Digital analysis and quantitative assessment of the cervical surface
with dysplasia

The investigation aims - a quantitative assessment of cervical surface changes with digital analysis and computer technologies in dysplasia. Colposcopy was made in 90 women from 21 to 52 years (avr. age 33,9±8,13 y.o.) with mild epithelial dysplasia (CIN1), moderate dysplasia (CIN2), severe dysplasia (CIN3). The algorithm detected indicators which provide the cervical dysplasia classification on pre cytological and pre molecular-genetic patients investigations. The outcome of an algorithm was the identification of the cervix surface condition severity by an objective quantification. The cervical dysplasia type (CIN) was classified as IndGV values. The mild dysplasia (CIN1) had IndGV=8,5, moderate dysplasia (CIN2) - IndGV=13, severe dysplasia (CIN3) - IndGV=15,6. The cervical affected surface area (IndInt) equalled 0,17 in CIN1, 0,19 in CIN2, 0,22 in CIN3. A change severity has a direct relation with a grey color value. It demonstrates quantify classification in digital analysis. The algorithm is used in real-time mode and no requires considerable material outlays. This makes it possible to use an algorithm after clinical examination and predict patient management.

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Digital analysis of colposcopic images in papillomavirus infection

Journal of Physics Conference Series ◽

10.1088/1742-6596/2127/1/012063 ◽

2021 ◽

Vol 2127 (1) ◽

pp. 012063

Author(s):

A Dushkin ◽

M Afanasiev ◽

S Afanasiev ◽

T Grishacheva

Keyword(s):

Squamous Cell ◽

Open Source Software ◽

Quantitative Assessment ◽

Surface Condition ◽

Digital Analysis ◽

Clinical Indicator ◽

A Value ◽

Papillomavirus Infection ◽

Objective Quantification ◽

Surface Changes

Abstract Acetowhite epithelium (AWE) is one of the main clinical indicator of papillomavirus infection in squamous cell lesions. AWE has a different intensity in various degree of the cervix papillomavirus infection. The digital approach gives the opportunity to estimate AWE intensity by numeric values. The investigation aim is a quantitative assessment of cervical surface changes in papillomavirus infection with digital analysis and computer technologies. The spread of AWE on the cervical surface area has a value of the cervix papillomavirus infection in squamous cell lesions. These two features provide to create the index intensity (IndInt) and index grey value (IndGV). Open-source software ImageJ was used to analysis of colposcopic images. The 8-bit mode was used for the estimation of the AWE grey value. The algorithm of digital analysis detected indicators that provide the severity of papillomavirus infection. The outcomes of an algorithm were the identification of the cervix surface condition severity by an objective quantification.

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Diagnosis and Management of Cardiomyopathies – A Focus on Genetics, Cardiac Magnetic Resonance and Clinical Features

European Cardiology Review ◽

10.15420/ecr.2011.7.3.225 ◽

2011 ◽

Vol 7 (3) ◽

pp. 225

Author(s):

Gianfranco Sinagra ◽

Michele Moretti ◽

Giancarlo Vitrella ◽

Marco Merlo ◽

Rossana Bussani ◽

...

Keyword(s):

Clinical Practice ◽

Cardiac Magnetic Resonance ◽

Magnetic Resonance ◽

Imaging Techniques ◽

Molecular Genetic ◽

Molecular Genetic Analysis ◽

Routine Clinical Practice ◽

Clinical Approach ◽

Diagnosis And Management ◽

Made In

In recent years, outstanding progress has been made in the diagnosis and treatment of cardiomyopathies. Genetics is emerging as a primary point in the diagnosis and management of these diseases. However, molecular genetic analyses are not yet included in routine clinical practice, mainly because of their elevated costs and execution time. A patient-based and patient-oriented clinical approach, coupled with new imaging techniques such as cardiac magnetic resonance, can be of great help in selecting patients for molecular genetic analysis and is crucial for a better characterisation of these diseases. This article will specifically address clinical, magnetic resonance and genetic aspects of the diagnosis and management of cardiomyopathies.

