scholarly journals Cellular immunity of rabbits incase of parasite association (Treponema cuniculi and Eimeria sp.)

2019 ◽  
Vol 21 (96) ◽  
pp. 8-13
Author(s):  
Y. V. Duda
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
B Lymphocyte ◽  
Absolute Number ◽  
Blood Parameters ◽  
Control Group ◽  
The Absolute ◽  
T Helpers ◽  
Experimental Group

Despite a huge number of studies, the uniqueness of antiparasitic immunity is so great that there is still insufficient knowledge of the factors contributing to the manifestation of the characteristics of immunity in mixed parasitic diseases of rabbits. Therefore, the question of the influence of the association of pathogens Treponema cuniculi and Eimeria sp. on indicators of cellular immunity of rabbits is relevant. The study was conducted on 59 male rabbits age 3–5 months of the Californian breed, selected by analogy. Animal were separated into two groups: healthy animals (control group) and sick animals (research group). Intensity of invasion was determined by the method of the Mac-Master. It has been established that the level of damage of rabbits by spirochetosis and eimeriosis was, on average, 1155.17 ± 184.87 and 6668.97 ± 284.16 pathogens in 1 g of feces. The count of T- and B-lymphocytes was determined by the method of spontaneous rosette-formation with sheep erythrocytes. Parasitizing the association of pathogens Treponema cuniculi and Eimeria sp. was revealed a high number of leukocytes (1.22 times, P < 0.001), which increased mainly due to lymphocytes, which were 1.45 times higher (P < 0.001), as well as neutrophilic metamyelocytes – 1.48 times (P < 0.05), eosinophils – 1.68 times (P < 0.001) and basophils – 1.57 times (P < 0.001) compared with similar blood parameters of healthy animals. In the blood of sick rabbits, the absolute number of T-lymphocytes (1.56 times, P < 0.001) and B-lymphocytes (3.02 times, P < 0.001) was significantly higher in comparison with a low number of O-lymphocytes (3.46 times, P < 0.001) compared with the control. This indicates the redistribution of lymphocytes to cells that carry T and B lymphocyte receptors on the plasma membrane. The absolute number of T-lymphocytes became high due to T-helpers, which in these animals were higher both in absolute (1.87 times, P < 0.001) and percentage (by 9.18%, P < 0.001) compared to control. Moreover, the percentage of T-suppressors in the blood of rabbits of the experimental group was significantly lower on 5.46% (P < 0.05) compared with the same blood count of healthy animals. Such a redistribution of the T-cell population in the peripheral blood of this group of rabbits led to an increase in the immunoregulatory index by 1.64 times (P < 0.01) than in healthy ones. High IRI and the number of T-active lymphocytes (by 28.23%, P < 0.05) in the blood of rabbits with parasitism of the association of pathogens Treponema cuniculi and Eimeria sp. indicate increased immune system tension.

scholarly journals Sex differences of subpopulation composition of lymphocytes in the peripheral blood in experimental acute and chronic ulcerative colitis

2020 ◽  
Vol 22 (1) ◽  
pp. 157-164
Author(s):  
O. V. Makarova ◽  
E. A. Postovalova ◽  
Yu. Gao ◽  
M. T. Dobrynina
Keyword(s):  
Sex Differences ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Relative Number ◽  
Absolute Number ◽  
Control Group ◽  
Chronic Colitis ◽  
Acute Colitis ◽  
The Absolute ◽  
T Helpers

