Antibody Testing of Infants Born to Asymptomatic COVID-19 Positive Mothers

2021 ◽  
Vol 16 (10) ◽  
pp. 3-7
Author(s):  
Tonya Robinson ◽  
Nicole Pozzi ◽  
Saeed Jortani
Keyword(s):  
Pregnant Women ◽  
Chart Review ◽  
Retrospective Chart Review ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Antibody Testing ◽  
University Of Louisville ◽  
Specific Igg ◽  
Negative Conclusion ◽  
Future Management

Awareness of SARS-COV-2 IgG may contribute to the management of asymptomatic RT PCR COVID-19 positive pregnant women, their newborns, and future vaccination practices. Objective: Characterize COVID testing results of asymptomatic COVID-19 positive pregnant women and their infants. Our assumption/hypothesis maintained that all infants born to asymptomatic COVID-19 positive mothers would have detectable SARS-CoV-2 specific IgG. Study Design: Retrospective chart review. Clinical demographics/COVID-19 testing of maternal/infant dyads were reviewed/collected for reporting purposes. Setting: Center for Women and Infants (CWI), University of Louisville Hospital, Louisville, KY Participants: Asymptomatic COVID-19 positive pregnant women/infant dyads admitted to the CWI between June 2020 to February 2021. Results: 36 COVID-19 positive asymptomatic mother/37 infant dyads (one set of twins) reviewed. 38% of the mother/infant dyads were positive for SARS-CoV-2 IgG, while 27% of mother/infant dyads were negative for IgG. A COVID-19 positive mother of twins was IgG negative, but both twins were positive. Two mothers in this study group had developed significant COVID-19 disease at 28w4d gestation and 34w0d gestation. Both required intensive care but recovered, and their pregnancies were maintained until 37w4d and 39w3d gestation, respectively. By the time of delivery, both mothers had negative COVID-19 RT PCR testing, but both infants were positive for SARS-CoV-2 IgG antibodies. COVID-19 RT PCR testing on both of these infants at 24 and 48 hours of age was negative. Conclusion: SARS-CoV-2 IgG is passively transferred to the infant during pregnancy of asymptomatic positive COVID-19 mothers however appears variable and/or possibly based on the ability of IgG detection with current testing. Further investigation of the immune system’s response to the SARS-CoV-2 virus during pregnancy can direct future management/treatment during pregnancy, especially in the wake of vaccination for the virus during pregnancy and emerging variants.

scholarly journals Seroprevalence analysis of SARS-CoV-2 in pregnant women along the first pandemic outbreak and perinatal outcome

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0243029
Author(s):  
Cecilia Villalaín ◽  
Ignacio Herraiz ◽  
Joanna Luczkowiak ◽  
Alfredo Pérez-Rivilla ◽  
María Dolores Folgueira ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Horizontal Transmission ◽  
Perinatal Outcomes ◽  
Study Groups ◽  
Serological Testing ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Serum Samples ◽  
Specific Igg ◽  
Specific Igg Antibodies

Objectives To evaluate the progression of the seroprevalence of SARS-CoV-2 in the pregnant population of the south of Madrid during the first wave of the COVID-19 pandemic. Secondarily we aimed to evaluate maternal and perinatal outcomes. Study design Retrospective cohort study conducted at Hospital Universitario 12 de Octubre during weeks 10 to 19 of 2020, coinciding with the Spanish lockdown. We tested 769 serum samples obtained from routine serological testing during the first and third trimesters of pregnancy for specific IgG anti SARS-CoV-2 RBD and S proteins. RT-PCR tests were performed in suspected cases according to clinical practice. We compared maternal and perinatal outcomes in those with delivered pregnancies (n = 578) according to the presence or absence of specific IgG antibodies. Those with positive IgG were subdivided by the presence or absence of Covid-19 related symptoms at any time and the results of RT-PCR testing if performed. Therefore, we had 4 study groups: G1 (IgG negative), G2 (IgG positive, asymptomatic, RT-PCR testing negative or not done), G3 (IgG positive, symptomatic, RT-PCR testing negative or not done), and G4 (IgG positive, symptomatic, RT-PCR positive). Results Seropositivity increased from 0% to 21.4% (95% CI 11.8–31.0) during the study period, of which 27.9% had an asymptomatic course. Overall outcomes were favorable with a significant increased rate of preterm birth in G4 vs G1 (21.4% vs 6.7%) and cesarean/operative delivery (50% vs 26.9%). Asymptomatic and mild cases did not have differences regarding pregnancy course when compared to seronegative women. There were no documented cases of vertical or horizontal transmission. Conclusion Seroprevalence in pregnant women in southern Madrid went up to 21.4% of which 27.9% had an asymptomatic course. Overall perinatal results were favorable, especially in those asymptomatic.

