Thermal behaviour of organic radicals and paramagnetic centres in chert: A case study of Bergeracois brown chert, Dordogne, France

In order to successfully understand the complex evolution of prehistoric societies, archaeologists require absolute dating tools, which are not only accurate but also widely applicable. Thermoluminescence (TL) dating is one such approach that has been successfully used to establish a general chronological framework for prehistoric sites and is particularly suited for use on heated lithic artefacts. Experiments conducted in this study have clearly shown the applicability of Electron Spin Resonance (ESR) isothermal modelling in combination with TL dating to constrain firing temperature. This expands the potential application for TL dating to include artefacts treated at low firing temperatures. The present study shows potential in terms of precision and accuracy for framing the “equivalent firing temperature”. At the same time, the comparison of the TL signal with the lattice-defects and aluminium centres invigorate the use of ESR dating on heated flint, especially with samples that have received low thermal treatment. The presence of organic matter in large quantity raises concern on the pyrolysis effect on the luminescence signal; however, the use of ESR isothermal and isochronal modelling could potentially lead to the ability to overcome current interferences of the organic radicals within the dating signal of TL.

Improving the Stability of Protein–Protein Interaction Assay FlimPIA Using a Thermostabilized Firefly Luciferase

The protein–protein interaction assay is a key technology in various fields, being applicable in drug screening as well as in diagnosis and inspection, wherein the stability of assays is important. In a previous study, we developed a unique protein–protein interaction assay “FlimPIA” based on the functional complementation of mutant firefly luciferases (Fluc). The catalytic step of Fluc was divided into two half steps: D-luciferin was adenylated in the first step, while adenylated luciferin was oxidized in the second step. We constructed two mutants of Fluc from Photinus pyralis (Ppy); one mutant named Donor is defective in the second half reaction, while the other mutant named Acceptor exhibited low activity in the first half reaction. To date, Ppy has been used in the system; however, its thermostability is low. In this study, to improve the stability of the system, we applied Fluc from thermostabilized Luciola lateralis to FlimPIA. We screened suitable mutants as probes for FlimPIA and obtained Acceptor and Donor candidates. We detected the interaction of FKBP12-FRB with FlimPIA using these candidates. Furthermore, after the incubation of the probes at 37°C for 1 h, the luminescence signal of the new system was 2.4-fold higher than that of the previous system, showing significant improvement in the stability of the assay.

Luminol Doped Silica-Polymer Sensor for Portable Organic Amino Nitrogen and Ammonium Determination in Water

We propose a portable sensor, obtained by embedding luminol into the tetraethylorthosilicate/trietoxymethylsilane (TEOS/MTEOS) composite, for the quantitative determination of organic amino nitrogen and ammonium in water with the goal of achieving low levels of concentration. The method is based on the reaction between amino nitrogen compounds and hypochlorite to produce chloramino derivatives. Then, the remaining hypochlorite reacts with luminol sensor by producing a luminescence signal, which was measured by using a portable luminometer, being inversely proportional to nitrogen concentration. The liberation of the luminol from sensor is higher than 90% and the sensor is stable for at least a week at room temperature. This portable method was successfully validated and applied to the analysis of several real waters: fountain, river transition, lagoon, and seawater with recovery values between 92% and 112%, which indicated that the matrix effect was absent. The achieved limit of detection was around 10 µg·L−1, expressed as N. This sensor allows in situ monitoring owing to its simplicity, rapidity, and portability.

Improved Mechanical, Anti-UV Irradiation, and Imparted Luminescence Properties of Cyanate Ester Resin/Unzipped Multiwalled Carbon Nanotubes/Europium Nanocomposites

Cyanate ester resin (CER) is an excellent thermal stable polymer. However, its mechanical properties are not appropriate for its application, with brittle weakness, and it has poor functional properties, such as luminescence. This work innovatively combines the luminescence property and the improved mechanical properties with the inherent thermal property of cyanate ester. A novel nanocomposite, CER/uMWCNTs/Eu, with multi-functional properties, has been prepared. The results show that with the addition of 0.1 wt.% of uMWCNTs to the resin, the flexural strength and tensile strength increased 59.3% and 49.3%, respectively. As the curing process of the CER progresses, the injected luminescence signal becomes luminescence behind the visible (FBV). The luminescence intensity of CER/uMWCNTs/Eu was much stronger than that of CER/MWCNTs/Eu, and the luminescence lifetime of CER/MWCNTs/Eu and CER/uMWCNTs/Eu was 8.61 μs and 186.39 μs, respectively. FBV exhibited great potential in the embedment of photon quantum information. Therefore, it can be predicted that CER/uMWCNTs/Eu composites will not only have a wide range of applications in sensing, detection, and other aspects, but will also exhibit great potential in the embedding of photon quantum information.

