Effect of Oral Applied Lead Acetate on the Expression of Caspase-3 on Antral Granulosa Cells and Histopathology of Ovary in Female Wistar Rat (Rattus Norvegicus) Ovaries

2021 ◽  
pp. 6007-6011
Author(s):  
Endyka Erye Frety ◽  
Setyowati Soehato ◽  
Hidayat Sujuti ◽  
Erni Rosita Dewi
Keyword(s):  
Granulosa Cells ◽  
Rattus Norvegicus ◽  
Lead Acetate ◽  
Caspase 3 ◽  
Wistar Rat ◽  
Reproductive Organ ◽  
Oral Exposure ◽  
Female Wistar Rats ◽  
Follicle Atresia ◽  
Group 2

Background: Lead exposure affects several human organs, including the reproductive organ. Aims: This research aims to prove the effect of oral applied lead acetate on the expression of caspase-3 in antral granulosa cells, the diameter of the tertiary follicle, and the amount of follicle atresia inside ovaries. Methods: Twenty-four female Wistar rats (Rattus norvegicus) are classified into 4 groups. Group 1 consists of 6 rats acting as control groups. Group 2, 3, and 4 each consist of 6 rats receiving daily oral lead acetate of 30 ppm, 100 ppm, and 300 ppm in dose, respectively. The experiment will be conducted in 30 days. The rats are then dissected, and the weight of ovaries are measured. The expression of caspase-3 is assessed using immunohistochemistry, while the diameter of tertiary follicles and the amount of follicle atresia are both observed using Hematoxylin-Eosin stain. Results: Oral administration of lead acetate significantly decreased the weight of ovaries. Oral exposure of lead enhances the expression of caspase-3 in antral granulosa cells of all experiment groups, especially in the 300 ppm group. It significantly shrinks tertiary follicles' diameter in rats' ovaries to 100 ppm and 300 ppm groups. It also increases the amount of follicle atresia in the 300 ppm group. Conclusion: Oral exposure of lead enhances the expression of caspase 3 in antral granulosa cells at 300 ppm, shrinks the diameter of tertiary follicles at 100 ppm and 300 ppm doses, and increases the amount of follicle atresia at 300 ppm dose.

scholarly journals The Effectiveness of Chitosan- Pinus merkusii Extract Nanoparticle as Antioxidant and Anti-caspase 3 in against Lead Acetate Toxicity on the Lung of Wistar Rat

2019 ◽  
Vol 53 (2s) ◽  
pp. s135-s142 ◽  
Author(s):  
Sri Agus Sudjarwo ◽  
Giftania Wardani ◽  
Koerniasari Eraiko ◽  
Koerniasari K ◽  
Ernawati E
Keyword(s):  
Lead Acetate ◽  
Caspase 3 ◽  
Wistar Rat ◽  
Pinus Merkusii

scholarly journals The Effect of Propofol on Onset and Sedation in Diabetic Mellitus Rat (Rattus norvegicus)

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Erwin Erwin ◽  
Rusli Rusli ◽  
Zuraidawati Zuraidawati ◽  
Fadillah Irwansyah
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Diabetic Mellitus ◽  
Wistar Strain ◽  
Female Wistar Rats ◽  
Ad Libitum ◽  
Group 2 ◽  
Group 1

This research aimed to examine the time of onset and sedation on diabetic mellitus (DM) rat (Rattus norvegicus) by propofol. This study used 8 female wistar rats 2-3 months old and 150-200 grams of bodyweight, fed with standard feed and water ad libitum. Samples were divided into two groups. Group 1 (KI) as a control group was injected intraperitoneally with aloxan solvent and group 2 (KII) was injected intraperitoneally with 150 mg/kg bodyweight aloxan. Blood glucose was checked 10 days after aloxan injection, DM was categorized if the blood glucose over 150 mg/dl. All rats were injected intravenously with propofol 1% with the dose of 10 mg/kg bodyweight. Onset and sedation time was counted using stopwatch after propofol injection. The result showed that mean (±SD) of onset on KI and KII were 1.39±0.49 and 8.64±1.23 seconds, consecutively and they were significantly different at P0.01. Whilst mean (±SD) of sedation KI and KII were 12.12±1.47 minutes and 7.62±1.61 minutes, respectively. The onset and sedation time of KI was significantly different from KII P0.01. The conclusion of this research was DM affects time of onset and sedation when wistar strain rats were anesthetized by 1% propofol.Key words: rats, diabetic mellitus, propofol, onset, sedation

scholarly journals Pengaruh Pemberian Etanol Peroral terhadap Berat Testis Tikus Putih (Rattus norvegicus Strain Wistar)

