The study of acute and subchronic toxicities of Da Dai Trang HVD capsules in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 7-15
Author(s):  
Pham Thi Van Anh ◽  
Nguyen Van Dam ◽  
Pham Thanh Ky ◽  
Vu Viet Hang ◽  
Dinh Thi Thu Hang
Keyword(s):  
Acute Toxicity ◽  
Deleterious Effect ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Renal Functions ◽  
Swiss Mice ◽  
Human Dose ◽  
Oral Doses

The purpose of this research is to evaluate the acute and subchronic toxicities of DA DAI TRANG HVD capsules through oral administration in experimental animals. The acute toxicity was determined by the method of Litchfield Wilcoxon in Swiss mice. The subchronic toxicity was evaluated by the recommendation of WHO and OECD in Wistar rats with oral doses of 1.44 g/kg/day (equal to recommended human dose) and 4.32 g/kg/day (3 times as high as recommended human dose) in 4 consecutive weeks. As a result, DA DAI TRANG HVD capsules at the highest dose used for mice (99.9 g materials/kg) did not express acute toxicity in mice. In term of the subchonic toxicity test, DA DAI TRANG HVD had no deleterious effect on hematological parameters, hepato-renal functions, macroscopic and microscopic images of livers and kidneys of rats. In conclusion, DA DAI TRANG HVD capsules did not produce the acute and subchronic toxicities in Swiss mice and Wistar rats.

The evaluation of acute and subchronic toxicities of An Phu Khang capsules in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 86-95
Author(s):  
Ha Thi Yen ◽  
Tran Thanh Tung ◽  
Dang Thi Thu Hien
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Swiss Mice ◽  
Human Dose ◽  
Liver And Kidney

The purpose of this research was to evaluate the acute and subchronic toxicities of An Phu Khang capsules through oral administration in experimental animals. The acute toxicity was determined by the method of Litchfield Wilcoxon in Swiss mice. The subchronic toxicity was evaluated by the recommendation of WHO in Wistar rats at these doses of 0.54 g/kg b.w/day (equal to recommended human dose) and 1.62 g/kg b.w/day (3 times as high as recommended human dose) in 4 consecutive weeks. As a result, An Phu Khang capsules at the highest dose used for mice (36.29 g/kg b.w) did not show acute toxicity in mice. In terms of the subchronic toxicity test, after oral administration of An Phu Khang capsules, hematological parameters, hepato-renal functions, and microscopic images of liver and kidney at both doses were unchanged compared with the control group. In conclusion, An Phu Khang with both doses 0.54 g/kg b.w/day and 1.62 g/kg b.w/day did not produce acute and subchronic toxicities in Swiss mice and Wistar rats.

scholarly journals Evaluation of acute and subchronic toxicities of “Phuong Dong Dai Trang” tablets in experimental animals

2021 ◽  
Vol 141 (5) ◽  
pp. 29-38
Author(s):  
Tran Thanh Tung ◽  
Dau Thuy Duong ◽  
Pham Thi Thuy Minh ◽  
Nguyen Thu Hien ◽  
Dinh Thi Thu Hang
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Swiss Mice ◽  
Human Dose ◽  
Liver And Kidney ◽  
Oral Doses

The study aimed to evaluate the acute and subchronic toxicities of “Phuong Dong Dai Trang” tablets through oral administration using experimental animal models. Acute toxicity in Swiss mice was determined using the Litchfield Wilcoxon method. The subchronic toxicity in Wistar rats was evaluated according to WHO and OECD’s recommendation with oral doses of 4.68 g/kg/day (equivalent to recommended human dose) and 14.04 g/kg/day (3 times the recommended human dose) for 4 consecutive weeks. In terms of acute toxicity, “Phuong Dong Dai Trang” tablets did not express acute toxicity in mice at the highest dose used (232.14 g materials/kg). In terms of the subchronic toxicity, after oral administration of “Phuong Dong Dai Trang” tablets, hematological parameters, hepato - renal functions, and microscopic images of liver and kidney were unchanged in the treatment group compared to the control group. In conclusion, “Phuong Dong Dai Trang” tablets did not produce acute and subchronic toxicities in Swiss mice and Wistar rats.

