scholarly journals Chemical Assessment and Antimicrobial and Antioxidant Activities of Endophytic Fungi Extracts Isolated fromCostus spiralis(Jacq.) Roscoe (Costaceae)

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Poliana Guerino Marson Ascêncio ◽  
Sérgio Donizeti Ascêncio ◽  
Aline Aires Aguiar ◽  
Adriana Fiorini ◽  
Raphael Sanzio Pimenta
Keyword(s):  
Antioxidant Activity ◽  
Endophytic Fungi ◽  
Antioxidant Activities ◽  
Antimicrobial Activities ◽  
Total Phenol Content ◽  
Strong Positive Correlation ◽  
Antioxidant Power ◽  
Pearson Coefficient ◽  
Ferric Reducing Antioxidant Power ◽  
Bioactive Potential

Costus spiralis(Costaceae) is a species native to the Amazon region and is used in traditional medicine. The endophytic fungi used in this study were obtained from leaves of this plant. 13 strains were selected to obtain hydroethanolic extracts and were submitted to hydroalcoholic extraction and evaluated for antioxidant activity by DPPH (2,2-difenil-1-picrilhidrazil) and FRAP (ferric reducing antioxidant power), and all of the fungi had positive results. The antimicrobial action of crude extracts had a good range of activities. All extracts had inhibitory activities against the yeasts ofCandida albicansandC. parapsilosis, with 125 to 500 μg/mL MIC. Eight extracts had antimicrobial activities againstBacillus subtilis(MIC from 62.4 to 125 μg/mL), 5 againstPseudomonas aeruginosa(MIC from 125 to 500μg/mL), 2 againstSalmonella enterica(MIC from 125 to 62.5 μg/mL), and 2 againstEnterococcus faecalis(MIC from 500 to 125 μg/mL). The presence of secondary metabolites, including coumarins, was observed during chemical evaluation by thin layer chromatography. Total phenol content was estimated, and a strong positive correlation to antioxidant activity was observed, according to its Pearson coefficient. This is the first report of the bioactive potential of endophytic fungi isolated from the Costaceae family in Brazilian ecosystems.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals Comparative Study on Phytochemical Profile and Antioxidant Activity of an Epiphyte, Viscum album L. (White Berry Mistletoe), Derived from Different Host Trees

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1191
Author(s):  
Mahak Majeed ◽  
Tanveer Bilal Pirzadah ◽  
Manzoor Ahmad Mir ◽  
Khalid Rehman Hakeem ◽  
Hesham F. Alharby ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Juglans Regia ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Viscum Album ◽  
Total Phenol Content ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

The study aimed at evaluating the antioxidant profile of a medicinal epiphyte Viscum album L. harvested from three tree species, namely, Populus ciliata L, Ulmus villosa L., and Juglans regia L. The crude extracts were obtained with ethanol, methanol, and water and were evaluated for the total phenol content (TPC), total flavonoid content (TFC), and antioxidant activities using total reducing power (TRP), ferric reducing antioxidant power (FRAP), 1, 1-diphenyl 1-2-picryl-hydrazyl (DPPH), superoxide radical scavenging (SOR), and hydroxyl radical scavenging (•OH) assays. Our results showed that crude leaf extracts of plants harvested from the host Juglans regia L. exhibited higher yields of phytochemical constituents and noticeable antioxidative properties. The ethanolic leaf samples reported the highest phenols (13.46 ± 0.87 mg/g), flavonoids (2.38 ± 0.04 mg/g), FRAP (500.63 ± 12.58 μM Fe II/g DW), and DPPH (87.26% ± 0.30 mg/mL). Moreover, the highest values for TRP (4.24 ± 0.26 μg/mL), SOR (89.79% ± 0.73 mg/mL), and OH (67.16% ± 1.15 mg/mL) were obtained from aqueous leaf extracts. Further, Pearson correlation was used for quantifying the relationship between TPC, TFC, and antioxidant (FRAP, DPPH, SOR, OH) activities in Viscum album L. compared to their hosts. It was revealed that the epiphyte showed variation with the type of host plant and extracting solvent.

scholarly journals Antioxidant Activities and Volatile Flavor Components of Selected Single-Origin and Blend Chocolates

