scholarly journals Haematology, serum biochemistry and organ weight changes of wistar rats fed processed dehulled jack bean (Canavalia ensiformis)

2020 ◽  
Vol 42 (1) ◽  
pp. 71-78
Author(s):  
H. O. Amusa ◽  
R. A. Kehinde ◽  
R. O. Ayotebi ◽  
O. A. Abu
Keyword(s):  
Blood Cells ◽  
Control Diet ◽  
Serum Biochemistry ◽  
Organ Weight ◽  
Weight Changes ◽  
Jack Bean ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin ◽  
L Value ◽  
Dietary Treatments

A total of fifty Wistar albino rats weighing 50-60g were randomly allocated to five dietary treatments in a completely randomized design to investigate the haematology, serum biochemistry and organ weight changes on diets containing processed dehulled jack bean. Four diets containing 10% crude protein were formulated to contain casein (T1)-control diet, dehulled jack bean soaked in filtrate obtained from 24hr water-steeped whole maize grains (T2), dehulled jack bean soaked in lye solution (T3), dehulled jack bean boiled for 15 minutes (T4) while a protein free diet served as a negative control (T5). The feeding trial lasted for 28days after which four rats per treatments were sampled to evaluate haematological and serum biochemical indices while three rats per treatment were sacrificed by cervical decapitation and the heart, liver, kidneys and the lungs removed and weighed separately. Haemoglobin (Hb), white blood cells (WBC), eosinophil and Mean Corpuscular Haemoglobin Concentration (MCHC) were not affected by dietary treatments while the packed cell volume, red blood cells, lymphocytes, neutrophils, monocytes, Mean Corpuscular Volume (MCV) and Mean Corpuscular Haemoglobin (MCH) were significantly affected (P<0.05) by dietary treatments. Rats on T4 had the highest PCV (47.00%), a d neutrophil (25.67%) values, while rat on control had the highest value of RBC 5.95 (×106/mm3). Rats on T2 had the highest lymphocyte (80.33%) and monocyte (3.50%) values, while the lowest value was for rats T4 (73.25%) and T3 (2.00%). Rats on T3 had the highest MCV (1.57fl) and MCH (0.40pg) values the total protein and alkaline phosphatise (ALP) were similar (P>0.05) for all group, while the albumin, globulin, cholesterol, Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) were influence by dietary treatments (P<0.05). Albumin values ranges from 5.83g/dl to 7.68g/dl with rats on T4 having the highest albumin value (7.68g/dl). Globulin value for rats on T4 is significantly (P<0.05) different from rats on T1, T2, T3, and T5 with rats on T3 having the highest value (3.42). Rats on T1 had the highest Cholesterol (55.37mg/dl) and ALT (17.49IU/L) value, while Rats on T2 had the highest AST (16.13IU/L) value. Kidney weights were similar for all group (P>0.05) while the liver, lungs and heart relative weights were significantly affected (P< 0.05) by dietary treatments. Rats on control diet had the lowest relative weight for liver (3.74), lungs (1.03), kidney (0.92) and heart (0.49). Rats on T3 and T5 had the highest value for heart weight (0.75) and liver weight (5.67)while rats on T4 had the highest value for lungs (1.59).Rats fed dehulled jack bean seed soaked in ogi liquor showed slight improvement on haematological, serum profile and organ weight changes over those fed lye soaked jack bean and boiled for 15minutes. However, all the processing methods were ineffective when compared to the performance of rat fed casein based diets.

scholarly journals Effect of garlic rhizome (Allium sativum) powder supplemented diets on the haematology and serum biochemistry of broiler chickens at starter and finisher phases

2020 ◽  
Vol 46 (1) ◽  
pp. 149-156
Author(s):  
D. N. Onunkwo ◽  
J. Nathaniel ◽  
J. C. Ezike ◽  
W. Amaduruonye ◽  
G. Daniel-Igwe
Keyword(s):  
Blood Cells ◽  
Basal Diet ◽  
Serum Biochemistry ◽  
Blood Profile ◽  
Corpuscular Haemoglobin ◽  
Treatment Groups ◽  
Garlic Powder ◽  
Significant Difference ◽  
Mean Corpuscular Haemoglobin ◽  
Broiler Feed