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Molecular genetic pathways in Parkinson's disease: a review

Clinical Science ◽

10.1042/cs20050106 ◽

2005 ◽

Vol 109 (4) ◽

pp. 355-364 ◽

Cited By ~ 24

Author(s):

Shushant Jain ◽

Nicholas W. Wood ◽

Daniel G. Healy

Keyword(s):

Parkinson’S Disease ◽

Parkinson's Disease ◽

Genetic Basis ◽

Genetic Factors ◽

Molecular Genetic ◽

Mendelian Genetics ◽

Susceptibility Factors ◽

Increased Risk ◽

Major Progress ◽

Made In

Major progress has been made in the last decade in understanding the genetic basis of PD (Parkinson's disease) with five genes unequivocally associated with disease. As a result, multiple pathways have been implicated in the pathogenesis of PD, including proteasome impairment and mitochondrial dysfunction. Although Mendelian genetics has been successful in establishing a genetic predisposition for familial PD, this has not been reiterated in the sporadic form. In fact no genetic factors have been unequivocally associated with increased risk for sporadic PD. The difficulty in identifying susceptibility factors in PD has not only been because of numerous underpowered studies, but we have been unable to dissect out the genetic component in a multifactorial disease. This review aims to summarize the genetic findings within PD.

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Cytokine Release in HR-HPV(+) Women without and with Cervical Dysplasia (CIN II and III) or Carcinoma, Compared with HR-HPV(−) Controls

Mediators of Inflammation ◽

10.1155/2007/24147 ◽

2007 ◽

Vol 2007 ◽

pp. 1-8 ◽

Cited By ~ 16

Author(s):

Aagje G. Bais ◽

Ilse Beckmann ◽

Patricia C. Ewing ◽

Marinus J. C. Eijkemans ◽

Chris J. L. M. Meijer ◽

...

Keyword(s):

Cervical Carcinoma ◽

Whole Blood ◽

Cervical Dysplasia ◽

Blood Cultures ◽

Interleukin 12 ◽

Control Group ◽

Severe Dysplasia ◽

Cytokine Release ◽

Cytokine Network ◽

Cin Iii

Aims. We investigated the effect of HR-HPV infection on the capacity of the cytokine network in whole blood cultures during carcinogenesis of cervical carcinoma.Methods. Thirty-nine women with moderate dysplasia, severe dysplasia, cervical carcinoma, or without dysplasia formed the study group. The control group consisted of 10 HR-HPV-negative women without CIN. Whole blood cultures were stimulated with phytohemagglutinin (PHA) and concentrations of tumour necrosis factorα(TNFα), interferonγ(IFNγ), interleukin 2 (IL-2), interleukin 12 (IL-12), interleukin 4 (IL-4), and interleukin 10 (IL-10) were determined by ELISAs.Results. A significant increase in cytokine release was detected in HR-HPV-positive women without dysplasia. In women with cervical cancer, release of IFNγand IL-12 was of the same magnitude as in HR-HPV-positive women without clinical manifestations. Most Th1-type/Th2-type ratios decreased form CIN II to CIN III, and increased from CIN III to invasive carcinoma.Conclusions. (1) Infection with HR-HPV without expression of cervical dysplasia induces activation of the cytokine network. (2) Increases in ratios of Th1-type to Th2-type cytokines at the stage of cervical carcinoma were found by comparison with stage CIN III. (3) Significant changes in the kinetics of cytokine release to a Th2-type immune response in blood of women with cervical dysplasia occurred progressively from CIN II to CIN III.

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Coincidences between molecular genetic and studbook data of gidrán mare families on the basis of mtDnA

Acta Agraria Debreceniensis ◽

10.34101/actaagrar/65/1880 ◽

2015 ◽

pp. 69-73

Author(s):

Nikolett Sziszkosz ◽

Szilvia Kusza ◽

András Jávor ◽

Sándor Mihók

Keyword(s):

Molecular Genetic ◽

Point Of View ◽

Horse Breed ◽

Bottleneck Effect ◽

Hair Samples ◽

D Loop ◽

Loop Regions ◽

Show Jumping ◽

Mtdna Markers ◽

Made In

The traditional Hungarian horse breed, Gidran has been close to the edge of extinction several times. Despite the multiple bottleneck effect, the breed has retained a part of its genetic variability, and performed prominently in carriage driving and show-jumping competitions. Maintaining of the Gidran breed is important in the point of view of world heritage; because besides Hungary, smaller Gidran populations exist only in Bulgaria and Romania. Taking advantage of the special inheritance features of mtDNA, our study focused on two mtDNA regions of Gidran mares. Altogether, 251 hair samples from various Hungarian studs were examined. The analysis was successfully made in case of 251 samples of the cytochrome b and in case of 246 samples of D-loop regions. Because of the distinct mutation rates of the two mtDNA markers, the number of the haplotypes and the way of grouping samples into haplotypes was different. Our key finding was that most haplotypes may be compatible with mare families of the stud book; however incidental mistakes in stud book have occurred only in a few cases. Our results indicate the importance of the preservation and breeding those mare families, which are molecular genetically more diverse than the others, and are in the edge of extinction.