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3648
Author(s):  
Lucia Godočiková ◽  
Eva Ivanišová ◽  
Grzegorz Zaguła ◽  
Luis Noguera-Artiaga ◽  
Ángel A. Carbonell-Barrachina ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Content ◽  
Composition Analysis ◽  
High Positive Correlation ◽  
Antioxidant Power ◽  
Single Origin ◽  
Ferric Reducing Antioxidant Power

The biological activity of chocolates gains more and more attention of consumers. Its antioxidant properties depend, among other factors, mainly on the origin of cocoa and the characteristics that this origin gives to the final product. Therefore, the aim of the study was to measure and compare the total content of polyphenols, antioxidant activity, and key odorants of commercial chocolates made from blend cocoa with single-origin ones. The highest content of polyphenols was found in 90% blend cocoa chocolate and single-origin samples, while the lowest content was exhibited by 100% chocolate from blend cocoa mass. The highest antioxidant activity measured by 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) assays was observed in the sample of chocolate with 90% cocoa solids from blend mass, followed by single-origin chocolates. A high positive correlation between ABTS assay and the total polyphenol and phenolic acids’ content, as well as among the total content of polyphenols, flavonoids, and phenolic acids was found. Mineral composition analysis showed that dark chocolate is a valuable source of some elements, especially Mg, Fe, and Zn. Potentially toxic elements were not detected or below permitted limits. Moreover, it was noticed that the main volatile compound in all tested samples was acetic acid, but pyrazines were considered the most important group of chocolate odorants.

scholarly journals Zn(II) Complex of Plant Phenolic Chlorogenic Acid: Antioxidant, Antimicrobial and Structural Studies

Materials ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 3745
Author(s):  
Monika Kalinowska ◽  
Justyna Sienkiewicz-Gromiuk ◽  
Grzegorz Świderski ◽  
Anna Pietryczuk ◽  
Adam Cudowski ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chlorogenic Acid ◽  
Density Functional ◽  
Salmonella Enteritidis ◽  
Antioxidant Properties ◽  
Antimicrobial Activities ◽  
Butylated Hydroxytoluene ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Thermochemical Parameters

The structure of the Zn(II) complex of 5-caffeoylquinic acid (chlorogenic acid, 5-CQA) and the type of interaction between the Zn(II) cation and the ligand were studied by means of various experimental and theoretical methods, i.e., electronic absorption spectroscopy UV/Vis, infrared spectroscopy FT-IR, elemental, thermogravimetric and density functional theory (DFT) calculations at B3LYP/6-31G(d) level. DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), FRAP (ferric reducing antioxidant power), CUPRAC (cupric reducing antioxidant power) and trolox oxidation assays were applied in study of the anti-/pro-oxidant properties of Zn(II) 5-CQA and 5-CQA. The antimicrobial activity of these compounds against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Salmonella enteritidis and Candida albicans was tested. An effect of Zn(II) chelation by chlorogenic acid on the anti-/pro-oxidant and antimicrobial activities of the ligand was discussed. Moreover, the mechanism of the antioxidant properties of Zn(II) 5-CQA and 5-CQA were studied on the basis of the theoretical energy descriptors and thermochemical parameters. Zn(II) chlorogenate showed better antioxidant activity than chlorogenic acid and commonly applied natural (L-ascorbic acid) and synthetic antioxidants (butylated hydroxyanisol (BHA) and butylated hydroxytoluene (BHT)). The pro-oxidant activity of Zn(II) 5-CQA was higher than the ligand and increased with the rise of the compound concentration The type of Zn(II) coordination by the chlorogenate ligand strongly affected the antioxidant activity of the complex.

scholarly journals Antibacterial and Antioxidant Activities of Derriobtusone A Isolated fromLonchocarpus obtusus

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Mayron Alves Vasconcelos ◽  
Francisco Vassiliepe Sousa Arruda ◽  
Daniel Barroso de Alencar ◽  
Silvana Saker-Sampaio ◽  
Maria Rose Jane Ribeiro Albuquerque ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Test Results ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
E Coli ◽  
Planktonic Growth ◽  
Ferric Reducing Antioxidant Power

