scholarly journals THE COMBINATION INFUSION OF Talinum paniculatum (Jacq.) Gaertn., Centella asiatica (L.) Urb. AND Curcuma xanthorrhiza Roxb. EFFECTS ON TESTOSTERONE LEVELS AND INTRODUCTION, CLIMBING AND COITUS FREQUENCIES IN MALE SPRAGUE-DAWLEY RATS

2019 ◽  
Vol 12 (1) ◽  
pp. 33-40
Author(s):  
Nuning Rahmawati ◽  
Ika Yanti Marfuatush Sholikhah
Keyword(s):  
Treatment Group ◽  
Antibacterial Effect ◽  
Centella Asiatica ◽  
Control Group ◽  
Sprague Dawley ◽  
Pharmacological Activities ◽  
Testosterone Levels ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Curcuma Xanthorrhiza

ABSTRACT Talinum paniculatum (som Java) reported to have many pharmacological activities such as increasing spermatozoa quality, cell regenerations, fertility, and antibacterial effect both empirically and scientifically. This study aimed to prove the combination infusion effects of som Java, gotu kola, and temulawak on introduction, climbing, and coitus levels as well as testosterone levels in male Sprague Dawley (SD) rats. SD rats were divided into 2 groups, control group received aquadest, treatment group received formula a mixture of som java Talinum paniculatum) root, gotu kola (Centella asiatica) herb, and Javanese turmeric (Curcuma xanthorrhiza) rhizome with the dose of 491,2 mg/200 g BW for 7 consecutive days. Artificial estrus of female SD rats were induced by administering estradiol valerat 2 mg/200 g BW 48 hours before observation. The determined parameters were levels of testosterone as well as introduction, climbing and coitus frequencies. Administration of estradiol valerat led to artificial estrus induction of female SD rats. The administration of the combination infusion dose 491.2 mg/200 g BW significantly increased testosterone level as well as introduction and climbing frequencies of male SD rats as of 27.77%; 86.39% and 69.17% respectively. ABSTRAK Som jawa (Talinum paniculatum) secara empiris dan ilmiah terbukti telah banyak dimanfaatkan oleh masyarakat untuk mengatasi berbagai masalah kesehatan diantaranya untuk meningkatkan kualitas dan kuantitas spermatozoa, regenerasi sel, fertilitas, dan sebagai antibakteri. Penelitian ini dilakukan untuk membuktikan efek peningkatan kadar hormon testosteron serta frekuensi introduction, climbing, dan coitus tikus jantan SD dari pengaruh infusa kombinasi som jawa, pegagan, dan temulawak. Hewan uji tikus galur SD dibagi menjadi 2 kelompok, yaitu kelompok kontrol diberikan akuades, sedangkan kelompok perlakuan diberikan infusa ramuan akar som jawa, herba pegagan, dan rimpang temulawak dosis 491,2 mg/200 g bb selama 7 hari berturut-turut. Induksi estrus artifisial tikus betina dengan pemberian estradiol valerat 2 mg/200 g bb dilakukan 48 jam sebelum pengamatan. Parameter yang diukur meliputi kadar testosterone, frekuensi introduction, climbing, dan coitus. Dibandingkan dengan kelompok kontrol, pemberian infusa ramuan tidak berefek pada frekunsi coitus, namun mampu meningkatkan kadar testosteron serta frekuensi introduction dan climbing tikus jantan sebesar 27,77; 86,39 dan 69,17%.          

scholarly journals Synergistic suppression of pre-perfusion of donor livers with recipient serum and cobra venom factor treatment on hyperacute rejection following liver xenotransplantation

2012 ◽  
Vol 27 (5) ◽  
pp. 301-305 ◽  
Author(s):  
Baohua Zhu ◽  
Chuanming Tong ◽  
Weitao Guo ◽  
Rong Pu ◽  
Guoping Zhang ◽  
...  
Keyword(s):  
Survival Time ◽  
Hyperacute Rejection ◽  
Cobra Venom ◽  
Control Group ◽  
Sprague Dawley ◽  
Donor Liver ◽  
Cobra Venom Factor ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
And Control