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Technological Study of Rostov Circular Ceramics (Based on Excavation Materials Found at the Livery Yard in 2013)

10.25681/iaras.2018.978-5-9906508-3-1.61-70 ◽

2019 ◽

Author(s):

О.Л. ШАРГАНОВА ◽

А.Е. ЛЕОНТЬЕВ

Keyword(s):

Direct Relation ◽

Morphological Group ◽

Pottery Technology ◽

Technological Study ◽

Technological Analysis ◽

Made In

В статье представлены результаты технологического анализа круговой керамики XI – первой половины XII вв. из нижних пластов раскопа 2013 г. на Конюшенном дворе в Ростове. Технологическое изучение керамики проводилось по методике А.А. Бобринского. В производстве использовались преимущественно сильнозапесоченные ожелезненные глины с дресвой и органикой. К редким традициям относится применение шамота. Предположительно с импортными сосудами связано использование неожелезненной глины и песка. В результате прямой зависимости между формой венчика и технологией не выявлено. Керамику одной морфологической группы изготавливали, вероятно, разные группы гончаров. The report outlines the results of technological analysis of circular ceramics made in the XI – the first half of the XII centuries from the lower layers of the excavation that took place in 2013 at the Livery yard in Rostov. Technological study of ceramics was held according to A. A. Bobrinsky methodology. Hard oversanded ferruginized clay with grus and organic were generally used for ceramics manufacture. Chamotte as material was used quite rarely. Unferruginized clay and sand were presumably typical for imported vessels. The study revealed no direct relation between rim shape and pottery technology. It is likely that ceramics of the same morphological group was made by different groups of potters.

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Autism Spectrum Disorders in Adults

The Oxford Handbook of Adult Cognitive Disorders ◽

10.1093/oxfordhb/9780190664121.013.17 ◽

2019 ◽

pp. 357-377

Author(s):

Charlotte M. Pretzsch ◽

James L. Findon ◽

Declan G. Murphy

Keyword(s):

Molecular Genetic ◽

Autism Spectrum ◽

Repetitive Behaviors ◽

Pharmacological Treatments ◽

Physical And Mental Health ◽

Spectrum Disorders ◽

New Treatment ◽

The Cost ◽

Core Symptoms ◽

Made In

Autism spectrum disorder (ASD) is a common, pervasive neurodevelopmental condition characterized by social impairments and restricted and repetitive behaviors and interests. Furthermore, frequently co-occurring physical and mental health conditions, including depression, anxiety, and epilepsy, contribute to the substantial burden on affected individuals, their family, and society. Consequently, the cost of ASD to society is greater than that of cancer, stroke, and heart disease combined. The cause of ASD is poorly understood, and there are currently no effective pharmacological treatments for its core symptoms. However, significant progress has been made in elucidating the molecular, genetic, neuroanatomical, and neurochemical associates of ASD, potentially paving the way for new treatment approaches.

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Ecological-evolutionary relationships in Passiflora alata from Rio Grande do Sul, Brazil

Brazilian Journal of Biology ◽

10.1590/s1519-69842006000500006 ◽

2006 ◽

Vol 66 (3) ◽

pp. 809-816 ◽

Cited By ~ 6

Author(s):

P. Koehler-Santos ◽

A. P. Lorenz-Lemke ◽

F. M. Salzano ◽

L. B. Freitas

Keyword(s):

Geographical Distribution ◽

Molecular Genetic ◽

Rio Grande ◽

Genetic Data ◽

Evolutionary Relationships ◽

Rio Grande Do Sul ◽

First Report ◽

Ecological Characteristics ◽

Molecular Genetic Data ◽

Made In

The geographical distribution, ecological characteristics, flowering and fruiting times, and pollinating agents of Passiflora alata are considered and related to molecular genetic data gathered simultaneously. The first report on this species in Rio Grande do Sul was made in 1934, only in cultivated gardens. Approximately 20 years later, however, the species was already classified as efferata (wild) in Porto Alegre's suburbs. The data presented here, together with the DNA investigations, indicate that P. alata is actively colonizing previously unoccupied areas of this region.