This study evaluated the effect of derriobtusone A, a flavonoid isolated fromLonchocarpus obtusus, on two important pathogenic bacteria,Staphylococcus aureusandEscherichia coli, as well as its antioxidant activity and toxicity. Planktonic growth assays were performed, and the inhibition of biofilm formation was evaluated. In addition, antioxidant activity was assessed by DPPH radical scavenging assay, ferrous ion chelating assay, ferric-reducing antioxidant power assay, andβ-carotene bleaching assay. Toxicity was evaluated by the brine shrimp lethality test. Results showed that derriobtusone A completely inhibited the planktonic growth ofS. aureusat 250 and 500 μg/mL; however, it did not have the same activity onE. coli. Derriobtusone A reduced the biomass and colony-forming unit (cfu) ofS. aureusbiofilm at concentrations of 250 and 500 μg/mL. In various concentrations, it reduced the biofilm biomass ofE. coli, and, in all concentrations, it weakly reduced the cfu. Derriobtusone A showed highly efficient antioxidant ability in scavenging DPPH radical and inhibitingβ-carotene oxidation. The compound showed no lethality toArtemiasp. nauplii. In conclusion, derriobtusone A may be an effective molecule againstS. aureusand its biofilm, as well as a potential antioxidant compound with no toxicity.

scholarly journals PENGARUH WAKTU FERMENTASI TERHADAP AKTIVITAS ANTIOKSIDAN DAN KADAR BETASIANIN MINUMAN FUNGSIONAL DARI BUAH NAGA (Hylocereus polyrhizus) DAN UMBI BIT (Beta vulgaris)

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Yati Maryati ◽  
Agustine Susilowati ◽  
Nina Artanti ◽  
Puspa Dewi Narij Lotulung ◽  
Aspiyanto Aspiyanto
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Iron Ions ◽  
Antioxidant Power ◽  
Fermentation Time ◽  
Reduction Of Iron ◽  
Dragon Fruit ◽  
Functional Beverages ◽  
Ferric Reducing Antioxidant Power

Effect of Fermentation on Antioxidant Activities and Betacyanin Content of Functional Beverages from Dragon Fruit and BeetrootKombucha is a traditional beverage prepared by fermenting polyphenol-rich tea using a consortium of bacteria and yeast (SCOBY). Dragon fruit and beetroot contain a significantly high amount of polyphenols and betacyanin as antioxidants which are beneficial in reducing the risk of cardiovascular disease, cancer, and the body's natural degeneration related to the early-aging process. This study aims to determine changes in antioxidant activity and levels of betacyanin from fermented dragon fruit and beetroot as functional drinks during different fermentation times. The results showed that there was a correlation between fermentation time and antioxidant activity in its ability to counteract free radicals, ferric reducing antioxidant power (FRAP), and the levels of betacyanin produced. Longer fermentation time caused an increase in free radical inhibition and reduction of iron ions but reduced the levels of betacyanin. The optimum value of free radical inhibitory activity (DPPH) was obtained in the 12-day fermented dragon fruit drink with an inhibitory value of 80.76%, ability to reduce iron ions by 197.94 µg ascorbic acid mL-1, and betacyanin level of 0.055 mg L-1Keywords: antioxidants, dragon fruit, betacyanin, beetroot, functional beverages ABSTRAKKombucha merupakan minuman tradisional hasil olahan fermentasi teh yang kaya polifenol dengan konsorsium bakteri dan yeast (SCOBY). Buah naga dan umbi bit memiliki polifenol dan senyawa betasianin yang cukup tinggi sebagai antioksidan yang bermanfaat dalam menurunkan risiko penyakit kardiovaskular, penyakit kanker dan degenerasi alami tubuh terkait proses penuaan dini. Penelitian ini bertujuan untuk mengetahui perubahan aktivitas antioksidan dan kadar betasianin dari fermentasi buah naga maupun umbi bit sebagai minuman fungsional selama waktu fermentasi yang berbeda. Hasil penelitian menunjukkan bahwa adanya korelasi antara waktu fermentasi terhadap aktivitas antioksidan dalam kemampuannya menangkal radikal bebas, kemampuan mereduksi ion besi (FRAP), dan kadar betasianin yang dihasilkan. Semakin lama waktu fermentasi menyebabkan peningkatan penghambatan radikal bebas dan reduksi ion besi, namun menurunkan kadar betasianin. Nilai aktivitas penghambatan radikal bebas (DPPH) optimum diperoleh dari minuman fermentasi buah naga selama waktu fermentasi 12 hari dengan nilai penghambatan sebesar 80,76%, kemampuannya dalam mereduksi ion besi sebesar 197,94 µg asam askorbat mL–1, dan kadar betasianin sebesar 0,055 mg L–1

scholarly journals Antioxidant activity and polyphenol composition of Pistacia terebinthus fruit from Tessala (Western Algeria)

