scholarly journals Veterinary and sanitary assessment and disinfection of refrigerator chambers of meat processing enterprises

10.5219/1628 ◽  
2021 ◽  
Vol 15 ◽  
pp. 616-626
Author(s):  
Kateryna Rodionova ◽  
Anatoliy Paliy ◽  
Mariia Кhimych
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Raw Materials ◽  
Quantitative Composition ◽  
Meat Processing ◽  
Bacteriostatic Effect ◽  
Life Activity ◽  
Air Samples ◽  
Deep Freezing ◽  
Processing Plants

The results of microbiological studies of air samples of refrigerating chambers of meat processing enterprises are presented. The quantitative composition of the air microbiota of the chambers of the refrigerating shop was studied. It has been established that the technological regimes for cooling meat in cooled chambers (t = +4 °C) and deep freezing chambers (t = -18 °C and -22 °C) have no bacteriostatic effect on the life activity of mold fungi. The developed disinfecting preparation (hydrogen peroxide (8.0 - 10%), acetic acid (10%), peracetic acid (5.0 - 7.0%), stabilizing additives, water) ensures the destruction of sanitary-indicative microorganisms in cold rooms meat processing plants when applied at a concentration of 0.05% - 60 minutes, 0.1% - 30 minutes, 0.15% - 10 minutes and does not have a toxic effect on meat raw materials that are stored in chambers of the refrigeration shop after disinfection.

Identification of antibiotics in meat. Flow cytometry capabilities

Vsyo o myase ◽  
2020 ◽  
pp. 56-60
Author(s):  
Zayko E.V. ◽  
◽  
Kuznetsova O.A. ◽  
Bataeva D.S. ◽  
Grudistova M.A. ◽  
...  
Keyword(s):  
Raw Materials ◽  
Animal Husbandry ◽  
Flow Cytometer ◽  
Poultry Meat ◽  
Meat Processing ◽  
Beta Lactams ◽  
Antimicrobial Substances ◽  
Chemotherapy Drugs ◽  
Processing Plants ◽  
Meat Samples

The problem of the uncontrolled use of antibiotics currently remains unresolved. Step-by-step monitoring of meat using modern methods will reduce the risk of using contaminated meat raw materials for food production. Qualitative monitoring will identify samples containing residual antimicrobial substances. The use of methods for identifying groups of antibiotics will help narrow the search for antibiotics by expensive chromatographic methods. A study was carried out of beef, pork and poultry meat, which is used in meat processing plants in the production of raw smoked sausages, using two methods. At the first stage, using a qualitative microbiological method, the raw meat was evaluated for the presence of antimicrobial substances, then their group was determined using a NovoCyte flow cytometer. According to the results of a study on a flow cytometer, it was found that out of 10 groups of antibiotics that can be determined by the tested method, the group of lincosamides was not found in all meat samples. The most common groups of chemotherapy drugs in pork were sulfonamides – 29.6 %, tetracycline group – 18.5 % and beta-lactams – 14.8 %, and in beef aminoglycosides – 36.7 %, phenicols – 30 % and beta-lactams – 13.3 %. In poultry meat samples, the most common were sulfonamides – 23.2 %, fenicols – 23.2 %, and beta-lactams – 16 %. Five groups of antibiotics were found in all studied types of meat: fenicols, β-lactams, macrolides, polypeptide antibiotics, and quinolones. This indicates their widespread use in animal husbandry and poultry farming.

scholarly journals Airborne Listeria spp. in the red meat processing industry

2011 ◽  
Vol 29 (No. 4) ◽  
pp. 441-447 ◽  
Author(s):  
M. Dobeic ◽  
E. Kenda ◽  
J. Mičunovič ◽  
I. Zdovc
Keyword(s):  
Red Meat ◽  
Positive Case ◽  
Airborne Bacteria ◽  
Meat Processing ◽  
Processing Industry ◽  
Air Samples ◽  
Mesophilic Bacteria ◽  
Processing Plants ◽  
Carcass Contamination ◽  
Settling Rates

