scholarly journals STUDY OF SERUM OSTEOPROTEGERIN AND RECEPTOR ACTIVATOR OF NUCLEAR FACTOR KAPPA-Β LIGAND IN PATIENTS WITH RHEUMATIC DISEASES

2021 ◽  
Vol 27 (4) ◽  
pp. 4170-4177
Author(s):  
Mariela Geneva-Popova ◽  
◽  
Stanislava Popova ◽  
◽  
◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Ankylosing Spondylitis ◽  
Bone Metabolism ◽  
Serum Calcium ◽  
Rheumatic Diseases ◽  
Control Group ◽  
Nuclear Factor ◽  
Serum Osteocalcin ◽  
Receptor Activator

Although rheumatological diseases are widespread, they still have an unclear etiology, pathogenesis, and therapy. Researchers are looking for biomarkers associated with inflammatory and degenerative joint diseases. Serum Osteoprotegerin (s-OPG) and serum Receptor Activator of Nuclear Factor Kappa-Β Ligand (s-RANKL) have not been studied in patients with Diffuse idiopathic skeletal hyperostosis (DISH), spondylosis, ankylosing spondylitis, and gout. The aim of this study was to investigate s-OPG and s-RANKL in patients with various rheumatic diseases. Materials and methods: We studied 135 patients with rheumatic diseases, of which 55 were diagnosed with DISH, 50 with spondylosis, 23 with ankylosing spondylitis, 7 with gout, and 25 were a control group. s-OPG and s-RANKL, serum calcium, ionized calcium, serum phosphorus, alkaline phosphatase, uric acid, urea, creatine, and serum osteocalcin were tested using the ELISA method in the Clinical Laboratory of "St. George" University Hospital, Plovdiv. The results were processed using the statistical program SPSS ver 26, with significance p<0.05. Results: Patients with DISH and ankylosing spondylitis had higher levels of s-OPG, s-RANKL, and s-OPG/s-RANKL ratio than the control group (p<0.05). The s-OPG/s-RANKL ratio in patients with spondylosis and gout was lower than in patients with DISH and ankylosing spondylitis (p<0.05). There are strong correlations between s-OPG, s-RANKL, and the biochemical parameters related to bone metabolism (serum calcium, ionized calcium, serum phosphorus, alkaline phosphatase, uric acid, urea, creatine, and serum osteocalcin)(p <0.05). Conclusion: Our studies show that changes in bone metabolism are similar in patients with DISH and ankylosing spondylitis. Further research is needed to look for a common pathogenetic pathway linking degenerative and inflammatory rheumatic diseases of the axial skeleton.

scholarly journals Indicators of bone metabolism in patients of psychiatric clinic on the background of dimephosphone intake

2016 ◽  
Vol 97 (5) ◽  
pp. 669-673
Author(s):  
R Yu Il’ina ◽  
O O Pasynkova ◽  
L R Mukhamedzhanova ◽  
L E Ziganshina
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Mental Disorders ◽  
Bone Metabolism ◽  
Phosphatase Activity ◽  
Serum Calcium ◽  
Alkaline Phosphatase Activity ◽  
Psychiatric Patients ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase

Aim. To study the indicators of bone metabolism in patients of psychiatric clinics on the background of dimephosphone oral intake.Methods. Research was conducted at the Republican Clinical Psychiatric Hospital named after V.M. Bekhterev (Kazan). 64 patients (36 men and 28 women) aged 45 to 68 years (mean age 56.8±6.9 years) with various forms of mental disorders taking psychotropic drugs for the period of 3 years and over were examined. Resorption markers were determined - D-pyridinoline in urine, serum tartrate-resistant acid phosphatase, alkaline phosphatase, and the content of total and ionized serum calcium. All patients were divided into two groups: those who received dimephosphone in the combined systemic therapy and those who did not. The average duration of the dimephosphone administration was 22±4.2 days, the patients undergone on average 2.6±1.44 courses of treatment during 6 months.Results. The activity of tartrate-resistant acid phosphatase in psychiatric patients was higher compared with those of the control group. The maximum value was registered in patients who were not taking dimephosphone. The alkaline phosphatase activity was the highest in mentally healthy patients of the control group. In psychiatric patients treated with dimephosphone, alkaline phosphatase activity was 20% lower compared with the control group. The minimum values of alkaline phosphatase were registered in patients with mental disorders not taking dimephosphone. When studying the concentration of total and ionized serum calcium there were no significant differences between patients of comparison group and psychiatric patients.Conclusion. Shift in processes of bone remodeling toward its resorption indicates a high risk of pathological fractures in psychiatric patients and the need for osteoporosis prevention; identified changes in biochemical markers of osteoporosis on the background of dimephosphone intake allow to recommend it for the treatment of diagnosed osteoporosis in a psychiatric hospital settings.

Areca nut extracts modulated expression of alkaline phosphatase and receptor activator of nuclear factor kappaB ligand in osteoblasts

2005 ◽  
Vol 32 (4) ◽  
pp. 353-359 ◽  
Author(s):  
Li-Jane Ling ◽  
Feng-Chuang Ho ◽  
Yen-Ting Chen ◽  
Douglas W. Holborow ◽  
Tsung-Yun Liu ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Nuclear Factor Kappab ◽  
Nuclear Factor ◽  
Areca Nut ◽  
Receptor Activator

scholarly journals Bone metabolism biomarkers in walking children with cerebral palsy

2020 ◽  
Vol 7 (4) ◽  
pp. 79-86
Author(s):  
Vladimir M. Kenis ◽  
Svetlana L. Bogdanova ◽  
Tatyana N. Prokopenko ◽  
Andrei V. Sapogovskiy ◽  
Tatyana I. Kiseleva
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Cerebral Palsy ◽  
Bone Metabolism ◽  
Control Group ◽  
Study Group ◽  
Flat Feet ◽  
Children With Cerebral Palsy ◽  
Calcium Phosphorus ◽  
Orthopedic Patients

Backgrоund. Osteoporosis is an important factor in the pathogenesis of orthopedic manifestations in children with cerebral palsy. It was previously demonstrated that children with cerebral palsy have specific changes in bone metabolism, which can cause changes in laboratory parameters compared with other orthopedic patients without neurological backgrounds. Aim. The aim of this study was to assess bone metabolism biomarkers in children with cerebral palsy, identifying distinguishing characteristic patterns in comparison with patients with orthopedic pathology without neurological backgrounds. Materials and methods. This study evaluated the concentrations of calcium, phosphorus, -cross laps, osteocalcin, vitamin D, CICP, and alkaline phosphatase in the blood serum of 50 children with cerebral palsy aged between 6 to 12 years with GMFCS levels IIII. The control group consisted of 50 patients with plano-valgus deformities of the feet. Results. The alkaline phosphatase activity in the group of children with cerebral palsy was 170.25 59.35 u/L, while in the control group it was 145.58 46.29 u/L; the CICP concentration in the study group was higher than in the control group (324.01 174.10 and 269.68 240.98, respectively). The concentration of -cross laps, osteocalcin, calcium, and vitamin D in the study group was lower than in children with flat feet. Conclusions. This study demonstrated multidirectional changes in the biomarkers of bone metabolism that are characteristic of walking children with cerebral palsy. These changes are characterized by a corresponding increase in the activity of osteoresorption and osteoreparation. This makes it possible to justify the combined use of metabolites and metabolic activators (calcium and vitamin D) and drugs that suppress osteoresorption (bisphosphonates) for the prevention and treatment of osteoporosis in children with cerebral palsy.

scholarly journals The expression of nuclear factor of activated T cell c1 and receptor activator of nuclear factor kappa β induced by Enterococcus faecalis in osteoclastogenesis (laboratory experiment on Wistar rats)