2021 ◽  
Vol 5 (11) ◽  
pp. 30-34
Author(s):  
Nazim Bellifa ◽  
Abdelhak Ismail Benhaddou ◽  
Houssem Eddine Ferkous ◽  
Mohammed Adil Selka ◽  
Houari Toumi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Scavenging Effect ◽  
Coffee Substitute ◽  
Ferric Reducing Antioxidant Power ◽  
Pistacia Terebinthus

Background: Consumption of traditional herbal beverages has been generally increased in the last decades, Terebinth coffee, known as ‘‘menengic coffee’’ in Turkish, is one of the most consumed herbal coffees in Turkey, turpentine tree is one of the components of the Mediterranean bush, particularly in Algeria, known as Betoum el Kiffan is largely used as food and in traditional medicine. Aims: In this study, Total phenol, flavonoid content, and antioxidant activity of three extracts of Pistacia trebinthus fruit growing in Algeria was measured using radical scavenging activity tests and metal-related tests including, ferric-reducing antioxidant power (FRAP). The chemical composition profile of the fruits and the coffee brands was identified by thin-layer chromatography, the effects of roasting method of this fruit was rivaled also. Materials and Methods: The total phenolic content of the extracts was determined using the Folin-Ciocalteu method. All extracts of the terebinth fruits and coffee brands displayed a high DPPH scavenging effect. Results: The results of the ferric-reducing antioxidant power show that the reduction capacity is proportional to the increase in the concentration of the samples. All the extracts of the plant exhibit antioxidant activities lower than those of the reference product besides the infusion extract of the P. terebinthus roasted coffee, which is the most active with an optical density of 1.68 nm at a concentration of 400 μg/mL. The chromatography results show that the various extracts of Pistacia terebinthus fruit carry a large number of polyphenols, in particular the carboxylic acids phenols. Conclusions: The plant can be considered as a coffee substitute and opens up promising avenues for the food and pharmaceutical industry in Algeria. Keywords: Antioxidant, Pistacia terebinthus, Coffee, FRAP, polyphenol.

ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES IN ENDOPHYTIC FUNGI ISOLATED FROM WITHANIA SOMNIFERA

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (01) ◽  
pp. 18-24
Author(s):  
K. S. Patil ◽  
◽  
M. Madki
Keyword(s):  
Antioxidant Activity ◽  
Tissue Culture ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Withania Somnifera ◽  
Antioxidant Activities ◽  
Stress Agent ◽  
Antimicrobial And Antioxidant Activity ◽  
Bioactive Potential ◽  
Culture Technology

Withania somnifera (Solanaceae) is a rich source of the alkaloids and steroidal lactones. It is mainly used as aphrodisiac, an anti-stress agent, anti tumour agent adaptogen and general tonic for body. Plant tissue culture technology is studied for the production of secondary metabolites from Withania somnifera. This study was conducted to characterize and explore the endophytic fungi of Withania somnifera for their bioactive potential. A total of fifteen strains of endophytic fungi were isolated and characterized by morphological and microscopic characters. The fungi were fermented and extracted with ethyl acetate and acetone and screened for antimicrobial and antioxidant activity. Different bioactivities were found to be present in different taxa. The fifteen strains of endophytic fungi were tested for antimicrobial and antioxidant activity out of which six strains showed antimicrobial spectra and seven strains showed antioxidant activity, while remaining strains were inactive.

scholarly journals Antioxidant activities of Dichrostachys cinerea (L.) Wight et Arn (Leguminosae): Correlations between the Polyphenol Level and the Antioxidant Activity