2018 ◽  
Vol 2 (1) ◽  
pp. 7-11
Author(s):  
Antonius Oktavian ◽  
Elieser Elieser ◽  
Agnes S. Rahayu ◽  
Dais Iswanto
Keyword(s):  
Rattus Norvegicus ◽  
Reproductive Organ ◽  
Male Reproduction ◽  
Male Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Significant Difference ◽  
The Difference ◽  
Group 2 ◽  
Level Of Significance

Alcohol (ethanol) has long been used as liquor. In some areas in Indonesia, the use of ethanol is an insaperable part of family rites and traditional festivities.  However, due to its serious consequences, alcoholism becomes a chronic social problem. Ethanol effects the metabolism in body organs and tissues, including male reproductive organ. The objective of this study was to prove that ethanol comsumption may reduce the weight of testis. This study used Posttest Only Group Design with experimental animals of 30 male rats (Wistar strain Rattus norvegicus) that were subjected in five types of treatment, i.e,(1) feeding with aquades 2ml/day as control group (2) feeding with 10% 1 gr/Kg/day of ethanol, (3) feeding with 10% 3gr/Kg/day of ethanol, (4) feeding with 30% 1 gr/Kg/day of ethanol and (5) feeding with 30% 3 gr/Kg/day of ethanol. Treatment was given daily for 45 days. At the end of the study, on the day 46th, the rats was sacrified for examination. Sample of testis were taken for weight examination.  Data were analysed using Anova and when the difference was found, the analysis was followed with Least significant Difference at the level of significance of 95%. The result of this study showed that ethanol reduced the weight of testis compared to control. The results of Anova  test revealed that higher dosage and concentrations of ethanol resulted in significantly different decrease of the testis’s weight (p<0,05) compared to other dosage and other concetration. In conclusion, ethanol has effect in the reduction of the weight of the testis.Key words: ethanol, male reproduction, Rattus norvegicus, weight of testis.

scholarly journals Protective effect of Toxocara vitulorum extract against alpha-naphthylisothiocyanate-induced cholangitis in rat

2020 ◽  
Vol 81 (1) ◽  
Author(s):  
Abeer Mahmoud Badr ◽  
Mohamed Farid ◽  
Ahmed Abdel Aziz Biomy ◽  
Ayman Saber Mohamed ◽  
Noha Ahmed Mahana ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Wistar Rat ◽  
Liver Function Tests ◽  
T Helper ◽  
Standard Drug ◽  
Toxocara Vitulorum ◽  
Stress Markers ◽  
Tnf Α ◽  
Ifn Γ ◽  
Antioxidant Markers

Abstract Background Cholestasis is the major cause of bile acid accumulation leading to liver damage. Chronic infection of worms can modulate the immune response towards T helper (Th)2-related cytokines. The present study aims to evaluate the protective impact of an ascarid nematode Toxocara vitulorum extract (TvE) against alpha-naphthylisothiocyanate (ANIT)-induced cholangitis male wistar rat model compared to ursodeoxycholic acid (UDCA) as a standard drug. Results Pretreatment with TvE and/or UDCA induced a marked reduction in the levels of liver function tests and malondialdehyde, while antioxidant markers were increased compared to cholestatic rats. Pretreatment with either TvE or combination before cholangitis induction attenuated the predominant Th1-related cytokines (IFN-γ and TNF-α) to Th2 (IL-13 and IL-10). TvE administration promoted higher expression levels of Bcl-2 protein and lower levels of caspase-3 compared to cholestatic rats. Conclusions Treatment with TvE has improved the liver functions and elevated the levels of oxidative stress markers. The upregulation of Th2-related cytokines and suppression of apoptosis through caspase-3 might be considered as a potential mechanism of TvE. Thereby, this natural extract revealed an opportunity for use in treatment of cholangitis disease.

