IDENTIFIKASI DAN PENETAPAN KADAR RHODAMIN B DALAM KERUPUK BERWARNA MERAH YANG BEREDAR DI PASAR ANTASARI KOTA BANJARMASIN

2017 ◽  
Vol 1 (1) ◽  
pp. 85
Author(s):  
Eka Kumalasari
Keyword(s):  
Thin Layer ◽  
Rhodamine B ◽  
Textile Industry ◽  
Mobile Phase ◽  
Uv Lamp ◽  
Tapioca Flour ◽  
Red Dye ◽  
Layer Chromatography ◽  
Rhodamin B

Crackers are made from tapioca flour batter mixed with flavorings and colorings, still many outstanding crackers that contain ingredients banned dye Rhodamine B. Rhodamine B is a chemical used for red dye in the textile industry and plastic. Rhodamine B can cause cancer, poisoning, lung irritation, sore eyes, and sore throat. This study aims to identify and determination the levels of Rhodamine B in circulating red crackers Antasari market Banjarmasin.The population is that sold in the red crackers that sold in Antasari market Banjarmasin.. The sampling is technique incidental sampling , that is based on chance, so any population by chance met with researchers can be used as a sample. Identification of Rhodamine B was done by Thin Layer Chromatography (TLC) by using the stationary phase silica GF 254 and mobile phase is elution solvent is n-butanol, ethyl acetate, ammonia (10:4:5). Then detected with a UV lamp 254 nm and 366 nm. While for the determination of levels using Vis spectrophotometry at a wavelength of 544 nm.The results showed that the samples of 6 found one sample containing Rhodamine B, namely samples 5 (cassava crackers matches) and obtained values of 7,25 ± 3,8640 levels mg / kg. Based on these results, Rhodamine B still found in crackers that sold in the market Antasari Banjarmasin.

scholarly journals Identifikasi dan Penetapan Kadar Rhodamin B pada Eye Shadow secara Kromatografi Lapis Tipis dan Spektrofotometri Ultraviolet-Visible

2020 ◽  
Vol 19 (02) ◽  
pp. 81-86
Author(s):  
Siva Fauziah ◽  
Dede Komarudin ◽  
Citra Dewi
Keyword(s):  
Respiratory Tract ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rhodamine B ◽  
Uv Light ◽  
Average Value ◽  
Traditional Market ◽  
Layer Chromatography ◽  
Rhodamin B

Abstract Based on BPOM findings in 2016 there is still the presence of Rhodamin B which is used as one of the dyes in cosmetics. It causes irritation to the skin, respiratory tract and is carcinogenic. The purpose of this study to conduct an examination and determination of Rhodamin B in eye shadow in the Kalideres traditional market. The sample used for the research is eye shadow that is bought from cosmetics stores and cosmetic kiosks, which circulated in the Kalideres traditional market. The sample consist of five different brands based on three criteria is eye shadow which does not include the ingredients used, the writing in the packaging used a language other than Indonesia and does not have a permit number from BPOM. This research was conducted to identify the presence of Rhodamin B in eye shadow with KLT method ( thin layer chromatography), the eluent used is N-butanol, etil asetat, amoniac 25% (10 : 4 : 5) then detected with UV light 254 nm and determination of the content using UV-Vis spectrophotometry method at maximum wavelength 553 nm using as blank is methanol. The resulted was presence of Rhodamine B in sample code A and sample code D with the average value of Rhodamine B levels in the sample examined, the sample code A is 1,3063 mg/g and sample code D is 1,2564 mg/g.   Keywords: Rhodamine B, Eye Shadow, KLT, Uv-Vis Spectrophotometry  

scholarly journals Determination of New Antidepressants in Pharmaceuticals by Thin-Layer Chromatography with Densitometry

2010 ◽  
Vol 93 (3) ◽  
pp. 778-782 ◽  
Author(s):  
Tatána Gondová ◽  
Iveta Petríková
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Simultaneous Separation ◽  
Tlc Plates ◽  
Precision And Accuracy ◽  
Layer Chromatography