MO877PREVALENCE AND PERSISTENCE OF COVID-19 ANTIBODIES IN NEW YORK CITY HEMODIALYSIS CLINIC

Background and Aims Dialysis patients are at higher risk for severe acute respiratory syndrome coronavirus (SARS-CoV-2) infection. Longevity of antibody response to SARS-CoV-2 infection remains unclear. It is reported that maintenance hemodialysis (MHD) patients can mount an antibody response that is similar in intensity and timing to the non-dialysis population. We aim to investigate the prevalence and persistence of antibodies in hemodialysis patients. Method We measured IgG and IgM antibodies in MHD patients as part of a quality improvement project. Four New York City dialysis clinics participated in this study. Strict policy of RT-PCR testing was implemented in clinics for patients with signs and symptoms of Coronavirus Disease 2019 (COVID-19). Initial antibody testing was done on June 10 and July 13, 2020 (phase 1) and retesting was done for previously positive patients between December 9 and 17, 2020 (phase 2). Upon obtaining verbal consent, 3.5 ml of pre-dialysis blood samples were taken via vascular access. SARS-CoV-2 antibodies were determined using the emergency use authorized Diazyme DZ-Lite SARS-CoV-2 IgM / IgG CLIA assays with 100% sensitivity and 98% specificity. Detection of formed immune-complexes is achieved with N-(4-amino-butyl)-N-ethyl-isoluminol; the luminescence signal is reported as units per ml (AU/ml), values ≥ 1.00 AU/ml are considered as “reactive” and < 1.00 AU/ml as “non-reactive.” Results A total of 429 MHD patients were studied in phase 1. Antibodies were present in 130 (30.3%) and only 55 patients with Covid-19 diagnosis confirmed by RT-PCR test were reactive for IgG antibodies. The time to antibody testing was 73 days (median 77; range 30-111) days. In the phase 2 antibody testing, IgG antibodies were only detected in 47 patients (85.5%) 242 days (median 245, range 204 to 268) after clinical diagnosis of Covid-19. Between the two phases of antibody testing, the luminescence signal declined by 40.9 AU/mL (95% confidence interval 31.5 to 50.3) from 54.1±45.3 to 13.2±20.9 AU/mL (P<0.0001 by paired t-test; Figure 1). In univariate logistic regression, a higher number of days between clinical diagnosis of COVID-19 and the second antibody measurement was associated with a lower seropositivity rate (odds ratio 0.929, 95% confidence interval 0.864 to 0.998, P=0.044). Antibody persistence was not associated with age, gender, race, and ethnicity. Conclusion We observed that about 6 out of 7 MHD patients maintain SARS-CoV-2 antibodies over 6-9 months but there is a significant decline of IgG level. The time between clinical diagnosis and IgG testing was associated with IgG decline. Follow up study to understand antibody dynamics in MHD population is a crucial step once vaccines become available.

Ze-Qi-Tang Formula Induces Granulocytic Myeloid-Derived Suppressor Cell Apoptosis via STAT3/S100A9/Bcl-2/Caspase-3 Signaling to Prolong the Survival of Mice with Orthotopic Lung Cancer

Non-small-cell lung cancer (NSCLC) remains the most common malignancy with the highest morbidity and mortality worldwide. In our previous study, we found that a classic traditional Chinese medicine (TCM) formula Ze-Qi-Tang (ZQT), which has been used in the treatment of respiratory diseases for thousands of years, could directly inhibit the growth of human NSCLC cells via the p53 signaling pathway. In this study, we explored the immunomodulatory functions of ZQT. We found that ZQT significantly prolonged the survival of orthotopic lung cancer model mice by modulating the tumor microenvironment (TME). ZQT remarkably reduced the number of MDSCs (especially G-MDSCs) and inhibited their immunosuppressive activity by inducing apoptosis in these cells via the STAT3/S100A9/Bcl-2/caspase-3 signaling pathway. When G-MDSCs were depleted, the survival promotion effect of ZQT and its inhibitory effect on lung luminescence signal disappeared in tumor-bearing mice. This is the first study to illustrate the immunomodulatory effect of ZQT in NSCLC and the underlying molecular mechanism.

