Dynamics of minor T-lymphocyte subpopulations in patients undergoing kidney transplantation

Objective: to study the dynamics of the indicators of CD3+CD4+CD8+double-positive and CD3+CD4-CD8- double-negative T-lymphocytes in patients who underwent kidney transplantation. Material and methods. Three groups were formed out of 197 allograft recipients. PGF group consisted of patients with primary satisfactory graft function. PGD group - with primary graft dysfunction. GR group - with primary graft dysfunction and histologically confirmed graft rejection. We studied the CD3+CD4+CD8+ (DP) and CD3+CD4-CD8- (DN) T-lymphocyte levels before the transplantation, and on the 1st, 3rd, 7th, 30th, and 90th days after the transplantation. Results. Within a month after the transplantation we noted a decrease in the relative DN T-lymphocyte level in the PGF group, while in the PGD and GR groups this indicator significantly increased. By the 90th day, the count of DN T-lymphocytes had remained unchanged in the PGD group, while there had been a statistically significant increase of this subpopulation in the PGF group. The absolute counts of DN T-lymphocytes in the PGF group on the 1st and 7th days were lower than in the GR group. On the 90th day, there was no statistically significant difference in the absolute number of DN T-lymphocytes in the recipient groups. In all the groups, there was a decrease in the number of DP T-lymphocytes on the 1st day, however, in the PGF group, the relative level was significantly higher. This tendency retained for 3 months. There were no statistically significant differences between the PGD and GR groups. The absolute number of DP T-lymphocytes in the PGF group during the entire observation period was significantly higher than in the PGD and GR groups. Conclusion. We noted a decrease in the indicators of DN T-lymphocytes in the PGF group associated with the increase in the DP T-lymphocyte level within the first three months. In the PGD and GR groups, an increase in the DN T-lymphocyte level was revealed due to a decrease in the indicators of DP T-lymphocytes within 90 days after transplantation.

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Performance of the BD FACSPresto near to patient Analyzer in comparison with representative conventional CD4 instruments in Cameroon.

10.21203/rs.2.24614/v1 ◽

2020 ◽

Author(s):

Bertrand Sagnia ◽

Fabrice MBAKOP Ghomsi ◽

Ana Gutierrez ◽

Samuel SOSSO ◽

Rachel KAMGAING ◽

...

Keyword(s):

T Lymphocytes ◽

T Lymphocyte ◽

Statistical Significance ◽

P Value ◽

Resource Constrained ◽

Inform Consent ◽

Remote Areas ◽

Significant Difference ◽

Sensitivity Specificity ◽

Cd4 Testing

Abstract Background In the context of scaling the viral load in resource limited settings, following HIV infected patient’s adults and children with CD4+ T-lymphocyte count still very important in settings where the decentralization of treatment still has some challenges. Effective HIV monitoring in these resource-constrained settings needs affordable and reliable CD4+ T lymphocytes enumeration methods. We investigated the validity of a BD FACSPresto POC which is a dedicated system for enumeration that uses immunofluorescent technologies. In this study, we have assessed the sensitivity, specificity and correlation between most representative flow cytometry instruments present in Cameroon with more than 5000 CD4 T cells tests per year including FACSCalibur, FACSCount, and PIMA POC from Becton dinkinson and ALERE respectively. Methods 268 patients aged from 1 to 72 years old were enrolled and included in the study after inform consent. The BD FACSPresto POC CD4+ T cell technology was placed at CIRCB and operated by technician staff. HIV infected patients were from Chantal BIYA international reference Center (CIRCB), Centre de Sante Catholique de NKOLODOM, Centre de Sante Catholique de BIKOP and CASS de Nkolndongo – Yaounde We compared the accuracy of the BD FACSPresto and three existing reference technologies with more than 5000 tests per year like FACSCalibur, FACSCount and PIMA according to the number of CD4 test done per year and their repartition in the country. Bland – Altman method and correlation analysis were used to estimate mean bias and 95% limits of agreement and to compare the methods, including analysis by subgroup of participant gestational age. In addition sensitivity and specificity were determined. Statistical significance was set at p-value < 0.05 Results The BD FACSPresto POC system has excellent precision, accuracy and linearity for CD4+ T lymphocytes enumeration. Good correlations were obtained between the BD FACSPresto poc system and other single platform methods. Bland–Altman plots showed interchangeability between two machines mean bias BD-FACSPresto vs PIMA= -126,522(-161,221 to -91,822) BD-FACSPresto vs FACSCount= -38,708 (-58,935 to -18,482) and FACSPresto vs FACSCALIBUR= 0,791(-11,908 to 13,491). Mean difference with Absolute CD4+ T-lymphocyte values obtained from the BD FACSPresto system correlated well with PIMA, FACSCount, and FACSCalibur method with R 2 equal to 0.88, 0.92 and 0.968 respectively with P < 0.001 for all. The mean comparison between values obtained from BD FACSPresto with PIMA, FACSCount, and FACSCalibur using paired T test give P=0.17, P=0.5 and P=0.6 respectively meaning that there is no significant differences between values obtained with BD FACSPresto and PIMA, FACSCount or FACSCalibur CD4 enumeration machines. Further analysis revealed close agreement between all the three instruments with no significant difference between the forth methods (P=0.91) Conclusion This BD-FACSPresto POC system is a simple, robust and reliable system for enumeration of absolute and percentage of CD4+ T-lymphocytes especially suitable for remote areas with limited resources. Having one BD-FACSPresto POC system easy to use, should reduce the cost and thus increase and improved access to CD4 testing for HIV infected patients in resource-constrained countries. BD-FACSPresto POC CD4 will enable reduction in patient time and improve the overall quality of ART service count and may improve test access in remote areas. This technology can allow for greater decentralization and wider access to CD4 testing and ART