2019 ◽  
Vol 52 (4) ◽  
pp. 172
Author(s):  
Nirawati Pribadi ◽  
Rosita Rahmawati ◽  
Mandojo Rukmo ◽  
Adelina Kristanti Tandadjaja ◽  
Hendy Jaya Kurniawan ◽  
...  
Keyword(s):  
T Cell ◽  
Enterococcus Faecalis ◽  
Wistar Rats ◽  
Bone Defects ◽  
Control Group ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa ◽  
Endodontic Infection ◽  
Receptor Activator ◽  
Activated T Cell

Background: Enterococcus faecalis (E. faecalis) is the most common bacteria species in persistent endodontic infection of teeth undergoing root canal treatment at a prevalence of 38%. The virulence factor of this bacterium is Lipoteichoic acid (LTA) which can be recognized by Toll-like receptors-4 (TLR-4) that produce a stimulus and provoke an immune response. Inflammation results in bone defects that feature multiple cytokines and interactions between different cell types. Bone loss within a periapical tooth is characterized by osteoclast formation (osteoclastogenesis) in the bone. Purpose: This study aimed to determine the expression of nuclear factor of activated T cell c1 (NFATc1) and receptor activator of nuclear factor kappa β (RANK) which played a role in osteoclastogenesis at different time intervals. Methods: 36 upper molar teeth of the research subjects were induced with 106 CFU Enterococcus faecalis and subsequently observed for 7 and 21 days with the NFATc1 and RANK being counted microscopically at 1000X magnification across 20 viewing fields. Thereafter, the data was examined and analyzed by means of an independent T test using SPSS. Results: NFATc1 and RANK expression were higher in the group including E. faecalis on days 7 and 21 than in the control group. There were significant differences between the treatment group and control group with regard to NFATc1 and RANK expression (p<0.05). Conclusion: The study showed that the expression of NFATc1 and RANK, which plays a role in osteoclastogenesis, was higher in periapical bone defects in Wistar rats induced by E. faecalis than those which were not induced.

Effect of Alendronate Treatment on Salivary Levels of Osteoprotegrin and TNF-α in Postmenopausal Woman with Osteoporosis and Periodontal Diseases

2018 ◽  
Vol 30 (2) ◽  
pp. 17-22
Author(s):  
Aseel J. Ibraheem ◽  
Aysar N. Mohammed
Keyword(s):  
Bone Resorption ◽  
Postmenopausal Women ◽  
Nuclear Factor Kappa B ◽  
Periodontal Diseases ◽  
Control Group ◽  
Nuclear Factor ◽  
Decoy Receptor ◽  
Tnf Α ◽  
Receptor Activator ◽  
Alendronate Treatment

Background: All diseases concerning bone destruction such as osteoporosis and periodontal diseases share common pattern in which the osteoclast cells are absolutely responsible for bone resorption that occurred when osteoclast activity exceeds osteoblast activity. Osteoprotegrin (OPG) considered as novel soluble decoy receptor known as “bone protector” since it prevents extreme bone resorption through inhibition of differentiation and activity of osteoclast by competing for binding site. It binds to receptor activator of nuclear factor kappa-B ligand (RANKL) and prevent its interaction with receptor activator of nuclear factor kappa-B (RANK), thus inhibits osteoclast formation. TNF-α is a pro-inflammatory cytokines having a broad range of important roles in regulation of immune system and bone resorption through the stimulation of osteoclastogenesis. Alendronate (ALN) diminishes the expression of osteoclast activating factors and cytokines such as RANKL and enhances the production of decoy receptor osteoprotegerin in osteoblast cells. Moreover, it decreases the production of proinflammatory cytokines such as TNF-α by macrophage, stimulates apoptosis of monocyte-macrophage cell lines derivative and reduces inflammatory response. Aims of the Study: 1. To assess the effect of alendronate treatment on salivary levels of osteoprotegrin and TNF-α in postmenopausal women with osteoporosis and periodontal disease 2. To find any possible correlation between salivary levels of osteoprotegrin and TNF-α in control and study groups. Materials and Methods: Total sample of 90 female subjects (55-65 years) were divided into 3 groups, (30 subjects in each group): first control group involved systemically healthy subjects with healthy periodontium, second group involved postmenopausal women with osteoporosis under alendronate treatment for(3-6)months (alendronate group), third group involved postmenopausal women with osteoporosis without alendronate treatment(osteoporosis group). The last two groups were sub- divided in- to two sub –groups (15 subjects in each sub-group) of gingivitis and periodontitis subjects respectively. Salivary samples were collected from all subjects and salivary levels of osteoprotegrin and TNF- α were determined by enzyme –linked immune sorbent assay (ELISA). Results: Highest median value of salivary (OPG) was found in alendronate group followed by control group while the lowest value was found in osteoporosis group. Highest median value of TNF- α was found in osteoporosis group followed by control group and alendronate group respectively with highly significant differences between them. Spearman correlation between salivary levels of TNF-α and OPG showed non- significant correlation at all subgroups. Conclusion: Subjects with osteoporosis in this study had greater levels of TNF-α and decrease in the level of OPG comparing with patients under alendronate treatment. Alendronate treatment for women with osteoporosis and periodontal disease may have beneficial outcome.

