Isolation, characterization and antioxidant activities of the endophytic fungi from Hosta. Ventricosa

Endophytic fungi, which colonize within a host plant without causing any apparent diseases, have been considered as an important source of bioactive secondary metabolites containing antimicrobial and antioxidant activities. The aim of this research was to isolate the endophytic fungi ofCinnamomum loureiroiand then to screen their antimicrobial and antioxidant activities. A total of 11 fungal endophytes were isolated from healthy leaves ofCinnamomum loureiroibelonging to six genera:Botryosphaeria,Colletotrichum,Diaporthe,Fusarium,Neopestalotiopsis, andPestalotiopsis. All isolated strains were cultured and further extracted with ethyl acetate solvent. Antimicrobial activity of all crude endophytic fungal extracts was analyzed using disc diffusion assay against six bacterial and two fungal pathogens. Crude extracts of strains MFLUCC15-1130 and MFLUCC15-1131 showed broad-spectrum antimicrobial activity against all tested pathogens. Activity againstBacillus cereusandStaphylococcus epidermidiswas notable, showing the lowest minimum inhibitory concentration at 3.91 μg/mL. Antioxidant activity of all crude endophytic fungal extracts was also evaluated based on 2,2-diphenyl-1-picrylhydrazyl assay. Significant antioxidant activity was detected in the crude extracts of fungus MFLUCC15-1130 and MFLUCC15-1131 with IC50of 22.92 ± 0.67 and 37.61 ± 0.49 μg/mL, respectively. Using molecular identification, MFLUCC15-1130 and MFLUCC15-1131 were identified asNeopestalotiopsissp. andDiaporthesp., respectively. The major chemical constituents produced by both crude extracts were identified by gas chromatography-mass spectrometry. Eugenol, myristaldehyde, lauric acid, and caprylic acid were the primary antimicrobial and antioxidant compounds in both crude extracts. This is the first report of eugenol being a biologically active compound ofNeopestalotiopsissp. andDiaporthesp. fungal endophytes. Eugenol has been reported as antimicrobial and antioxidant agents with agronomic applications. Thus the two newly-isolated endophytes may be used for eugenol production, which in turn can be used in a variety of applications.

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Antibacterial and antioxidant activities of the endophytic fungi from medicinal herb Trillium tschonoskii

African Journal of Microbiology Research ◽

10.5897/ajmr11.1115 ◽

2011 ◽

Vol 5 (27) ◽

Author(s):

Yufan Zhou

Keyword(s):

Endophytic Fungi ◽

Antioxidant Activities ◽

Medicinal Herb ◽

Trillium Tschonoskii

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Screening Fungal Endophytes Derived from Under-Explored Egyptian Marine Habitats for Antimicrobial and Antioxidant Properties in Factionalised Textiles

Microorganisms ◽

10.3390/microorganisms8101617 ◽

2020 ◽

Vol 8 (10) ◽

pp. 1617

Author(s):

Ahmed A. Hamed ◽

Sylvia Soldatou ◽

M. Mallique Qader ◽

Subha Arjunan ◽

Kevin Jace Miranda ◽

...

Keyword(s):