The aim of this study was to determine the potential presence of the airborne Listeria spp. and its correlation with the aerobic mesophilic bacteria and Listeria carcass contamination in three red meat slaughtering and three processing plants. Airborne L. seeligeri and L. innocua were determined using 8 (5.06%, n = 158) air samples taken on the locations characteristic for aerosol generating and in a chilly environment. The positive airborne samples of Listeria spp. were in an insignificant (P > 0.05) relation with the highest airborne bacteria counts. On the carcass, only 1 positive case (0.69%, n = 144) of L. innocua was determined, presumably owing to the low airborne Listeria counts and its unpredictable settling rates. In addition, insignificant (P > 0.05) influences of air moisture and airflow on the airborne Listeria were found. Nevertheles, the methods currently used to determine the airborne Listeria and its relationships to aerosol viable mesophilic bacteria and carcass contamination need to be reconsidered in future investigations.

scholarly journals Identification of Listeria monocytogenes Contamination in a Ready-to-Eat Meat Processing Plant in China

2021 ◽  
Vol 12 ◽  
Author(s):  
Hongzhi Zhang ◽  
Fengxia Que ◽  
Biyao Xu ◽  
Linjun Sun ◽  
Yanqi Zhu ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Raw Materials ◽  
Case Fatality ◽  
Etiologic Agent ◽  
Health Concern ◽  
Plate Count ◽  
Processing Plant ◽  
Meat Processing ◽  
Processing Plants

Listeria monocytogenes is the etiologic agent of listeriosis, which remains a significant public health concern in many countries due to its high case-fatality rate. The constant risk of L. monocytogenes transmission to consumers remains a central challenge in the food production industry. At present, there is very little known about L. monocytogenes contamination in ready-to-eat (RTE) processing plants in China. In this study, L. monocytogenes in an RTE meat processing plant in Shanghai municipality was characterized using pulsed-field gel electrophoresis (PFGE) and whole genome sequencing (WGS). Furthermore, the biofilm formation ability of the pathogen was also tested. Results revealed that L. monocytogenes isolates were present in 12 samples out of the 48 samples investigated. Most of them (66.7%, 8/12) were identified from the processing facilities irrespective of observed hygiene levels of aerobic plate count (APC) and coliforms. Coliforms were present in only one processing area. ST5 (1/2b) isolates were predominant (83.3%, 10/12) and were identified in two dominant pulsotypes (PTs) (three in PT3 and seven in PT4, respectively). Results of the core-genome multi-locus sequence typing (cgMLST) showed that ST5 in three PTs (PT1, PT3, and PT4) had 0–8 alleles, which confirmed that clonal transmission occurred in the RTE meat processing facilities. In addition, the biofilm formation test confirmed that the isolates from the processing facilities could form biofilms, which helped them colonize and facilitate persistence in the environment. These results indicated that common sanitation procedures regularly applied in the processing environment were efficient but not sufficient to remove L. monocytogenes isolates, especially biofilm of L. monocytogenes. Furthermore, the ST5 isolates in this study exhibited 12 alleles with one ST5 clinical isolate, which contributes to the understanding of the potential pathogenic risk that L. monocytogenes in RTE meat processing equipment posed to consumers. Therefore, strong hygienic measures, especially sanitation procedures for biofilms eradication, should be implemented to ensure the safety of raw materials. Meanwhile, continuous surveillance might be vital for the prevention and control of listeriosis caused by L. monocytogenes.