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Prior Amiodarone Exposure Reduces Tacrolimus Dosing Requirements in Heart Transplant Recipients

Progress in Transplantation ◽

10.1177/1526924819835840 ◽

2019 ◽

Vol 29 (2) ◽

pp. 129-134 ◽

Cited By ~ 3

Author(s):

Nadine T. Breslin ◽

David M. Salerno ◽

Veli K. Topkara ◽

Farhana Latif ◽

Susan Restaino ◽

...

Keyword(s):

Heart Transplant ◽

Organ Transplant ◽

Patient Characteristics ◽

Tacrolimus Concentration ◽

Transplant Recipients ◽

Primary Graft Dysfunction ◽

Graft Dysfunction ◽

Transplant Patients ◽

Significant Difference ◽

And Control

Introduction: Amiodarone use prior to heart transplant is independently associated with a higher rate of severe primary graft dysfunction and in-hospital mortality. Amiodarone may also alter the pharmacokinetics of medications metabolized via cytochrome P450. No data exist regarding the interaction between pretransplant amiodarone and tacrolimus concentrations. Design: Single-center retrospective study of transplant patients between January 1, 2014, and June 30, 2016. A therapeutic tacrolimus concentration was defined as a trough level between 8 and 15 ng/mL for 2 consecutive days. The primary outcome was the tacrolimus therapeutic weight-based dosing requirements (mg/kg/day) for patients receiving amiodarone prior to transplant when compared to those without prior receipt of amiodarone. Secondary outcomes include the incidence of cellular rejection and mortality within 6 months posttransplant. Results: Multi-organ transplant recipients (n = 3), retransplants (n = 9), those who died prior to a therapeutic level (n = 1), and those receiving amiodarone posttransplant (n = 7) were excluded from the analysis. Of the 80 patients included, 34 (42%) received amiodarone prior to transplant. Patient characteristics were similar, with the exception of primary graft dysfunction incidence (38% in amiodarone vs 8.5% in control, P = .001). The median therapeutic dose was 0.1 (interquartile range [IQR]: 0.07-0.12) versus 0.13 (IQR: 0.09-0.17) in the amiodarone and control groups, respectively, ( P < .01). No significant difference in mortality or rejection was noted. Conclusion: Patients receiving amiodarone prior to transplant require a lower weight-based dose of tacrolimus.

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Treatment of primary graft dysfunction after kidney transplantation by renal artery stent

Nephrology Dialysis Transplantation ◽

10.1093/oxfordjournals.ndt.a027047 ◽

1996 ◽

Vol 11 (1) ◽

pp. 208-210 ◽

Cited By ~ 4

Author(s):

B. Krumme ◽

U. Blum ◽

T. Benzing ◽

E. Keller ◽

P. Schollmeyer ◽

...