scholarly journals Pregnant women admitted with urinary tract infections to a public sector hospital in South Africa: Are there lessons to learn?

2016 ◽  
Vol 31 (3) ◽  
pp. 79-83
Author(s):  
Francois Kamgang ◽  
Hopolang Maise ◽  
Jagidesa Moodley
Keyword(s):  
South Africa ◽  
Urinary Tract ◽  
Pregnant Women ◽  
Chart Review ◽  
Retrospective Chart Review ◽  
Public Health Facility ◽  
Public Sector Hospital ◽  
Study Population ◽  
Tract Infections

Background: Urinary tract infection (UTI) is associated with poor maternal and foetal outcomes. There is little information on UTI in pregnancy in South Africa. Objectives: To evaluate the frequency of UTI admissions of pregnant women admitted to a public health facility; and, to describe the outcomes of pregnancies complicated by UTI in our study population. Methods: A retrospective chart review of pregnant women admitted with the diagnosis of UTI during the period of 1 January 2012 to 31 December 2012 was conducted. A midstream urine sample of women admitted with symptoms suggestive of UTI was collected for culture. The diagnosis was confirmed if the culture was positive. The data was analysed using SPSS version 21 and descriptive statistics, viz. percentages, frequency and means were estimated. Results: Of 9,881 admissions, 494 (5%) had a diagnosis of UTI based on clinical features. Sixty had positive cultures and were confirmed as having UTI. Women with UTI had high rates of preterm ruptured membranes (n = 5, 8.3%), preterm deliveries (n = 19, 31.6%), anaemia (n = 26, 43.3%) and renal impairment (n = 4, 6.6%). Two cases of pyelonephritis required admission to an ICU because they developed acute respiratory distress syndrome. The most common pathogen isolated using cultures was Escherichia coli (n = 24, 40%). Only 10% had repeat urine cultures. Conclusion: UTI represents 5% of admissions at the study site. This study highlights the need to improve the quality of care of pregnant women with UTI.

scholarly journals MO877PREVALENCE AND PERSISTENCE OF COVID-19 ANTIBODIES IN NEW YORK CITY HEMODIALYSIS CLINIC

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Ohnmar Thwin ◽  
Nadja Grobe ◽  
Xiaoling (Janice) Ye ◽  
Priscila Preciado Rojas ◽  
Leticia M Tapia Silva ◽  
...  
Keyword(s):  
New York ◽  
New York City ◽  
Confidence Interval ◽  
Clinical Diagnosis ◽  
Phase 1 ◽  
Phase 2 ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Antibody Testing ◽  
Luminescence Signal