ANALYSIS OF THE EFFECT OF PHAGES ON BACTERIA AND THEIR VIABLE BUT NONCULTURABLE CELLS

2021 ◽  
Vol 1 (3) ◽  
pp. 317-322
Author(s):  
T.A. Karachina ◽  
◽  
A.M. Abdullaeva ◽  
L.P. Blinkova ◽  
Yu.D. Pakhomov ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Raw Materials ◽  
Animal Origin ◽  
Meat Processing ◽  
Viable But Nonculturable ◽  
Chemical Substances ◽  
E Coli ◽  
Processing Plants ◽  
Nonculturable Cells ◽  
Alternative Means

The growth in the use of antibiotic and chemical substances in meat processing plants shows the need to search for alternative means of decontamination, the most promising of which are bacteriophages. However, there are a lot of little-studied questions on their use, one of which is the effect of bacteriophages on viable but nonculturable (VBNC) cells of bacteria. In this regard, the article provides data on the analysis of the activity of commercial bacteriophages in relation to bacteriacontaminants of raw materials of animal origin, in particular E. coli and Salmonella enterica typhimurium, as well as their VBNC-cells.

scholarly journals BIOTECHNOLOGICAL APPROACHES IN PROCESSING OF SECONDARY RAW MATERIALS OF MEAT INDUSTRY

2018 ◽  
Vol 15 (30) ◽  
pp. 717-724
Author(s):  
E. E. KURCHAEVA ◽  
V. I. MANZHESOV ◽  
I. V. MAKSIMOV ◽  
V. L. PASHCHENKO ◽  
S. Y. CHURIKOVA ◽  
...  
Keyword(s):  
Economic Efficiency ◽  
Raw Materials ◽  
Meat Products ◽  
Starter Cultures ◽  
Farm Animals ◽  
Processed Meat ◽  
Industrial Complex ◽  
Meat Industry ◽  
Meat Processing ◽  
Processing Plants

The radical change in the quality of the processed meat raw materials, which does not meet the standards, calls for the necessity to apply biotechnology methods in order to improve the quality and obtain high nutritional and biological value of manufactured products in compliance with the requirements set for functional food. The use of starter cultures to perform biomodification of cattle by-products makes it possible to provide the rationale for the use of low-value secondary meat raw materials in order to expand the manufacturing range and increase the economic efficiency of meat-processing plants. This article aims to, first, review the approaches towards the development of new forms of biologically complete and environmentally safe sources of raw materials to increase the economic efficiency and reduce costs at meat-processing plants of the agro-industrial complex of the Central Black Earth region and, second, to substantiate the selection and purposefully apply bifidobacteria as part of the consortium for biotransformation of proteins of the rumen of farm animals. As a result of the research, the technology of rumen modification was suggested aimed at its further use as part of meat products, primarily, minced convenience meat products. The article data can be used to develop manufacturing formulas at meat-processing plants and also be useful for process engineers and scientists working in the food industry.

scholarly journals OXIDATIVE-ORGANOSOLVENT DELEGIFICATION OF WHEAT STRAW

2020 ◽  
pp. 250-256
Author(s):  
Irina Trembus ◽  
Nina Semenenko
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Wheat Straw ◽  
Raw Materials ◽  
Raw Material ◽  
Cellulose Content ◽  
Regression Equations ◽  
Residual Lignin ◽  
Technological Parameters ◽  
Vegetable Raw Materials