Keyword(s):

Kidney Transplantation ◽

Renal Artery ◽

Primary Graft Dysfunction ◽

Graft Dysfunction ◽

Renal Artery Stent

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Role of the Transcription Factor LYL-1 in the Adult and Fetal Hematopoietic Stem Cells.

Blood ◽

10.1182/blood.v104.11.2775.2775 ◽

2004 ◽

Vol 104 (11) ◽

pp. 2775-2775

Author(s):

Claude Capron ◽

Catherine Lacout ◽

Yann Lecluse ◽

Isabelle Poullion ◽

Fedor Svinarchouk ◽

...

Keyword(s):

Stem Cells ◽

Transcription Factor ◽

Hematopoietic Stem Cells ◽

Absolute Number ◽

Binding Motif ◽

Hematopoietic Stem ◽

Cell Counts ◽

Significant Difference ◽

The Absolute

Abstract The hematopoietic stem cells (HSC) have the ability to self-renew and to give rise to all blood lineages. These processes occur via a hierarchy of progenitors with progressively more limited differentiation and self-renewal potential and are orchestrated by specialized protein such as transcription factors. LYL-1 protein contains a basic helix-loop-helix DNA binding motif also found in several proteins involved in the control of cellular proliferation and differentiation such as SCL/TAL-1. As LYL-1 shares an 80% homology at the protein level with SCL/TAL-1, we wanted to determine the function of LYL-1 in hematopoiesis and particularly on HSC. For this study, we used knock in lyl-1−/− mice in which exon 4 was replaced by LacZ/Neo cassette. Lyl−/− mice are viable and have normal blood cell counts as well as a normal marrow cellularity. In addition, using a hematopoietic colony forming cells (CFCs) assay, no significant difference was seen in the myeloid CFCs of either lyl-1−/− or lyl-1+/+ BM and FL cells except a 2-fold increase in the absolute number of BFU-E in lyl-1−/− FL as compared to lyl-1+/+ FL. We analyzed more primitive progenitors in details because using Fluorecein Di-beta Galactopyranoside (FDG)-staining assay, we showed that lyl-1 is mainly expressed in primitive Lin− Sca-1+ c-Kit+ cells (LSK) cells from either BM or FL (91 ± 7% and 78 ± 5% of FDG positive cells in lyl-1−/− BM and FL LSK cells, respectively). In addition, analysis of lyl-1−/− and lyl-1+/+ cells revealed a 1.8-fold and 2-fold decrease in the percentage of primitive LSK in BM and FL, respectively, as compared to wild type cells. Furthermore, using the Hoechst 33342 efflux assay, we noticed a significant decrease in the absolute number of more primitive LSK-SP (side population) cells in lyl-1−/− BM as compared to lyl-1+/+ BM cells (52800 ± 5412 cells/femur versus 91080 ± 8475 cells/femur, respectively) suggesting an important role of LYL-1 in the HSC function. In order to confirm this hypothesis, in vivo assays were performed. We observed a 1.5-fold decrease in the lyl-1−/− BM and FL day 12 CFU-S content as compared to lyl-1+/+ cells. Adoptive transfer experiments were subsequently performed using lethally irradiated Ly5.1 mice. Data showed that lyl-1−/− cells from either BM or FL displayed a hematopoietic reconstitution defect in competitive repopulation assays. Indeed, Ly5.1 recipients were injected with a mixture of 5x106 (5:1), 106 (1:1) or 0.5x106 (0.5:1) lyl-1−/− or lyl-1+/+ Ly5.2 expressing cells and 106 competitive BM Ly5.1 expressing cells. All hosts engrafted with lyl-1−/− BM cells shown a significant reduced levels of chimerism (% of circulating Ly5.2+ cells) as compared to hosts engrafted with lyl-1+/+ BM donors (4.3 ± 2.8% (5:1); 7.5 ± 5.5% (1:1); 0.6 ± 0.3% (0.5:1) in lyl-1−/− BM cells versus 66 ± 8% (5:1); 52 ± 9% (1:1); 53 ± 10% (0.5:1) in lyl-1+/+ BM cells) and similar difference was observed with FL donors (45 ± 2% (5:1); 25 ± 5% (1:1); 11 ± 5% (0.5:1) in lyl-1−/− FL cells versus 83 ± 1% (5:1); 70 ± 3% (1:1); 53 ± 6% (0.5:1) in lyl-1+/+ FL cells). This altered defect in HSC was also confirmed using LTC-IC in vitro experiments. Altogether, our results demonstrate an important role of the transcription factor LYL-1 on the maintenance of HSC properties.

