EVALUATION OF IN-VITRO ACTIVITY OF AERIAL PART OF BARANJASIF (Achillea millefolium Linn.) EXTRACTS

Hasah al-Kulya (renal calculi) is one of the leading problems affecting kidney. Recurrence of kidney stone formation is approximately 50%, after 2-3 years of recovery. This makes the prevention of its recurrence an essential problem to address. Despite substantial progress in medical therapy, there is no drug with satisfactory effect. Furthermore, the drugs those are being used for its treatment have many side effects. Baranjasif (Achillea millefolium Linn.) is one of the drugs used in Unani Medicine that has been extensively used for the management of urolithiasis. The aim of this study is to evaluate the lithotriptic action of Baranjasif in-vitro. In this study, calcium oxalate stones were prepared experimentally followed by the preparation of semipermeable membrane by dissolving the outer shell of egg in 2M HCl overnight. Calcium oxalate stones weighing 10mg were then allowed to react with 7 extracts of A. millefolium L. viz, Pet-ether, Chloroform, Ethyl Acetate, Methanol, Acetone, Hydro-alcohol and Aqueous by packing them together in semipermeable membrane. The reaction was performed on three different concentrations of each extract i.e., 50 mg, 100 mg and 150 mg. In addition to 7 groups, two more groups were taken, i) a negative group containing only the stone of weight 10 mg and ii) standard group consisting of stone weighing 10 mg and cystone (in concentrations of 50 mg, 100 mg and 150 mg). Statistical methods like mean standard deviation, percentage dissolution, percentage inhibition, IC and Pearson's correlation were applied to understand the extent of dissolution of stones 50 in each group. The study advocates that Baranjasif reveals lithotriptic activity comparable to standard drug. The efficacy of the drug is directly proportional to its concentration. Ethyl acetate extract of the plant showed the most promising results.

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Exploring the Pharmacognostic Features, Anti-oxidant and Lipid Lowering Potential of Fagopyrum esculentum Moench. Seed

Current Traditional Medicine ◽

10.2174/2215083805666190807105935 ◽

2020 ◽

Vol 6 (2) ◽

pp. 155-169

Author(s):

Neeraj Panihar ◽

Neeru Vasudeva ◽

Sunil Sharma ◽

Babu Lal Jangir

Keyword(s):