scholarly journals Bone Density, Osteocalcin and Deoxypyridinoline for Early Detection of Osteoporosis in Obese Children

2015 ◽  
Vol 3 (3) ◽  
pp. 413-419 ◽  
Author(s):  
Ghada El-Dorry ◽  
Hala Ashry ◽  
Tarek Ibrahim ◽  
Tahany Elias ◽  
Fatma Alzaree
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Density ◽  
Serum Calcium ◽  
Negative Correlation ◽  
Total Mass ◽  
Serum Alkaline Phosphatase ◽  
Significant Negative Correlation ◽  
Whole Body ◽  
Serum Osteocalcin ◽  
Obese Children

AIM: This study aimed at comparing between bone density using DEXA, serum osteocalcin and urinary DPD in obese and non obese prepubertal children. METHODS: After taking the consent of eighty children they were subjected to: full examination, anthropometric measurements, blood samples were withdrawn to determine serum osteocalcin, Ca, Ph, alkaline phosphatase, and urinary DPD. Bone densities, body composition of the whole body were examined using DEXA. Data were analyzed using SPSS.RESULTS: All anthropometric variables showed significant increase in obese children except for height in comparison to control group. Total mass, lean + BMC, lean, fat, area, BMC, BMD and Z score of the whole body were significantly increased in obese children. Serum calcium showed significant increase while alkaline phosphatase was significantly decreased in obese children. DPD showed no significant difference between obese and non obese children. Significant negative correlation was found between ca, lean, lean + BMC and total mass. Serum alkaline phosphatase showed also a significant negative correlation with (lean + BMC and total mass). Serum osteocalcin showed negative significant correlation with area, BMC, BMD, lean and Z score.CONCLUSION: Obese children showed significant increase in anthropometric and DEXA parameters, increase in serum calcium and significant decrease in serum alkaline phosphatase. Osteocalcin was negatively correlated with most of DEXA results.

scholarly journals Serum uric acid, lipid profile and alkaline phosphatase levels in ischemic cerebrovascular accident patients

2021 ◽  
Vol 8 (8) ◽  
pp. 1171
Author(s):  
Gaurav Gupta ◽  
Saurabh Kishor ◽  
Aditya Kumar
Keyword(s):  
Alkaline Phosphatase ◽  
Uric Acid ◽  
Ischemic Stroke ◽  
Serum Uric Acid ◽  
Cerebrovascular Accident ◽  
Serum Alkaline Phosphatase ◽  
Multiple Logistic Regression Analysis ◽  
Elevated Serum ◽  
Control Group ◽  
Significant Difference