We studied sex differences lymphocytes subpopulations of peripheral blood in adult C57Bl/6 mice during acute and chronic colitis, induced with 1% DSS. We measured subpopulations of lymphocytes with flow cytometry. We showed that in the control group the female mice had statistically significantly higher values of the relative number of regulatory and cytotoxic T lymphocytes comparing to the males. During acute colitis the females showed an increase in the relative number of Thelpers and a decrease of cytotoxic Tlymphocytes, which reflects the activation of immune response. The males had a decrease in the absolute number of leukocytes, lymphocytes and cytotoxic and regulatory T lymphocytes, probably because of an increase in migration of these cells to the inflammation locus and local lymph nodes. In chronic colitis the females had a decrease in the absolute number of leukocytes, lymphocytes, T helpers, cytotoxic T lymphocytes and B lymphocytes when comparing with acute colitis. During chronic colitis the males had a decrease in the absolute number of T helpers and B lymphocytes but an increase of regulatory T cells in comparison with the control group; in comparison with acute colitis the males with chronic colitis had higher relative and absolute number of regulatory T cells. The increase of T regulatory lymphocytes is due to an increase in their proliferation rate in the thymus and increase of their migration to the inflammatory locus – the colon. Future clinical studies may be based on these results, which show that the treatment of colitis, especially with immunotropic agents, must take sex differences into account.

T and B Lymphocyte Subpopulations in Children

PEDIATRICS ◽  
1975 ◽  
Vol 55 (2) ◽  
pp. 162-165
Author(s):  
Thomas A. Fleisher ◽  
John R. Luckasen ◽  
Andrej Sabad ◽  
Richard C. Gehrtz ◽  
John H. Kersey
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Peripheral Blood ◽  
B Lymphocyte ◽  
Absolute Number ◽  
Lymphocyte Subpopulations ◽  
Older Children ◽  
The Absolute

T and B lymphocyte subpopulations were examined in the peripheral blood of children using the E and EAC rosette assays. Children under 18 months of age were found to have a decreased percentage of E-binding (T) lymphocytes and an increased percentage of EAC-binding (B) lymphocytes compared to older children (18 months to 10 years) and adults. The absolute number of E-binding and EAC-binding lymphocytes was increased in children under 18 months of age.

scholarly journals Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug

2017 ◽  
Vol 7 (4) ◽  
pp. 536-541 ◽  
Author(s):  
M. Khariv ◽  
B. Gutyj ◽  
N. Ohorodnyuk ◽  
O. Vishchur ◽  
I. Khariv ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
Relative Number ◽  
The Body ◽  
T And B Lymphocytes ◽  
Oxidation Stress ◽  
Liposomal Preparation ◽  
Experimental Group

The results of research on the influence of the elaborated complex immunotropic drug containing butafosfan, interferon, thistle and fat-soluble vitamins A, D3, E in the form of a liposomal emulsion on the activity of T- and B-cell chains of immunity in rats under the conditions of action on the body of oxidative stress are presented. It has been established that the introduction of 50% tetrachloromethane into the rats of the first and second experimental groups, with a dose of 0.25 ml per 100 g of body weight, causes oxidative stress in them which negatively affects the cellular immunity and functional activity of immunocompetent blood cells. Immunosuppressive effects of oxidative stress were manifested by a decrease in the blood of rats in the first and second experimental groups of the number of T- and B-lymphocytes and their regulatory subpopulations mainly on the 2nd and 5th day of the study. At the same time, in the blood of rats of the first experimental group in all research periods a decrease in the relative number of common, active and theophylline-resistant T-lymphocytes, as well as B-lymphocytes was observed with a noticeable increase in the number of their undifferentiated forms. At the same time, the obtained data suggest the positive effect of butafosfan, interferon, thistle and vitamins A, D3, E in the liposomal preparation on the relative amount of T- and B-lymphocytes and on the redistribution of avidity in the direction of strengthening the receptor field of plasma membranes immunocompetent cells. It was found that the normalization on the 2nd day of blood level in the second experimental group of common T-lymphocytes occurred due to the secondary forms of the blood and active T-lymphocytes by changes in the number of low-avid forms. In addition to the indicators characterizing the cellular immunity of rats, the components of the liposomal preparation showed regulatory influence on the humoral link of the immune response. In particular, on the 10th day of research in blood of rats of the second experimental group a tendency towards an increase in the relative number of B-lymphocytes and an increase in the number of cells with low and medium density of receptors was found, which, under the conditions of oxidative stress, indicates an increase in the body's ability to actively synthesize protective antibodies

scholarly journals CLINICAL EFFECTS OF HUMAN IMMUNOGLOBULIN ADMINISTRATION IN WOMEN WITH RHESUS SENSITIZATION AT THE PRE-GRAVID STAGE

