ANALYSIS OF OXIDATIVE STRESS MARKERS IN CROSSBRED COWS WITH SUBCLINICAL MASTITIS AND CONCURRENT METABOLIC AND INFECTIOUS DISEASES

The aim of this study was to evaluate the oxidative status in crossbred cows with subclinical mastitis and concurrent metabolic and infectious diseases. The crossbred cows were groped asGp-C (n=6) as controls,Gp-M (n=6) with clinical mastitis ,Gp-TS consists cows affected withstenosis of teat,Gp-RA with ruminal acidosis,Gp-ND with diarrhea along with dehydration and loss of appetite, Gp-RTIwith coughing, fever, decreased appetite, varying degrees of dyspnea, GpRB with a history of failure to conceive after at least two successive inseminations. oxidative stress parameters likecatalase, SOD, GPx, GSH and malondialdehyde were estimated spectrophotometrically. The antioxidant enzymes like catalase, super oxide dismutase (SOD), Glutathione Peroxidase (GPx) levels were significantly declined and the non enzymatic parameters reduced glutathione (GSH) and malondialdehyde (MDA) are significantly elevated in affected animals than in healthy animals. The results of the oxidative stress parameters indicate imbalance of antioxidant profile and oxidative stress in the animals with various disorders and previous illness along with subclinical mastitis.

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Effect of supplementation of several coated vitamins and chelated trace minerals on reducing the incidences of subclinical mastitis in crossbred cows

Indian Journal of Animal Research ◽

10.18805/ijar.11427 ◽

2016 ◽

Author(s):

B. M. Bhanderi ◽

Ajay Goswami ◽

M. R. Garg

Keyword(s):

Neutrophil Count ◽

Subclinical Mastitis ◽

Clinical Mastitis ◽

Trace Minerals ◽

Control Group ◽

Normal Range ◽

Crossbred Cows ◽

History Of ◽

Vitamins E And A ◽

Experimental Group

Two hundred and fifty five crossbred cows were selected, having a history of sub-clinical mastitis (SCM) and clinical mastitis (CM) in previous lactation. Seventy eight crossbred cows were served as control, and the remaining were fed daily 10 g supplement containing chelated Cu, Zn, Cr, vitamins E and A along with iodine for 4 weeks prior to calving. Milk and blood samples were collected for analysis of SCC, Na, K, Cl, pH, EC, neutrophil count, FRAP activity etc. Out of 177 experimental cows, only 36 (20%) showed signs of SCM and CM with Mastect strip and CMT which was confirmed by SCC in milk (average 7.26x105 cells/ml milk). However, 67 animals (86%) out of 78 in control group were affected by SCM as indicated by the higher SCC in milk (average 10.11x105 cells/ml milk), which was later aggravated to CM. Milk pH, EC, Na and Cl content in milk were higher (P is less than 0.05) in animals affected by SCM than the normal animals. In experimental group, of 141 animals SCC was within the normal range (1.22-2.36x105 cells/ml milk) and no signs of SCM or CM were observed. Milk lactose, protein, SNF and FRAP activity were higher in unaffected as compared to mastitic animals. On supplementation of vitamins and chelated minerals, blood neutrophil count decreased, whereas, immunoglobulin and FRAP activity increased significantly (P is less than 0.05). It was found that feeding vitamins and chelated minerals based supplement during pre-partum period could significantly reduce the incidences of SCM and CM in crossbred cows.

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Amelioration of Oxidative Stress Through Supplementation of Self- formulated Anti-oxidant Mixture for Early Recovery and Prophylaxis of Bovine Mastitis in Kashmir Valley

Indian Journal of Animal Research ◽

10.18805/ijar.b-4339 ◽

2021 ◽

Author(s):

A. Muhee ◽

H.U. Malik ◽

R.A. Bhat ◽

Z.A. Akhoon ◽

Adil Mehraj Khan ◽

...

Keyword(s):