PURPOSE: To investigate synergistic suppression of donor liver pre-perfusion with recipient serum (RS) and cobra venom factor (CVF) treatment on hyperacute rejection (HAR) following liver xenotransplantation. METHODS: Guinea-pigs (GP, n=24) and Sprague-Dawley rats (SD, n=24) were recruited. Before transplantation, serum was collected from SD rats and used for preparation of inactivated complements. GP and SD rats were randomly assigned into four groups (n=6), respectively: RS group, CVF group, RS+CVF group and control group. Orthotopic liver xenotransplantation was performed with modified two-cuff technique. The survival time and liver function of recipients, morphological and pathological changes in rat livers were investigated. RESULTS: There was no piebald like change in the recipient livers in all experiment groups. The survival time of recipients in all experiment groups was longer than that in control group (p<0.05). Moreover, the survival time in the RS+CVF group was markedly longer than that in the RS group (p<0.01) and CVF group (p<0.05). The serum ALT level in all experiment groups were lower than that in the control group (p<0.05). Furthermore, the ALT level in the RS+CVF group was significantly lower than that in the CVF group (p<0.05) and RS group (p<0.01). The histological damages were significantly improved when compared with the control group, and the histological damages in the RS+CVF group were milder than those in the remaining groups (p<0.05) CONCLUSION: Pre-perfusion of donor liver with recipient serum and cobra venom factor treatment can exert synergistic suppressive effects on the hyperacute rejection following liver xenotransplantation.

The effect of single clove Black garlic on the hemostasis status and lipid profile in male Sprague Dawley rats with non-alcoholic fatty liver disease

10.5219/1516 ◽  
2021 ◽  
Vol 15 ◽  
pp. 387-395
Author(s):  
Lili Nurmawati ◽  
Mohammad Sulchan ◽  
Siti Fatimah-Muis ◽  
Hery Djagat Purnomo ◽  
Kis Djamiatun ◽  
...  
Keyword(s):  
Liver Disease ◽  
Treatment Group ◽  
Fatty Liver Disease ◽  
Lipid Profiles ◽  
Control Group ◽  
Sprague Dawley ◽  
Alcoholic Fatty Liver ◽  
Sprague Dawley Rats ◽  
Alcoholic Fatty Liver Disease ◽  
Pai 1

Non-alcoholic fatty liver disease (NAFLD) patients have alteration in hemostasis components. Thus, including excess expression of plasminogen activator inhibitor-1 (PAI-1), causing fibrinolysis disorders; the majority of these patients are hypercoagulable state prone to thrombosis. Some evidence suggests that garlic and garlic supplements have antithrombotic and anti-inflammatory properties. Besides, garlic stimulates fibrinolytic activity and normalizes plasma lipid imbalances. Black garlic is processed garlic that is produced through natural aging at a controlled temperature (70 °C) and high humidity (90%) for several days, without other additives. This study aimed to prove the effect of single clove black garlic (SCBG) (Allium sativum Linn) on PAI-1 levels and lipid profiles of NAFLD rats induced by a high-fat fructose diet (HFFD) containing 1.25% cholesterol and 0.5% cholic acid.The rats were then divided into healthy control group (K1+); NAFLD control group without treatment (K2+); 0.9 mg simvastatin treatment group (K1-); 45 mg metformin treatment group (K2-); SCBG 450 mg per 200g BW (X1); 900 mg per 200 g BW (X2); and 1350 mg per 200 g BW (X3). All treatments were administered for 4 weeks via oral gavage. As a result, significant differences in PAI-1 levels and lipid profiles between groups after the administration (p = 0.001) were noted and also by simvastatin and metformin, respectively. There was a correlation between PAI-1 and lipid profile of SCBG treatment. In conclusion, the administration of SCBG (1350 mg per 200 g BB per day) for 4 weeks had a significant effect on PAI-1 levels, and the lipid profiles in Sprague Dawley rats modeled NAFLD (p = 0.001). SCBG has provided benefits that can be useful in the management of NAFLD but it’s not equivalent to medicine.

scholarly journals ANTI-INFLAMMATORY EFFECT OF ZINGIBER OFFICINALE ON SPRAGUE DAWLEY RATS

2017 ◽  
Vol 10 (3) ◽  
pp. 353 ◽  
Author(s):  
Rohini Karunakaran ◽  
Ndanusa Abdullahi Hassan ◽  
Uma Sankar A ◽  
Khin Mar Aye
Keyword(s):  
Aqueous Extract ◽  
Diclofenac Sodium ◽  
Zingiber Officinale ◽  
Control Group ◽  
Sprague Dawley ◽  
Reference Drug ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Anti Inflammatory ◽  
Inflammatory Effect

