scholarly journals Differentiation and modulation of phagocyte activity in murine granuloma after treatment with thymulin 5cH

2021 ◽  
Vol 11 (40) ◽  
pp. 148-148
Author(s):  
Leoni Bonamin ◽  
Cesar Sato ◽  
Fabiana Santana ◽  
Thayna Neves Cardoso ◽  
Cideli Paula Coelho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Lymph Node ◽  
T Lymphocytes ◽  
Regional Lymph Node ◽  
Phenotypic Characterization ◽  
Special Focus ◽  
Thymic Hormone ◽  
Male Adult ◽  
Local Lymph Node ◽  
B1 Cells

In previous studies, it was found that thymulin (thymic hormone), when prepared in homeopathic 5CH dilution, can modulate diseases progress and immune processes, such as virus infection in poultry and experimental tumor development. This project aims to study the immune modulatory mechanisms of thymulin 5CH in granulomatous experimental model induced by BCG in mice, with special focus on the phagocytes differentiation from peritoneal B1 stem cells and on the activity of these cells and lymphocyte cooperation at the injury site. Male adult Balb/c mice were inoculated with BCG into the footpad to induce granuloma and its cells were evaluated by histomorphometric methods (Ziehl-Neelsen staining of macrophages) and flow cytometry. The phenotypic characterization of phagocytes, T and B lymphocytes in the peritoneum and regional lymph node was done. Thus, CD11b (activated phagocytes and B1 cells), CD19 (B1 cells and B2), CD23 (B2 cells), CD5 (B1 cell subtypes and T cells), CD4, CD8 (effective T lymphocytes) and CD25 (regulatory lymphocytes) positive cells were quantified. After 21 days of infection, the differentiation of B1 peritoneal stem cells into phagocytes reached the peak, being higher in thymulin-treated mice (X2, p=0.0001). Simultaneously, the number of infected phagocytes in the lesion decreased (KW, p=0.001), indicating remission of the infection. The number of B1 derived phagocytes, CD4+ and CD8+ T lymphocytes in the local lymph node increased in thymulin-treated mice (X2, p=0.0001), at the same time. No difference was seen regarding to CD25+ cells. The results show that thymulin 5CH treatment is able to improve the granuloma inflammatory process by modulating local and systemic phagocyte differentiation, as well as T cell migration into the local lymph node.

scholarly journals Immunomodulation of Homeopathic Thymulin 5CH in a BCG-Induced Granuloma Model

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
Leoni Villano Bonamin ◽  
Cesar Sato ◽  
Ruggero Zalla Neto ◽  
Graziela Morante ◽  
Thayná Neves Cardoso ◽  
...  
Keyword(s):  
Lymph Node ◽  
Immune Modulation ◽  
B Lymphocyte ◽  
Treg Cells ◽  
Vehicle Control ◽  
Phenotypic Characterization ◽  
Male Adult ◽  
Local Lymph Node ◽  
Lymphocyte Populations

The present study analyzed the immune modulation mechanisms of thymulin 5CH in a granuloma experimental model. Male adult Balb/c mice were inoculated with BCG into the footpad to induce granuloma, which was quantitatively evaluated. The phenotypic characterization of phagocyte, T- and B-lymphocyte populations in the peritoneum, and local lymph node was done by flow cytometry. During all experimental periods, thymulin 5CH and vehicle (control) were givenad libitumto mice, diluted into the drinking water (1.6×10−17 M). After 7 days from inoculation, thymulin-treated mice presented reduction in the number of epithelioid cytokeratine-positive cells (P=0.0001) in the lesion, in relation to young phagocytes. After 21 days, the differentiation of B1 peritoneal stem cells into phagocytes reached the peak, being higher in thymulin-treated mice (P=0.0001). Simultaneously, the score of infected phagocytes in the lesion decreased (P=0.001), and the number of B1-derived phagocytes, CD4+ and CD8+ T lymphocytes in the local lymph node increased in relation to control (P=0.0001). No difference was seen on the CD25+ Treg cells. The results show that thymulin 5CH treatment is able to improve the granuloma inflammatory process and the infection remission, by modulating local and systemic phagocyte differentiation.

Induction of tumor-specific cytotoxic T lymphocytes from regional lymph node lymphocytes of human breast cancer

Breast Cancer ◽  
1998 ◽  
Vol 5 (4) ◽  
pp. 367-373 ◽  
Author(s):  
Ryozo Eifuku ◽  
Ichiro Yoshino ◽  
Satoru Imahayashi ◽  
Hiroshi Fujie ◽  
Mitsuhiro Takenoyama ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Human Breast Cancer ◽  
Regional Lymph Node ◽  
Human Breast

scholarly journals Chronic inflammatory response modulation against Leishmania (L.) amazonensis by homeopathic thymulin and antimonium crudum inBalb/c mice

2021 ◽  
Vol 11 (40) ◽  
pp. 163-163
Author(s):  
Fabiana Rodrigues Santana ◽  
Thayna Neves Cardoso ◽  
Cideli Paula Coelho ◽  
Lika Osugui ◽  
Marcia Dalastra Laurenti ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Immune Modulation ◽  
Experimental Period ◽  
Control Treatment ◽  
Thymic Hormone ◽  
Male Adult ◽  
Disease Evolution ◽  
Chronic Inflammatory Response ◽  
B1 Cells ◽  
The Impact