Evaluation of acute and subchronic toxicity of “Tran Chau Nguu Hoang Hoan” in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 38-47
Author(s):  
Tran Thai Ha ◽  
Pham Thi Van Anh ◽  
Dao Xuan Tinh ◽  
Dinh Thi Thu Hang
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Toxicity Test ◽  
Acute Toxicity Test ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Microscopic Images ◽  
Renal Functions ◽  
Liver And Kidney ◽  
Oral Doses

“Tran chau nguu hoang hoan” was prepared from 12 herbal ingredients. So far, the safety of this product, has not been reported yet. Thus, this study aimed to evaluate the acute and subchronic toxicity of “Tran chau nguu hoang hoan” through oral administration in experimental animals. The acute toxicity was determined by the method of Litchfield Wilcoxon in mice at the doses of 2.42 g/kg b.w/day to 6.04 g/kg b.w/day. The subchronic toxicity was evaluated followed the Guideline of WHO and OECD in rats with oral doses of 58.0 mg/kg b.w/day and 174.0 mg/kg b.w/day for 12 consecutive weeks. As a result, in the course of the acute toxicity test, the mice showed no abnormal sign or death. In terms of the subchonic toxicity test, hematological indexes, hepato-renal functions and microscopic images of liver and kidney were unchanged. In conclusion, “Tran chau nguu hoang hoan” does not appear to produce acute and subchronic toxicities in mice and rats.

Acute and subchronic oral toxicity assessments of BTL tea in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 48-57
Author(s):  
Tran Thai Ha ◽  
Bui Viet Chung ◽  
Pham Thi Van Anh ◽  
Nguyen Thi Ngoc ◽  
Dinh Thi Thu Hang
Keyword(s):  
Acute Toxicity ◽  
Toxicity Test ◽  
Current Trend ◽  
Premature Death ◽  
Zingiber Officinale ◽  
Control Group ◽  
Oral Toxicity ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Human Dose

Tobacco smoking remains a leading cause of preventable diseases and premature death in many countries. Many smokers want to quit smoking but are not offered the highly effective treatments available to manage tobacco dependency. There has been a current trend for researchers to find new natural ingredients that were safe and still effective in treating tobacco dependence. BTL tea was a herbal-derived product prepared from Herba Menthae, Pogos cablin (Blanco) Benth., Zingiber Officinale Rosc., Flos Chrysanthemi, Radix Glycyrrhizae, Pericarpium Citri deliciosa, and Flos Lonicera. At this time, the safety of this product has not been reported. Thus, this study aimed to evaluate BTL tea’s acute and subchronic toxicity through oral administration in experimental animals. The acute toxicity was determined by Litchfield-Wilcoxon method in mice at the doses of 45.0 g/kg b.w/day to 112.5 g/kg b.w/day. The subchronic toxicity was evaluated following WHO and OECD’s Guidelines in rats with oral doses of 1.08 g/kg b.w/day (equal to recommended human dose) and 3.24 g/kg b.w/day (three times as high as recommended human dose) for four consecutive weeks. As a result, in the acute toxicity test, the mice showed no abnormal sign or death. The subchronic toxicity test, hematological indexes, hepato-renal functions, and microscopic images of liver and kidney were unchanged. However, compared with the control group, there were significant differences in various indexes, including total WBC, lymphocytes, neutrophils, and AST level, but the levels were still safe. In conclusion, BTL tea does not appear to produce acute and subchronic toxicities in mice and rats.

scholarly journals Subchronic Toxicity Evaluation of Ethanol Extract of Picria fel-terrae Lour. Leaf in Wistar Rats

2018 ◽  
Vol 86 (3) ◽  
pp. 34
Author(s):  
Urip Harahap ◽  
Yuandani ◽  
Marianne ◽  
Hafiza Agustya ◽  
Dira Azizah ◽  
...  
Keyword(s):  
Toxic Effect ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Ethanol Extract ◽  
Hepatoprotective Activity ◽  
Toxicity Evaluation ◽  
Subchronic Toxicity ◽  
Relative Organ Weight ◽  
Histopathology Examination