Abstract Background and Aims Dialysis patients are at higher risk for severe acute respiratory syndrome coronavirus (SARS-CoV-2) infection. Longevity of antibody response to SARS-CoV-2 infection remains unclear. It is reported that maintenance hemodialysis (MHD) patients can mount an antibody response that is similar in intensity and timing to the non-dialysis population. We aim to investigate the prevalence and persistence of antibodies in hemodialysis patients. Method We measured IgG and IgM antibodies in MHD patients as part of a quality improvement project. Four New York City dialysis clinics participated in this study. Strict policy of RT-PCR testing was implemented in clinics for patients with signs and symptoms of Coronavirus Disease 2019 (COVID-19). Initial antibody testing was done on June 10 and July 13, 2020 (phase 1) and retesting was done for previously positive patients between December 9 and 17, 2020 (phase 2). Upon obtaining verbal consent, 3.5 ml of pre-dialysis blood samples were taken via vascular access. SARS-CoV-2 antibodies were determined using the emergency use authorized Diazyme DZ-Lite SARS-CoV-2 IgM / IgG CLIA assays with 100% sensitivity and 98% specificity. Detection of formed immune-complexes is achieved with N-(4-amino-butyl)-N-ethyl-isoluminol; the luminescence signal is reported as units per ml (AU/ml), values ≥ 1.00 AU/ml are considered as “reactive” and < 1.00 AU/ml as “non-reactive.” Results A total of 429 MHD patients were studied in phase 1. Antibodies were present in 130 (30.3%) and only 55 patients with Covid-19 diagnosis confirmed by RT-PCR test were reactive for IgG antibodies. The time to antibody testing was 73 days (median 77; range 30-111) days. In the phase 2 antibody testing, IgG antibodies were only detected in 47 patients (85.5%) 242 days (median 245, range 204 to 268) after clinical diagnosis of Covid-19. Between the two phases of antibody testing, the luminescence signal declined by 40.9 AU/mL (95% confidence interval 31.5 to 50.3) from 54.1±45.3 to 13.2±20.9 AU/mL (P<0.0001 by paired t-test; Figure 1). In univariate logistic regression, a higher number of days between clinical diagnosis of COVID-19 and the second antibody measurement was associated with a lower seropositivity rate (odds ratio 0.929, 95% confidence interval 0.864 to 0.998, P=0.044). Antibody persistence was not associated with age, gender, race, and ethnicity. Conclusion We observed that about 6 out of 7 MHD patients maintain SARS-CoV-2 antibodies over 6-9 months but there is a significant decline of IgG level. The time between clinical diagnosis and IgG testing was associated with IgG decline. Follow up study to understand antibody dynamics in MHD population is a crucial step once vaccines become available.

scholarly journals SARS-CoV-2 seroprevalence in healthcare workers of dedicated-COVID hospitals and non–COVID hospitals of District Srinagar, Kashmir

2020 ◽  
Author(s):  
Muhammad Salim Khan ◽  
Inaamul Haq ◽  
Mariya Amin Qurieshi ◽  
Sabhiya Majid ◽  
Arif Akbar Bhat ◽  
...  
Keyword(s):  
Healthcare Workers ◽  
High Risk Group ◽  
Infection Prevention And Control ◽  
Institutional Setting ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Serum Samples ◽  
Cross Sectional ◽  
Specific Igg ◽  
Specific Igg Antibodies

AbstractBackground and objectiveSARS-CoV-2 infection poses tremendous challenge to the healthcare system of nations across the globe. Serological testing for SARS-CoV-2 infection in healthcare workers, which form a high-risk group, helps in identifying the burden of hidden infection in an institutional setting.MethodsWe present the results of a cross-sectional serosurvey in healthcare workers from two different hospital settings based on their role in the management of SARS-CoV-2 patients in District Srinagar, Kashmir. In addition to testing for the presence of SARS-CoV-2 specific IgG, we collected information on influenza-like symptoms in the last four weeks and the status of RT-PCR testing. SARS-CoV-2 specific IgG antibodies were detected in serum samples using a sensitive and specific chemiluminescent microparticle immunoassay technology.Interpretation and ConclusionOf 2915 healthcare workers who participated in the study, we analysed data from 2905 healthcare workers. The overall prevalence of SARS-CoV-2 specific IgG antibodies was 2.5% (95% CI 2.0-3.1) in the healthcare workers of District Srinagar. Healthcare workers who had ever worked at a dedicated-COVID hospital had a substantially lower seroprevalence of 0.6% (95% CI: 0.2 - 1.9). Among healthcare workers who had tested positive for RT-PCR, seroprevalence was 27.6% (95% CI: 14.0 - 47.2).The seroprevalence of SARS-CoV-2 infection in healthcare workers of District Srinagar is low, reflecting that a high proportion of healthcare workers are still susceptible to the infection. It is crucial to lay thrust on infection prevention and control activities and standard hygiene practices by the healthcare staff to protect them from acquiring infection within the healthcare setting.