This study was carried out to evaluate the effect of garlic rhizome powder on blood profile of broiler at different growth phases (starter and finisher phase). One hundred and twenty Abor acre broiler chicks were used for the study and the chicks were randomly distributed into four treatment groups and the treatment groups were then divided into different replicates with each replicate having 10 chicks i.e. 30 chicks in each treatment group. The garlic powder was included at different inclusion levels and the treatments were designed as T1: basal diet with no garlic powder, T2: basal diet supplemented with 0.5% garlic powder T3: basal diet supplemented with 1.0% garlic powder and T4 having 1.5% garlic powder. Starter diet was offered to the birds for the first four weeks of the feeding trial and finisher diet was also provided for the birds within 5th to 8th week. Daily feed intake and weekly body measurement were taken. At the end of the starter phase, blood was collected for haematological and serum biochemistry analysis also blood was collected for blood profile analysis at the seventh (8th) week of the experiment. The blood samples collected were analyzed for haematological  parameters which include haemoglobin, Packed cell volume, Red blood cells, White blood cells, Mean corpuscular haemoglobin, Mean corpuscular volume, Mean corpuscular haemoglobin concentration, heterophils, lymphocytes, monocytes, eosinophils and serum biochemical parameters which include Aspartate aminotransferase, Alanine aminotransferase, Alkaline Phosphate, Cholesterol, Total protein, Albumin, Globulin, Uric acid and Creatinine. Results showed that the effect of feeding graded levels of garlic rhizome powder on the haematological and serum parameters were significant (P<0.05). The cholesterol levels of experimental birds dropped significantly with increasing levels of garlic in the diets. Inclusion of garlic powder on broiler feed had no significant difference (P>0.05) on the creatinine level. Therefore 1.5% inclusion of garlic should be added to broiler feed to enhance production of broiler lean meat with lowest cholesterol.  

scholarly journals Haematological parameters and serum biochemistry of Japanese quail chicks (Coturnixcoturnix japonica) fed raw and processed pigeon pea (Cajanus cajan) seed meal based diets

2020 ◽  
Vol 44 (1) ◽  
pp. 89-99
Author(s):  
A. R. Akintunde ◽  
J. J. Omage ◽  
G. S. Bawa ◽  
P. A. Onimisi ◽  
I. Samuel
Keyword(s):  
Blood Cells ◽  
Cajanus Cajan ◽  
Haemoglobin Concentration ◽  
Serum Biochemistry ◽  
Pigeon Pea ◽  
Haematological Parameters ◽  
Seed Meal ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin ◽  
Pea Seed

An experiment was conducted with 720 one - week old Japanese quail chicks to evaluate the effect of feeding raw and processed pigeon pea (Cajanus cajan) seed meal based diets on the haematology and serum biochemistry of the Japanese quails in a completely randomized design. Birds were allocated to eight treatment groups with three replicates each containing 30 birds per replicate. The control diet (TI) had no pigeon pea seed meal, T2 was raw pigeon pea seed while other diets contained 30% of pigeon pea seed meal processed by soaking (for 24, 48 and 72 hours) (T3 – T5), fermentation for 72 hours(T5), boiling for 60 minutes(T6) and roasting for 30 minutes(T7). Blood samples were collected from the birds through the wing  web vein at the 6th week of the feeding trial. The haematological parameters investigated were packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), white blood cells count (WBC), lymphocytes, neutrophils, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) while serum biochemical components were creatinine, cholesterol, albumin, urea and total protein concentrations. The results obtained in this study showed that all the haematological parameters and serum biochemistry of birds were significantly (P<0.05) influenced by dietary treatments except albumin concentration (g/dL). The packed cell volume, haemoglobin concentration, red blood cells and total protein values were within the range of 41.00 – 52.17 %, 13.63 –17.35g/dl, 6.80 – 8.83 (x 106 /mm3 ) and 5.62 – 7.70 g/dl respectively. The results suggest that raw and processed pigeon pea seed meal (PPSM) possess good dietary protein quality for optimal growth and had no adverse effect on haematological and biochemical profiles of quail chicks at 30 % dietary level of inclusion.

scholarly journals Blood Count and Serum Biochemistry Profile in West African Dwarf Goats Fed Ensiled Mixtures of Cocoa Pod, Cassava Pulp and Acacia Leaf

2021 ◽  
Author(s):  
OLuwatosin Christie Raimi ◽  
Abiodun Adefunmilayo Adeloye
Keyword(s):  
Blood Cell ◽  
Serum Biochemistry ◽  
West African ◽  
Blood Profile ◽  
Corpuscular Haemoglobin ◽  
West African Dwarf ◽  
West African Dwarf Goats ◽  
Cassava Pulp ◽  
Mean Corpuscular Haemoglobin ◽  
Dwarf Goats

