Enhancing Phenolic Contents and Antioxidant Potentials ofAntidesma thwaitesianumby Supercritical Carbon Dioxide Extraction

Supercritical fluid extraction (SFE) has increasingly gained attention as an alternative technique for extraction of natural products without leaving toxic residues in extracts.Antidesma thwaitesianumMuell. Arg. (Phyllanthaceae), or ma mao, has been reported to exhibit antioxidant health benefits due to its phenolic constituents. To determine whether SFE technique could impact on phenolic contents and associated antioxidant potentials, ripe fruits ofAntidesma thwaitesianum(Phyllanthaceae) were extracted using supercritical carbon dioxide (SC-CO2) and conventional solvents (ethanol, water). The results showed that the SC-CO2extract contained significantly higher yield, total phenolic, flavonoid, and proanthocyanidin contents than those obtained from ethanol and water. It also demonstrated the greatest antioxidant activities as assessed by ABTS radical cation decolorization, DPPH radical scavenging, and ferric reducing antioxidant power (FRAP) assays. Further analysis using high-performance liquid chromatography with diode array and mass spectrometry detectors (HPLC-DAD/MSD) revealed the presence of catechin as a major phenolic compound ofAntidesma thwaitesianum(Phyllanthaceae), with the maximum amount detected in the SC-CO2extract. These data indicate that SFE technology improves both quantity and quality ofAntidesma thwaitesianumfruit extract. The findings added more reliability of using this technique to produce high added value products from this medicinal plant.

Download Full-text

Supercritical Carbon Dioxide (scCO2) Extraction of Phenolic Compounds from Lavender (Lavandula angustifolia) Flowers: A Box-Behnken Experimental Optimization

Molecules ◽

10.3390/molecules24183354 ◽

2019 ◽

Vol 24 (18) ◽

pp. 3354 ◽

Cited By ~ 5

Author(s):

Katarzyna Tyśkiewicz ◽

Marcin Konkol ◽

Edward Rój

Keyword(s):

Carbon Dioxide ◽

Phenolic Compounds ◽

Supercritical Carbon Dioxide ◽

Extraction Method ◽

Positive Influence ◽

Total Phenolic ◽

Statistical Experimental Design ◽

Lavandula Angustifolia ◽

Phenolic Contents ◽

Supercritical Carbon

Due to their numerous health benefits associated with various diseases and anti-oxidation properties, the phenolic compounds collectively referred to as phytochemicals have attracted a lot of interest, however, a single extraction method for polyphenols has not been developed yet. Supercritical fluid extraction, a green extraction method, provides the final product without organic solvent residues. In this work the extraction of lavender was performed using supercritical carbon dioxide. A statistical experimental design based on the Box-Behnken (B-B) method was planned, and the extraction yields and total phenolic contents were measured for three different variables: pressure, temperature and extraction time. The ranges were 200–300 bar, 40–60 °C and 15–45 min. The extracts yields from scCO2 extraction were in the range of 4.3–9.2 wt.%. The highest yield (9.2 wt.%) was achieved at a temperature of 60 °C under the pressure of 250 bar after 45 min. It also corresponded to the highest total phenolic content (10.17 mg GAE/g extract). Based on the study, the statistically generated optimal extraction conditions to obtain the highest total phenolic compounds concentration from flowers of Lavandula angustifolia were a temperature of 54.5 °C, pressure of 297.9 bar, and the time of 45 min. Based on the scavenging activity percentage (AA%) of scCO2 extracts, it is concluded that the increase of extraction pressure had a positive influence on the increase of AA% values.

Download Full-text

Phenotypic diversity of pea (Pisum sativum L.) varieties and the polyphenols, flavonoids, and antioxidant activity of their seeds

Ciência Rural ◽

10.1590/0103-8478cr20190196 ◽

2020 ◽

Vol 50 (5) ◽

Author(s):

Tianyao Zhao ◽

Wenjin Su ◽

Yang Qin ◽

Liyun Wang ◽

Yufan Kang

Keyword(s):

Pisum Sativum ◽

Principal Components ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Morphological Characteristics ◽

