scholarly journals EFFECT OF OBESTATIN ON PITUITARY GONADAL AXIS, LEPTIN AND MDA LEVELS IN OBESE SPRAGUE DAWLEY RATS

2021 ◽  
Vol 71 (3) ◽  
pp. 1061-65
Author(s):  
Nadia Latif ◽  
Amina Rasul ◽  
Sajid Ali ◽  
Rimsha Zia
Keyword(s):  
Obese Group ◽  
Control Group ◽  
Sprague Dawley ◽  
Group Iii ◽  
Army Medical ◽  
Testosterone Levels ◽  
Medical College ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Obese Rats

Objective: To determine the effect of obestatin administration on FSH, LH, testosterone, leptin and MDA levels in obese Sprague Dawley Rats. Study Design: Laboratory based animal study. Place and Duration of Study: Physiology department, Army Medical College Rawalpindi, from Mar to Jun 2015. Methodology: This randomized controlled trial was conducted at Physiology Department Army medical college. Male healthy Sprague Dawley rats were randomly divided into 3 groups (n–15 each) i.e. control group (group I) fed with normal pellet diet (NPD), obese group (group II) and obestatin treated obese group (group III) fed with high fat diet (HFD). After 10 weeks, group III was treated with obestatin (1nmol/100ml intraperitoneally). Blood samples were obtained by terminal intracardiac sampling for bioasssays of FSH, LH, testosterone, leptin and MDA by ELISA. Results: Obestatin supplementation in obese rats showed significant increase in LH levels (3.79 ± 0.05) and testosterone levels (2.07 ± 0.22) when compared to the non treated obese rats (2.19 ± 0.07) and (1.37 ± 0.15) respectively while significant decrease in leptin (3.85 ± 0.23) and MDA levels (1.62 ± 0.07) was observed when compared to the non-treated control groups (6.10 ± 1.18) and (1.95 ± 0.07) respectively. However, serum FSH levels remained unchanged among the treated and nontreated groups. Conclusion: Obestatin increases the testosterone levels by augmenting the pituitary gonadal axis through decrease in the oxidative stress and leptin levels in obese rats.

scholarly journals LIPID LOWERING BY STATIN;

2017 ◽  
Vol 24 (05) ◽  
pp. 745-750
Author(s):  
Faizania Shabbir ◽  
Mazhar Hussain ◽  
Shakir Khan ◽  
Tausif Ahmed Rajput
Keyword(s):  
Normal Control ◽  
Platelet Reactivity ◽  
Female Rats ◽  
Lipid Lowering ◽  
Sprague Dawley ◽  
Army Medical ◽  
Medical College ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Obese Rats

Objectives: To observe the effect of lipid lowering by atorvastatin administrationon platelet reactivity by measuring serum beta thromboglobulin concentration in male andfemale Sprague Dawley rats. Study Design: Randomized control trial (RCT). Place of study:The study was conducted at Physiology department, Army Medical College, Rawalpindi. Animalhandling, obesity induction, drug administration and sample collection were done at NationalInstitute of Health (NIH), Islamabad and biochemical assays were performed at Centre forResearch in Experimental and Applied Medicine (CREAM), Army Medical College, Rawalpindi.Period: 12 months. Material and Methods: Ninety healthy Sprague Dawley (male and female)rats were randomly selected and divided into three equal groups. Group I rats (normal control)were given normal chow diet for three weeks. Group II rats (obese control) were given high fatdiet for three weeks to induce obesity. Group III rats (obese treated) were given atorvastatinfor three weeks in a dose of 10 mg/kg/day orally by gavage method after obesity induction.Terminal blood sampling was done at the end of the study by intra-cardiac puncture. Bloodwas centrifuged to obtain serum and serum beta thromboglobulin was measured by usingEnzyme Linked Immunosorbant assay. Results: There was a significant (p < 0.05) increasein serum B-TG concentration in obese rats as compared to normal control rats. Atorvastatinadministration to obese rats significantly (p < 0.05) reduced serum B-TG concentration.Conclusions: Obesity increases and statin administration decreases platelet reactivity in highfat diet induced obese Sprague Dawley rats as reflected by serum B-TG concentration.