Abstract A new and simple TLC-densitometry method has been developed for the simultaneous separation of the two noradrenergic and specific serotonergic antidepressants mirtazapine and mianserine and validated for their determination in commercially available tablets. The method used TLC plates precoated with silica gel 60F254 as the stationary phase, and the mobile phase consisted of hexaneisopropanol25 ammonia (70 + 25 + 5, v/v/v). Densitometric analysis was carried out in the absorbance mode at 280 nm. The method was validated in accordance with International Conference on Harmonization guidelines in terms of linearity, LOD, LOQ, precision, and accuracy. Calibration curves were linear (R2 > 0.9970) with respect to peak area in the concentration range of 5002500 and 5005000 ng/spot for mirtazapine and mianserine, respectively. The LODs were 20 and 35 ng/spot for mirtazapine and mianserine, respectively. The described method was successfully applied to the determination of mirtazapine and mianserine in their pharmaceutical formulations with recovery ranging from 99.83 to 101.20 of the labeled amount of the compounds. The proposed method can be used in routine QC of these drugs in pharmaceutical formulations.

scholarly journals Qualitative Analysis of Rhodamine B in Shrimp Paste at Sumbawa Besar City Markets Using Thin Layer Chromatography

2021 ◽  
Vol 8 (2) ◽  
pp. 34
Author(s):  
Abby Rahmat Kamaruzzaman ◽  
Asri Darmawati ◽  
Djoko Agus Purwanto
Keyword(s):  
Qualitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rhodamine B ◽  
Layer Chromatography ◽  
Rhodamin B

Terasi biasa diberi pewarna merah agar lebih menarik. Tahun 2013, pernah ditemukan Rhodamin B dalam produk terasi yang beredar di pasar kabupaten Sumbawa Barat. Rhodamin B adalah pewarna merah yang dilarang digunakan untuk makanan. Diantara berbagai produk terasi yang dijual di Sumbawa, terasi khas asli Sumbawa yaitu terasi Empang, perlu dijaga keamanannya agar dapat lebih dipromosikan. Tujuan penelitian ini adalah membuktikan bahwa terasi yang dijual pasar kota Sumbawa Besar tidak mengandung Rhodamin B. Metode yang digunakan untuk identifikasi Rhodamin B adalah kromatografi lapis tipis (KLT). Penelitian ini menggunakan silika gel 60 F254 sebagai fase diam dan fase gerak terpilih yaitu N-butanol:etil asetat:amonia 25% (10:4:5). Sampel adalah produk terasi yang dijual di Pasar Kota Sumbawa Besar, periode sampling bulan Februari dan Maret 2021. Preparasi sampel terasi dengan cara diekstraksi menggunakan etanol dan volume ekstrak yang ditotolkan pada lempeng KLT adalah 2 μl. Parameter validasi metode yang diuji adalah spesifisitas/selektifitas dan batas deteksi (LOD). Hasil penelitian menunjukkan metode ini spesifik untuk Rhodamine B yaitu Rf 0,69, profil spektra yang sama antara baku dan sampel yang diadisi Rhodamin B. Resolusi (Rs) antara noda Rhodamin B dengan noda terdekat lain dalam sampel adalah >1,5. Panjang gelombang serapan maksimum Rhodamin B adalah 544 nm. Nilai LOD sebesar 4,14 ng. Hasil identifikasi terhadap 10 sampel yang terdiri dari 3 sampel terdaftar di BPOM dan 7 sampel tidak terdaftar di BPOM tidak terdeteksi adanya Rhodamin B. Kesimpulan dari penelitian ini adalah semua sampel terasi yang dijual di pasar kota Sumbawa Besar tidak terdeteksi mengandung Rhodamin B.

IDENTIFIKASI DAN PENETAPAN KADAR RHODAMIN B PADA KUE BERWARNA MERAH DI PASAR ANTASARI KOTA BANJARMASIN

2017 ◽  
Vol 1 (1) ◽  
pp. 75
Author(s):  
Ratih Pratiwi Sari
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rhodamine B ◽  
Textile Industry ◽  
Skin Irritation ◽  
Sampling Technique ◽  
Visible Spectrophotometry ◽  
Nasal Irritation ◽  
High Concentrations ◽  
Layer Chromatography