2020 ◽  
pp. 126-132
Author(s):  
S. G. Chernievskaya ◽  
N. M. Rozhkovska ◽  
V. G. Marichereda ◽  
T. O. Yermolenko
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
Roc Analysis ◽  
Human Immunoglobulin ◽  
Control Group ◽  
Clinical Group ◽  
Clinical Effects ◽  
T Helper ◽  
T Suppressors

The aim of the study – to evaluate changes in cellular immunity in rhesus-sensitized women in response to IVIG administration and the prognostic effectiveness of a method for the prevention of isoimmunization in the next pregnancy. Materials and Methods. The study was performed on the basis of City Maternity Hospital No. 7" (Odesa) in 2014–2019. 37 rhesus-sensitized women were randomly splited in two clinical groups: main clinical group (n=19) where patients received human immunoglobulin for intravenous administration, and control group (n=18) where patients did not receive IVIG. Results and Discussion. The state of cellular immunity in rhesus-sensitized women is characterized by a moderate decrease in the absolute and relative indices of T-lymphocytes while increasing the number of B-lymphocytes. The NK cell population did not differ from the control group. When analyzing subpopulations of T-lymphocytes, it can be concluded that the number of T-helper cells is increased and the number of T-suppressors is proportionally reduced. These changes explain the increase in the number of B-lymphocytes as a result of increasing antigenic load on cell receptors. In the group of women who received IVIG therapy, the ratio of chances of normalization of cellular immunity was 18.41 (95 % CI 2.62–166.74), T-helper – 14.93 (95 % CI) 2.45–107.8), T-suppressors – 14.57 (95 % CI 2.13 –127.57) and B-lymphocytes – 31.87 (95 % CI 4.1–333.41). According to the ROC analysis, the quality of the statistical model of IVIG application corresponds to AUC = 0.843 (95 % CI 0.689–0.941) According to the ROC analysis, the level of β-lymphocytes in the compared AUC groups = 0.58 (95 % CI 0.405–0.742).

scholarly journals The state of cellular immunity in children with recurrent respiratory diseases

2020 ◽  
pp. 64-74
Author(s):  
O Povorova
Keyword(s):  
T Cells ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
Cd8 T Cells ◽  
Respiratory Diseases ◽  
B Lymphocyte ◽  
Correlation Coefficients ◽  
Cytotoxic Lymphocytes ◽  
T Helper ◽  
T Helpers

In children with recurrent respiratory diseases, deviations from the norm of various parameters of the immunity system were determined in more than 85% of cases. The most part of children with indicators outside the range of reference values of the subpopulations of lymphocytes is determined at the age of 3-6 years. In children, a decrease in B lymphocytes (CD19+), as well as T-cytotoxic lymphocytes (СD3+СD8+), is accompanied by an increase in T cells (CD3+) and T helper cells (CD3+CD4+). The most frequent variants of combined disorders of the parameters of the cellular link of immunity were determined: a decrease in B-lymphocytes and an increase in T-cells and T-helpers - in 40.2% (206 out of 512 children); a decrease in B-lymphocytes and a decrease in T-cytotoxic ones - in 42% (215 of 512). Similar correlations between cellular immunity indicators in frequently ill children of Mogilev and Minsk regions were determined: positive Spearman correlation coefficients between CD3/CD4 and CD3/CD8 T- cells, negative-between CD4/CD8 T- cells. The proportion of children with indices of T- and B-lymphocyte subpopulations within the normal range is higher in children of the Minsk region compared with children in the Mogilev region for all the studied subpopulations of lymphocytes (P<0.05). There were no differences in the leukocyte-T-lymphocyte index between the two groups of children.