Perinatal outcome of congenital heart disease in a population with high consanguinity

2015 ◽  
Vol 43 (6) ◽  
Author(s):  
Mohamed A. Hamdan ◽  
Fares Chedid ◽  
Gharid N. Bekdache ◽  
Muzibunissa Begam ◽  
Walaa Alsafi ◽  
...  
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Pregnant Women ◽  
Perinatal Outcome ◽  
Congenital Heart ◽  
Chart Review ◽  
Primary Outcome ◽  
Fetal Echocardiography ◽  
Retrospective Chart Review ◽  
High Accuracy

AbstractTo report the perinatal pattern and outcome of fetuses with congenital heart disease (CHD) in consanguineous marriages.Retrospective chart review was performed for fetuses undergoing fetal echocardiography (FE) in our institution. The primary outcome was survival at 28 days after birth.Between 1 January 2005 and 31 December 2010, 1950 pregnant women carrying 2151 fetuses underwent a total of 2828 FEs. CHD was diagnosed in 152 fetuses (7.1%), and perinatal outcome was available in 120, among which 78 (65%) had consanguineous parents. Thirteen fetuses diedJoint FE clinics detect most CHD with high accuracy. Consanguinity contributes to a higher prevalence of fetal cardiac and non-cardiac malformations. The presence of extracardiac anomalies is associated with an increase in perinatal mortality.

scholarly journals Severe and critical COVID-19 in pregnancy: A case series from Montreal

2021 ◽  
pp. 1753495X2199021
Author(s):  
Marie-Julie Trahan ◽  
Isabelle Malhamé ◽  
Cristina Mitric ◽  
Camille Simard ◽  
Jed Lipes ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Chart Review ◽  
Case Series ◽  
Retrospective Chart Review ◽  
Supplemental Oxygen ◽  
Careful Consideration ◽  
Non Invasive ◽  
Individualized Approach ◽  
Obstetric Management ◽  
Supplemental Oxygen Therapy

Background Optimal obstetric management for women with coronavirus disease (COVID-19) is not known. We describe the management of six pregnant women requiring in-hospital care for severe COVID-19. Methods A retrospective chart review was conducted to identify pregnant women who tested positive for Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) between 15 March and 30 June 2020. A subset of women meeting criteria for severe COVID-19 was included. Results Four women required non-invasive supplemental oxygen therapy and two required mechanical ventilation. Four women were discharged from hospital undelivered and two required preterm delivery. One woman had a pulmonary embolism, and two required re-admission for worsening symptoms. Conclusion Management of pregnant women with severe COVID-19 is complex and should involve multidisciplinary expertise. Avoiding early delivery may be a safe option. We recommend an individualized approach to care, including careful consideration of the expected risks and benefits of expectant obstetric management versus delivery.

Electroencephalographic Findings in Pediatric Patients with Anti-N-Methyl-D-Aspartate Receptor Encephalitis: The San Diego Experience

2020 ◽  
Author(s):  
Aliya Frederick ◽  
Jennifer H. Yang ◽  
Natalie Guido-Estrada ◽  
Jose Soria-Lopez ◽  
Shifteh Sattar
Keyword(s):  
Early Diagnosis ◽  
Chart Review ◽  
Pediatric Patients ◽  
Retrospective Chart Review ◽  
Nrem Sleep ◽  
Inclusion Criteria ◽  
Antibody Testing ◽  
Early Management ◽  
Aspartate Receptor ◽  
Nmdar Encephalitis

AbstractDiagnosing anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis clinically can be challenging. There is a growing interest in identifying specific electroencephalographic features to help guide early management. A retrospective chart review was conducted of pediatric patients admitted to Rady Children's Hospital between January 1, 2010 and April 1, 2017. We included patients with the diagnosis of encephalitis who underwent continuous video electroencephalogram (VEEG) for at least 12 hours, and presented with less than 14 days of symptoms. We compared the electroencephalographic features of non-rapid eye movement (NREM) sleep between patients with antibody confirmed anti-NMDAR encephalitis and patients with encephalitis from other etiologies. We identified seven patients who met our inclusion criteria, five of whom were diagnosed with anti-NMDAR encephalitis. Four of the five patients had a significant reduction in NREM sleep, while one patient had increased NREM sleep associated with clinical catatonia and hypersomnolence. Sleep was preserved in the two cases of nonimmune mediated encephalitis. Our results suggest that a prolonged VEEG to capture sleep coupled with clinical features can aid in early diagnosis and treatment of anti-NMDAR encephalitis, often before confirmatory antibody testing is available.