Picria fel-terrae extract has been investigated for its hepatoprotective activity. This current study was conducted to evaluate the subchronic toxic effect of Picria fel-terrae Lour. leaf extract. The subchronic toxicity of ethanol extract of P. fel-terrae leaf was evaluated by OECD (Organization for Economic Cooperation and Development) guidelines. The extract at doses of 125, 250, 500, 1000 mg/kg body weight (BW) were orally administered to the test animals for 90 days. Signs of toxicity and mortality were observed for 90 days and 118 days. The biochemistry, hematology, macropathology, relative organ weight and histopathology examination was also performed. The macroscopic and microscopic examinations of liver, lung, kidney, spleen and heart displayed normal color and texture after treatment, with ethanol extract P. fel-terrae leaf at doses of 125 and 250 mg/kg BW. The signs of toxicity, mortality, macroscopic and microscopic changes, as well as increasing biochemistry parameters were observed after treatment, with extract at the dose of 500 and 1000 mg/kg BW. However, the abnormal value of biochemistry parameters was returned to normal when the treatment was stopped. There was no effect on hematological parameters at all doses of extract. The ethanol extract P. fel-terrae leaf is untoxic for long-term use at dose 125 and 250 mg/kg BW. The toxic effect of P. fel-terrae at 500 and 1000 mg/kg BW was reversible.

Acute Toxicity of Methanol Extract of Cola nitida Treatment on Antioxidant Capacity, Hepatic and Renal Functions in Wistar Rats

2016 ◽  
Vol 13 (4) ◽  
pp. 1-6 ◽  
Author(s):  
Ejiofor Emmanuel ◽  
Shirley Ebhohon ◽  
Obike Adanma ◽  
Oriaku Edith ◽  
Ogbonnaya Florence ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Antioxidant Capacity ◽  
Wistar Rats ◽  
Methanol Extract ◽  
Renal Functions ◽  
Cola Nitida

scholarly journals Subchronic Toxicity of Green Algae (Spyrogyra sp.) Ethanolic Extract on Hematologic Parameters

2016 ◽  
Vol 5 (4) ◽  
pp. 484
Author(s):  
Nina Salamah ◽  
Wahyu Widyaningsih ◽  
Hari Susanti ◽  
Anggita Devi ◽  
Anita Wening Sejati ◽  
...  
Keyword(s):  
Green Algae ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Test Group ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Immune Parameters ◽  
Hematology Parameters

<p>Green Algae, an organism with active substance such as phytomelatonin, has potential to be developed as Indonesian traditional medicine. As the long term addition of Green Algae ethanol extract (<em>Ekstrak etanol ganggang hijau</em>, EEGH) influences the hematology system, in this paper, the safety test was done to ensure the safety of its use through subchronic toxicity test of EEGH on the hematology parameters of Wistar rats. The test group consisted of three groups treated with EEGH 100 mg/kg, 200 mg/kg, and 400 mg/kg, while the control group was given by 0.5% CMC-Na, with 8 rats each respectively. By using blood samples taken from orbital sinus on the 29<sup>th</sup> day, common hematologic parameters (erythrocytes, leukocytes, and hemoglobin level), the parameters of hemostasis (platelets, pT, aPTT, BT) and immune parameters (Differential Leukocytes Counts include neutrophils segment, lymphocytes, monocytes, and eosinophils) were finally observed and showed that the 28 days-addition of EEGH increase the hematological parameters of Wistar rats.</p>

scholarly journals Acute and subchronic oral toxicities of “Da day An Chau” tablets in experimental animals

2021 ◽  
Vol 141 (5) ◽  
pp. 1-9
Author(s):  
Tran Thanh Tung ◽  
Dao Viet Hoang ◽  
Pham Thi Van Anh ◽  
Dang Thi Thu Hien
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
World Health Organization ◽  
Biochemical Parameters ◽  
Maximum Dose ◽  
Toxicity Study ◽  
World Health ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Health Organization

In this study, the toxicities of “Da day An Chau” tablets (DDAC) were assessed on experimental animals. To evaluate the acute toxicity on Swiss mice according to World Health Organization Guidance and to determine LD50 refer to the method of Litchfield – Wilcoxon. The subchronic toxicity study of DDAC at two doses (0.58 g/kg/day and 1.74 g/kg/day) was conducted in rats for four consecutive weeks. Evaluation of general conditions and weight of rats during the study period. Rat’s blood was taken for hematological and biochemical evaluations. The livers and kidneys microscopes were evaluated at the end of the experiment. The result revealed that mice were taken up to a maximum dose of 25.71 g/kg with no symptoms of acute toxicity; LD50 of DDAC has not been determined. At two doses, the subchronic toxicity study did not change rats’ body weight, hematological, biochemical parameters, and microscopic of the livers and kidneys during the study period.