Ethyl Acetate ◽

Wistar Rats ◽

Ethanol Extract ◽

Water Soluble ◽

Fagopyrum Esculentum ◽

Lipid Lowering ◽

Standard Drug ◽

Fagopyrum Esculentum Moench ◽

Β Carotene

Background: Fagopyrum esculentum Moench. is a herb consumed as food and has medicinal value. It is a rich source of bioactive nutrients which cure and prevent many ailments. Traditionally, it is used to treat hypertension, diabetes, constipation, cancer etc. Methods and Objective: Present work illustrates morphological, microscopic and physicochemical parameters of Fagopyrum esculentum seeds as per WHO guidelines, in vitro antioxidant activity; assessed by DPPH scavenging method, hydrogen peroxide scavenging assay and β-carotene linoleic acid bleaching method and study of lipid lowering potential of the ethyl acetate and ethanol extract of seeds on normal diet fed Wistar rats. Results: Morphological studies delineated the triangular shape, dark brown colour, 8 mm length and 6 mm width of the seed. The microscopic examination of the transverse section of seed depicted features like testa or pericarp (seed coat), the endosperm, embryo and sclerenchyma cells. Study of physiochemical parameters exhibited 0.3±0.02% of foreign matter and 1.44±0.51% crude fibre content. Total ash, acid insoluble ash and water soluble ash value were 6.7±1.7%, 1.9±0.23% and 3.9± 0.31% respectively. Alcohol soluble and water soluble extractive value came out to be 65.02± 3.21 mg/g and 12.7±1.24 mg/g respectively. Foaming index was less than 100, swelling index was found to be 0.5±0.01 ml/g. Loss on drying was 4.02±1.27%. Phytochemical screening of ethyl acetate and ethanol extract revealed the presence of alkaloids, carbohydrates, phenolic compounds, phytosterols and flavonoids. Trace amount of heavy metals (arsenic, cadmium, lead, mercury) were determined by atomic absorption spectrophotometer. Pesticide residue analysis confirmed the presence of nontoxic pesticides like dimethipin, hymexazol, phenothrin-2, methoprene, triadimenol, prohydrojasmon- 1, jasmolin ii, triademinol, jasmolin i, prohydrojasmone i, cyromazine in both the extracts by gc-ms spectrometer. The ethyl acetate and ethanol extract has shown significant in-vitro antioxidant activities demonstrated by the DPPH method (IC50 = 94.37±2.51 and 216.04±4.39 μg/ml respectively), hydrogen peroxide scavenging assay (IC50 = 83.72±3.72 and 193.47±5.05 µg/ml respectively) and β-carotene bleaching method (IC50 = 100.67±4.01 and 205.39±2.89 µg/ml respectively). Lipid lowering study performed on Wistar rats demonstrated a significant (p<0.001) decrease in serum Total Cholesterol (TC), Triglyceride (TG) and increase in High Density Lipoprotein (HDL) level as compared to normal group. Both the extracts have shown a non significant difference in the level of TG as compared to standard drug atorvastatin, depicting that the efficacy of extracts is at par with that of standard drug atorvastatin. Conclusion: Pharmacognostical study of the plant can be a very good tool for identification as well as authentication of a herb. Moreover, these parameters may be helpful in the development of monograph of the plant. Pharmacological activity confirmed Fagopyrum esculentum Moench. seed to be a good antioxidant and have lipid lowering potential.

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The effect of Gokshura Churna over Tilanalakshara Yoga in the management of Mutrashmari - A Randomized Comparative Clinical Study

Journal of Ayurveda and Integrated Medical Sciences (JAIMS) ◽

10.21760/jaims.v3i5.13811 ◽

2018 ◽

Vol 3 (5) ◽

Author(s):

Dr. Anil M. Sanshi ◽

Dr.Pallavi A. Hegde

Keyword(s):

Abdominal Pain ◽

Renal Calculi ◽

Renal Calculus ◽

P Value ◽

Standard Group ◽

Standard Drug ◽

Female Ratio ◽

Comparative Clinical Study ◽

Group B ◽

Trial Drug

Background: Mutrashmari is a stone like structure anywhere in the Mutravaha Srothas which can be compared with renal calculi and one among the Astamahagada, clinical features as renal angle pain, haematuria, pyuria and dysuria. Peak age is 3rd to 5th decade while majority of patients report regarding onset of disease in 2nd decade of life and male to female ratio is 3:1. Objectives: To compare the effect of Gokshura Churna over Tilanalakshara Yoga in the management of Mutrashmari with special reference to Renal calculi. Materials and Methods: 30 patients were selected on the basis of inclusion criteria and divided into 2 groups with 15 patients in each group by chit method. Group A was treated with standard drug Tilanalakshara Yoga and Group B was traeted with trial drug Gokshura Churna. Results: It was found that standard group reduced the abdominal pain by 85.1%, dysuria by 89.4%, renal angle tenderness 88.4%, haematuria 100%, pyuria 100%, size of renal calculus 41.9% by 21st day of treatment. In study group it was observed that abdominal pain reduced by 92%, dysuria 96.1%, renal angle tenderness 91.6%, haematuria 100%, pyuria 100% and size of renal calculus 48.5% by 21st day of treatment. Conclusion: This assessment showed Gokshura Churna had significant result over Tilanala Kshara Yoga in both subjective and objective parameters with P value less than 0.0001.

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In Vivo Entombment of Bacteria and Fungi during Calcium Oxalate, Brushite and Struvite Urolithiasis

Kidney360 ◽

10.34067/kid.0006942020 ◽

2020 ◽

pp. 10.34067/KID.0006942020

Author(s):

Jessica J. Saw ◽

Mayandi Sivaguru ◽

Elena M. Wilson ◽

Yiran Dong ◽

Robert A. Sanford ◽

...