Endophytic Fungi ◽

Pathogenic Bacteria ◽

Antioxidant Activities ◽

Human Fibroblasts ◽

Fungal Endophytes ◽

Antioxidant Properties ◽

Chemical Screening ◽

Marine Habitats ◽

Bacteria And Fungi ◽

Promising Source

Marine endophytic fungi from under-explored locations are a promising source for the discovery of new bioactivities. Different endophytic fungi were isolated from plants and marine organisms collected from Wadi El-Natrun saline lakes and the Red Sea near Hurghada, Egypt. The isolated strains were grown on three different media, and their ethyl acetate crude extracts were evaluated for their antimicrobial activity against a panel of pathogenic bacteria and fungi as well as their antioxidant properties. Results showed that most of the 32 fungal isolates initially obtained possessed antimicrobial and antioxidant activities. The most potent antimicrobial extracts were applied to three different cellulose containing fabrics to add new multifunctional properties such as ultraviolet protection and antimicrobial functionality. For textile safety, the toxicity profile of the selected fungal extract was evaluated on human fibroblasts. The 21 strains displaying bioactivity were identified on molecular basis and selected for chemical screening and dereplication, which was carried out by analysis of the MS/MS data using the Global Natural Products Social Molecular Networking (GNPS) platform. The obtained molecular network revealed molecular families of compounds commonly produced by fungal strains, and in combination with manual dereplication, further previously reported metabolites were identified as well as potentially new derivatives.

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Screening and taxonomic identification of endophytic fungi with antitumor and antioxidant activities from Artemisia lactiflora

China Journal of Chinese Materia Medica ◽

10.4268/cjcmm20140315 ◽

2014 ◽

Keyword(s):

Endophytic Fungi ◽

Antioxidant Activities ◽

Taxonomic Identification

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ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES IN ENDOPHYTIC FUNGI ISOLATED FROM WITHANIA SOMNIFERA

INDIAN DRUGS ◽

10.53879/id.53.01.10386 ◽

2016 ◽

Vol 53 (01) ◽

pp. 18-24

Author(s):

K. S. Patil ◽

◽

M. Madki

Keyword(s):

Antioxidant Activity ◽

Tissue Culture ◽

Secondary Metabolites ◽

Endophytic Fungi ◽

Withania Somnifera ◽

Antioxidant Activities ◽

Stress Agent ◽

Antimicrobial And Antioxidant Activity ◽

Bioactive Potential ◽

Culture Technology

Withania somnifera (Solanaceae) is a rich source of the alkaloids and steroidal lactones. It is mainly used as aphrodisiac, an anti-stress agent, anti tumour agent adaptogen and general tonic for body. Plant tissue culture technology is studied for the production of secondary metabolites from Withania somnifera. This study was conducted to characterize and explore the endophytic fungi of Withania somnifera for their bioactive potential. A total of fifteen strains of endophytic fungi were isolated and characterized by morphological and microscopic characters. The fungi were fermented and extracted with ethyl acetate and acetone and screened for antimicrobial and antioxidant activity. Different bioactivities were found to be present in different taxa. The fifteen strains of endophytic fungi were tested for antimicrobial and antioxidant activity out of which six strains showed antimicrobial spectra and seven strains showed antioxidant activity, while remaining strains were inactive.

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Antibacterial and antioxidant activities of endophytic fungi and nettle (Urtica dioica L.) leaves as their host

Cellular and Molecular Biology ◽

10.14715/cmb/2021.67.3.33 ◽

2021 ◽

Vol 67 (3) ◽

pp. 204-211

Author(s):

Djouza Salmi ◽

Catherine Riou ◽

Mohammad Issawi ◽

Yacine Titouche ◽

Veronica Ambrosini ◽

...

Keyword(s):

Endophytic Fungi ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Urtica Dioica ◽

Biologically Active ◽

Bioactive Metabolites ◽

Wide Range ◽

Thiol Content ◽

Pharmaceutical Industries

Nettle (Urtica dioica L), as a plant rich in biologically active compounds, is one of the most important plants used in herbal medicine. Studies have shown that this plant has antioxidant, antiplatelet, hypoglycemic and hypocholesterolemia effects. In this study, we characterized three Alternaria endophytic fungi isolated from their host U. dioica. We hypothesized that these endophytic fungi can produce new bioactive metabolites, which may possess the bioactive property with potential application in the medical and pharmaceutical industries. The antibacterial activity was evaluated against reference and isolated strains, including Methicillin-Resistant Staphylococcus aureus. A wide range of antimicrobial activities similar to those measured in nettle leaves was detected especially for Alternaria sorghi. Furthermore, the highest antioxidant activity detected with DPPH free radical scavenging was measured for A. sorghi and nettle leaves ethyl acetate extracts. In addition, whereas catalase activity was similar in the three isolated fungi and nettle leaves, total thiol content and superoxide dismutase activity were significantly higher in leaves. A. sorghi showed the best activities compared to other isolated fungi. The characterization and further production of bioactive compounds produced by this endophyte should be investigated to fight bacteria and especially those that develop drug multi-resistance.