Total Phenolic ◽

Antioxidant Power ◽

Phenolic Contents ◽

Pisum Sativum L ◽

Wide Range

ABSTRACT: Pea (Pisum sativum L.) is the fourth leading legume crop in the world, and its demand is increasing. In this study, the morphological characteristics (seed shape, seed surface, seed coat color, hilum color, cotyledon color, 100-seed weight and color values), total phenolic content (TPC), total flavonoid content (TFC), 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radical scavenging capacity and ferric reducing antioxidant power (FRAP) of 75 pea cultivars were investigated. Results showed rich genetic diversity and a wide range of phenolic contents and antioxidant activities. Sixteen varieties enriched with phenolic contents and high antioxidant activities were screened out. A significant correlation was reported among color values, TPC, TFC, ABTS and FRAP. Principal component analysis (PCA) extracted four principal components with a total cumulative contribution of 81.29%. Hierarchical cluster analysis based on the four extracted principal components resulted in a dendrogram dividing the peas into three groups. In addition, dark pea seeds have potential as a functional food in addition to their traditional role in providing dietary protein and fibre. This study provided a scientific basis for the breeding of pea varieties, development of new products and improvement of pea resource utilization.

Download Full-text

Antioxidant Activity and Total Phenolic Content of 24 Lamiaceae Species Growing in Iran

Natural Product Communications ◽

10.1177/1934578x1000500219 ◽

2010 ◽

Vol 5 (2) ◽

pp. 1934578X1000500 ◽

Cited By ~ 4

Author(s):

Omidreza Firuzi ◽

Katayoun Javidnia ◽

Maryam Gholami ◽

Mohammad Soltani ◽

Ramin Miri

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging ◽

Dry Weight ◽

Natural Antioxidants ◽

Total Phenolic ◽

Dpph Assay ◽

Antioxidant Power ◽

Phenolic Contents ◽

Methanolic Extracts ◽

Ferric Reducing Antioxidant Power

The antioxidant activities of the methanolic extracts of 9 Salvia species and 15 other Lamiaceae plants growing in Iran were evaluated using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values ranged form 8.5 to 79.0 μM quercetin equivalents/g dry weight, and IC50 values in the DPPH assay from 115.7 to 1350.2 μg dry weight/mL. Salvia species showed the highest antioxidant activities. S. santolinifolia, S. eremophila and S. palestina, which have not been studied before, were the most active plants. These were more active than the previously studied species from this family, such as S. multicaulis and Marrubium vulgare. S. hydrangea and Gontscharovia popovii also showed high antioxidant activities. FRAP and DPPH assay results showed good correlations with the total phenolic contents of the plants, measured by the Folin-Ciocalteau assay (r2 = 0.925 and 0.799, respectively, p < 0.0001). In conclusion, our study shows that some Lamiaceae plants growing in Iran represent good potential sources of natural antioxidants useful for either prevention or treatment of oxidative stress-related diseases.

Download Full-text

Antioxidant Activities and Phenolic Composition of Apple Peel, Core and Flesh Extracts on Selected Apple Cultivars

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.554-556.1103 ◽

2012 ◽

Vol 554-556 ◽

pp. 1103-1109 ◽

Cited By ~ 1

Author(s):

Gui Zhi Zhang ◽

Bao Ping Ji ◽

Gang Chen

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Total Phenolic ◽

Phenolic Antioxidants ◽

Antioxidant Power ◽

Phenolic Contents ◽

Apple Cultivars ◽

Apple Peels

Phenolic antioxidants have multiple benefits to human health. Polyphenols are responsible for the antioxidant activity in apples. Antioxidant activities were assessed using the ferric reducing/antioxidant power (FRAP) and 2,2-dipheny l-1-picrylhydrazyl (DPPH) radical scavenging assays in different cultivars and different parts of apples grown in China. The phenolic compounds and antioxidant activities differed significantly among the four apple cultivars. Guoguang had the highest phenolic concentration and antioxidant activity and Golden Delicious had the lowest. The peels had the highest total phenolic and flavonoid contents followed by the cores and flesh. Anthocyanins were detected only in red apple peels. Peel and core had greater antioxidant activities than apple flesh. FRAP values were inversely correlated with phenolic contents, whereas no clear relationship could be observed between DPPH values and phenolic contents. The higher levels of phenolic compounds and antioxidant activities of apple peels and cores than flesh may be of technological interest as a valuable source of antioxidants.