Rhodamine B is a synthetic dye used to dye the textile industry. Rhodamine B is presence in food can cause poisoning, skin irritation, lung irritation, eye irritation, throat irritation, nasal irritation, and cause liver damage if exposed to high concentrations. Samples taken from the red cake seller in the Antasari Banjarmasin market. This research is a descriptive study. The sampling technique used was accidental sampling. Rhodamine B on a method of identification of samples using Thin Layer Chromatography and Visible spectrophotometry. Samples were prepared using the absorption method wool. The resulting solution will be used as identification in Thin Layer Chromatography using silica gel GF 254 plates with a mobile phase of n-butanol : ethyl acetate : ammonia (10:4:5). Rhodamine B assay performed visible spectrophotometry at a wavelength of 544 nm.Results of identification were putri ayu cake, Apam cake, Kukus cake A, Bolu cake, Singkong cake, and Kukus cakes B, shows that 1 positive samples containing Rhodamine B is a Apam cake. After that, the assay of Rhodamine B was performed in the sample apam  cake and obtained for 0,4229 ± 0,1157 mg of Rhodamine B in 1 piece of red cake

scholarly journals Column and Thin-Layer Chromatographic Methods for the Simultaneous Determination of Acediasulfone in the Presence of Cinchocaine, and Cefuroxime in the Presence of Its Hydrolytic Degradation Products

2007 ◽  
Vol 90 (2) ◽  
pp. 405-413 ◽  
Author(s):  
Mohammad Abdul-Azim Mohammad ◽  
Nagwan H Zawilla ◽  
Fawzy M El-Anwar ◽  
Samir M El-Moghazy Aly
Keyword(s):  
Thin Layer ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
Degradation Products ◽  
Hydrolytic Degradation ◽  
Ammonium Hydroxide ◽  
Dosage Forms ◽  
Ultraviolet Detection ◽  
Layer Chromatography

Abstract Column liquid chromatography (LC) and thin-layer chromatography (TLC)densitometry methods are described for simultaneous determination of acediasulfone (Ace) and cinchocaine (Cinco). In the LC method, the separation and quantitation of the 2 drugs was achieved on a Zorbax C8 column (5 μm, 150 × 4.6 mm id) using a mobile phase composed of methanol-phosphate buffer, pH 2.5 (66 + 34, v/v), at a flow rate of 1 mL/min and ultraviolet detection at 300 and 327 nm for Ace and Cinco, respectively. The method showed linearity over concentration ranges of 20-200 and 45685 μg/mL, respectively. In the TLCdensitometry method, a mobile phase composed of methanol-tetrahydrofuran-acetic acid (45 + 5 + 0.5, v/v/v) was used for the separation of the 2 drugs. The linearity range was 0.5-4 and 2-9 μg/spot, respectively. In addition, stability indicating TLCdensitometry method has been developed for determination of cefuroxime sodium in the presence of 570% of its known hydrolytic degradation products. The mobile phase butanol-methanol-tetrahydrofuran-concentrated ammonium hydroxide (50 + 50 + 50 + 5, v/v/v/v) was used. The concentration range was 210 g/spot. The optimized methods proved to be specific and accurate for the analysis of the cited drugs in laboratory-prepared mixtures and dosage forms. The obtained results agreed statistically with those obtained by the reference methods.

Determination of 1,8-Cineole in Eucalyptus Essential Oils by Thin-Layer Chromatography and Densitometry

1995 ◽  
Vol 78 (1) ◽  
pp. 115-117 ◽  
Author(s):  
Carmen Rossini ◽  
Enrique Pandolfi ◽  
Eduardo Dellacassa ◽  
Patrick Moyna
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Essential Oils ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Light Petroleum ◽  
Gas Liquid Chromatography ◽  
Acid Reagent ◽  
Layer Chromatography

Abstract A thin-layer chromatography (TLC) method with scanning is described for evaluation of the 1,8-cineole content of Eucalyptus essential oils. Samples were developed on silica gel G 60 with light petroleum–chloroform (70 + 30) as mobile phase. Color was developed with 4-dimethylaminobenzal-dehyde–sulfuric acid reagent. The absorbance of colored spots was measured at 460 nm. The response was linear between 15 to 75 μg of 1,8-cineole, with a correlation coefficient of 0.9997. The results of each assay were compared with those from gas–liquid chromatography (GLC). The TLC to GLC ratio was constant (0.92).

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