Combining drone-derived digital elevation models and single grain luminescence data of modern analogue deposits to reveal sediment transport pathways and geomorphic change in washover fans

<p>The chronostratigraphy of coastal sedimentary records such as washover fans or beach-ridge sequences may be used to reconstruct storm chronologies on centennial to millennial time scales. However, modern analogues are crucial for interpretations of depositional processes and for reducing uncertainty in evaluating the typically complex chronostratigraphic architecture of these landforms. Such a modern analogue was provided by category 3 tropical cyclone (TC) Olwyn in 2015, which caused a significant storm surge in the Gulf of Exmouth, Western Australia, and which activated large washover fans located in the southwestern part of the Gulf. Pre- and post-TC Olwyn geomorphological surveys and high-resolution drone-derived topographical data of one of these washover fans document a detailed history of erosion and deposition during the event. The modern analogue deposits provided an excellent opportunity to evaluate the use of luminescence-based proxies (luminescence inventories) including quartz single-grain age distributions and associated remnant ages, as well as quartz and feldspar luminescence signal comparisons for tracing event-related sediment source environments and understanding transport processes (May et al., 2020). Sediments deposited during Olwyn show a systematic relationship between luminescence characteristics and washover fan position. Seaward and central washover sections are indicated by well-bleached deposits due to the beach as the dominant source and/or long transport distances across the fan. Lateral washover deposits, in contrast, are characterised by rather local source areas and short transport distances, resulting in higher remnant ages of 70-140 a. This data shows that the combination of sediment source environments and sediment transport length across the fan represents the main control in resetting the luminescence signal and enabling reliable depositional ages to be calculated. It documents the benefit of investigating luminescence inventories when establishing chronologies from complex sedimentary records, thereby demanding a careful consideration of local processes and source areas when interpreting sedimentary TC records.</p><p><em>May, S. M., Callow, J. N., Brill, D., Hoffmeister, D., & May, J.-H. (2020). Revealing sediment transport pathways and geomorphic change in washover fans by combining drone-derived digital elevation models and single grain luminescence data. Journal of Geophysical Research: Earth Surface, 125, e2020JF005792. https://doi.org/10.1029/2020JF005792</em></p>

Un-mixing the effect of post-depositional tillage turbation on OSL age-depth data through measurements and numerical simulations

<p>Agricultural systems are subject to severe land degradation, because anthropogenic erosion processes, such as tillage erosion, substantially increase erosion rates compared to natural settings. Optically Stimulated Luminescence (OSL) dating is often used to measure the age of depositional layers to quantify rates of landscape change. OSL dating in agricultural systems is however challenging, because the deposits are reworked in the tillage layer even long after their moment of deposition. This post-depositional mixing resets the built-up luminescence signal, which causes an offset between the apparent OSL ages and the actual deposition ages.</p><p>In this study we illustrate the effect of post-depositional mixing on geochronological OSL age-depth data from northeastern Germany and we developed tools to un-mix depositional and post-depositional ages. We analyzed 32 OSL samples from five locations in a kettle hole to reconstruct spatial and temporal deposition patterns. We were able to correct our chronologies for post-depositional mixing by tillage by accounting for (pre-)historical plough regimes. Next to these empirical data, we also modified a Soil-Landscape Evolution Model called Lorica to numerically simulate the effect of post-depositional mixing on depositional ages. This combination of measurements and simulations enabled us to constrain the spatial and temporal effects of post-depositional mixing on OSL age-depth data more accurately. This is an important step towards getting a better grip on the dynamics of agricultural landscapes including the associated dates and rates.</p>