Abstract This study investigated the effects of feeding ensiled graded combinations of cocoa pod, cassava pulp and Acacia leaf on haematological and serum biochemical of West African Dwarf goats. In the bid to control environmental pollution, the wastes of cocoa and cassava were applied as alternative feed ingredients for the goat in spite of its low nutrients’ quality and digestibility. A total of Twenty-eight (28) WAD goats (bucks), aged 4 to 5 months with average body weight of 7.00±0.2kg was used in a completely randomized design for a 45 days study to evaluate the blood profiles of goats fed diet combinations (0:60:40) to (60:0:40). The results revealed significant differences in some of the blood parameters measured among the treatments. The red blood cell, packed cell volume and haemoglobin ranged from 4.90-7.87x106/ul, 19.25-26.83% and 7.33-11.15g/L respectively. The concentrations of mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration varied significantly (p < 0.05), white blood cell (5.57-9.75x109L) were comparable and lymphocytes (%) ranged from 43.85-55.67. For serum biochemistry, values obtained for total protein and albumin with sodium followed the same trend while cholesterol, urea, creatinine and glucose followed the same trend. Alanine amino transferase, Aspartate aminotransferase and Alkaline phosphatase varied significantly (p<0.05) among the treatment groups. Since the parameters measured were within the normal range for goats’ blood profile, combinations of cocoa pod, cassava pulp with acacia leaves in the ratio of 0:60:40 to 20:40:40 as browse plants do not have any deleterious effect on the health of the animals.

scholarly journals Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Y. Garba ◽  
E. A. Adeola
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Corpuscular Haemoglobin ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Mean Corpuscular Haemoglobin ◽  
Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

Effects of Thyroid Function on Blood Cell Counts and Red Cell Indices – A Retrospective Study at a Tertiary Care Centre in Mandya, Karnataka

2021 ◽  
Vol 8 (27) ◽  
pp. 2434-2438
Author(s):  
Siddegowda M.S ◽  
Chaithra R ◽  
Shivakumar S ◽  
Maithri C.M
Keyword(s):  
Blood Cell ◽  
Blood Cells ◽  
Thyroid Dysfunction ◽  
Haematological Parameters ◽  
Red Cell ◽  
Corpuscular Haemoglobin ◽  
Significant Difference ◽  
Red Cell Indices ◽  
Mean Corpuscular Haemoglobin ◽  
Rbc Count

BACKGROUND Thyroid hormones play an important role in the regulation and production of red blood cells. Thyroid dysfunction induces different effects on blood cells such as anaemia, erythrocytosis, leucopenia, thrombocytopenia and alteration in red cell indices. In this study, we wanted to compare the changes in haematological parameters of thyroid dysfunction patients with those of euthyroid group. METHODS This was a retrospective study done on 310 individuals by collecting data from the medical records. Later the patients were categorized into hypothyroid (33) thyroid stimulating hormone (TSH > 5.5 μIU/mL), hyperthyroid (19) (TSH < 0.3 μIU/mL) and euthyroid (258) (TSH = 0.3 - 5.5 μIU/ml) groups. The haematological parameters of all these patients were obtained by 5-part automated cell count analyser. Finally, the obtained data was analyzed by statistical package for social sciences (SPSS) software. RESULTS The data obtained from the analysis revealed statistically significant (P < 0.05) difference between hypothyroidism, hyperthyroidism and euthyroidism in mean red blood cell (RBC) count, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), red cell distribution width (RDW), white blood cell (WBC) count and platelet count but the difference was not significant for mean haemoglobin, mean corpuscular haemoglobin concentration (MCHC) (P > 0.05). The mean haemoglobin was lower in hypothyroid patients when compared to euthyroid and hyperthyroid patients. The RBC count (P < 0.007), MCH (P = 0.002) and RDW (P < 0.001) showed statistically significant difference between hypothyroidism and euthyroidism, MCV (P = 0.005) showed statistically significant difference between hyperthyroid and euthyroid groups. CONCLUSIONS In case of patients with abnormal haematological parameters, thyroid hormones evaluation is necessary. KEYWORDS Hypothyroidism, Hyperthyroidism, Haemoglobin, Blood Count, Red Cell Indices

scholarly journals The Haematology and Serum Biochemistry Profile of Adult Japanese Quail (Coturnix coturnix japonica)

2017 ◽  
Vol 9 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Onyinyechukwu A. AGINA ◽  
Wilfred S. EZEMA ◽  
Ezinwa M. IWUOHA
Keyword(s):  
Uric Acid ◽  
Total Cholesterol ◽  
Haemoglobin Concentration ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Serum Biochemistry ◽  
Total Proteins ◽  
Corpuscular Haemoglobin ◽  
Coturnix Coturnix ◽  
Mean Corpuscular Haemoglobin