scholarly journals OBESITY

2015 ◽  
Vol 22 (06) ◽  
pp. 683-389
Author(s):  
Faizania Shabbir ◽  
M. Mazhar Hussain ◽  
Tausif Ahmed Rajput
Keyword(s):  
Platelet Count ◽  
Animal Models ◽  
Sprague Dawley ◽  
Immunosorbant Assay ◽  
Male And Female ◽  
Group Iii ◽  
Army Medical ◽  
Medical College ◽  
Group I ◽  
Wbc Count

Objective: To study the effects of atorvastatin administration on serum IL-6,WBC and platelet count in obese male and female animal models. Study Design: Randomizedcontrol trial (RCT). Place and duration of study: The study was conducted at Department ofPhysiology, Army Medical College, Rawalpindi in collaboration with National Institute of Health(NIH), Islamabad and Centre for Research in Experimental and Applied Medicine (CREAM),Army Medical College, Rawalpindi for funding, blood sampling and biochemical assaysrespectively. Material and Methods: Ninety healthy male and female Sprague Dawley ratswere selected and randomly divided into three equal groups. Group I rats were fed normal dietfor a period of three weeks. Group II rats were fed high fat diet for a period of three weeks toinduce obesity. Group III rats were administered atorvastatin 10 mg/kg/day orally by gavagemethod for three weeks after obesity induction. Terminal sampling by intra-cardiac puncturewas done at the end of study. Whole blood was used to perform blood complete picture by KX21 Sysmex Hematology Analyzer which includes platelet count and WBC count and serum wasused to measure IL-6 levels by Enzyme Linked Immunosorbant Assay (ELISA). Results: Therewas a significant decrease (p<0.05) in serum IL-6 levels and WBC count, whereas platelet countwas not significantly (p>0.05) affected by atorvastatin administration. Conclusions: Althoughatorvastatin reduces obesity related inflammation by decreasing serum IL-6 levels and WBCcount, it has no effect on platelet count in male and female obese animal models.

scholarly journals Psidium guajava leaves decrease arthritic symptoms in adjuvant-induced arthritic rats

2016 ◽  
Vol 34 (3) ◽  
pp. 197
Author(s):  
Hanif Nasiatul Baroroh ◽  
Esti Dyah Utami ◽  
Anisyah Achmad
Keyword(s):  
Knee Joint ◽  
Histopathological Examination ◽  
Chronic Phase ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Control Group ◽  
Sprague Dawley ◽  
Group Iii ◽  
Group I ◽  
Sprague Dawley Rats

BACKGROUND <br />Guava is an herbal with proven antioxidant and anti-inflammatory properties. The aim of this study was to investigate the anti-arthritic activity of the ethanol extract of Psidium gujava leaves (EEPG) against complete Freund’s adjuvant (CFA) induced arthritis in rats. <br /><br />METHODS<br />An experimental study was conducted on 40 male Wistar Sprague Dawley rats, which were divided into 5 groups. Each group was induced with 0.2 mL CFA (1 mg/mL) on day 1 and 0.1 CFA mL booster injection on day 5. Group I served as an arthritic control, group II received dexamethasone (6.75 mg.kg-1 orally), group III, IV and V received EEPG at oral doses of 250, 500, and 750 mg/kg BW, respectively, on days 14 to 28. Anti-arthritic activity was observed from the arthritis score, the paw circumference was measured on days 0, 1, 4, 8, 12, 16, 20, 24, and 28, the mobility score was determined on days 12 and 28, and the histolopathology of the knee joint was examined on day 29. <br /><br />RESULTS<br />Ethanol extract of Psidium guajava leaves significantly suppressed the swelling of the paws in chronic phase based on increasing of edema (%), while starting on day 20. EEPG at 250 mg/kg was most effective in significantly reducing arthritis scores (p&lt;0.05). Histopathological examination showed repair of the knee joint synovial membrane and cartilage.<br /> <br />CONCLUSIONS<br />Psidium guajava leaf extract is effective in decreasing the inflammatory response and arthritic symptoms in rats with adjuvant-induced arthritis. Psidium guajava leaves can be developed into an alternative anti-arthritis treatment.