Effect of cannabinoid ingestion (in the form of bhang) on the immune system of high school and university students

2004 ◽  
Vol 23 (3) ◽  
pp. 149-156 ◽  
Author(s):  
Mona EL–Gohary ◽  
Manal A Eid
Keyword(s):  
High School ◽  
Immune System ◽  
University Students ◽  
Nk Cells ◽  
B Lymphocytes ◽  
Acid Amide ◽  
Absolute Number ◽  
Control Group ◽  
Complement Protein ◽  
The Absolute

The discovery of cannabinoid receptors in the immune system and a family of endogenous ligands of these receptors provides a basis for understanding the cellular and molecular mechanisms of cannabis–induced immunotoxicity. The present study was conducted on 90 nonsmoker males of high school and university students living in Tanta city of matched age and socioeconomic lifetyle. They were divided into a control group (30 males) and a bhang user group (60 males), which used bhang by eating its sweet juice after boiling with a little water and drying in an oven, ‘fola’. The bhang group was divided equally into two subgroups: subgroup 1 used bhang for 6–24 months (average 199–1.2) and subgroup 2 used bhang for 24 / 36 months (average 319–1.7). The immunotoxic effects of using bhang appeared in the form of a significant decrease in serum immunoglobulins (IgG and IgM), and C3 and C4 complement protein concentrations (P<0.05). In addition, our results demonstrated a significant decrease in the absolute number of functionally different subsets of peripheral blood mononuclear lymphocytes, T and B lymphocytes and natural killer (NK) cells in bhang users as compared to controls (P<0.05). Moreover, the fatty acid amide hydrolase (FAAH) showed significant decrease in bhang users as compared to controls and in subgroup 2 as compared to subgroup 1 (P<0.05), indicating that the decrease in FAAH protein level is closely related to the duration of bhang use. Positive correlations were found between FAAH level and the absolute number of mononuclear cells (T, B lymphocytes and NK cells) among bhang user subgroups. The present study is the first study to report on the effect of bhang on complement proteins and immunoglobulins in humans. Our study revealed that bhang–induced immunotoxicity could be attributed to decrease in FAAH protein.

scholarly journals PSVI-22 Immunity indicators of calves obtained from cows with ketosis during the treatment process

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 199-199
Author(s):  
Sergey Kovalev ◽  
Anastasia Nikitina ◽  
Polina Anipchenko ◽  
Anatoly Stekolnikov ◽  
Pavel Kiselenko ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Humoral Immunity ◽  
Cellular Immunity ◽  
Treatment Process ◽  
Absolute Number ◽  
Relative Content ◽  
Newborn Calves ◽  
Cellular And Humoral Immunity

Abstract The purpose of these studies was to determine the indicators of cellular and humoral immunity of calves. Compared to calves obtained from untreated cows, the number of T-lymphocytes in young calves born from cows treated with Timolin increased the level of T-cells to 32.9%, while using Timosplenin 45%. As a result of the use of immunomodulators in treating cows with ketosis in mothers, the relative content of T-lymphocytes in the blood of calves born from them increased when using the drug Leucogen by 1.7 times, Timosplenin by 2.3 times, Timalin in 2.2 times. The use of Leucogen for cows with ketosis contributed to an increase in the percentage of B cells in the blood of calves born by 23%, Timosplenin 83%, and Timalin 42%. At the same time, the absolute number of B-lymphocytes in the treatment of “Leucogen” increased 1.9 times and reached the level of healthy calves, in the treatment with the preparations “Timosplenin” and “Timalin” - the increase was, respectively, 3.0 and 2.3 times and was higher this indicator in young animals born from healthy calves. Thus, the inclusion of dry immunomodulators in the treatment regimen of patients with ketosis leads not only to normalization of metabolism, but is also accompanied by an increase in the level of cellular immunity factors in calves born from them. Under the influence of “Leucogen,” the percentage of lymphocytes was normalized, whereas with the introduction of “Timalin” and “Timosplenin,” the content of this type of cells in newborn calves even exceeded those in healthy young.