Keyword(s):

Calcium Oxalate ◽

Stone Formation ◽

Human Kidney ◽

Rrna Gene ◽

Bacterial Cells ◽

Stone Formers ◽

Bacteria And Fungi ◽

Struvite Stones

Background: Human kidney stones form via repeated events of mineral precipitation, partial dissolution and reprecipitation, which are directly analogous to similar processes in other natural and man-made environments where resident microbiomes strongly influence biomineralization. High-resolution microscopy and high-fidelity metagenomic (microscopy-to-omics) analyses, applicable to all forms of biomineralization, have been applied to assemble definitive evidence of in vivo microbiome entombment during urolithiasis. Methods: Stone fragments were collected from a randomly chosen cohort of 20 patients using standard percutaneous nephrolithotomy (PCNL). Fourier transform infrared (FTIR) spectroscopy indicated that 18 of these patients were calcium oxalate (CaOx) stone formers, while one patient each formed brushite and struvite stones. This apportionment is consistent with global stone mineralogy distributions. Stone fragments from 7 of these 20 patients (5 CaOx, 1 brushite and 1 struvite) were thin sectioned and analyzed using brightfield (BF), polarization (POL), confocal, superresolution autofluorescence (SRAF) and Raman techniques. DNA from remaining fragments, grouped according to each of the 20 patients, were analyzed with amplicon sequencing of 16S rRNA gene sequences (V1-V3, V3-V5) and internal transcribed spacer (ITS1, ITS2) regions. Results: Bulk entombed DNA was sequenced from stone fragments in 11 of the 18 CaOx patients, as well as the brushite and struvite patients. These analyses confirmed the presence of an entombed low-diversity community of bacteria and fungi, including Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria, and Aspergillus niger. Bacterial cells ~1 µm in diameter were also optically observed to be entombed and well-preserved in amorphous hydroxyapatite spherules and fans of needle-like crystals of brushite and struvite. Conclusions: These results indicate a microbiome is entombed during in vivo CaOx stone formation. Similar processes are implied for brushite and struvite stones. This evidence lays the groundwork for future in vitro and in vivo experimentation to determine how the microbiome may actively and/or passively influence kidney stone biomineralization.

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A comparative study of the adsorption of amino acids on to calcium minerals found in renal calculi

Clinical Science ◽

10.1042/cs1010159 ◽

2001 ◽

Vol 101 (2) ◽

pp. 159-168 ◽

Cited By ~ 20

Author(s):

David E. FLEMING ◽

Wilhelm VAN BRONSWIJK ◽

Rosemary Lyons RYALL

Keyword(s):

Amino Acids ◽

Calcium Oxalate ◽

Binding Affinity ◽

Stone Formation ◽

Human Kidney ◽

Renal Calculi ◽

Calcium Oxalate Monohydrate ◽

Carboxyglutamic Acid ◽

Calcium Minerals ◽

Ph Range

To assess the binding of individual amino acids to the principal calcium minerals found in human kidney stones, the adsorption of 20 amino acids on to calcium oxalate monohydrate, CaHPO4.2H2O, Ca3(PO4)2 and Ca5(PO4)3OH crystals was determined over the physiological urinary pH range (pH 5–8) in aqueous solutions. All amino acids adsorbed most strongly at pH 5, and this decreased in all cases as the pH was increased. The amino acids which adsorbed most strongly were aspartic acid, glutamic acid and γ-carboxyglutamic acid, with the last displaying the strongest affinity. All amino acids bound more avidly to calcium oxalate monohydrate than to any of the phosphate minerals. Adsorption on to CaHPO4.2H2O was generally higher than for Ca3(PO4)2 and Ca5(PO4)3OH, for which all amino acids, with the exception of γ-carboxyglutamic acid, had only a weak affinity. The binding affinity of these acids is thought to be due to their zwitterions being able to adopt conformations in which two carboxyl groups, and possibly the amino group, can interact with the mineral surface without further rotation. The strong binding affinity of di-and tri-carboxylic acids for calcium stone minerals indicates that proteins rich in these amino acids are more likely to play a functional role in stone pathogenesis than those possessing only a few such residues. These findings, as well as the preferential adsorption of the amino acids for calcium oxalate monohydrate rather than calcium phosphate minerals, have ramifications for research aimed at discovering the true role of proteins in stone formation and for potential application in the design of synthetic peptides for use in stone therapy.