Urgency of the research. Expansion of the raw material base of the pulp and paper industry of Ukraine takes place through the use of alternative types of vegetable raw materials, namely wheat straw, and the improvement of environmentally friendly oxidative-organosolvent technology for the production of straw pulp. Target setting. Methods for the delignification of vegetable raw materials using the oxidant – hydrogen peroxide – are currently available. The disadvantage of these methods is the high consumption of the oxidant, which constitutes 90 % by weight of absolutely dry raw material that significantly increases the cost of technical cellulose. Therefore, it is necessary to improve the technological parameters of the delignification process of wheat straw with hydrogen peroxide in acetic acid. Actual scientific researches and issues analysis. Recent open-access publications have been reviewed, including literature on oxidative-organosolvent delignification methods of plant material using peroxoacids. Uninvestigated parts of general matters defining. Investigation of technological parameters of oxidative-organosolvent delignification for the reduction of oxidant consumption and determination of optimal values of temperature and duration of the delignification process of wheat straw with the mixture of hydrogen peroxide in acetic acid. The research objective. Investigation of straw pulp production method in order to reduce the consumption of cooking reagents while increasing the physical- mechanical properties and yield of the target product. The statement of basic materials. A method of obtaining straw pulp from wheat straw shreddings in acetic acid media with the hydrogen peroxide consumption 50 % by weight of absolutely dry raw material was investigated. The content of the main components in the raw vegetable material was determined. The effect of the delignification conditions, namely the temperature and cooking duration, on the yield of the fibrous semi-finished product, the content of residual lignin and the content of cellulose was studied. It was shown that the increase of temperature from 70 °C to 90 °C and duration of vegetable raw materials processing from 60 to 180 min lead to the decrease in yield by 28 %, the content of residual lignin by 6.5 %, while the cellulose content in the fibrous semi-finished product increased by 21 %. Calculated regression equations adequately described the experimental data and could be used as a mathematical model of the delignification process of wheat straw by hydrogen peroxide in acetic acid media. Optimal values of technological parameters of the wheat straw cooking process were determined using the method of multicriterion optimization of the delignification conditions. Conclusions. Calculated regression equations adequately described the production process of straw cellulose by hydrogen peroxide delignification in acetic acid. Established optimal technological parameters provided obtaining a final product with high quality indicators (yield 78.2%, residual lignin content 3.6%, cellulose content 65.4%, breaking length – 6200 m, punching resistance 210 kPa, tearing resistance – 425 mN, folding endurance 625 n.d.b.).

scholarly journals Express methods for determining the processing of meat by disinfectants during storage and sale

2018 ◽  
Vol 20 (92) ◽  
pp. 24-28
Author(s):  
N. M. Bogatko ◽  
I. V. Yatsenko ◽  
T. I. Fotina
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Potassium Permanganate ◽  
Quality And Safety ◽  
Meat Processing ◽  
Rapid Methods ◽  
Industrial Markets ◽  
Express Methods

The need to improve the control of meat slaughter animals and poultry in their rigging to develop modern express methods that have credibility in the indicators within and 99,4 to 99.9%, and can be used along with other methods to determine their quality and safety in the manufacturing laboratory facilities for meat processing, slaughtering companies and companies with sales and storage of meat (supermarkets, wholesale warehouses, refrigerators and the like), in the state laboratories of veterinary medicine and in laboratories of veterinary-sanitary examination of agro-industrial markets. Despite the fact that the responsibility for the safety and quality of food of animal origin is entrusted to the manufacturer, the protection of consumer health is always under control of the state. To ensure consumers receive safe and high quality meat need to develop new rapid methods for their adequate control. Especially relevant are the research directions for the development of rapid methods to objectively assess the safety and quality of meat of slaughtered animals and poultry during storage, and the implementation or use for the production of meat products. In stores, supermarkets, wholesale bases, the agricultural markets during the implementation of the meat of slaughtered animals and poultry it is often possible to detect the adulteration of meat for the handling of formalin, chlorine, hydrogen peroxide, acetic acid, potassium permanganate for long-term storage and eliminate the signs of damage. Therefore, we have performed experimental studies and developed rapid methods to identify this type of fraud. Developed rapid methods for the determination of adulteration of meat of slaughter animals and poultry with formalin, chlorine, hydrogen peroxide, acetic acid, potassium permanganate are simple to implement and their results specific quality indicators. Therefore, these rapid methods are available us along with other methods for determining the quality and safety of meat slaughtered animals and poultry (determination of organoleptic, the content of amino-ammoniac nitrogen, hydrogen sulfide, ammonia, pH, etc). These methods can be used in determining the safety and quality of meat of slaughtered animals and poultry during storage, and the implementation of laboratories at the facilities of meat processing, supermarkets, wholesale bases, in government veterinary laboratories and laboratories of veterinary-sanitary examination of agro-industrial markets.