Background: Stroke or cerebrovascular accident (CVA) is noted as the second cause of mortality, especially in the elderly population. Recent studies indicated that higher concentrations of uric acid are involved in various vascular diseases. The findings of previous investigations suggest that, elevated serum alkaline phosphatase (ALP) levels may have a pathophysiological character in the occurrence of atherosclerotic vascular disease (AVD) of the heart and brain. This study evaluated the association between serum uric acid (SUA) levels, serum lipid levels, serum alkaline phosphatase (ALP) levels, and changes in ischemic cerebrovascular accident patients.Methods: All patients with Ischemic cerebrovascular accident age >50 years were included based on their clinical, laboratory, and radiological findings (including computed tomography (CT)/magnetic resonance imaging (MRI)) those admitted in our hospital. As control group 200 healthy individuals matched for sex and age were recruited from the same demographic area.Result: Multiple logistic regression analysis findings proposed four components as significant predictors in ischemic cerebrovascular accident (serum uric acid, serum ALP, LDL and HDL. In this study, it was found, that patients with ischemic cerebrovascular accident had significant difference (p<0.001) in serum uric acid and serum ALP than normal patients (non-ischemic cerebrovascular accident patients).Conclusions: Patients with ischemic cerebrovascular accident had significant difference (p<0.001) in SUA and serum ALP than normal patients (non-ischemic cerebrovascular accident patients). High SUA levels were observed to be associated significantly with ischemic stroke. On the basis of our study design, we cannot clarify that the elevated levels are the risk of ischemic stroke and it requires further studies.

Osteoprotective Effect of Cimiracemate in Glucocorticoid-Induced Osteoporosis by Osteoprotegerin/Receptor Activator of Nuclear Factor κ B/Receptor Activator of Nuclear Factor Kappa-Β Ligand Signaling

Pharmacology ◽  
2019 ◽  
Vol 103 (3-4) ◽  
pp. 163-172
Author(s):  
Zheng Ding ◽  
Huifeng Shi ◽  
Wei Yang
Keyword(s):  
Bone Marrow ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Nuclear Factor ◽  
Methylprednisolone Acetate ◽  
Histopathological Changes ◽  
Mediators Of Inflammation ◽  
Receptor Activator ◽  
Nuclear Factor Κ B

Objectives: Present investigation determines the protective effect of cimiracemate A against glucocorticoid-induced osteoporosis rat. Methods: Osteoporosis was induced by injecting methylprednisolone acetate (21 mg/kg) for the period of 6 weeks, and the rats were treated with cimiracemate A 5 and 10 mg/kg, p.o. 60 min after the administration of methylprednisolone acetate (21 mg/kg) for the duration of 6 weeks. Effect of cimiracemate A was observed by estimating the microarchitecture of bone and histopathological changes by micro-CT scan and light microscope. Moreover, lipid profile, mediators of inflammation, and parameters that affect bone formation were determined in the serum and western blot assay, and reverse transcription polymerase chain reaction was done for the estimation of protein expression in the bone tissues. Moreover, cytotoxic effect of cimiracemate A on bone marrow macrophages and bone marrow stromal cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: Result of the investigation suggests that treatment with cimiracemate A ameliorates the microarchitecture of bone and histopathological changes in the glucocorticoid-induced osteoporosis rat. Level of lipid and mediators of inflammation was significantly reduced in the serum of cimiracemate A-treated rats than the negative control group. However, the activity of tartrate-resistant acid phosphatase and the level of collagen type I fragments in the serum were found to be reduced, and osteocalcin level was enhanced in cimiracemate A-treated rats than the negative control group. Moreover, treatment with cimiracemate A attenuates the expression of receptor activator of nuclear factor kappa-Β ligand (RANKL), receptor activator of nuclear factor κ B (RANK), and osteoprotegerin (OPG) protein in glucocorticoid-induced osteoporosis rats. Conclusion: In conclusion, our study suggests that cimiracemate A protects the glucocorticoid-induced osteoporosis by regulating the RANKL/RANK/OPG signaling pathway.

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