Download Full-text

Phenolic compounds and antioxidant activities of grape canes extracts from vineyards

Spanish Journal of Agricultural Research ◽

10.5424/sjar/2016143-8951 ◽

2016 ◽

Vol 14 (3) ◽

pp. e0805 ◽

Cited By ~ 5

Author(s):

Yanlun Ju ◽

Ang Zhang ◽

Yulin Fang ◽

Min Liu ◽

Xianfang Zhao ◽

...

Keyword(s):

Antioxidant Activities ◽

Low Cost ◽

Radical Scavenging ◽

Total Phenolic ◽

Antioxidant Power ◽

Phenolic Contents ◽

Methanolic Extracts ◽

Phenolic Components ◽

Dpph Radical Scavenging ◽

Gallic Acid Equivalent

Grape canes are the main agro-wastes from vineyards. This work studied the antioxidant activities of the defatted methanolic extracts (ME) of canes from 11 genotypes: 5 Vitis vinifera widely known cultivars and 6 Chinese wild varieties from three species (V. amurensis, V. davidii, and V. pentagona) and the antioxidant activities of the ME’s chloroform fractions (CF), ethyl acetate fractions (EAF) and water fractions (WF). Among ME and its three fractions, EAF’s total phenolic contents (TPC) and total flavonoid contents (TFC) were the highest, at 586 mg/g of gallic acid equivalent and 320 mg/g of quercetin equivalent, respectively. The antioxidant power of the fractions/extracts was in the order EAF > ME > WF > CF, based on the DPPH radical-scavenging power and ferric-reducing antioxidant activity, while the order was EAF > CF > WF >ME based on the β-carotene-linoleic acid bleaching activity. Methanolic extracts demonstrated the strongest Fe2+-chelating activity. The antioxidant activities of the extracts/fractions generally correlated with the TPC and TFC in all assays, except with the Fe2+-chelating test. Grape canes from V. davidii had the highest TPC, TFC and antioxidant activities compared with those from other grape species. Catechin, epicatechin and trans-resveratrol were the predominant phenolic components of fractions/extracts. In light of these valuable bioactivities, grape canes from annual pruning practice considered as waste material have good commercial potential for utilization as a promising natural antioxidant in the food, pharmaceutical and cosmetic industries, given its low cost and availability in large amounts.

Download Full-text

Antioxidant and Phenolic Characterization with HPLC of Various Extract of Verbascum glomeratum Linneus

Journal of the chemical society of pakistan ◽

10.52568/000639 ◽

2020 ◽

Vol 42 (2) ◽

pp. 222-222

Author(s):

i dem Aydin i dem Aydin ◽

Akgul Rakhimzhanova Akgul Rakhimzhanova ◽

zge Kilin arslan and Ramazan Mammadov zge Kilin arslan and Ramazan Mammadov

Keyword(s):

Antioxidant Activity ◽

Linoleic Acid ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Antioxidant Power ◽

Phenolic Contents ◽

Phenolic Constituents ◽

Ethanol Extracts ◽

Β Carotene

Verbascum L. species have been used since ancient times in traditional medicine thanks to their bioactive compounds. In this study, we evaluated the antioxidant activity and phenolic contents of ethanolic and acetonic extract of Verbascum glomeratum that collected from Denizli, Turkey. The antioxidant activity were evaluated by using various methods (β-carotene/linoleic acid, ferric reducing power assays (FRAP), radical scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2and#39;-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS), phoshomolibdenum methods) and total phenolic, flavonoid and tannin contents in the extracts were determined. Antioxidant activity was determined as β-carotene/linoleic acid (%58.5and#177;0.021), DPPH (IC50: 1.220and#177;0.025) and ABTS (IC50:1.552and#177;0.021), ferric reducing antioxidant power (FRAP) (4.151and#177;0.056 mgTE/g), phoshomolibdenum assay (8.218and#177;0.015 mgAE/g) total phenolic, total flavonoid and tannin contents was found as (4.41and#177;0.007 mgGAE/mL), (87.142and#177;0.009 and#181;QE/g), and (30.178and#177;0.008 mgCEs/g) respectively, and then 14 different phenolic compounds in ethanol extracts was carried out by HPLC. Epicatechin (2742.09 and#181;g/g) and 2,5dihidroksi (2544.96 and#181;g/g) were the most abundant phenolic constituents in the extracts. According the results, the extract of V. glomeratum may be considered as a potential source of biological agents.