scholarly journals Alteration of the Fatty Acid Metabolism in the Rat Kidney Caused by the Injection of Serum from Patients with Collapsing Glomerulopathy

Biomedicines ◽  
2020 ◽  
Vol 8 (10) ◽  
pp. 388
Author(s):  
Elizabeth Soria-Castro ◽  
Verónica Guarner-Lans ◽  
María Elena Soto ◽  
María del Carmen Avila-Casado ◽  
Linaloe Manzano Pech ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Metabolism ◽  
Rat Kidney ◽  
Group Iv ◽  
Sprague Dawley ◽  
Group Iii ◽  
Collapsing Glomerulopathy ◽  
Fa Composition ◽  
Group I ◽  
Sprague Dawley Rats

Patients with collapsing glomerulopathy (CG) have marked proteinuria that rapidly progresses to chronic renal failure. In this study, we investigated if the nephropathy produced in a rat model by the injection of serum from CG patients induced alterations in fatty acid (FA) metabolism. Twenty-four female Sprague-Dawley rats were divided into four groups of six rats each: Group I, control rats (C); Group II, rats that received injections of 1 mL of 0.9% NaCl saline solution (SS); Group III, rats injected with 25 mg/mL of serum from healthy subjects (HS); and Group IV, rats injected with 25 mg/mL of serum from CG patients. In all groups, the systolic blood pressure (SBP), proteinuria, creatinine clearance (CC), cholesterol and total FA composition in the kidney and serum were evaluated. The administration of serum from CG patients to rats induced glomerular collapse, proteinuria, reduced CC and elevated SBP (p ≤ 0.01) in comparison with the C, SS and HS rats. The FA composition of the serum of rats that received the CG serum showed an increase in palmitic acid (PA) and a decrease in arachidonic acid (AA) when compared to serum from HS (p ≤ 0.02). In rats receiving the CG serum, there was also a decrease in the AA in the kidney but there was an increase in the PA in the serum and kidney (p ≤ 0.01). These results suggest that the administration of serum from CG patients to rats induces alterations in FA metabolism including changes in PA and in AA, which are precursors for the biosynthesis of the prostaglandins that are involved in the elevation of SBP and in renal injury. These changes may contribute to collapsing glomerulopathy disease.

scholarly journals DUAL EFFECT OF ATORVASTATIN ON ENDOTHELIUM

2015 ◽  
Vol 22 (09) ◽  
pp. 1196-1202
Author(s):  
Faizania Shabbir ◽  
Muhammad Alamgir Khan ◽  
Tausif Ahmed Rajput
Keyword(s):  
Colorimetric Method ◽  
The Body ◽  
Sprague Dawley ◽  
Dual Effect ◽  
Immunosorbant Assay ◽  
Group Iii ◽  
Medical College ◽  
Group I ◽  
Randomized Control ◽  
Enzyme Linked Immunosorbant Assay

Objective: The objective of the study was to observe the effect of lipid loweringtherapy on homocysteine and TXA2 concentration in obese hyperlipidemic Sprague Dawleyrats. Design: Randomized Control Trial (RCT). Place and Duration of study: The study wasconducted in Department of Physiology and Centre for Research in Experimental and AppliedMedicine (CREAM), Army Medical College, Rawalpindi; and National Institute of Health (NIH)Islamabad over a period of 12 months. Methodology: Ninety healthy Sprague Dawley ratsdivided into three equal groups. Group I (n=30) were healthy controls, group II (n=30) weremade obese and group III (n=30) were obese treated (atorvastatin 10 mg/kg/day orally bygavage method for three weeks). Body weight was recorded thrice weekly, lipid profile wasmeasured by colorimetric method on microlab and homocysteine and TXA2 were measuredby Enzyme Linked Immunosorbant Assay. Results: Serum low density lipoproteins and TXA2decreased after three weeks of atorvastatin administration, elevated HCY concentration in obesehyperlipidemic rats however was not significantly affected. Conclusion: Atorvastatin apart fromlowering lipid levels in the body also reduces TXA2 concentration which is a vasoprotective.Elevated HCY concentration which is deleterious to the endothelium however is not affected.

