-Urokinase

1992 ◽  
Vol 68 (05) ◽  
pp. 539-544 ◽  
Author(s):  
Catherine Lenich ◽  
Ralph Pannell ◽  
Jack Henkin ◽  
Victor Gurewich
Keyword(s):  
Escherichia Coli ◽  
Mammalian Cell ◽  
Human Gene ◽  
Culture Media ◽  
Mammalian Cell Culture ◽  
Glycosylation Site ◽  
Clot Lysis ◽  
Cell Culture Media ◽  
E Coli ◽  
The Uk

SummaryWe previously found that human pro-UK expressed in Escherichia coli is more active in fibrinolysis than recombinant human pro-UK obtained from mammalian cell culture media. To determine whether this difference is related to the lack of glycosylation of the E. coli product, we compared the activity of E. coli-derived pro-UK [(-)pro-UK] with that of a glycosylated pro-UK [(+)pro-UK] and of a mutant of pro-UK missing the glycosylation site at Asn-302 [(-) (302) pro-UK]. The latter two pro-UKs were obtained by expression of the human gene in a mammalian cell. The nonglycosylated pro-UKs were activated by plasmin more efficiently (≈2-fold) and were more active in clot lysis (1.5-fold) than the (+)pro-UK. Similarly, the nonglycosylated two-chain derivatives (UKs) were more active against plasminogen and were more rapidly inactivated by plasma inhibitors than the (+)UK.These findings indicate that glycosylation at Asn-302 influences the activity of pro-UK/UK and could be the major factor responsible for the enhanced activity of E. coli-derived pro-UK.

Experimental Study on Sand and Cement Replacement by Termite Mound Soil

2019 ◽  
pp. 279-287
Author(s):  
Harish R ◽  
Ramesh S ◽  
Tharani A ◽  
Mageshkumar P
Keyword(s):  
Experimental Study ◽  
Experimental Investigation ◽  
Compressive Strength ◽  
Natural Environment ◽  
Fine Aggregate ◽  
Test Results ◽  
Termite Mound ◽  
Cement Concrete ◽  
Cement Ratio ◽  
Compressive Strength Of Concrete

This paper presents the results of an experimental investigation of the compressive strength of concrete cubes containing termite mound soil. The specimens were cast using M20 grade of concrete. Two mix ratios for replacement of sand and cement are of 1:1.7:2.7 and 1:1.5:2.5 (cement: sand: aggregate) with water- cement ratio of 0.45 and varying combination of termite mound soil in equal amount ranging from 30% and 40% replacing fine aggregate (sand) and cement from 10%,15%,20% were used. A total of 27 cubes, 18 cylinders and 6 beams were cast by replacing fine aggregate, specimens were cured in water for 7,14 and 28 days. The test results showed that the compressive strength of the concrete cubes increases with age and decreases with increasing percentage replacement of cement and increases with increasing the replacement of sand with termite mound soil cured in water. The study concluded that termite mound cement concrete is adequate to use for construction purposes in natural environment.

scholarly journals Utilization of tailings in cement and concrete: A review

2019 ◽  
Vol 26 (1) ◽  
pp. 449-464 ◽  
Author(s):  
Mifeng Gou ◽  
Longfei Zhou ◽  
Nathalene Wei Ying Then
Keyword(s):  
Compressive Strength ◽  
Cementitious Materials ◽  
Solid Wastes ◽  
Supplementary Cementitious Materials ◽  
Cement Clinker ◽  
Fine Aggregate ◽  
Granulated Blast Furnace Slag ◽  
Fine Aggregates ◽  
Compressive Strength Of Concrete ◽  
Cement And Concrete

AbstractOne of the advantages of cement and the cement concrete industry in sustainability is the ability to utilize large amounts of industrial solid wastes such as fly ash and ground granulated blast furnace slag. Tailings are solid wastes of the ore beneficiation process in the extractive industry and are available in huge amounts in some countries. This paper reviews the potential utilization of tailings as a replacement for fine aggregates, as supplementary cementitious materials (SCMs) in mortar or concrete, and in the production of cement clinker. It was shown in previous research that while tailings had been used as a replacement for both fine aggregate and cement, the workability of mortar or concrete reduced. Also, at a constant water to cement ratio, the compressive strength of concrete increased with the tailings as fine aggregate. However, the compressive strength of concrete decreased as the replacement content of the tailings as SCMs increased, even whentailings were ground into smaller particles. Not much research has been dedicated to the durability of concrete with tailings, but it is beneficial for heavy metals in tailings to stabilize/solidify in concrete. The clinker can be produced by using the tailings, even if the tailings have a low SiO2 content. As a result, the utilization of tailings in cement and concrete will be good for the environment both in the solid waste processing and virgin materials using in the construction industry.