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Enrichment of Mononuclear Cells From Cryopreserved Cord Blood Units Using the Purecell” Select System.

Blood ◽

10.1182/blood.v114.22.2159.2159 ◽

2009 ◽

Vol 114 (22) ◽

pp. 2159-2159

Author(s):

Bryan P Spencer ◽

Indreshpal Kaur ◽

Patrick Fong ◽

Safa Karandish ◽

Nirmali Ponweera ◽

...

Keyword(s):

T Cells ◽

Cord Blood ◽

Mononuclear Cells ◽

Cellular Therapy ◽

Standard Procedure ◽

Absolute Number ◽

Common Procedure ◽

Expansion Time ◽

Significant Difference ◽

The Absolute

Abstract Abstract 2159 Poster Board II-136 INTRODUCTION: Many procedures for manufacturing clinical products for cellular therapy involve enrichment of mononuclear cells (MNC). The most common procedure is density gradient separation. Disadvantages of this procedure are low yield of cells especially with cryopreserved products and the open events during processing. We evaluated use of the Purecell” Select System (PALL Medical, NY) for enriching MNC from cryopreserved Cord Blood Units (CBU). METHODS: Initial experiments were performed to optimize the system for recoveries of total nucleated cells (TNC), MNC, neutrophils, lymphocytes, monocytes and CD34+/CD3+ cells. We evaluated thaw/predilute/filter vs thaw/filter, starting volumes (30 — 95mls) and three different methods for harvesting cells from the filter (standard method, input bag rinse and harvest port rinse). Once conditions were optimized, cryopreserved CBU were thawed and split into two fractions. One half of the product was diluted and processed on the Purecell” Select System. The other half was washed and ficolled. The MNC fraction was CD3+ enriched using CD3/28 beads and then cultured with rIL-2 (200 units/ml) for 14 days. The absolute number of CD3+ cells post culture, fold expansion and viabilities of these cells were determined. RESULTS: The Purecell” procedure was 15 times faster than the ficoll method. Optimal volume to load onto the filter was 50ml. When MNC were harvested by the three recommended procedures, there was no difference in recoveries of TNC, MNC, CD34+ and CD3+ cells and neutrophils. However, the lymphocyte and monocyte recoveries were higher (p<0.05 and p=0.001) when harvested with Input bag rinse compared to the standard procedure. Monocyte recoveries were also higher with the harvest port rinse (p=0.004) when compared to the standard procedure. The direct comparison studies of the two MNC enrichment systems demonstrated that the Purecell” Select System gave significantly higher recovery of TNC (p= 0.003), MNC (p=0.029), CD34+ cells (p<0.001) and granulocytes (p<0.001). There was no statistical difference in T cell recoveries, however, there was a significant difference in the recovery of T cells after CD3/28 enrichment.. Interestingly, T cells began to proliferate earlier from the PALL system compared to ficoll isolated T cells (day 4 vs day 7). Although the fold expansion was greater for the ficolled prepared cells, the absolute numbers of T cells obtained after 14 days of culture with rIL-2 was greater for the Purecell Select System in all experiments. The viabilities of the cells from both cultures were comparable. CONCLUSIONS: PALL's Purecell” Select System can be used for clinical processing since it is a functionally closed system. The advantage of this system compared to the ficoll method are the reduced time for processing, increase yield in T cells (post processing), the earlier expansion time These benefits result in an increase in absolute number of T cells in post culture. A clinical trial using this system is about to be initiated. Disclosures: Karandish: Pall Medical: Employment. McMannis:Pall Medical: Research Funding.

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Hypothermic Machine Perfusion in DCD Kidney Transplantation: A Single Center Experience

Urologia Internationalis ◽

10.1159/000431025 ◽

2015 ◽

Vol 96 (2) ◽

pp. 148-151 ◽

Cited By ~ 5

Author(s):

Liyu Yao ◽

Honglan Zhou ◽

Yuantao Wang ◽

Gang Wang ◽

Weigang Wang ◽

...