Oxidative Stress ◽

Bovine Mastitis ◽

Clinical Mastitis ◽

Kashmir Valley ◽

Trace Mineral ◽

Oxidative Stress Parameters ◽

Group I ◽

Anti Oxidant ◽

Group Ii ◽

Stress Parameters

Background: During lactation, mammary epithelial cells exhibit a high metabolic rate and thus produce large amounts of ROS and lipid peroxides in vivo (Jin et al. 2014; Ganguly et al., 2016). A surplus of ROS and the absence of optimal amounts of antioxidants (which neutralize these free radicals or ROS) results in oxidative stress (Lykkesfeldt and Svendsen, 2007). A clinical study was undertaken on Bovine Mastitis in Kashmir valley to study the relation between oxidative stress and clinical mastitis. An attempt was also made to see the effect of supplementation of self-formulated anti-oxidant trace mineral mixture on recovery and prophylaxis of Bovine mastitis through amelioration of oxidative stress.Methods: The oxidative stress was assessed through estimation of superoxide dismutase (SOD), catalase, reduced glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO). In addition, blood trace mineral status for copper (Cu), zinc (Zn), manganese (Mn) and selenium (Se) were also assessed in mastitic animals and compared with normal healthy lactating animals. The utility of anti-oxidants in clinical management of mastitis was measured through response to treatment with trace minerals like Cu, Zn, Mn and Se in addition to conventional antibiotic therapy. Two groups of mastitic animals received two therapeutic regimens. Group I animals received antibiotics and self formulated anti-oxidant mixture at therapeutic doses while as Group II animals received only antibiotics (at same dose rate and frequency). Clinical recovery was assessed on the basis of CMT point score, milk somatic cell count and milk biochemistry. For prophylactic study, forty recently parturated lactating animals having susceptibility to occurrence of mastitis were divided into two groups of twenty animals each. One group of animals was supplemented with self-formulated anti-oxidant mixture at prophylactic doses for a period of thirty days so as to see the effect of supplementation on oxidative stress parameters and occurrence of clinical mastitis.Result: A significant decrease was found in the values of SOD, catalase, GSH and Cu, Zn, Mn and Se but a significant increase was found in the values of MDA and NO in clinical cases of mastitis as compared to healthy control group. Therapeutic regimen I proved efficacious than the therapeutic regimen II in treatment of clinical mastitis with higher recovery rates and lesser number of mean days required for recovery in group I than group II animals. The efficacy of prophylactic treatment was monitored by occurrence of mastitis during the course of therapy and one month after therapy. Group I animals did not suffer from clinical mastitis and showed considerable improvement in oxidative stress parameters, milk SCC and blood trace mineral status as compared to group II.

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Presentation of cytokine profile in relation to oxidative stress parameters in patients with severe COVID-19: an observational pilot study

F1000Research ◽

10.12688/f1000research.55166.1 ◽

2021 ◽

Vol 10 ◽

pp. 719

Author(s):

Marija Petrushevska ◽

Dragica Zendelovska ◽

Emilija Atanasovska ◽

Aleksandar Eftimov ◽

Katerina Spasovska

Keyword(s):

Oxidative Stress ◽

Care Setting ◽

High Sensitivity ◽

Photometric System ◽

Oxidative Stress Parameters ◽

Stress Markers ◽

Tnf Α ◽

Array Technology ◽

Ifn Γ ◽

Stress Parameters

Introduction: COVID-19 can be worsened by hyper-production of cytokines accompanied by increased level of oxidative stress. The aim of this study was to investigate the correlation between a set of cytokines and the markers of the oxidative stress. Methods: The levels of cytokines IL-2, IL-4, IL-6, IL8, IL-10, VEGF, IFN-γ, TNF-α, IL-1α, MCP-1 and EGF were determined by using High Sensitivity Evidence Investigator™ Biochip Array technology. The oxidative stress parameters (d-ROM, PAT, OS index) were measured in serum on FRAS5 analytical photometric system. Results: IL-6, IL-8, IL-10, VEGF, MCP-1 and EGF were significantly higher (p<0.05) in the patients with severe COVID-19 with increased levels of IL-2, IFN-g, TNF-a and IL-1α. The d-ROM, OS index, and PAT were significantly higher (p<0.05) in severe COVID-19 patients. IL-6 demonstrated the strongest correlation with all of the markers of the oxidative stress, d-ROM (r=0.9725, p=0.0001), PAT (r=0.5000, p=0.0001) and OS index (r=0.9593, p=0.012). Similar behavior was evidenced between IFN-g and d-ROM (r=0.4006, p=0.0001), PAT (r=0.6030, p=0.0001) and OS index (r=0.4298, p=0.012). Conclusion: The oxidative stress markers show good correlation with the tested cytokines which can be measured at the beginning of the disease in a primary care setting to predict the course of COVID-19.

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Influence of Synbiotics on Selected Oxidative Stress Parameters

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/9315375 ◽

2017 ◽

Vol 2017 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Paulina Kleniewska ◽

Rafał Pawliczak

Keyword(s):

Oxidative Stress ◽

Lactobacillus Casei ◽

Positive Influence ◽

Control Groups ◽

Oxidative Stress Parameters ◽

Stress Markers ◽

Sh Group ◽

Synbiotic Supplementation ◽

And Control ◽

Stress Parameters

The aim of the present study was to assess synbiotic (Lactobacillus casei+ inulin) influence on oxidative stress parameters such as concentrations of malondialdehyde (MDA), hydrogen peroxide (H2O2), glutathione, and free sulfhydryl groups content. Experiments were carried out on healthy volunteers (n=32). The subjects were divided into women group (n=16) and men group (n=16) and randomly assigned to synbiotic and control groups. Blood samples were collected before synbiotic supplementation and after 7 wks, at the end of the study. The administration of synbiotic resulted in a significant decrease in MDA (p<0.01), H2O2(p<0.01), and GSSG concentrations (p<0.05) as compared with the control groups and significant increase in the concentrations of GSHt (p<0.001), GSH (p<0.01), and -SH group content (p<0.05) versus control. Synbiotics containingL. caseiplus inulin may have positive influence on selected oxidative stress markers.

