scholarly journals Determination of the species specificity of the primers for the detection of chicken and turkey meat by realtime PCR method

10.5219/390 ◽  
2014 ◽  
Vol 8 (1) ◽  
Author(s):  
Lenka Maršálková ◽  
Miloš Mašlej ◽  
Ľubomír Belej ◽  
Jozef Golian ◽  
Radoslav Židek
Keyword(s):  
Species Specificity ◽  
Turkey Meat ◽  
Realtime Pcr ◽  
Pcr Method

scholarly journals Identification of the Trichinella species by PCR method

2012 ◽  
Vol 66 (1-2) ◽  
pp. 41-47
Author(s):  
Sasa Vasilev ◽  
Jelena Cvetkovic ◽  
Ivana Radovic ◽  
Ljiljana Sofronic-Milosavljevic
Keyword(s):  
Human Health ◽  
Health Problem ◽  
Continuous Monitoring ◽  
Human Consumption ◽  
Sylvatic Cycle ◽  
Trichinella Infection ◽  
High Prevalence ◽  
Pcr Method ◽  
Pig Meat

Serbia is country with a high prevalence of Trichinella infection in pigs, which continues to be a serious human health problem. In Serbia, only a few isolates of Trichinella found in pork have been genetically specified to date, and all were proven as T. spiralis. New data shows that in the sylvatic cycle in Serbia, at least in the Belgrade district, more than one Trichinella species co-exist (T. spiralis and T. britovi). Increased awareness of the possible overlap among sylvatic and domestic Trichinella cycles indicates the need for continuous monitoring of Trichinella species circulation and strongly points to the need that all isolates of Trichinella found in meat for human consumption should be subject to a determination of the Trichinella species (due to the risk of transmission of infection with T. britovi to domestic pigs and humans). This is why we examined using PCR the Trichinella larvae found in pig meat that caused a human outbreak (Trichinella infection) in Grocka (Belgrade district) during February 2011. The isolated larvae belonged to T. spiralis.

scholarly journals Histochemical analysis of skeletal muscular tissues of pigs according to genotype MYF4

2008 ◽  
Vol 24 (1-2) ◽  
pp. 117-126
Author(s):  
Vladimir Zimmermann ◽  
V. Kulísek ◽  
A. Copík ◽  
M. Odstrcil ◽  
Ondrej Debrecéni ◽  
...  
Keyword(s):  
Rectus Femoris ◽  
Muscle Fibres ◽  
Fat Cells ◽  
Average Thickness ◽  
Triceps Brachii ◽  
Surface Representation ◽  
Histochemical Analysis ◽  
Pcr Method ◽  
Muscular Tissues

The results of histochemical analysis of three muscles m. triceps brachii (MTB), m. longissimus thoracicus (MLT) and m. rectus femoris (MRF) of two groups of pigs created according to the genotypes MYF 4 are presented. Determination of MYF 4 genotypes was made by PCR method and for histochemical analysis was used 5 animals detected as homozygote MYF 4- AA type and 5 animals of heterozygote genotype myogenin-AB out of the total of 25 individual animals tested. The histochemical analysis proved that homozygotes AA have had bigger fat cells than heterozygotes AB in three studied muscles in average. The size of fat cells in MLT - 41.10?m or 38.50 ?m respectively dominated in both groups of animals. Percentage surface representation of interstitial tissues was higher in the studied muscles of heterozygote MYF 4-AB. The volume of ligaments was the highest in MRF (3.80% or 3.90% respectively) in both groups (myogenin - AA and AB). The average thickness was of three studied muscles muscle fibres higher at homozygote genotype myogenin-AA than in heterozygote myogenin-AB. The thickest fibres in both genotypes were in MRF (88.60 ?m, and 84.72 ?m respectively) and the lowest in MTB (73.30 and 69.40 ?m respectively). The highest values of muscle fibres thickness were detected in ?-White fibres. Their percentage surface representation corresponded to this in all three types of muscles of both studied genotype myogenin groups.

scholarly journals Caries activity test by realtime PCR method and bacterial cultural method

2020 ◽  
Vol 44 (3) ◽  
pp. 126-129
Author(s):  
Joung-Hee Yun ◽  
Ji-Hyeon Park ◽  
Ja-Won Cho ◽  
Sung-Won Kim
Keyword(s):  
Caries Activity ◽  
Realtime Pcr ◽  
Activity Test ◽  
Cultural Method ◽  
Pcr Method

Determination of Specific Class E Immunoglobulins to Bet v 1 Birch Allergen by the Immuno-PCR Method

2018 ◽  
Vol 44 (2) ◽  
pp. 217-224 ◽  
Author(s):  
M. A. Simonova ◽  
V. D. Pivovarov ◽  
D. Yu. Ryazantsev ◽  
M. A. Kostromina ◽  
T. I. Muravieva ◽  
...  
Keyword(s):  
Specific Class ◽  
Bet V 1 ◽  
Pcr Method ◽  
Class E

scholarly journals A new method for rapid detection of Listeria monocytogenes in food