ABSTRACTObjective: To investigate the anti-inflammatory effect of aqueous extract of Zingiber officinale on carrageenan-induced inflammation on SpragueDawley (SD) rats.Methods: SD rats were divided into six of five groups and allowed to acclimatize for 1 week. Inflammation was induced on the animal by injecting theright hand paw with carrageenan (0.1 ml of 1%). Group 1 was given normal saline and served as a control. Group 2 was fed with food and water andserved as the carrageenan control. Group 3 was given 200 mg/kg aqueous extract of ginger, Group 4 with 400 mg/kg aqueous extract of ginger, andGroup 5 with 150 mg/kg diclofenac sodium (reference drug for inflammation).Results: The paw edema in carrageenan-induced SD rats was considerably reduced by treating with 400 mg/kg aqueous ginger extracts whencompared to the untreated SD rats (p<0.001).Conclusion: This study indicates that aqueous extract of Z. officinale possesses anti-inflammatory properties.Keywords: Anti-inflammatory, Sprague Dawley rats, Zingiber officinale, Carrageenan, Edema

scholarly journals Centella asiatica increases B-cell lymphoma 2 expression in rat prefrontal cortex

2016 ◽  
Vol 34 (1) ◽  
pp. 10
Author(s):  
Kuswati Kuswati ◽  
Djoko Prakosa ◽  
Brian Wasita ◽  
Nanang Wiyono
Keyword(s):  
Prefrontal Cortex ◽  
B Cell ◽  
Restraint Stress ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
B Cell Lymphoma ◽  
Control Group ◽  
Sprague Dawley ◽  
Stress Group ◽  
Sprague Dawley Rats

<p>BACKGROUND<br />Stress is one of the factors that cause apoptosis in neuronal cells. Centella<br />asiatica has a neuroprotective effect that can inhibit apoptosis. This study<br />aimed to examine the effect of Centella asiatica ethanol extract on B-cell<br />lymphoma 2 (Bcl-2) protein expression in the prefrontal cortex of rats.</p><p>METHODS<br />An experimental study was conducted on 34 brain tissue samples from male<br />Sprague Dawley rats exposed to chronic restraint stress for 21 days. The<br />samples were taken from following groups: non-stress group K, negative<br />control group P1 (stress + arabic gum powder), P2 (stress + C.asiatica at<br />150 mg/kgBW), P3 (stress + C.asiatica at 300 mg/kg BW), P4 (stress +<br />C.asiatica at 600 mg/kg body weight) and positive control group P5 (stress<br />+ fluoxetine at 10 mg/kgBW). The samples were made into sections that<br />were stained immunohistochemically using Bcl-2 antibody to determine the<br />percentage of cells expressing Bcl-2. Data were analyzed using one way<br />ANOVA test followed by a post - hoc test.</p><p>RESULTS<br />There were significant differences in mean Bcl-2 expression between the<br />groups receiving Centella asiatica compared with the non-stress group and<br />stress-only group (negative control group) (p&lt;0.05). The results were<br />comparable to those of the fluoxetine treatment group.</p><p>CONCLUSION</p><p>The Centella asiatica ethanol extract was able to increase Bcl-2 expression<br />in the prefrontal cortex of Sprague Dawley rats exposed to restraint stress.<br />This study suggests that Centella asiatica may be useful in the treatment of<br />cerebral stress.</p>

scholarly journals Red Guava Juice (Psidium guajava L.) Reduce Oxidative Stress of Toll Gate Collector

2019 ◽  
Vol 39 (4) ◽  
pp. 333
Author(s):  
Riva Mustika Anugrah ◽  
Sugeng Maryanto ◽  
Kusmiyati Tjahjono ◽  
Martha Irene Kartasurya
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Initial Conditions ◽  
Thiobarbituric Acid ◽  
Psidium Guajava ◽  
T Test ◽  
Wilcoxon Test ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Exposure to air pollution can increase the occurrence of oxidative stress. Research showed that guava can reduce oxidative stress in Sprague Dawley rats. This experiment investigated the effect of red guava juice on oxidative stress in toll collectors. The subjects were 20 toll collectors in the treatment group and 20 in the control group. The treatment group received 250 mL red guava juice for 21 days while the controls did not receive anything. Malondialdehyde (MDA) was measured with thiobarbituric acid (TBA) reaction, food consumption was measured by 3×24 hours’ recall. The data were analyzed by paired t-test, Wilcoxon test, independent t-test, Mann Whitney test, and general linear model. The results showed that the effect of red guava juice on the MDA level was significant in the treatment group (p<0.05). A multivariate analysis showed that the effect of red guava juice to MDA level was significant after controlled by age, carbohydrate intake and initial conditions (p<0.05). Red guava juices of 250 mL for 21 days could reduce malondialdehyde (MDA) levels of toll collectors.