Download Full-text

Determination of Phenolic Contents by HPLC, and Antioxidant, Antimicrobial, Antityrosinase, and Anticholinesterase Activities of Psephellus huber-morathii

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2019/v26i130125 ◽

2019 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Nuriye Korkmaz ◽

Sıla Ozlem Sener ◽

Nuray Balturk ◽

Seyda Kanbolat ◽

Merve Badem ◽

...

Keyword(s):

Antioxidant Capacity ◽

Yersinia Pseudotuberculosis ◽

Methanolic Extract ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Total Phenolic ◽

Antioxidant Power ◽

Phenolic Constituents

The goal of our study was to examine of antioxidant, antimicrobial, anticholinesterase activities, and phenolic composition of Psephellus huber-morathii. The antioxidant activities of extracts have been assessed by Ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging. Phenolic constituents were measured using reverse phase-high performance liquid chromatography (RP-HPLC), and antimicrobial activity was investigated using the agar well diffusion method. Total phenolic content, FRAP, and CUPRAC results of aqueous extract have been better than methanolic extract, except for DPPH activity. Benzoic acid, and p-coumaric acid as major phenolic compounds were specified. Methanolic extract was especially effective against all microorganisms tested except for Yersinia pseudotuberculosis. The methanolic extract have been displayed inhibitory effect on tyrosinase. All extracts have been exhibited lower acetylcholinesterase, and butyrylcholinesterase inhibitory activities than galantamine. P. huber-morathii can be considered in the food, and drug industries due to antioxidant capacity and antimicrobial activities of the species. It can be potential source as anti-browning agents because of its average tyrosinase inhibitory activity.

Download Full-text

Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity

Archives of Biological Sciences ◽

10.2298/abs141204042s ◽

2015 ◽

Vol 67 (3) ◽

pp. 829-840 ◽

Cited By ~ 11

Author(s):

Nemanja Stanisavljevic ◽

Marija Ilic ◽

Zivko Jovanovic ◽

Tihomir Cupic ◽

Dragana Dabic ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Total Phenolic ◽

Phenolic Composition ◽

Phenolic Contents ◽

Metal Chelating ◽

Phenolic Constituents ◽

Seed Coats

The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu?s method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.

Download Full-text

Anti-Inflammatory and Antioxidant Activities ofSalvia fruticosa: An HPLC Determination of Phenolic Contents

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/7178105 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 14

Author(s):

Rima Boukhary ◽

Karim Raafat ◽

Asser I. Ghoneim ◽

Maha Aboul-Ela ◽

Abdalla El-Lakany

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Inflammatory Activity ◽

Total Phenolic ◽

Phenolic Contents ◽

Aerial Parts ◽

Phenolic Constituents ◽

Anti Inflammatory

Objectives.Salvia fruticosaMill. (S. fruticosa) is widely used in folk medicine. Accordingly, the present study was designed to evaluate the antioxidant and anti-inflammatory activities ofS. fruticosa, and to determine the phenolic constituents of its extracts.Methods. The antioxidant activity was determined using 2,2-diphenylpicrylhydrazyl assay. Total phenolic contents were estimated using Folin-Ciocalteu reagent, and high-performance liquid chromatography was performed to identify phenolic constituents. To evaluate the anti-inflammatory activity, carrageenan-induced mouse paw edema was determined plethysmographically.Key Findings. Different plant extracts demonstrated strong radical scavenging activity, where the ethyl acetate extract had the highest value in the roots and the lowest in the aerial parts. This antioxidant activity was correlated to the total phenolic content of different extracts, where rutin and luteolin were the most abundant constituents. Interestingly, both the roots and aerial parts revealed a significant anti-inflammatory activity comparable to diclofenac.Conclusions. This study is the first to demonstrate pharmacologic evidence of the potential anti-inflammatory activity ofS. fruticosa. This activity may partly be due to the radical scavenging effects of its polyphenolic contents. These findings warrant the popular use of the East Mediterranean sage and highlight the potential of its active constituents in the development of new anti-inflammatory drugs.

Download Full-text