scholarly journals EVALUATION OF CHONDROPROTECTIVE EFFECTS OF HYALURONIC ACID IN A RAT MODEL OF OSTEOARTHRITIS

2022 ◽  
Vol 71 (6) ◽  
pp. 2189-93
Author(s):  
Noaman Ishaq ◽  
Shabana Ali ◽  
Muhammad Waqar Aslam Khan ◽  
Kulsoom Farhat ◽  
Nausheen Ata ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Treatment Group ◽  
Rat Model ◽  
Cruciate Ligament ◽  
Gait Pattern ◽  
Control Group ◽  
P Value ◽  
Army Medical ◽  
Medical College ◽  
Group I

Objective: To evaluate the chondroprotective effects of hyaluronic acid in a rat model of osteoarthritis. Study Design: Laboratory based experimental study. Place and Duration of Study: Department of Pharmacology, Army Medical College, Rawalpindi, in alliance with National Institute of health, Islamabad and Department of Pathology, Army Medical College Rawalpindi, from Apr to Jun 2019. Methodology: Sixteen (16) rats of Sprague Dawley breed were procured in this study. Osteoarthritis was induced in right knee joint of rats by surgical resection of medial meniscus and anterior cruciate ligament. They were allocated into two (02) groups with eight (08) rats in both groups. Group-I was control group that was treated with 0.2 ml intra articular saline once weekly for four weeks. While group-II was treatment group that was intra particularly administered with 0.2ml hyaluronic acid once weekly for four weeks. One week after the last dosage, gait pattern of the animals was scored. Then animals were sacrificed and a part of proximal tibia was obtained for histopathologic analysis. Results: Mean gait score of control group and treatment group was 3.25 ± 0.707 and 1.00 ± 0.756 respectively with a statistically significant p-value of <0.001, while mean histopathological Modified Mankin score of control and treatment group was 11.5 ± 1.195 and 5.50 ± 1.195 respectively with a significant p-value of <0.001. Conclusion: Intra articular viscosupplementation of hyaluronic acid in rat model of osteoarthritis resulted in improved gait pattern and histopathology.

scholarly journals THE COMBINATION INFUSION OF Talinum paniculatum (Jacq.) Gaertn., Centella asiatica (L.) Urb. AND Curcuma xanthorrhiza Roxb. EFFECTS ON TESTOSTERONE LEVELS AND INTRODUCTION, CLIMBING AND COITUS FREQUENCIES IN MALE SPRAGUE-DAWLEY RATS

2019 ◽  
Vol 12 (1) ◽  
pp. 33-40
Author(s):  
Nuning Rahmawati ◽  
Ika Yanti Marfuatush Sholikhah
Keyword(s):  
Treatment Group ◽  
Antibacterial Effect ◽  
Centella Asiatica ◽  
Control Group ◽  
Sprague Dawley ◽  
Pharmacological Activities ◽  
Testosterone Levels ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Curcuma Xanthorrhiza