scholarly journals Evaluation of Sub-acute and Sub Chronic Toxicity Profile of the Aqueous Leaf Crude Extract of Melanthera Scandens (Schumach & Thonn) in Wistar Rats

2019 ◽  
pp. 1-16
Author(s):  
Daniel Chans Mwandah ◽  
Ibrahim Ntulume ◽  
Adamu Almustapha Aliero ◽  
Kennedy Kiyimba ◽  
Emmanuel Tiyo Ayikobua ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Crude Extract ◽  
Wistar Rats ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Significant Treatment ◽  
Study Results ◽  
Need To Evaluate ◽  
Female Wistar Rats ◽  
Male Wistar Rats

Aims: Although Melanthera scandens is a plant widely used in traditional medicine for the management of seizures, stomach ulcers and sores, dysmenorrhea, diabetes and malaria, there was scanty information about its safety. There was, therefore, a need to evaluate the sub-acute and subchronic toxicity studies of this plant which would reflect on its safety. Methodology: This was an experimental laboratory study. The research was conducted at Kampala International University-Western Campus at the Pharmacology laboratory from February to June 2017. The sub-acute toxicity was evaluated after administering daily oral doses of M. scandens crude extract (250, 500 and 1000 mg/kg) for 28 days and 90 days for subchronic study, after which the effect on haematological, biochemical and histopathological parameters were assessed in male and female Wistar rats (five of each sex). Results: Sub-acute toxicity results revealed that there was a significant decrease in the AST between the male Wistar rats that received 250 mg/kg (P= .005) and those that received 500 mg/kg (P= .05) as compared with the control group. Subchronic studies showed a significant increase in ALP (P= .05) at 1000 mg/kg compared with 500 mg/kg. Terminal necropsy did not reveal any treatment-related histopathological findings. There were also no toxicologically significant treatment-related effects on haematological parameters. The sub-acute toxicity results suggest that doses of 250mg/kg and 500mg/kg are safe and could be hepatoprotective due to reduced levels of AST and ALP, while the subchronic toxicity study results suggest that doses greater than 1000 mg/kg could be toxic to the plasma membrane, liver cells or endoplasmic reticulum due to increased ALP levels at this dose. Conclusion: The M. scandens crude extract did not cause significant toxicity on haematological and histopathological indices, after sub-acute and subchronic administration in Wistar rats.

The Effects of Ammonium Molybdate on Renal Function in the Rats after Parathyreoidectomy

10.12737/2733 ◽  
2013 ◽  
Vol 20 (4) ◽  
pp. 71-73
Author(s):  
Меликова ◽  
E. Melikova ◽  
Брин ◽  
Vadim Brin
Keyword(s):  
Renal Function ◽  
Wistar Rats ◽  
Filtration Rate ◽  
Ammonium Molybdate ◽  
Human Organism ◽  
Urine Osmolarity ◽  
Water Reabsorption ◽  
Experimental Animals ◽  
Renal Functions ◽  
Pathogenic Effects

The constant pollution of the environment increases the content of xenobiotics in the air, soil, water and contributes to their entry into the organism. Increased content of molybdenum in the environment has pathogenic effect on the human organism. It is known that the excretion of metals is mainly via the kidney, while the content of the xenobiotics in the urine may have pathogenic effects on renal function. It is of interest to study the renal functions in conditions of inflow metals in the organism. The aim of this work was to study the effect of experimental hypocalcaemia on uropoisis renal function at the chronic molybdenum intoxication. Materials and methods. Experiments were carried out on Wistar-rats. Chronic molybdenum intoxication in experimental animals was caused by intragastric injection of ammonium molybdate solution in the dose of 50mg/kg. during 1 month. Experimental hypocalcaemia was created by the parathyroidectomy. Results. It was noted that when a stand-alone injection of ammonium molybdate glomerular filtration rate is reduced. In the model combined with parathyroidectomy this indicator comes back to normal. Tubular water reabsorption, urinary excretion of calcium and protein are less pronounced than in the isolated introduction ammonium molybdate. Urine osmolarity also had a tendency to decrease. Conclusions. Experimental hypocalcaemia reduces the renal manifestations of chronic molybdenum intoxication.

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