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Chemical Compounds Identification and Antioxidant and Calcium Oxalate Anticrystallization Activities of Punica granatum L.

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/9424510 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14

Author(s):

Rabie Kachkoul ◽

Tarik Squalli Houssaini ◽

Mohamed Mohim ◽

Radouane El Habbani ◽

Anissa Lahrichi

Keyword(s):

Calcium Oxalate ◽

Bioactive Compounds ◽

Biological Activities ◽

Stone Formation ◽

Chemical Species ◽

Punica Granatum ◽

Fruit Peel ◽

Spectrophotometric Methods ◽

Dpph Method

The plant Punica granatum L. has several biological activities and a great curative and preventive power against chronic diseases. For this purpose, the objective of this work is to valorize the fruit peel of this plant in the field of phytomedicine, by quantifying and identifying its bioactive compounds and by evaluating their antioxidant and anticrystallization activities against calcium oxalate. This comparative study has been carried out by hydroalcoholic extract (E.PG) and infusion (I.PG) of the plant. The quantification of the phenolic compounds has been performed by spectrophotometric methods, and the chemical species identification has been performed by UPLC-PDA-ESI-MS. Moreover, the examination of the antioxidant activity has been executed by both methods of DPPH and FRAP. The crystallization inhibition has been studied in vitro by the turbidimetric model. The characterization of the synthesized crystals has been accomplished by microscopic observation and by Fourier Transform Infrared Spectroscopy. The results found show the comparable importance of the two plant extracts in the elimination of free radicals; the values of the half maximal inhibitory concentration “IC50” obtained are in the order of 60.87 ± 0.27 and 59.91 ± 0.83 μg/mL by the DPPH method and in the order of 42.17 ± 7.46 and 79.77 ± 6.91 μg/mL by the FRAP method, for both E.PG and I.PG, respectively. Furthermore, the inhibition percentages of calcium oxalate crystallization are in the range of 98.11 ± 0.17 and 98.22 ± 0.71% against the nucleation and in the order of 88.98 ± 0.98 and 88.78 ± 2.48% against the aggregation, for E.PG and I.PG, respectively. These results prove the richness of the plant in bioactive compounds, offering an antioxidant and anticrystallization capacity; therefore, it can be used in the treatment and/or the prevention of stone formation.

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The Effect of Ethane-1-Hydroxy-1,1-Diphosphonate (EHDP) on Calcium Oxalate Crystalluria in Recurrent Renal Stone-Formers

Clinical Science ◽

10.1042/cs0470013 ◽

1974 ◽

Vol 47 (1) ◽

pp. 13-22 ◽

Cited By ~ 2

Author(s):

W. G. Robertson ◽

M. Peacock ◽

R. W. Marshall ◽

F. Knowles

Keyword(s):