scholarly journals THE ROLE OF VITAMIN E (α-TOCOPHEROL) ON TESTOSTERONE LEVEL IN SPRAGUE DAWLEY RATS FOLLOWING CISPLATIN TREATMENT

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
Dian Kartika Rezia ◽  
Lukman Hakim ◽  
Wahjoe Djatisoesanto
Keyword(s):  
Vitamin E ◽  
Adult Male ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Testosterone Levels ◽  
White Rats ◽  
Sprague Dawley Rats

Objective: To observe the difference of testosterone levels in adult male Sprague Dawley rats treated with combination of cisplatin and vitamin E compared to those treated with cisplatin only. Material & Methods: We used 24 adult male Sprague Dawley rats weight 200–300 grams and randomly assigned into 4 groups (n=6). Rats in negative control group (NC) were given intraperitoneal normal saline injection, while the positive control (PC) group were injected with cisplatin 5 mg/kgBW at the end of the 3rd week. Two other groups, P1 and P2, were injected with cisplatin 5 mg/kgBW and given vitamin E orally 50 mg/kgBW and 200 mg/kgBW, respectively. Cardiac blood was aspirated at the end of the 7th week and processed for analysis of testosterone levels. Results: We recorded a significantly lower testosterone levels in rats treated only with cisplatin 5 mg/kgBW (CP) compared to those in CN group (p=0.006), and those receiving combination of cisplatin and vitamin E 50 mg/kgBW (p=0.003) and 200 mg/kgBW (p=0.001). Though not significant, testosterone levels were higher in P2 group than in P1 group (p=0.702). Conclusion: Exposure to cisplatin can lower testosterone levels in white rats, and the administration of vitamin E gives protection against such effect. 

COMPARISON OF TWO BIOMODELS (BALB/C MICE AND SPRAGUE DAWLEY RATS) IN THE ALKALINE COMET ASSAY

2020 ◽  
Vol 4 (13-16) ◽  
Author(s):  
Daniel Francisco Arencibia Arrebola ◽  
Luis Alfredo Rosario Fernández ◽  
Yolanda Emilia Suárez Fernández ◽  
Alexis Vidal Novoa
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood ◽  
Species Group ◽  
Negative Control ◽  
Control Group ◽  
Alkaline Comet Assay ◽  
Sprague Dawley ◽  
Sd Rats ◽  
Sprague Dawley Rats

<p>This article had as objective to carry out a comparison between Balb/c mice and Sprague Dawley rats as biomodel in the alkaline comet assay, keeping in mind the basal frequency and that induced with Cyclophosphamide, the induction of single strand breaks (SSB) or alkali-labile sites formation on DNA of peripheral blood leukocytes. Ten animal/sex/species/group of Balb/c mice and Sprague Dawley rats were used, and treated for 14 days. Using a negative control group (not treated), two substance-vehicle controls and a positive control received cyclophosphamide 50 mg/kg, via intraperitoneal. After two weeks the alkaline electrophoresis gel was performed for individual cells from leukocytes of peripheral blood to screen for possible DNA damage. The best biomodel in both sexes was the SD rats differing significantly with the results obtained in Balb/c mice keeping in mind the spontaneous and induced values of DNA damage. This study shows SD rats are a more efficient model for preclinical genotoxic evaluation of drugs, vaccines and natural products.</p>

scholarly journals EFFECT OF OBESTATIN ON PITUITARY GONADAL AXIS, LEPTIN AND MDA LEVELS IN OBESE SPRAGUE DAWLEY RATS

2021 ◽  
Vol 71 (3) ◽  
pp. 1061-65
Author(s):  
Nadia Latif ◽  
Amina Rasul ◽  
Sajid Ali ◽  
Rimsha Zia
Keyword(s):  
Obese Group ◽  
Control Group ◽  
Sprague Dawley ◽  
Group Iii ◽  
Army Medical ◽  
Testosterone Levels ◽  
Medical College ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Obese Rats