scholarly journals Effects of Silver Nanoparticles on Proliferation and Apoptosis in Granulosa Cells of Chicken Preovulatory Follicles: An In Vitro Study

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1652
Author(s):  
Dorota Katarzyńska-Banasik ◽  
Anna Kozubek ◽  
Małgorzata Grzesiak ◽  
Andrzej Sechman
Keyword(s):  
Silver Nanoparticles ◽  
Granulosa Cells ◽  
Caspase 3 ◽  
In Vitro Study ◽  
Poultry Production ◽  
Cell Death Pathways ◽  
Preovulatory Follicles ◽  
Proliferation And Apoptosis ◽  
Apoptosis Process

The continuous development of poultry production related to the growing demand for eggs and chicken meat makes it necessary to use modern technologies. An answer to this demand may be the use of nanotechnology in poultry farming. One of the promising nanomaterials in this field are silver nanoparticles (AgNPs), which are used as disinfectants, reducing microbial pollution and the amounts of greenhouse gases released. This study aimed to evaluate the effect of AgNPs on the proliferation and apoptosis process in the granulosa cells of chicken preovulatory follicles. The in vitro culture experiment revealed that both 13 nm and 50 nm AgNPs inhibited the proliferation of the granulosa cells. However, a faster action was observed in 50 nm AgNPs than in 13 nm ones. A size-dependent effect of AgNP was also demonstrated for the caspase-3 activity. AgNPs 13 nm in size increased the caspase-3 activity in granulosa cells, while 50 nm AgNPs did not exert an effect, which may indicate the induction of distinct cell death pathways by AgNPs. In conclusion, our study reveals that AgNPs in vitro inhibit granulosa cell proliferation and stimulate their apoptosis. These results suggest that AgNPs may disrupt the final stage of preovulatory follicle maturation and ovulation.

scholarly journals Thrombospondin-1 Inhibits Angiogenesis and Promotes Follicular Atresia in a Novel in Vitro Angiogenesis Assay

Endocrinology ◽  
2010 ◽  
Vol 151 (3) ◽  
pp. 1280-1289 ◽  
Author(s):  
Samantha A. Garside ◽  
Christopher R. Harlow ◽  
Stephen G. Hillier ◽  
Hamish M. Fraser ◽  
Fiona H. Thomas
Keyword(s):  
Granulosa Cells ◽  
Caspase 3 ◽  
Polycystic Ovary ◽  
Dependent Manner ◽  
Ovary Syndrome ◽  
Angiogenesis Assay ◽  
Thrombospondin 1 ◽  
In Vitro Angiogenesis ◽  
Dose Dependent

Thrombospondin-1 (TSP-1) is a putative antiangiogenic factor, but its role in regulating physiological angiogenesis is unclear. We have developed a novel in vitro angiogenesis assay to study the effect of TSP-1 on follicular angiogenesis and development. Intact preantral/early antral follicles dissected from 21-d-old rat ovaries were cultured for 6 d in the presence or absence of TSP-1. At the end of the culture period, angiogenic sprouting from the follicles was quantified using image analysis. Follicles were fixed and sectioned, and follicular apoptosis was assessed by immunohistochemistry for activated caspase-3 in granulosa cells. The results showed that TSP-1 inhibited follicular angiogenesis (P &lt; 0.01) and promoted follicular apoptosis (P &lt; 0.001) in a dose-dependent manner. To determine whether the proapoptotic activity of TSP-1 is mediated by direct effects on granulosa cells, isolated granulosa cells were cultured with TSP-1 (0, 10, 100, and 1000 ng/ml) for 48 h. Apoptosis was quantified using a luminescent caspase-3/7 assay. TSP-1 promoted apoptosis of granulosa cells in a dose-dependent manner (P &lt; 0.05), suggesting that TSP-1 can act independently of the angiogenesis pathway to promote follicular apoptosis. These results show that TSP-1 can both inhibit follicular angiogenesis and directly induce apoptosis of granulosa cells. As such, it may have potential as a therapeutic for abnormal ovarian angiogenesis and could facilitate the destruction of abnormal follicles observed in polycystic ovary syndrome.

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