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Effects of Spiruline (Spirulina platensis) Supplementation on Oxidative Stress Markers, Biochemical Characteristics, and Hematological Parameters in Rabbit (Oryctolagus cuniculus) Does

Asian Journal of Agriculture and Rural Development ◽

10.18488/journal.ajard.2021.112.163.170 ◽

2021 ◽

Vol 11 (2) ◽

pp. 163-170

Author(s):

Chongsi Margaret Mary Momo ◽

Mahamat Tahir Markhous Adam ◽

Tchoffo Herve ◽

Vemo Bertin Narcisse ◽

Ngoula Ferdinand ◽

...

Keyword(s):

Oxidative Stress ◽

Hematological Parameters ◽

Control Group ◽

Oxidative Stress Markers ◽

Feed Supplementation ◽

Biochemical Characteristics ◽

Oxidative Stress Parameters ◽

Stress Markers ◽

Sexually Mature ◽

Stress Parameters

The present study was carried out in order to evaluate the effects of feed supplementation with spiruline powder on the oxidative stress markers, biochemical characteristics, and hematological parameters in rabbit doe. Twenty-one nulliparous and sexually mature does (7–8 months old) were distributed into three groups of seven does each, comparable in terms of body weight. After a week of feeding with experimental feed, does of each group were mated. During the trial, does of group 1 (control group) were fed ad libitum with a feed free from spiruline (control feed), while those of groups 2 and 3 received the control feed supplemented with spiruline powder at 0.6 and 1.2%, respectively. Immediately after parturition, blood was collected for analyses of hematological, biochemical, and oxidative stress parameters. Studied parameters included serum concentrations of total proteins, cholesterol, aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), creatinine, and urea; malondialdehyde, reduced glutathione, catalase, and superoxide dismutase; and hematological parameters. The total protein concentration, number of white and red blood cells, platelets, hemoglobin, hematocrit, and mean corpuscular volume were significantly (P<0.05) higher in does treated compared to control. Oxidative stress parameters were comparable (P>0.05) among treatments. Hence, these results show that feed supplementation with spirulina powder can improve rabbit doe health, notably at 1.2%, since optimal results were obtained at this percentage.

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Presentation of cytokine profile in relation to oxidative stress parameters in patients with severe COVID-19: a case-control pilot study

F1000Research ◽

10.12688/f1000research.55166.2 ◽

2021 ◽

Vol 10 ◽

pp. 719

Author(s):

Marija Petrushevska ◽

Dragica Zendelovska ◽

Emilija Atanasovska ◽

Aleksandar Eftimov ◽

Katerina Spasovska

Keyword(s):

Oxidative Stress ◽

Care Setting ◽

High Sensitivity ◽

Photometric System ◽

Oxidative Stress Parameters ◽

Stress Markers ◽

Tnf Α ◽

Array Technology ◽

Ifn Γ ◽

Stress Parameters

Introduction: COVID-19 can be worsened by hyper-production of cytokines accompanied by increased level of oxidative stress. The aim of this study was to investigate the correlation between a set of cytokines and the markers of the oxidative stress. Methods: The levels of cytokines IL-2, IL-4, IL-6, IL8, IL-10, VEGF, IFN-γ, TNF-α, IL-1α, MCP-1 and EGF were determined by using High Sensitivity Evidence Investigator™ Biochip Array technology. The oxidative stress parameters (d-ROM, PAT, OS index) were measured in serum on FRAS5 analytical photometric system. Results: IL-6, IL-8, IL-10, VEGF, MCP-1 and EGF were significantly higher (p<0.05) in the patients with severe COVID-19 with increased levels of IL-2, IFN-y, TNF-α and IL-1α. The d-ROM, OS index, and PAT were significantly higher (p<0.05) in severe COVID-19 patients. IL-6 demonstrated the strongest correlation with all of the markers of the oxidative stress, d-ROM (r=0.9725, p=0.0001), PAT (r=0.5000, p=0.0001) and OS index (r=0.9593, p=0.012). Similar behavior was evidenced between IFN-y and d-ROM (r=0.4006, p=0.0001), PAT (r=0.6030, p=0.0001) and OS index (r=0.4298, p=0.012). Conclusion: The oxidative stress markers show good correlation with the tested cytokines which can be measured at the beginning of the disease in a primary care setting to predict the course of COVID-19.

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