Objective: To determine the effect of obestatin administration on FSH, LH, testosterone, leptin and MDA levels in obese Sprague Dawley Rats. Study Design: Laboratory based animal study. Place and Duration of Study: Physiology department, Army Medical College Rawalpindi, from Mar to Jun 2015. Methodology: This randomized controlled trial was conducted at Physiology Department Army medical college. Male healthy Sprague Dawley rats were randomly divided into 3 groups (n–15 each) i.e. control group (group I) fed with normal pellet diet (NPD), obese group (group II) and obestatin treated obese group (group III) fed with high fat diet (HFD). After 10 weeks, group III was treated with obestatin (1nmol/100ml intraperitoneally). Blood samples were obtained by terminal intracardiac sampling for bioasssays of FSH, LH, testosterone, leptin and MDA by ELISA. Results: Obestatin supplementation in obese rats showed significant increase in LH levels (3.79 ± 0.05) and testosterone levels (2.07 ± 0.22) when compared to the non treated obese rats (2.19 ± 0.07) and (1.37 ± 0.15) respectively while significant decrease in leptin (3.85 ± 0.23) and MDA levels (1.62 ± 0.07) was observed when compared to the non-treated control groups (6.10 ± 1.18) and (1.95 ± 0.07) respectively. However, serum FSH levels remained unchanged among the treated and nontreated groups. Conclusion: Obestatin increases the testosterone levels by augmenting the pituitary gonadal axis through decrease in the oxidative stress and leptin levels in obese rats.

scholarly journals The Effect of Myrmecodia pendans Ethanol Extract on Inflamed Pulp: Study on Sprague Dawley Rats

2019 ◽  
Vol 3 (2) ◽  
pp. 115
Author(s):  
Janti Sudiono ◽  
Meylisa Hardina
Keyword(s):  
Treatment Group ◽  
Healing Process ◽  
Ethanol Extract ◽  
Flavonoid Compound ◽  
Control Group ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Group Ii

Background: Inflammation is a body response caused by injury and infection. Pulpitis is a pulp tissue inflammation which is the continuous process of pulp hyperemia by bacteria invasion. Myrmecodia pendans or Sarang semut is known to contain flavonoid compound which has the anti inflammation effect. The purpose of this study is to investigate the effect of Myrmecodia pendans ethanol extract on the healing process of pulp inflammation.Materials and Methods: This experimental study involved pre- and post-in vivo treatment of 27 Sprague Dawley rats in which the inducted pulpitis model was obtained by injecting 0.01 mL Porphyromonas gingivalis into the dental pulp for 48 hours. Subjects were divided randomly into Group I (negative control), Group II (pulpitis treated by Myrmecodia pendans extract ethanol as treatment group), and Group III (pulpitis treated by Ca(OH)2 as positive control group). Group II and III as pulpitis treatment groups were divided into subgroups based on the induction periods of 48 hours (2 days), 168 hours (7 days), and 366 hours (14 days). All specimens were processed into the slides and evaluated microscopically for the healing process.Results: The result of this study showed significant difference (p<0.05) among groups on day 2, 4 and 7. On day 4, the pulpitis treatment group of Myrmecodia pendans extract showed better healing process than Ca(OH)2. On day 7, the pulpitis treatment group of Ca(OH)2 showed better healing process than Myrmecodia pendans extract. On day 14, both of the pulpitis treatment groups showed normal pulp.Conclusion: Myrmecodia pendans ethanol extract is effective for the healing process of inflamed pulp.Keywords: inflamed pulp, Myrmecodia pendans, sarang semut, Ca(OH)2 , healing process

scholarly journals Polymerase chain reaction assay for the diagnosis of experimentally infected pregnant Sprague-Dawley rats with Brucella abortus biotype 1

2012 ◽  
Vol 49 (No. 7) ◽  
pp. 253-258
Author(s):  
Rahman MS
Keyword(s):  
Polymerase Chain Reaction ◽  
Brucella Abortus ◽  
Brucella Melitensis ◽  
Control Group ◽  
Sprague Dawley ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Polymerase Chain

In order to diagnose the experimentally infected pregnant Sprague-Dawley (SD) rats with Brucella abortus biotype 1 using polymerase chain reaction (PCR) assay, the SD rats were injected subcutaneously at the dose of 1.0 &times; 10<sup>9</sup> colony forming units (cfu) at different stages of gestation period. The maximum rectal temperature was recorded as 38&deg;C in the infected group within 3 days, whereas in the control group the temperature remained normal (36&deg;C). There were no stillbirths, abortions or premature birth and relapsing fever in the infected SD rats. The pathological findings of infected SD rats were splenomegaly, metritis, swelling of lymph nodes, placentitis associated with lymphocytic and macrophage infiltration. Four hundred ninety-eight base pair DNA was detected in infected tissues through AMOS (Brucella abortus, Brucella melitensis, Brucella ovis, Brucella suis) PCR assay. The AMOS PCR assay was shown to be a valuable tool for&nbsp;diagnosis of infected pregnant Sprague-Dawley rats with B.&nbsp;abortus biotype 1.

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