scholarly journals Influence of cadmium on the cellular part of the immune system of young cattle

2020 ◽  
Vol 3 (2) ◽  
pp. 47-52
Author(s):  
Y. Y. Lavryshyn ◽  
B. V. Gutyj ◽  
K. Y. Leskiv ◽  
I. I. Hariv ◽  
L. H. Yevtukh ◽  
...  
Keyword(s):  
Immune System ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cadmium Chloride ◽  
Control Group ◽  
T Suppressors ◽  
Wide Range ◽  
Young Cattle ◽  
The Impact ◽  
Experimental Group

The article presents the results of research on the effect of cadmium load on the body of young cattle. The negative effect of Cadmium on the liver causes a wide range of pathological changes at different levels of its organization. The impact of heavy metals on the immune system is particularly significant, because it performs a leading role in maintaining health and is recognized as one of the most sensitive to adverse factors, even in relatively low concentrations. The aim of the study was to investigate the influence of cadmium on the cellular part of the immune system of young cattle. The research was carried out on 10 bulls of six months of age, Ukrainian black-and-white dairy breed, which were formed into 2 groups of 5 animals each: control and experimental. The bulls of the control group were on a normal diet. Animals of the experimental group were administered cadmium chloride at a dose of 0.04 mg/kg body weight of the animal. It was found that feeding bulls with a diet of cadmium chloride, the number of B-lymphocytes on the 5th day of the experiment was 17.54 ± 0.95 %. The lowest number of B-lymphocytes was on the 20th day of testing – 15.12 ± 0.37 %. The study of the number of T-lymphocytes shows that at the beginning of analysis the number of T-lymphocytes in the blood of animals of the control and experimental groups ranged from 40.70 ± 3.62 and 40.85 ± 2.54 %. Subsequently, the number of T-lymphocytes in the blood of the experimental group began to decline. The lowest number of T-lymphocytes was in the experimental group of animals on the 20th day of the research, compared with the control group, this figure decreased by 3.63 %. The state of immunity of animals under cadmium load significantly depends on the ratio of T-helpers to T-suppressors. It was found that the immunoregulatory index of blood of bulls of the experimental group probably decreased from 10 days of the research. The number of T-helper lymphocytes in the blood of the experimental group of bulls under cadmium loading is probably reduced by 15, 20 and 30 days of testing. A probable increase in the number of T-suppressors was noted in the bull's blood of the experimental group on the 20th day of the research. Immunoglobulins of different classes are crucial among bull’s serum proteins under cadmium loading. The concentration of immunoglobulins in the blood under cadmium load decreased by 15.9 % relative to the control group on the 20th day of analysis.

scholarly journals MORPHOLOGICAL COMPOSITION AND PROFILE ACTIVITY OF T- AND B-LYMPHOCYTES OF BLOOD OF DRY-PROOF COWS WITH THE INTRODUCTION OF VARIOUS DOSES OF TISSUE BIOSTIMULATOR

2020 ◽  
Author(s):  
И.А. ПУШКАРЕВ ◽  
Т.В. Куренинова ◽  
Н.В. Шаньшин ◽  
А.И. Афанасьева
Keyword(s):  
B Lymphocytes ◽  
Physiological Saline ◽  
Optimal Dose ◽  
Absolute Number ◽  
T And B Lymphocytes ◽  
Control Group ◽  
Absolute Amount ◽  
Dry Period ◽  
The Absolute ◽  
Different Doses