2020 ◽  
pp. 1-11
Author(s):  
Reine Raïssa Rolande Aworet Samseny ◽  
Line-Edwige Mengome ◽  
Sophie Aboughe Angone
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Antiradical Activity ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Dpph Radical ◽  
Dichrostachys Cinerea ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Aims: Free radicals are involved in many diseases. Antioxidants help control them. The overproduction of free radicals or reduction of natural antioxidants promotes the destruction of cells in our body, for example in neurodegenerative diseases. The aim of our study is to evaluate the correlation between the level of polyphenol and antioxidant activity of Dichrostachys cinerea barks he work was carried out at the phytochemistry department of the Institute of Pharmacopoeia and Traditional Medicine (Gabon). Methodology: Different extracts of Dichrostachys cinerea barks were obtained with polarity solvent (dichloromethane, ethyl acetate, butanol, methanol and water).  The antioxidant activity is evaluating using respectively FRAP method (Ferric Reducing Antioxidant Power), antiradical activity by the method of inhibition of DPPH radical (2.2-diphenyl-1-picrylhydrazyl), cations ABTS radical (2.2'-azinobis-[3-acid-6-sulfonic ethylenzothiazoline]). Results: Ethyl acetate and dichloromethane extract have the higher value for polyphenolic compounds, respectively 52,27±0,66 mg EAG / g and 49,72±0,55 mg EAG / g. Aqueous extract have the lower value 20,67±1,05 mg EAG / g. The different fractions of Dichrostachys cinerea have antioxidant effect, and this effect is in correlation with its constituents: polyphenols, tannins and flavonoids. Results were compared to standard antioxidants such as ascorbic acid and quercetin. Conclusion: This study confirms higher is the level of polyphenols greater is the antioxidant power.

scholarly journals Anti-hyperglycaemic and anti-oxidant activities of five Nigerian antidiabetic plants

2019 ◽  
Vol 37 (2) ◽  
pp. 71-84
Author(s):  
M. D. Ayoola ◽  
A. C. Adebajo ◽  
E. M. Obuotor ◽  
T. O. Oladapo ◽  
T. C. Fleischer
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Diabetic Rats ◽  
Antioxidant Power ◽  
Glucose Levels ◽  
Methanolic Extracts ◽  
Normal Glucose ◽  
Ferric Reducing Antioxidant Power

Leaves of Olax subscorpioidea, Hoslundia opposita, Cleistopholis patens, Plumbago zeylanica and Dioscoreophyllum cumminsii that are used as anti-diabetics were evaluated for hyperglycaemic-lowering and antioxidant activities to justify their Nigerian ethnomedicinal usage. Leaf methanolic extracts (100, 200 and 400 mg/kg, p.o.) were assayed in normal, glucose- and alloxan-induced diabetic rats, while 1,1,-diphenyl-2-picrylhydrazyl, total antioxidant capacity, ferric reducing antioxidant power and hydroxyl radical scavenging tests were used for their antioxidant activity. Effects of n-hexane, dichloromethane, ethylacetate and aqueous partition fractions of the three most active anti-hyperglycaemic extracts were also tested in glucose-loaded rats. In normoglycaemic rats, all extracts generally lacked activity, while with glucose-loaded rats, only O. subscorpioidea (200mg/kg) and C. patens (400 mg/kg) at 1 h and O. subscorpioidea (200, 400 mg/kg) and C. patens (400 mg/kg) at 2-4 hours gave lowered (p < 0.05) blood glucose levels than glibenclamide (5 mg/kg), while activity of H. opposita and P. zeylanica (200 mg/kg) were only comparable to glibenclamide. When daily fed for fourteen days to alloxan-induced diabetic rats, all extracts, at their most active doses, gave significantly higher activity than glibenclamide. Olax subscorpioidea leaf extract had the highest hyperglycaemic-lowering and least antioxidant activities. Highest antioxidant activity of H. opposita may suggest some contribution of antioxidant property to its hyperglycaemic-lowering activity. The glucose-lowering and insulinotropic constituents of O. subscorpioidea, H. opposita and C. patens should be concentrated in their aqueous, aqueous and dichloromethane fractions, respectively. Anti-hyperglycaemic ethnomedicinal use of these plants was justified and H. opposita has additional antioxidant property. Keywords: Antioxidant activity; Diabetes mellitus; Nigerian anti-diabetic plants 

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