In previous studies it was found that thymulin 5cH (thymic hormone) can modulate immune processes in several diseases. Additionally, the Antimonium crudum has used in dogs bearing leishmaniosis, according to the similia principle. We studied the inflammatory and immune modulation by thymulin 5CH and Antimonium crudum 30CH treatment in mice experimentally inoculated with Leishmania (L.) amazonensis. Male adult Balb/c mice were inoculated with Leishmania (2x105 promastigotes) into the footpad to induce inflammatory response and peritoneum and spleen cells were evaluated by flow cytometry after 60 days. Animals were divided in 3 groups (n=10): thymulin 5cH, Antimonium crudum 30cH and vehicle /control. Treatment was made in blind, daily, in water/alcohol 30% diluted 1:2500 in drinking water, during all experimental period. CD11b (activated phagocytes and B1 cells), CD19 (B1 cells and B2), CD4 and CD8 (effective T lymphocytes) markers were used to identify immune cells subsets in peritoneal washing fluid and spleen cell suspension. Mice treated with thymulin 5cH presented increase in peritoneal and spleen B1 stem cells (X2=0.0001) and higher CD8+/CD4+ ratio in spleen, regarding to the control. Also, Antimonium crudum 30CH induced a mild increase in B1 cells in peritoneum and spleen ( both X2, p=0.0001). Further histological analysis of the primary lesion will be done in the next step, to elucidate the impact of these findings in the disease evolution. The results show that both treatments stimulate B1 stem cell proliferation and suggest the cooperation of T spleen lymphocytes in the process.

EVALUATION OF CLINICAL AND PARACLINICAL FEATURES IN PATIENTS WITH CARCINOMA OF ORAL CAVITY

2016 ◽  
pp. 56-60
Author(s):  
Van Minh Nguyen ◽  
Hong Loi Nguyen ◽  
Thi Kim Anh Dang
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Oral Cavity ◽  
Tumor Size ◽  
Regional Lymph Node ◽  
Regional Lymph Node Metastasis ◽  
Female Ratio ◽  
Histopathological Grade ◽  
Node Metastasis ◽  
Metastasis Rate

Background: To evaluate the clinical, hystopathologycal features and correlation between lymph node metastasis and hystopathologycal grade in patients with carcinoma of the oral cavity. Materials and Methods: From July 2015 to July 2016, 32 patients with carcinoma of the oral cavity at Hue Central Hospital Results: The most common age group from 51 to 60 years and the male/female ratio was 1.9/1. Tumor were usually observed around the the tongue (40.6%) and oral floor (34.4%). Most of the tumor size is larger than 2 cm diameters (> 80%). The regional lymph node metastasis rate was 43.8% and there was a positive correlation between lymph node metastasis and tumor size (p <0.05). Squamous-cell carcinoma was mainly type of histopathology. Difference between the rate of lymph node metastasis in patient groups with different histopathological grade show no statistical significance (p> 0.05). Conclusion: the greater tumor, the higher regional lymph node metastasis. There is no relationship between the lymph node metastasis rate and histopathological grade of oral carcinoma. Key words: : carcinoma of oral cavity, tumor size, lymph node metastasis, histopathology

scholarly journals MicroRNAs and Stem-like Properties: The Complex Regulation Underlying Stemness Maintenance and Cancer Development

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1074
Author(s):  
Giuseppina Divisato ◽  
Silvia Piscitelli ◽  
Mariantonietta Elia ◽  
Emanuela Cascone ◽  
Silvia Parisi
Keyword(s):  
Stem Cells ◽  
Molecular Mechanisms ◽  
Epithelial Mesenchymal Transition ◽  
Embryonic Stem ◽  
Cell Types ◽  
Cancer Development ◽  
Special Focus ◽  
Self Renewal ◽  
Mesenchymal Transition ◽  
Different Cell Types

Embryonic stem cells (ESCs) have the extraordinary properties to indefinitely proliferate and self-renew in culture to produce different cell progeny through differentiation. This latter process recapitulates embryonic development and requires rounds of the epithelial–mesenchymal transition (EMT). EMT is characterized by the loss of the epithelial features and the acquisition of the typical phenotype of the mesenchymal cells. In pathological conditions, EMT can confer stemness or stem-like phenotypes, playing a role in the tumorigenic process. Cancer stem cells (CSCs) represent a subpopulation, found in the tumor tissues, with stem-like properties such as uncontrolled proliferation, self-renewal, and ability to differentiate into different cell types. ESCs and CSCs share numerous features (pluripotency, self-renewal, expression of stemness genes, and acquisition of epithelial–mesenchymal features), and most of them are under the control of microRNAs (miRNAs). These small molecules have relevant roles during both embryogenesis and cancer development. The aim of this review was to recapitulate molecular mechanisms shared by ESCs and CSCs, with a special focus on the recently identified classes of microRNAs (noncanonical miRNAs, mirtrons, isomiRs, and competitive endogenous miRNAs) and their complex functions during embryogenesis and cancer development.

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