Keyword(s):

Kidney Transplantation ◽

Graft Function ◽

Postoperative Recovery ◽

Donation After Cardiac Death ◽

Machine Perfusion ◽

Transplant Recipients ◽

Kidney Transplant Recipients ◽

Single Center Experience ◽

Hypothermic Machine Perfusion ◽

Significant Difference

Introduction: Donation after cardiac death (DCD) began in 2011 after the program hosted by the First Affiliated Hospital of Sun Yat-sen University in China. The aim of this study is to report on our experience regarding the method of preserving donated kidneys for DCD kidney transplantation. Material and Methods: A total of 37 donors and 73 primary kidney transplant recipients during the period 2011-2014 in the Urology Center of the First Hospital of Jilin University were enrolled in the study. Recipients were assigned to traditional static cold storage (SCS) group and hypothermic machine perfusion (HMP) group based on the preservation environment of donated kidneys after organ harvest. Clinical data were collected for each group. Result: The HMP group had a lower rate of delayed graft function (DGF), better postoperative recovery and kidney function compared with that of SCS group. There is no significant difference in postoperative rejection incidence between the 2 groups. Conclusions: DCD kidneys stored by hypothermic machine contribute to a lower rate of DGF and promoted the rehabilitation progress.

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Spousal and living related kidney transplantation: our center experience

BMC Surgery ◽

10.1186/s12893-021-01447-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Utku Ozgen ◽

Murat Ozban ◽

Onur Birsen ◽

Sevda Yilmaz ◽

Belda Dursun ◽

...

Keyword(s):

Kidney Transplantation ◽

Graft Survival ◽

Graft Function ◽

Donor Group ◽

Donor Kidney ◽

Significant Difference ◽

Related Donor ◽

Living Related ◽

Donor Kidney Transplantation ◽

Living Related Donor

Abstract Background Kidney transplantation is the most preferred type of renal displacement therapy for end stage renal disease (ESRD) patients. More patients developed ESRD. The most important source is the donations from unrelated spouses. In this study, we aimed to compare the transplantation data obtained from the spouses of the patients with the transplantation data obtained from other relatives. Methods The data including 167 living kidney transplantations performed between January 2006 and December 2019 were retrospectively collected. The patients were divided into two groups; spousal donor group (n: 53) and living-related donor group (n: 114). Results There was no significant difference in delayed graft function in both groups. There were no patients with acute rejection proven by biopsy or considered biochemically in the spousal donor group. With regard to 3-year results in the living-related donor group the patient survival rate was 100%, while it was 98.2% in terms of graft survival. Conclusions In conclusion, similar patient and graft survival rates between spousal donor kidney transplantation and living-related kidney transplantation has made spousal donor kidney transplantation, with possible problems in terms of tissue compatibility, an acceptable alternative to donor supply.

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Cellular immunity of rabbits incase of parasite association (Treponema cuniculi and Eimeria sp.)

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet9602 ◽

2019 ◽

Vol 21 (96) ◽

pp. 8-13

Author(s):

Y. V. Duda

Keyword(s):