scholarly journals Evaluation of the Antibacterial Activity of a Geopolymer Mortar Based on Metakaolin Supplemented with TiO2 and CuO Particles Using Glass Waste as Fine Aggregate

Coatings ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 157 ◽  
Author(s):  
Ruby Gutiérrez ◽  
Mónica Villaquirán-Caicedo ◽  
Sandra Ramírez-Benavides ◽  
Myriam Astudillo ◽  
Daniel Mejía
Keyword(s):  
Culture Media ◽  
Raw Materials ◽  
Silicate Solution ◽  
Fine Aggregate ◽  
X Ray Diffraction ◽  
Glass Waste ◽  
Homogeneous Surface ◽  
E Coli ◽  
Study Results ◽  
Antibacterial Surfaces

Metakaolin-based geopolymer cements were produced by alkaline activation with a potassium hydroxide and potassium silicate solution. To produce the geopolymer composites, 10 wt.% titanium oxide (TiO2) and 5 wt.% copper oxide (CuO) nanoparticles were used. The geopolymer mortar was prepared using glass waste as fine aggregate. The raw materials and materials produced were characterized by X-ray diffraction, electron microscopy, and Fourier-transform infrared spectroscopy techniques. Likewise, the geopolymer samples were characterized to determine their physical properties, including their density, porosity, and absorption. The photocatalytic activity of the materials was evaluated by activating the nanoparticles in a chamber with UV–Vis light during 24 h; then, different tests were performed to determine the growth inhibition of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa bacteria in nutrient agar for times of up to 24 h. The study results showed that a geopolymer mortar containing glass waste as fine aggregate (GP-G) exhibited a water absorption 56.73% lower than that of the reference geopolymer paste without glass (GP). Likewise, glass particles allowed the material to have a smoother and more homogeneous surface. The pore volume and density of the GP-G were 37.97% lower and 40.36% higher, respectively, than those of the GP. The study with bacteria showed that, after 24 h in the culture media, the GP-G mortars exhibited a high inhibition capacity for the growth of P. aeruginosa from solutions of 10−4 mL and in solutions of 10−6 mL for E. coli and S. aureus. These results indicate the possibility of generating antibacterial surfaces by applying geopolymer composite.

Survival of Escherichia coli O157:H7 and Campylobacter jejuni in Bottled Purified Drinking Water under Different Storage Conditions

2011 ◽  
Vol 74 (2) ◽  
pp. 254-260 ◽  
Author(s):  
HAMZAH M. AL-QADIRI ◽  
XIAONAN LU ◽  
NIVIN I. AL-ALAMI ◽  
BARBARA A. RASCO
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Campylobacter Jejuni ◽  
Culture Media ◽  
False Negative ◽  
Storage Conditions ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Log Reduction ◽  
Negative Results

Survival of Escherichia coli O157:H7 and Campylobacter jejuni that were separately inoculated into bottled purified drinking water was investigated during storage at 22, 4, and −18°C for 5, 7, and 2 days, respectively. Two inoculation levels were used, 1 and 10 CFU/ml (102 and 103 CFU/100 ml). In samples inoculated with 102 CFU/100 ml, C. jejuni was not detectable (>2-log reduction) after storage under the conditions specified above. E. coli O157:H7 was detected on nonselective and selective media at log reductions of 1.08 to 1.25 after storage at 22°C, 1.19 to 1.56 after storage at 4°C, and 1.54 to 1.98 after storage at −18°C. When the higher inoculation level of 103 CFU/100 ml was used, C. jejuni was able to survive at 22 and 4°C, with 2.25- and 2.17-log reductions, respectively, observed on nonselective media. At these higher inoculation levels, E. coli O157:H7 was detectable at 22, 4, and −18°C, with log reductions of 0.76, 0.97, and 1.21, respectively, achieved on nonselective media. Additionally, E. coli O157:H7 showed significant differences in culturability (P < 0.05) on the nonselective and selective culture media under the different storage conditions, with storage at −18°C for 2 days being the treatment most inhibiting. The percentage of sublethal injury of E. coli O157:H7 ranged from ~33 to 75%, indicating that microbial examination of bottled water must be done carefully, otherwise false-negative results or underestimation of bacterial numbers could pose a health risk when low levels of pathogens are present.

scholarly journals Specific Secretion of Active Single-Chain Fv Antibodies into the Supernatants of Escherichia coliCultures by Use of the Hemolysin System