ABSTRACT Talinum paniculatum (som Java) reported to have many pharmacological activities such as increasing spermatozoa quality, cell regenerations, fertility, and antibacterial effect both empirically and scientifically. This study aimed to prove the combination infusion effects of som Java, gotu kola, and temulawak on introduction, climbing, and coitus levels as well as testosterone levels in male Sprague Dawley (SD) rats. SD rats were divided into 2 groups, control group received aquadest, treatment group received formula a mixture of som java Talinum paniculatum) root, gotu kola (Centella asiatica) herb, and Javanese turmeric (Curcuma xanthorrhiza) rhizome with the dose of 491,2 mg/200 g BW for 7 consecutive days. Artificial estrus of female SD rats were induced by administering estradiol valerat 2 mg/200 g BW 48 hours before observation. The determined parameters were levels of testosterone as well as introduction, climbing and coitus frequencies. Administration of estradiol valerat led to artificial estrus induction of female SD rats. The administration of the combination infusion dose 491.2 mg/200 g BW significantly increased testosterone level as well as introduction and climbing frequencies of male SD rats as of 27.77%; 86.39% and 69.17% respectively. ABSTRAK Som jawa (Talinum paniculatum) secara empiris dan ilmiah terbukti telah banyak dimanfaatkan oleh masyarakat untuk mengatasi berbagai masalah kesehatan diantaranya untuk meningkatkan kualitas dan kuantitas spermatozoa, regenerasi sel, fertilitas, dan sebagai antibakteri. Penelitian ini dilakukan untuk membuktikan efek peningkatan kadar hormon testosteron serta frekuensi introduction, climbing, dan coitus tikus jantan SD dari pengaruh infusa kombinasi som jawa, pegagan, dan temulawak. Hewan uji tikus galur SD dibagi menjadi 2 kelompok, yaitu kelompok kontrol diberikan akuades, sedangkan kelompok perlakuan diberikan infusa ramuan akar som jawa, herba pegagan, dan rimpang temulawak dosis 491,2 mg/200 g bb selama 7 hari berturut-turut. Induksi estrus artifisial tikus betina dengan pemberian estradiol valerat 2 mg/200 g bb dilakukan 48 jam sebelum pengamatan. Parameter yang diukur meliputi kadar testosterone, frekuensi introduction, climbing, dan coitus. Dibandingkan dengan kelompok kontrol, pemberian infusa ramuan tidak berefek pada frekunsi coitus, namun mampu meningkatkan kadar testosteron serta frekuensi introduction dan climbing tikus jantan sebesar 27,77; 86,39 dan 69,17%.          

Hepatoprotective activity of Tamarindus indica Linn stem bark ethanolic extract against hepatic damage induced by co-administration of antitubercular drugs isoniazid and rifampicin in Sprague Dawley rats

2018 ◽  
Vol 30 (1) ◽  
pp. 131-137 ◽  
Author(s):  
Shaikh Zohra Meena ◽  
Md. Azizur Rahman ◽  
Paramdeep Bagga ◽  
Md. Mujahid
Keyword(s):  
Ethanolic Extract ◽  
Stem Bark ◽  
Hepatoprotective Activity ◽  
Group Iv ◽  
Hepatic Damage ◽  
Sprague Dawley ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Sprague Dawley Rats

Abstract Background Development of drug-induced hepatic damage (DIHD) during chemotherapy is the most common reason for interruption in chemotherapy. This study evaluated the hepatoprotective activity of the ethanolic extract of Tamarindus indica stem bark (EETI) against the induced DIHD in Sprague Dawley rats. Methods The rats were divided into five groups (n=5). Group I, group III, group IV, and group V rats received 1 mL 1% carboxymethyl cellulose, EETI 100 mg/kg body weight (b.wt), EETI 200 mg/kg b.wt, and silymarin 100 mg/kg b.wt, respectively, orally once every day for 28 days. After 1 h–group II, group III, group IV, and group V rats were administered with isoniazid (INH) and rifampicin (RIF) 50 mg/kg b.wt each orally once every day for 28 days. Then, 24 h after the last dosing, blood was withdrawn from the rats and analyzed for liver specific enzymes and biochemical markers. They were examined for histopathology. Results Co-administration of INH and RIF in group II significantly increased alanine transaminase, aspartate transaminase, alkaline phosphatase, lactate dehydrogenase, serum bilirubin, and cholesterol levels while reduced the total protein and albumin levels compared to that of group I. EETI in group III and group IV rats significantly restored the liver specific enzymes and biochemical markers altered due to co-administration of INH and RIF to normal in a dose-dependent manner. EETI 200 mg/kg b.wt showed better protection to liver than EETI 100 mg/kg b.wt and was comparable to silymarin 100 mg/kg b.wt. It was well supported with histopathology of liver tissues. Conclusions EETI possesses hepatoprotective activity against DIHD in rats. It may have a substantial impact on developing clinical strategies to treat patients with hepatic damage.