scholarly journals PENGARUH KONSENTRASI KATALIS DAN WAKTU REAKSI PADA PEMBUATAN EPOKSI MINYAK GORENG BEKAS

2017 ◽  
Vol 6 (3) ◽  
pp. 28-33
Author(s):  
Yenni Listiana ◽  
Hilde Rosa Tampubolon ◽  
Mersi Suriani Sinaga
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Glacial Acetic Acid ◽  
Catalyst Concentration ◽  
Raw Materials ◽  
Oxirane Oxygen ◽  
Waste Cooking Oil ◽  
Cooking Oil ◽  
Natural Oil ◽  
Oxygen Conversion

Epoxy is produced from an epoxidation of vegetable oil or natural oil with au nsaturated bond. Epoxy can be applied as a stabilizer, plasticizers in polyvinyl chloride (PVC) and can be used as an antioxidant in natural rubber processing, as a surfactant, anti-corrosive additive agent in lubricants and pesticide raw materials. The purpose of this research was to evaluate epoxy production from waste cooking oil. In this research, waste cooking oil was reacted with hexane as solvent, sulfuric acid as catalyst, glacial acetic acid and hydrogen peroxide. The catalyst concentration was varied from 1.5%, 2.1%, 2.5%, 3.1% and 3.5% and the epoxidation time was varied from 60, 120, 180, 240 and 300 min. The results showed that highest epoxy yield was achieved at reaction time of 300 min and 1.5% catalyst. At that condition, the iod number was 0,96 g I2/100 g WCO, oxirane oxygen content was 1.872 and oxirane oxygen conversion was 62.259%.

scholarly journals Listeria monocytogenes Contamination Characteristics in Two Ready-to-Eat Meat Plants From 2019 to 2020 in Shanghai

2021 ◽  
Vol 12 ◽  
Author(s):  
Hongzhi Zhang ◽  
Jing Wang ◽  
Zhaoyu Chang ◽  
Xin Liu ◽  
Weijie Chen ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Raw Materials ◽  
Foodborne Pathogen ◽  
Food Plants ◽  
Source Tracking ◽  
Nucleotide Polymorphisms ◽  
Meat Processing ◽  
Multiple Loci ◽  
Processing Plants ◽  
Allelic Differences

Listeria monocytogenes is a ubiquitous foodborne pathogen that causes listeriosis and is mostly linked to consumption of ready-to-eat (RTE) foods. Lack of hygiene in food processing environments may be a primary reason for contamination by L. monocytogenes isolates. In this study, L. monocytogenes strains isolated from two RTE meat processing plants in the Shanghai municipality, China, were characterized during 2019–2020 using pulsed-field gel electrophoresis and whole-genome sequencing. Results showed that 29 samples (12.2%) out of 239 were positive for L. monocytogenes, with 21 (18.9%) and 8 (6.25%) isolates from plants A and B, respectively. The packaging room at plant A had the most contamination (14, 48.3%; p < 0.05), with a peak occurrence of 76.5% in processing environments. Nineteen L. monocytogenes isolates belonging to the pulsotype (PT) 7 group were indistinguishable (≥ 95.7%). Furthermore, core-genome multiple loci sequencing typing identified up to nine allelic differences, and the closet pairwise differences among these ST5 isolates included 0–16 small nucleotide polymorphisms. Therefore, L. monocytogenes likely persisted at plant A during 2019–2020 with ongoing clone transmission. In contrast, no L. monocytogenes isolates were identified from processing environments at plant B. Most L. monocytogenes isolates were sampled from raw materials (62.5%). Several isolates (ST378, ST8, and ST120) were detected only once in 2020 and were considered as transient isolates. However, three ST121 isolates with the same PT (PT2) were detected in 2020 and should be noted for their stronger survival ability in harsh environments. These results suggest that continuous monitoring, stringent surveillance, and source tracking are crucial to guaranteeing food safety in RTE food plants.