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Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated fromCostus spiralis(Jacq.) Roscoe (Costaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/190543 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Poliana Guerino Marson Ascêncio ◽

Sérgio Donizeti Ascêncio ◽

Aline Aires Aguiar ◽

Adriana Fiorini ◽

Raphael Sanzio Pimenta

Keyword(s):

Antioxidant Activity ◽

Endophytic Fungi ◽

Antioxidant Activities ◽

Antimicrobial Activities ◽

Total Phenol Content ◽

Strong Positive Correlation ◽

Antioxidant Power ◽

Pearson Coefficient ◽

Ferric Reducing Antioxidant Power ◽

Bioactive Potential

Costus spiralis(Costaceae) is a species native to the Amazon region and is used in traditional medicine. The endophytic fungi used in this study were obtained from leaves of this plant. 13 strains were selected to obtain hydroethanolic extracts and were submitted to hydroalcoholic extraction and evaluated for antioxidant activity by DPPH (2,2-difenil-1-picrilhidrazil) and FRAP (ferric reducing antioxidant power), and all of the fungi had positive results. The antimicrobial action of crude extracts had a good range of activities. All extracts had inhibitory activities against the yeasts ofCandida albicansandC. parapsilosis, with 125 to 500 μg/mL MIC. Eight extracts had antimicrobial activities againstBacillus subtilis(MIC from 62.4 to 125 μg/mL), 5 againstPseudomonas aeruginosa(MIC from 125 to 500μg/mL), 2 againstSalmonella enterica(MIC from 125 to 62.5 μg/mL), and 2 againstEnterococcus faecalis(MIC from 500 to 125 μg/mL). The presence of secondary metabolites, including coumarins, was observed during chemical evaluation by thin layer chromatography. Total phenol content was estimated, and a strong positive correlation to antioxidant activity was observed, according to its Pearson coefficient. This is the first report of the bioactive potential of endophytic fungi isolated from the Costaceae family in Brazilian ecosystems.

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Screening of Biological Effectiveness of Endophytic Fungi Associated with Plectranthus amboinicus: A focus on Aspergillus terreus

International Journal of Pharmaceutical Sciences Review and Research ◽

10.47583/ijpsrr.2021.v69i01.004 ◽

2021 ◽

Vol 69 (1) ◽

Author(s):

Tejaswini H.K ◽

Jayashankar M ◽

Mohammed Ali Saeed

Keyword(s):

Endophytic Fungi ◽

Antioxidant Activities ◽

Fungal Endophytes ◽

Positive Control ◽

Bioactive Molecules ◽

Antidiabetic Activity ◽

Edible Plant ◽

Diverse Range ◽

Biological Effectiveness ◽

Plectranthus Amboinicus

Endophytic fungi produce a diverse range of bioactive molecules, which aids to produce a rich source of different types of medicines. The present study aims to evaluate the antimicrobial, antioxidant, and antidiabetic activity of fungal endophytes associated with edible leaves Plectranthus amboinicus (Indian borage). The results of preliminary screening of antibacterial activity showed inhibition against all tested pathogens similarly, crude ethyl acetate extract of all the isolates in the secondary screening of antidiabetic activity showed a good zone of inhibition with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The antioxidant activities were also determined by using the TPC, FRAP, and DPPH assay, which showed considerable variations concerning their positive control. The correlation among antioxidant assays indicated a strong correlation between TPC and DPPH. Also, the antidiabetic activity by alpha-amylase inhibition assay showed good inhibitory activity in different concentrations. Thus, the study suggested that endophytes from this edible plant could be potential source for the production of broad-spectrum antimicrobial, antioxidant, and antidiabetic compounds.

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