scholarly journals A COMPARISON OF BODY MASS INDEX AND WEIGHT GAIN AMONG MALE SPRAGUE DAWLEY RATS ON BROILER AND DOMESTIC CHICKEN MEAT FEED

2020 ◽  
Vol 70 (6) ◽  
pp. 1662-65
Author(s):  
Hammad Gul Khan ◽  
Amir Rashid ◽  
Zainab Khan ◽  
Faiza Aman ◽  
Muhammad Shoaib
Keyword(s):  
Weight Gain ◽  
Broiler Chicken ◽  
Diet Group ◽  
Chicken Meat ◽  
Control Group ◽  
P Value ◽  
Sprague Dawley ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Pelleted Diet

Objective: To compare and evaluate the effects of broiler chicken fed with commercially offered feed and chicken fed with organic diet on BMI and weight gain in Sprague Dawley rats. Study Design: A randomized controlled trial. Place and Duration of Study: Multi-Disciplinary Laboratory of Department of Biochemistry and Molecular Biology, Army Medical College, Rawalpindi, and collaborated with Quaid-e-Azam University, Islamabad from November 2017 to April 2019. Methodology: Ninety male early weaned Sprague Dawley rats were arbitrarily assorted into three groups (n=30). Group I control rats were nourished on standard pelleted diet. Group II rats were nourished with organic chicken meat along with their standard pelleted diet. Group III rats were nourished with broiler chicken meat along with their standard pelleted diet. BMI and weight gain were estimated. All variables were calculated as Mean ± SD values. One-way ANOVA was applied to determine the significance among groups followed by Tuckey’s HSD post hoc test. p value ≤ 0.05 was considered significant. Results: The current study demonstrated significant increase in BMI (p ≤ 0.001) and weight gain (p ≤ 0.001) in both experimental groups as compared to control group. Conclusion: Based on the findings of our study we propose that broiler chicken meat consumption could be the probable cause of weight imbalances and out of proportion gain of weight and growth in experimental rats.

Parathyroid hormone contributes to volume expansion-induced inhibition of proximal reabsorption

1985 ◽  
Vol 248 (1) ◽  
pp. F100-F103 ◽  
Author(s):  
O. Mercier ◽  
M. Bichara ◽  
M. Paillard ◽  
J. P. Gardin ◽  
F. Leviel
Keyword(s):  
Parathyroid Hormone ◽  
Volume Expansion ◽  
Sprague Dawley ◽  
Nacl Transport ◽  
Free Expansion ◽  
Group Iii ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Stimulation Of ◽  
Intact Rats

Volume expansion inhibits the proximal reabsorption of water, bicarbonate, and chloride. The present work tested a hypothetical role of parathyroid hormone (PTH) in the expansion effect. We studied 19 Sprague-Dawley rats during a plasma-replete euvolemic state and following 10% body wt colloid-free expansion. In group I, six intact rats, volume expansion decreased plasma ionized calcium concentration ([Ca2+]P) from 2.28 +/- 0.06 to 2.11 +/- 0.04 meq/liter and increased nephrogenous cAMP (NcAMP) from 29 +/- 5 to 66 +/- 10 pmol X min-1 X g kidney wt-1. In group II, six acutely thyroparathyroidectomized (TPTX) rats, [Ca2+]P also fell from 2.18 +/- 0.08 to 1.80 +/- 0.08 meq/liter but NcAMP did not rise significantly (9 +/- 3 vs. 17 +/- 5 pmol X min-1 X g kidney wt-1). These data strongly suggest that stimulation of PTH activity occurred during expansion in intact animals. In group III, seven TPTX rats, volume expansion inhibited proximal reabsorption of total CO2 by 11%, of chloride by 24%, and of water by 19%. Volume expansion-induced reduction in bicarbonate, chloride, and water reabsorption was smaller in TPTX than in intact rats previously studied. We conclude that volume expansion inhibits proximal reabsorption in part by decreasing the active transcellular NaHCO3 and NaCl transport secondary to stimulation of PTH activity.

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