The present study established the reference values and sex differences in the erythrocytic and serum biochemistry parameters of domestic adult quails (Coturnix coturnix). Ninety five adult birds, comprising of 42 males and 53 female Japanese quails were sampled using a simple random sampling technique. Standard procedures were carried out in all haematology and serum biochemistry determinations. The overall mean for the erythrocytic and serum biochemistry parameters were as follows: packed cell volume (PCV) 43.11%, red blood cell count (RBC) 4.31 × 106/µl, haemoglobin concentration (Hbc) 16.21 g/dl, mean corpuscular volume (MCV) 100.69 fl, mean corpuscular haemoglobin (MCH) 39.17 pg, mean corpuscular haemoglobin concentration (MCHC) 39.35 g/dl, aspartate aminotransferase (AST) 59.99 IU/L, alanine aminotransferase (ALT) 20.85 IU/L, alkaline phosphatase (ALP) 107.54 IU/L, total proteins (TP) 5.19 g/dl, albumin (ALB) 3.25 g/dl, globulin (GLB) 1.94 g/dl, albumin: globulin 1.73, total cholesterol (TCHOL) 146.69 mg/dl, total bilirubin (TBIL) 2.37 mg/dl, uric acid (UA) 16.02 mg/dl and creatinine (CREAT) 0.44 mg/dl. The PCV of the males were significantly higher than that of the females, while the MCH and MCHC of the females were significantly higher than those of the males. The serum total proteins, albumin, globulin, uric acid, creatinine, and total cholesterol values of the female quails were higher than those of the male quails. The present data might be useful to avian specialists and veterinary clinicians, but more research works should be carried out on quails to increase the information data base, especially in the tropics.

scholarly journals INDUCTION OF MACROCYTIC-NORMOCHROMIC ANAEMIA IN Clarias gariepinus –Siluriform- EXPOSED TO SUB-LETHAL CONCENTRATIONS OF 1, 1-DIMETHYL 4, 4-BIPIRIDILLIUM DICHLORIDE (PARAQUAT) UNDER LABPRATORY CONDITIONS

2021 ◽  
Vol 14 (1) ◽  
pp. e3789
Author(s):  
Musa Idi-Ogede Abubakar ◽  
Adeshina Ibrahim
Keyword(s):  
Blood Cells ◽  
Clarias Gariepinus ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Corpuscular Haemoglobin ◽  
Normochromic Anaemia ◽  
Mean Corpuscular Haemoglobin ◽  
L 14 ◽  
Corpuscular Haemoglobin Concentration ◽  
Sublethal Concentrations

Introduction: Macrocytic-normochromic anaemia was induced in Clarias gariepinus at intervals of 1, 7 and 14 days. Experimental fish were exposed to test water separately diluted with sub-lethal concentrations of paraquat of 0, 0.03, 0.05, 0.07 and 0.09mg/L. 14 days. No in-depth work has been carried out on Macrocytic-normochromic anaemia in Nigeria. Objective: to evaluate macrocytic-normochromic anaemia in Clarias gariepinus exposed to sublethal concentrations of paraquat. Methods: Blood were collected at intervals of 1, 7 and 14 days .Results: Blood dyscrasias  was observed with a significant (p<0.05) decrease in haemoglobin, haematocrit, red blood cells, white blood cells, lymphocytes and   monocytes. Mean corpuscular volume (MCV), Mean corpuscular haemoglobin (MCH), Neutrophils, Eosinophil and Basophil increased significantly (p<0.05) with increasing concentrations of the toxicant while Mean corpuscular haemoglobin concentration (MCHC) remained normal. Conclusion: Sublethal concentrations of paraquat induced macrocytic-normochromic anaemia in the exposed Clarias gariepinus.

Influence of cardamom essential oils and seeds on growth performance, blood characteristics and immunity of broilers

2015 ◽  
Vol 55 (5) ◽  
pp. 573 ◽  
Author(s):  
Mosa Omidi ◽  
Kamran Taherpour ◽  
Javad Cheraghi ◽  
Hossein A. Ghasemi
Keyword(s):  
Blood Cell ◽  
Essential Oils ◽  
Growth Performance ◽  
Plasma Cholesterol ◽  
Control Diet ◽  
Blood Cholesterol ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin ◽  
Inclusion Level ◽  
The Impact