В статье приведены результаты исследований, целью которых являлось изучение влияния введения разных доз тканевого биостимулятора на пролиферативную активность Т- и В-систем иммунной системы сухостойных коров. Экспериментальные исследования проведены в 2019 г. в производственных условиях АО «Учхоз Пригородное» Индустриального района г. Барнаула на коровах приобского типа черно-пестрой породы, на последних месяцах стельности. Нами сформированы 4 подопытные группы животных с учетом возраста и молочной продуктивности, предшествовавших сухостойному периоду. Тканевой биостимулятор вводился за 55-60 дней до предполагаемого отела, четырехкратно с интервалом в 14 дней, в разных дозах: коровам опытной группы I – 15 мл/гол., II – 22,5 мл/гол., III – 30 мл./гол. Животным контрольной группы вводили физиологический раствор в дозе 22,5 мл./гол. Опытная партия тканевого биостимулятора изготовлена из субпродуктов и боенских отходов пантовых оленей по запатентованной технологии. В ходе исследований установлено, что введение разных доз тканевого биостимулятора способствует увеличению некоторых гематологических показателей и пролиферативной активности Т- и В-лимфоцитов крови сухостойных коров. Оптимальной дозой применения тканевого биостимулятора является 22,5 мл/гол, что способствует повышению содержания в крови сухостойных коров эритроцитов на 11,1 % (p≤0,05), относительного количества рЕ-РОК – на 7,4 % (p≤0,05), ЕМ-РОК – на 9,6 % (p≤0,01), абсолютного количества бЕ-РОК – на 50,0 % (p≤0,05) и ЕМ-РОК – на 83,0 % (p≤0,01), коэффициента абсолютного числа «индукторов-хелперов» и «киллеров-супрессоров» в крови – на 53,8 % (p≤0,05) относительно показателей, установленных у животных контрольной группы. The article presents the results of studies whose purpose was to study the effect of the introduction of different doses of tissue biostimulant on the prophylactic activity of T- and B-systems of immunity of dry cows. The experiment was carried out in 2019 under the production conditions of Uchkhoz Prigorodnoye JSC in the Industrial District of Barnaul on cows of the acquired type of black-motley breed during the dry period. We have formed 4 experimental groups of animals. In the selection, which took into account the age and milk productivity preceding the dry period. Tissue biostimulant was administered during dead time 55-60 days before the expected calving, four times with an interval of 14 days, in different doses in the experimental I - 15 ml / goal., In II – 22,5 ml / goal, in III - 30 ml / goal. In the control, physiological saline was used at a dose of 22,5 ml / goal. An experimental batch of tissue biostimulator was made from offal and slaughter reindeer waste using a patented technology. In the course of the experiment, it was found that the introduction of different doses of tissue biostimulant increases the proliferative activity of blood T and B lymphocytes of dry cows. However, the most optimal dose for using a tissue biostimulant is 22,5 ml / goal, which contributes to the greatest increase in the blood content of dry cows of erythrocytes by 11,1% (p≤0,05) the relative amount of pE-ROCK by 7,4% (p≤0,05), EM-ROCK by 96% (p≤0,01), the absolute amount of BE-ROCK by 50,0% (p≤0,05) and EM-ROCK by 80,0% (p≤0,01), the largest increase in the absolute number coefficient of “inductors- helpers ”and“ killer-suppressors ”in the blood by 53,8% (p≤0,05), relative to indicators established in animals of the control group.

scholarly journals Correction of cellular immunity with biferon-C in piglets in case of specific prevention of circovirosis and mycoplasmosis

2020 ◽  
Vol 222 ◽  
pp. 02056
Author(s):  
Aleksey Shakhov ◽  
Sergey Shabunin ◽  
Mariya Zheynes
Keyword(s):  
T Cells ◽  
B Lymphocytes ◽  
Adaptive Immunity ◽  
Metabolic Activity ◽  
Cellular Immunity ◽  
Absolute Number ◽  
T Suppressors ◽  
Corrective Effect ◽  
Helper Activity ◽  
T Helpers

The results of studying the effect of biferon-C containing recombinant porcine interferons -alpha and -gamma on cellular nonspecific and adaptive immunity in piglets in case of specific prevention of circovirosis and mycoplasmosis are presented. The corrective effect of the drug on cellular immunity was stated, manifested by an increase in the absorption and functional and metabolic activity of phagocytes, the relative and absolute number of T- and B-lymphocytes, T-cells with helper activity and a lower formation of the level of T-suppressors, an increase in the T-helpers/T-suppressors ratio.

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