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Border Surface Changes on the Edge of the Castings with Nodular Graphite

Materials Science Forum ◽

10.4028/www.scientific.net/msf.473-474.153 ◽

2005 ◽

Vol 473-474 ◽

pp. 153-158

Author(s):

Katalin Szocs ◽

Maria Vaida

Keyword(s):

Chemical Composition ◽

Chemical Effect ◽

Technical Literature ◽

Nodular Iron ◽

Iron Castings ◽

Different Types ◽

Nodular Graphite ◽

Heavy Machine ◽

Made In ◽

Surface Changes

The paper presents the study of the microstructure and ultrasound measurments by proeminent part of the nodular iron castings. The experiment has been made in the Havy Machine Factory in Clausenburg. The crossing speed of the ultasound increases near the edge of the castings. Analising the nodular iron microstructure by smoler castings, there occurs no graphite change. By bigger mouldings the shape of the graphite can change to a vermicular graphite near the edges. The thickness of the transformed stratum is of 0,1-0,3 mm. In the nodular graphite iron are acting the forces in the frontier-surface. After casting the evaporation of the magnezium increases near the edge of the castings. The changes of forces on frontier surface can be caused by the changes of the innere tension, and also by the interacting of moulding materials. Opinions regarding these two effects are contardictory in the technical literature. Our experiments have been made in the Heavy Machine Combinat (C.U.G) in Kolozsvar, on nodular iron containing different ferrit-pearlite proportions. The weight of castings varried between 0,5 kg and 8000 kg. The metalic melt necessary to nodulisation was produced inductive furnaces of 6,3 and 12 tons. Before treatment by magnezium alloy, the chemical composition of the iron melt varried between the following parameters, according to the different types of irons: 3,7-3.9% C, 0,2-1,4% Si, 0,1-1,0% Mn, max. 0,025% S, max. 0,05% P, 0,1-1,4% Cr,%, 0,05-0,3% Mo, 0,2-0,8% Cu, 0,2-2,0% Ni. The globularisation has been made in the Tundich-pot. The work-temperature was between 1450 and 1600 C. We used 1,8 – 2,2% FeSiMg and 0,4-1,6% FeSi for the nodulisation. Our target was the examination of the surface strata on the castings, using for mould mixtures with different chemical effect.

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Class-specific herpes simplex virus antibodies in sera and cervical secretions from patients with cervical neoplasia: a multi-group comparison

Epidemiology and Infection ◽

10.1017/s0950268800067194 ◽

1988 ◽

Vol 100 (3) ◽

pp. 455-465 ◽

Cited By ~ 6

Author(s):

G. E. Dale ◽

R. M. Coleman ◽

J. M. Best ◽

B. B. B. Benetato ◽

N. C. Drew ◽

...

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Cervical Dysplasia ◽

Secretory Iga ◽

Severe Dysplasia ◽

Mild Dysplasia ◽

Serum Igg ◽

Serum Iga ◽

Caucasian Women ◽

Simplex Virus

SummarySerum and cervical secretions were collected from patients with cervical dysplasia, carcinoma-in-situ (CIS), squamous cell carcinoma (cervical SCC), and controls with normal cervices, attending clinics within the West Lambeth Health District, London. Enzyme-linked immunosorbent assays were used to examine cervical secretory IgA (sIgA) and serum IgG and IgA antibodies to herpes simplex virus (HSV). Sexual and demographic factors were considered during data analysis, which involved fitting multiple linear or multiple logistic regressions to HSV antibody levels. Prevalence of sIgA-HSV and levels of serum antibodies to HSV in all groups were compared with those of gynaecology controls. Caucasian women with mild dysplasia had a significantly higher prevalence of sIgA-HSV. Serum IgG levels to HSV (IgG-HSV) were significantly elevated in women with mild dysplasia and severe dysplasia/ClS. Serum IgA levels to HSV1 (IgG-HSV1) were significantly higher in women with cervical SCC (after adjusting for smoking habits) and other genital tumours. Significantly higher levels of serum IgA to HSV2 (IgA-HSV2) were also found among Caucasian women with cervical SCC. The possible role of HSV as a co-factor in cervical carcinogenesis is discussed.

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