This study was conducted to investigate the impact of dietary supplementation of cardamom essential oils (CEO) and powdered cardamom seeds (PCS) on the growth performance, plasma biochemistry, haematological characters, immune response and meat acceptability in broilers. In total, 300 day-old male broilers (Ross 308) were allocated to five treatments with six replicates. Dietary treatments included the basal diet as control, CEO-supplemented diets with an inclusion level of 50 (CEO1) or 100 (CEO2) mg/kg, and PCS-supplemented diets with an inclusion level of 3 (PCS1) or 6 (PCS2) g/kg. Feeding PCS1 diet improved (P < 0.05) bodyweight gain and feed conversion ratio (FCR) of broilers during the grower period (11–28 days). Moreover, broilers fed the CEO2 diet had the lowest (P < 0.05) FCR during the whole growth period (0–42 days). Higher bursa index (P < 0.05) was detected in chickens receiving CEO1 and CEO2 diets; additionally, higher spleen index (P < 0.05) was recorded in the CEO1 group at 42 days of age. Haematological parameters including red blood cell, white blood cell, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration values were not influenced (P > 0.05) by diet. By contrast, CEO1 and CEO2 diets decreased (P < 0.05) the low-density lipoprotein cholesterol (LDL-C) concentrations compared with control diet. Plasma cholesterol level was also lower (P < 0.05) in the CEO1 group at 42 days of age. Sensory evaluation of meat samples indicated no differences (P > 0.05) among treatments for appearance, flavour, texture and overall acceptability. Our results suggest that the CEO2 diet was advantageous compared with control diet with respect to feed efficiency. Moreover, CEO1 had a positive effect on the blood cholesterol profile by decreasing the plasma cholesterol and LDL-C levels.

scholarly journals Haematology of air breathing mud eel, Monopterus cuchia (Hamilton, 1822) of Mymensingh and Kishoreganj districts of Bangladesh

2013 ◽  
Vol 22 (2) ◽  
pp. 127-134
Author(s):  
AFM Nazmus Salehin ◽  
Shankar Chandra Mandal
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Air Breathing ◽  
Corpuscular Haemoglobin ◽  
Significant Difference ◽  
Haematological Profile ◽  
Mean Corpuscular Haemoglobin ◽  
Hb Concentration ◽  
Corpuscular Haemoglobin Concentration

Haematological profile of air breathing mud eel, Monopterus cuchia (Hamilton) from Mymensinghh and Kishoreganj were determined in the present study. Erythrocytes, lymphocytes, monocytes, neutrophils, eosinophils and platelet were evaluated. Significantly higher (p < 0.05) haemoglobin (Hb) concentration was found in air breathing mud eel of Kishoreganj than that of Mymensinghh area. The haematocrit (HCT), erythrocyte sedimentation rate (ESR), total red blood cells (TRBC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), total white blood cells (TWBC), lymphocytes, neutrophils, monocytes, eosinophils did not show any significant difference. Findings of the present study showed little variation of haematological profile in air breathing mud eel sampled from Mymensinghh and Kishoreganj. Dhaka Univ. J. Biol. Sci. 22(2): 127-134, 2013 (July)

scholarly journals Stability of haematological parameters in stored blood samples of rainbow trout Oncorhynchus mykiss (Walbaum, 1792)

2017 ◽  
Vol 62 (No. 7) ◽  
pp. 401-405 ◽  
Author(s):  
F. Fazio ◽  
V. Ferrantelli ◽  
C. Saoca ◽  
G. Giangrosso ◽  
G. Piccione
Keyword(s):  
Rainbow Trout ◽  
Blood Cells ◽  
Storage Time ◽  
Haemoglobin Concentration ◽  
Corpuscular Haemoglobin ◽  
Thrombocyte Count ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Mean Corpuscular Haemoglobin Concentration ◽  
Mean Corpuscular Haemoglobin ◽  
Corpuscular Haemoglobin Concentration

The aim of this study was to investigate the influence of storage time at +4 °C on haematological indicators in rainbow trout Oncorhynchus mykiss (Walbaum, 1792). For this purpose, 60 Italian trout were evaluated and red blood cells, white blood cells, thrombocyte count, haematocrit, haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were determined. After blood collection (T<sub>0</sub>), all samples were immediately analysed using an automatic method to assess the haematological parameters and then divided into two different aliquots and stored at +4 °C. The first aliquot was refrigerated for 168 h (T<sub>1</sub>), the second one for 336 h (T<sub>2</sub>). Statistical analysis (one-way repeated-measures ANOVA) showed a significant effect of storage time (P &lt; 0.05) on red blood cells, thrombocyte count, haematocrit, haemoglobin concentration, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. The obtained data expand our knowledge of haematological evaluation techniques and could contribute to the development of an appropriate method for haematological analysis that is suitable for the evaluation of the health status of wild and farmed fish.

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