Calcium Oxalate ◽

Stone Formation ◽

Basal Diet ◽

Sodium Oxalate ◽

Calcium Oxalate Crystals ◽

Calcium Oxalate Dihydrate ◽

Calcium Oxalate Stone ◽

Oxalate Crystals ◽

Stone Formers

1. The volume, size and type of calcium oxalate crystals excreted in the urine of a group of patients with recurrent ‘idiopathic’ stones were studied on a controlled basal diet, after an oral supplement of sodium oxalate and after oral administration of ethane-1-hydroxy-1,1-diphosphonate (EHDP) for 4 weeks. 2. Before administration of EHDP the stone-formers passed the large crystals and aggregates of calcium oxalate dihydrate characteristic of recurrent calcium oxalate stone-formers. For the same level of urine saturation and crystalluria EHDP caused a significant reduction in the proportion of large crystals and aggregates excreted. Studies by light-microscopy confirmed that EHDP caused a striking change in the size and habit of calcium oxalate crystals in some but not all of the urine samples examined. 3. The decrease in average crystal size during the administration of EHDP was attributed to the observed increase in the ability of urine to inhibit the growth and aggregation of calcium oxalate crystals as measured by a growth system in vitro. 4. The possible use of EHDP as a therapeutic agent in the treatment of calcium oxalate stone-formation is discussed.

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The Influence of Disodium Ethane-1-Hydroxy-1,1-Diphosphonate (Ehdp) on the Development of Experimentally Induced Urinary Stones in Rats

Clinical Science ◽

10.1042/cs0420197 ◽

1972 ◽

Vol 42 (2) ◽

pp. 197-207 ◽

Cited By ~ 62

Author(s):

D. Fraser ◽

R. G. G. Russell ◽

Ortrun Pohler ◽

W. G. Robertson ◽

H. Fleisch

Keyword(s):

Calcium Oxalate ◽

Stone Formation ◽

Ammonium Phosphate ◽

Urinary Stones ◽

Hydrogen Phosphate ◽

Magnesium Ammonium Phosphate ◽

Magnesium Ammonium ◽

The Difference ◽

Calcium Oxalate Calculi

1. Bladder stones composed of calcium hydrogen phosphate dihydrate, calcium oxalate mono- and di-hydrate and magnesium ammonium phosphate hexahydrate (struvite) were successfully induced in rats by various dietary manipulations and by implanting zinc pellets in the bladder. 2. The effect of a diphosphonate, disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP), given in the drinking water at concentrations of 0·0025, 0·05 and 0·5% (w/v), on the size and composition of these stones was examined. 3. All the concentrations of EHDP decreased the weight of the calcium oxalate calculi. In contrast, only the highest concentration of EHDP inhibited calcium hydrogen phosphate stone formation and the magnesium ammonium phosphate stones were unaffected. 4. The difference between the effects on calcium oxalate and magnesium ammonium phosphate stones is consistent with the finding that EHDP inhibited the precipitation of calcium oxalate from solution in vitro but had only a slight effect on magnesium ammonium phosphate precipitation. 5. It is suggested that EHDP might be of use in the prevention of some types of urinary stones in man.

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Tensile, Flexural and Compressive Strength Studies on Calcium Oxalate Monohydrate Urinary Stone

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.584.494 ◽

2012 ◽

Vol 584 ◽

pp. 494-498

Author(s):

Abdul Rasheed Mohamed Ali ◽

Narayanasamy Arunai Nambi Raj

Keyword(s):

Mechanical Properties ◽

Compressive Strength ◽

Calcium Oxalate ◽

Flexural Modulus ◽

Renal Calculi ◽

Calcium Oxalate Monohydrate ◽

Urinary Stone ◽

Laser Lithotripsy ◽

X Ray Diffraction

Calcium oxalate monohydrate (COM) is the primary constituent of the majority of stones formed in the urinary tract. Mechanical properties of renal calculi dictate how a stone interact and disintegrate with mechanical forces produced by shock wave and laser lithotripsy techniques. Tensile stresses may be more effective in some instances in disrupting material because most materials are weaker in tension than compression. Urinary stone containing COM as a major component was subjected to tensile, flexural and compressive strength studies in order to understand its mechanical properties in vitro. The calculated tensile breaking strength for the urinary stone from three tests varies from 0.57 MNm-2 to 1.52 MNm-2. The flexural strength and the flexural modulus of the urinary stone were calculated as 5.17 MNm-2 and 2.22 GNm-2 respectively while the observed compressive strength was 6.11 MNm-2. The chemical composition and the crystalline nature of the stone were verified using Fourier Transform Infrared spectroscopy and X-ray diffraction.

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