T Lymphocytes ◽

B Lymphocytes ◽

Cellular Immunity ◽

B Lymphocyte ◽

Absolute Number ◽

Blood Parameters ◽

Control Group ◽

The Absolute ◽

T Helpers ◽

Experimental Group

Despite a huge number of studies, the uniqueness of antiparasitic immunity is so great that there is still insufficient knowledge of the factors contributing to the manifestation of the characteristics of immunity in mixed parasitic diseases of rabbits. Therefore, the question of the influence of the association of pathogens Treponema cuniculi and Eimeria sp. on indicators of cellular immunity of rabbits is relevant. The study was conducted on 59 male rabbits age 3–5 months of the Californian breed, selected by analogy. Animal were separated into two groups: healthy animals (control group) and sick animals (research group). Intensity of invasion was determined by the method of the Mac-Master. It has been established that the level of damage of rabbits by spirochetosis and eimeriosis was, on average, 1155.17 ± 184.87 and 6668.97 ± 284.16 pathogens in 1 g of feces. The count of T- and B-lymphocytes was determined by the method of spontaneous rosette-formation with sheep erythrocytes. Parasitizing the association of pathogens Treponema cuniculi and Eimeria sp. was revealed a high number of leukocytes (1.22 times, P < 0.001), which increased mainly due to lymphocytes, which were 1.45 times higher (P < 0.001), as well as neutrophilic metamyelocytes – 1.48 times (P < 0.05), eosinophils – 1.68 times (P < 0.001) and basophils – 1.57 times (P < 0.001) compared with similar blood parameters of healthy animals. In the blood of sick rabbits, the absolute number of T-lymphocytes (1.56 times, P < 0.001) and B-lymphocytes (3.02 times, P < 0.001) was significantly higher in comparison with a low number of O-lymphocytes (3.46 times, P < 0.001) compared with the control. This indicates the redistribution of lymphocytes to cells that carry T and B lymphocyte receptors on the plasma membrane. The absolute number of T-lymphocytes became high due to T-helpers, which in these animals were higher both in absolute (1.87 times, P < 0.001) and percentage (by 9.18%, P < 0.001) compared to control. Moreover, the percentage of T-suppressors in the blood of rabbits of the experimental group was significantly lower on 5.46% (P < 0.05) compared with the same blood count of healthy animals. Such a redistribution of the T-cell population in the peripheral blood of this group of rabbits led to an increase in the immunoregulatory index by 1.64 times (P < 0.01) than in healthy ones. High IRI and the number of T-active lymphocytes (by 28.23%, P < 0.05) in the blood of rabbits with parasitism of the association of pathogens Treponema cuniculi and Eimeria sp. indicate increased immune system tension.

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THE OPPORTUNITIES OF NON-INVASIVE LIVER GRAFT REJECTION DIAGNOSTICS BY USING TERMINALLY DIFFERENTIATED EFFECTOR CD8+ T-LYMPHOCYTES

Hepatology and Gastroenterology ◽

10.25298/2616-5546-2020-4-2-177-183 ◽

2020 ◽

Vol 4 (2) ◽

pp. 177-183

Author(s):

S. V. Korotkov ◽

◽

V. N. Smolnikova ◽

V. Y. Hrynevich ◽

O. A. Lebed ◽

...

Keyword(s):

Liver Transplantation ◽

T Lymphocytes ◽

Graft Rejection ◽

Absolute Number ◽

Liver Graft ◽

Noninvasive Method ◽

Graft Dysfunction ◽

Post Transplant ◽

Cd8 T Lymphocytes ◽

Immune Mediated

Background. Immune-mediated graft dysfunction with the prevalence of 40% is one of the main problems of modern transplantology. Although percutaneous liver graft biopsy is associated with the development of different complications occurring in 2,2% of cases and can also lead to fatal outcome. Objective – to develop a noninvasive method of graft dysfunction diagnostics in the late post-transplant period using terminally differentiated effector CD8+ T-lymphocytes. Material and methods. There was carried out a single center observational retrospective case-control pilot study, including 45 recipients after orthotopic liver transplantation. According to the postoperative clinical course the patients were stratifed into 2 groups depending on the presence of graft rejection episodes. All patients got immunosuppressive therapy after liver transplantation. Immunophenotypes of the recipients were determined by ﬂow cytometry method. Percutaneous liver graft biopsy was performed in all patients, the results of histological examination were evaluated according to the international Banff schema for grading liver allograft rejection. Results. The results of liver biopsies showed that 14 (31%) out of 45 patients had morphological signs of rejection. The patients with rejection had a reliably higher level of CD8+ Temra cells absolute number (0,23 (0,14;0,38) x 109/l) in comparison to those without rejection (0,09) (0,034;0,16) x 109/l (p=0,034)). The results of ROC-analysis have shown that the most optimal cut-off threshold of CD8+ T-lymphocytes level in immune-mediated graft dysfunction diagnostics in the late post-transplant period is 0,1882x109/l; sensitivity and specifcity in this case being 73,33 (95%; 44,9-92,0) and 96,55 (95%; 82,2-99,4) respectively. Conclusions. The increase of terminally differentiated effector CD8+ T-lymphocytes absolute number has diagnostic importance in patients with immune-mediated graft dysfunction in the late post-transplant period. High sensitivity and specifcity of cut-off threshold of CD8+ Temra lymphocytes absolute number in patients after liver transplantation as well as reliable difference between cell number in patients with normal postoperative period and in patients with immune-mediated graft dysfunction allow considering T-lymphocyte subpopulation as a rejection predictor in the late post-transplant period. The correlation between CD8+ T-lymphocyte absolute number and the results of histological examination makes the former an alternative and, what is more, safe noninvasive method in early diagnostics of liver graft rejection.

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