scholarly journals OBTAINING PULP FROM WHEAT STRAW IN A TWO-STAGE SYSTEM “ACETIC ACID – HYDROGEN PEROXIDE – WATER – ETHANOL”

2020 ◽  
pp. 210-221
Author(s):  
Irina Trembus ◽  
◽  
Nina Semenenko ◽  
Keyword(s):  
Hydrogen Peroxide ◽  
Acetic Acid ◽  
Wheat Straw ◽  
Ethyl Alcohol ◽  
Raw Materials ◽  
Cellulose Content ◽  
Technological Parameters ◽  
Plant Raw Materials ◽  
Second Stage ◽  
Vegetable Raw Materials

Urgency of the research. Involvement of secondary raw materials in the form of agricultural waste and development of new ecologically safe ways of delignification of vegetable raw materials to solve problems of environmental pollution.Target setting. Available methods of delignification of vegetable raw materials using oxidant - hydrogen peroxide allow to obtain a fibrous semi-finished product with a high yield, but with strength values of 15-30% lower compared to fibrous semi-finished products obtained bycooking solutions at pH > 7. Therefore, it is necessary to improve existing methods. hydrogen peroxide in acetic acid.Actual scientific researches and issues analysis. The latest available domestic and foreign publications on oxidative-organosolvent methods of delignification of plant raw materials using peroxyacids and alcohols were considered in detail.Uninvestigated parts of general matters defining. Research of technology of oxidative-organosolvent delignification of vegetable raw materials with useof alcohol as a part of a cooking solution and definition of optimum values of technological parameters (temperature and duration) of process of reception of straw fibrous semi-finished products.The research objective. Investigation of technological parameters of straw cellulose production in the system acetic acid-hydrogen peroxide-water-ethyl alcohol in order to increase the physical and mechanical parameters and the yield of the target product.The statement of basic materials.A two-stage method for producing straw pulp from wheat straw at the first stage has been investigated. Delignification was carried out in the “acetic acid-hydrogen peroxide-water” environment at a hydrogen peroxide consumption of 50% of the mass of absolutely dry raw material (a.d.r.m.) and at the second stage of cooking, 40 volume% of ethyl alcohol was added to the cooking solution to replace the same amount of spent cooking solution after the first stage. The content of the main components in the original plant raw materials isdetermined. The influence of delignifica-tion conditions, namely temperature and duration of cooking on the yield of fibrous semi-finished product, the content of re-sidual lignin, cellulose content and strength indicators was studied. It is shown that the temperature increase at the first stage of the process from 50 ºС to 70 ºС, at the second stage from 80 to 100 ºС and the duration of processing of vegetable raw materials from 60 to 120 min. and from 60 to 180 minutes, according to the stages of the delignification process, leads to a decrease in yield by 7.9-18.9%, the residual lignin content by 4.27-9.11%, while the cellulose content in the fibrous semi-finished product increases by 10.1-15.8%. It is investigated that the indicators of mechanical strength of the obtained fibrous semi - finished products increase with increasing temperature and duration of processing of raw materials. Regression equa-tions for each stage of cooking are calculated, which adequately describe the experimentaldata and can be used as a mathe-matical model of the process of delignification of wheat straw with hydrogen peroxide in acetic acid. The method of multicrite-ria optimization of delignification conditions determines the optimal values of technological parameters of the wheat straw cooking process. The lignin-carbohydrate diagram of delignification of vegetable raw materials is offered. The number in which various methods of delignification of wheat straw are located on efficiency is defined.Conclusions. The technology of obtaining straw fibrous semi-finished products in the system "acetic acid-hydrogen per-oxide-water-ethanol" has been developed. Regression equations are calculated that adequately describe the experimental data and can be usedas a mathematical model of the investigated method of obtaining straw fibrous semi-finished product. The optimal technological parameters are established, which ensure the production of the final product with high quality indicators. The lignin-carbohydrate diagram of delignification of wheat straw stalks by different cooking methods is given.

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