2000 ◽  
Vol 18 (No. 3) ◽  
pp. 103-109 ◽  
Author(s):  
K. Zdeňková ◽  
K. Demnerová ◽  
G. Jeníková ◽  
J. Pazlarová
Keyword(s):  
Listeria Monocytogenes ◽  
Pcr Primers ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Special Importance ◽  
Ground Meat ◽  
Specific Detection ◽  
Gene Encoding ◽  
Pcr Method

Listeria monocytogenes represents serious danger for human health. Thus detection of this pathogen in food, which represents its main means of entry into the organism, is a topic of special importance. The original classic methods for the determination of Listeria monocytogenes are in general laborious and time-consuming procedures. In order to address this issue we developed a new rapid method for specific detection of Listeria monocytogenes in food samples. The method consists of three steps: i) enrichment of food microflora (16 h), ii) selective isolation of Listeria sp. exploiting immunomagnetic separation (2–3 h) followed by iii) precise identification of Listeria sp. and Listeria monocytogenes using duplex PCR. PCR primers specific to part of 16S rRNA were used in order to identify the members of Listeria genus. The specific identification of Listeria monocytogenes was accomplished exploiting a pair of primers specific to gene encoding invasion-associated protein – iap (4–5 h). Amplification products, 1003 bp and 593 bp respectively, were separated by electrophoresis and visualized by UV detection. The optimized IMS-PCR method was used to test the presence of Listeria sp. and Listeria monocytogenes in food samples (ground meat, low-fat milk and cheese [olomoucké tvarůžky]). A comparison of the efficiency of the bacteria enrichment step by IMS and centrifugation was also performed. The analysis time including enrichment is less than 24 h. The detection limit for Listeria monocytogenes was found between 101–102 cfu per 25 g of food sample.

scholarly journals Species level identification of thermotolerant campylobacters 

2012 ◽  
Vol 50 (No. 12) ◽  
pp. 543-547 ◽  
Author(s):  
I. Kolackova ◽  
R. Karpiskova
Keyword(s):  
Species Identification ◽  
Mixed Cultures ◽  
Species Determination ◽  
The Czech Republic ◽  
Identification Process ◽  
Pcr Method ◽  
Phenotypic Methods ◽  
Level Identification ◽  
Campylobacter Spp

The aim of this study was to compare the phenotypic and genotypic based methods for species identification of thermotolerant campylobacters of human and food origin from the Czech Republic. Phenotypic methods are time-consuming and sometimes lead to intermediate results, therefore replacement by more specific and rapid methods are needed. Out of a total of 911 campylobacter strains tested, 800 human isolates were received from the clinical bacteriology laboratories from 5 regions and 111 foodstuff isolates (raw chicken and pork meat from retail market) originated from the routine examination in our laboratory. Based on the PCR method 85.1% of these strains were identified as C. jejuni, 12.5% as C. coli and 2.3% as mixed cultures of C. jejuni and C. coli. When species determination of campylobacters was based on conventional methods (hippurate hydrolysis test), 28.5% of the isolates were not identified correctly. The mixed cultures of campylobacters have not been detected without further subculturing of strains, which takes several days and enormously extends the identification process. The use of the PCR method showed to be a useful tool for species identification of Campylobacter spp.

Determination of the alleles of HLA I class in Georgian, Kurdish and Czech populations by ARMS-PCR method

1996 ◽  
Vol 47 (1-2) ◽  
pp. 54
Author(s):  
Bendukidze Nina ◽  
Meschishvili Elena ◽  
Ivašková Eva ◽  
Černá Marie ◽  
Churadze Tamaz ◽  
...  
Keyword(s):  
Arms Pcr ◽  
Pcr Method

scholarly journals Determination of Isolated Salmonella Bacteria from Infected Animal Husbandry of Malayer City with using Molecular Methods

2017 ◽  
Vol 7 (1) ◽  
pp. 34
Author(s):  
Alireza Khakzad ◽  
Fatemeh Keshavarzi
Keyword(s):  
Genetic Diversity ◽  
Salmonella Enterica ◽  
Salmonella Enteritidis ◽  
Infected Animal ◽  
Animal Husbandry ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Sequence Elements ◽  
Pcr Method

Salmonella species are gram negative bacteria and members of Enterobacteriaceae family. It has a rod-shaped appearance; it is catalase positive, oxidase negative, non-spore. Salmonella classified into two species, Salmonella Enterica and Salmonella Bangori. Salmonella is now one of the main reasons of diarrhea and vomiting in humans in many countries and especially in industrialized. In a study in Japan 164 Salmonella digestions were collected during 2006 to 2008 which 81 digestions were Salmonella Infantis. Salmonella-specific characteristics are studied in the two phenotype and genotype methods. In this research, with using genotype methods based on PCR, genetic diversity was evaluated; this PCR includes rep-PCR based on repetitive sequence elements (method was done by the use of three primers ERIC, REP and BOX). Studied showed most isolated strains were relevant to Salmonella Enteritidis and dendorogram study showed that the bacteria were grouped in one cluster in dendrogram that all 37 strains were put in a large cluster of Salmonella’s type which is divided into two clusters: Salmonella Enterica and Bongori. The results also in this experiment reflect the efficiency of rep-PCR method by using three ERIC, REP and BOX primers.

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