2000 ◽  
Vol 66 (11) ◽  
pp. 5024-5029 ◽  
Author(s):  
Luis A. Fernández ◽  
Isabel Sola ◽  
Luis Enjuanes ◽  
Víctor de Lorenzo
Keyword(s):  
Escherichia Coli ◽  
Transport System ◽  
Culture Media ◽  
Single Chain ◽  
Extracellular Medium ◽  
Simple Method ◽  
E Coli ◽  
Single Chain Fv ◽  
Bacterial Cytoplasm ◽  
Culture Supernatants

ABSTRACT A simple method for the nontoxic, specific, and efficient secretion of active single-chain Fv antibodies (scFvs) into the supernatants ofEscherichia coli cultures is reported. The method is based on the well-characterized hemolysin transport system (Hly) of E. coli that specifically secretes the target protein from the bacterial cytoplasm into the extracellular medium without a periplasmic intermediate. The culture media that accumulate these Hly-secreted scFv's can be used in a variety of immunoassays without purification. In addition, these culture supernatants are stable over long periods of time and can be handled basically as immune sera.

Persistent bloody diarrhoea without fever associated with diffusely adherent Escherichia coli in a young child

2013 ◽  
Vol 62 (12) ◽  
pp. 1907-1910 ◽  
Author(s):  
Sandra Patzi-Vargas ◽  
Mussaret Zaidi ◽  
Rodolfo Bernal-Reynaga ◽  
Magda León-Cen ◽  
Alba Michel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Culture Media ◽  
Evidence Base ◽  
Antimicrobial Treatment ◽  
Molecular Techniques ◽  
Bloody Diarrhoea ◽  
Severe Diarrhoea ◽  
E Coli ◽  
Clinical Laboratories

Diffusely adherent Escherichia coli (DAEC) is thought to cause diarrhoea in children, and so too are other diarrhoeagenic E. coli (DEC); however, the evidence base is inconclusive. DEC pathotypes are differentiated on the basis of their pathogenic features, and thus cannot be quickly identified on selective culture media. Molecular techniques, not readily available in most clinical laboratories, are required to differentiate DEC strains from non-pathogenic E. coli in the stool flora. We report a case of persistent bloody diarrhoea, without fever, in a previously healthy 21-month infant from whom we isolated five DAEC strains. The child’s stools movements were loose, with gross blood and mucus; fresh mount analysis revealed numerous faecal leukocytes and erythrocytes. Response to antimicrobial treatment with trimethoprim-sulfamethoxazole was poor despite susceptibility in vitro. Although the patient improved with azithromycin, blood was present in the patient’s stools for over 30 days. The severe diarrhoea in this patient might be explained by the fact that these DAEC isolates harboured a siderophore receptor, which allows the bacteria to use iron derived from haem compounds that promote its multiplication. The isolates also induced in vitro secretion of several immunomodulatory cytokines that may account for the patient’s loose stools and faecal leukocytes. DAEC may play a greater role than suspected in afebrile children with bloody diarrhoea.

scholarly journals THE EFFECT OF ADDING CEMENT WASTE ON THE QUALITY OF CONCRETE COMPRESSIVE

Jurnal CIVILA ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 213
Author(s):  
Asrul Majid ◽  
Hammam Rofiqi Agustapraja
Keyword(s):  
Compressive Strength ◽  
Strength Test ◽  
Fine Aggregate ◽  
Infrastructure Development ◽  
Test Results ◽  
Compressive Strength Test ◽  
Compressive Strength Of Concrete ◽  
Concrete Materials ◽  
Test Objects

Infrastructure development is one of the important aspects of the progress of a country where most of the constituents of infrastructure are concrete. The most important constituent of concrete is cement because its function is to bind other concrete materials so that it can form a hard mass. The large number of developments using cement as a building material will leave quite a lot of cement bags.In this study, the authors conducted research on the effect of adding cement waste to the compressive strength of concrete. This study used an experimental method with a total of 24 test objects. The test object is in the form of a concrete cylinder with a diameter of 15 cm and a height of 30 cm and uses variations in the composition of the addition of cement waste cement as a substitute for fine aggregate, namely 0%, 2%, 4% and 6%. K200). The compressive strength test was carried out at the age of 7 days and 28 days.The test results show that the use of waste as a partial substitute for fine aggregate results in a decrease in the compressive strength of each mixture. at the age of 7 days the variation of 2% is 16.84 MPa, 4% is 11.32 MPa and for a mixture of 6% is 6.68 MPa. Meanwhile, the compressive strength test value of 28 days old concrete in each mixture decreased by ± 6 MPa. So the conclusion is cement cement waste cannot be used as a substitute for fine aggregate in fc 16.6 (K200) quality concrete because the value is lower than the specified minimum of 16.6 MPa.

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