Prevalence and Avidity of Human Herpesvirus-6 Specific IgG Antibodies in Pregnant Women in Hungary

2006 ◽  
Vol 53 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Ibolya Stiller ◽  
Rozália Pusztai ◽  
Erzsébet Sombor ◽  
L. Orosz ◽  
A. Pál ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Human Herpesvirus ◽  
Igg Antibodies ◽  
Human Herpesvirus 6 ◽  
Specific Igg ◽  
Herpesvirus 6 ◽  
Specific Igg Antibodies

scholarly journals Asymptomatic Bacteriuria among Women Attending the Antenatal Clinics in Jeddah, Western Region of Saudi Arabia

2016 ◽  
Vol 23 (2) ◽  
pp. 39-47
Author(s):  
Hanan M. Ali Shamrani
Keyword(s):  
Pregnant Women ◽  
Chart Review ◽  
Asymptomatic Bacteriuria ◽  
Retrospective Chart Review ◽  
Improve Patient Care ◽  
University Hospital ◽  
Western Region ◽  
King Abdulaziz University ◽  
Antenatal Clinics ◽  
Improve Patient

This retrospective chart review was conducted to determine the proportion of pregnant women with asymptomatic bacteriuria who were followed up at the antenatal clinics of King Abdulaziz University Hospital, Jeddah between January 2007 and January 2012. Clinical and paraclinical data were recorded for 337 women in their last trimester of pregnancy. The prevalence of asymptomatic bacteriuria in the cohort was 31.26%. The most frequently isolated bacteria included Escherichia coli and Klebsiella pneumoniae in 30.39% and 10.78% of the cases, respectively. In most cases, the isolated bacteria were sensitive to ampicillin (11.57%), amoxicillin (10.38%), and amikacin (5.63%). Most bacteria were resistant to nitrofurantoin (21.06%), tetracycline (21.06%) and cefixime (20.77%). The most common prescribed medications included multivitamins (n = 40; 11.87%), vitamin B12 (n = 47; 13.95%), folic acid (n = 45; 13.35%), and antibiotics (n = 43; 12.75%). Overall, it is possible that pregnant women with asymptomatic bacteriuria are undertreated at our institution. Gaps in the management of these patients should be identified to improve patient care.

scholarly journals Co-infection of Dengue, Zika and Chikungunya in a group of pregnant women from Tuxtla Gutiérrez, Chiapas: Preliminary data. 2019

2020 ◽  
Vol 14 (12) ◽  
pp. e0008880
Author(s):  
Leticia Eligio-García ◽  
María del Pilar Crisóstomo-Vázquez ◽  
María de Lourdes Caballero-García ◽  
Mariana Soria-Guerrero ◽  
Jorge Fernando Méndez–Galván ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Preliminary Data ◽  
Surveillance System ◽  
Health Problems ◽  
The State ◽  
Epidemiological Surveillance ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Endemic State ◽  
Epidemiological Surveillance System

Introduction Dengue, Zika and Chikungunya are RNA Arboviruses present in some areas of Mexico, mainly in the endemic state of Chiapas that is characterized by presence of the vector that transmit them and an ecology that favors high transmission. According to the national epidemiological surveillance system, Dengue has intensified since 2018 and outbreaks continue in various states while for Zika and Chikungunya a decrease in cases has been reported in recent years. The main objective of this study was to determine the incidence of Dengue, Zika and Chikungunya infections during pregnancy in the state of Chiapas. Principal findings The presence of previous and current infections and coinfections diagnosed by molecular (RT-PCR) and immunological (ELISA for IgG determination) techniques indicates a wide circulation of viruses in asymptomatic people, specifically in pregnant women showing that silent infections in dry season contributes to the preservation of viruses. Conclusions From 136 studied samples, 27.7% tested positive for DENV, 8% for ZIKV and 24.1% for CHIKV by RTPCR and the values of IgG in sera show that 83.9% were positive for IgG antibodies against DENV, 65% against ZIKV and 59.1% against CHIKV. Results demonstrated presence of ZIKV and CHIKV, not detected by the epidemiological surveillance system, so the importance of establishing proactive epidemiological systems more strict, especially because these infections in pregnant women can cause severe health problems for newborn children.

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