scholarly journals PHYTOCHEMICAL SCREENING AND PRELIMINARY ASSESSMENT OF ANTIBACTERIAL ACTIVITY OF LEAVES AND STEM BARK EXTRACTS OF Dasyphyllum tomentosum (Spreng.) Cabrera

2013 ◽  
Vol 14 (1) ◽  
Author(s):  
Cristiane Da Silva Paula ◽  
Maria Christina dos Santos Verdam ◽  
Angela Maria De Souza ◽  
Beatriz Cristina Konopatzki Hirota ◽  
Obdúlio Gomes Miguel ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Stem Bark ◽  
Phytochemical Screening ◽  
Preliminary Assessment ◽  
De Se

Dasyphyllum tomentosum (Spreng) Cabrera é conhecida popularmente por açucará, agulheira, espinho-de-agulha e goiapá, pertence à família Asteraceae cujos representantes destacam-se principalmente pelas propriedades terapêuticas. O objetivo deste estudo foi uma investigação qualitativa do perfil químico da planta  a fim de se identificar as principais classes de constituintes presentes nas folhas e casca do caule, além da avaliação preliminar da atividade antibacteriana, tendo em vista a inexistência de estudos na espécie. Para a análise fitoquímica o extrato hidro alcoólico 20% e extrato aquoso foram submetidos reações clássicas de caracterização dos grupamentos químicos, e para avaliação da atividade antibacteriana, o extrato etanólico bruto foi submetido ao ensaio de determinação da Concentração Inibitória Mínima.  A análise fitoquímica apontou a presença de triterpenos e/ou esteroides, flavonoides, alcaloides, heterosídeos antociânicos e taninos. Os extratos etanólicos das folhas e casca do caule de Dasyphyllum tomentosum mostraram ausência de atividade inibitória frente aos microorganismos testados.

scholarly journals Phytochemical Screening and Antibacterial Activity of Stem Bark, Leaf and Root Extract of Sclerocarya birrea (A. Rich.) Hochst

2017 ◽  
Vol 5 (4) ◽  
pp. 127-131
Author(s):  
Lawaly Maman Manzo ◽  
Halima Diallo Bako ◽  
Moussa Idrissa
Keyword(s):  
Antibacterial Activity ◽  
Gastrointestinal Diseases ◽  
Stem Bark ◽  
Root Extract ◽  
Root Bark ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Traditional Use ◽  
Plant Parts ◽  
Sclerocarya Birrea

Background: Sclerocarya birrea is widely used in Nigerien communities for medicinal purposes to treat several gastrointestinal diseases including diarrhea. To lend credibility to its traditional use against diarrhea, laboratory studies were conducted. Objective: The aim of this study is to investigate the antibacterial activity and the phytochemical constituents of the crude extracts of root, bark and leaf of S. birrea. Materials and Methods: The collected different plant parts were air dried, powdered and separately extracted with ethanol and methanol. The alkaloid, flavonoid, saponin and tannin contents in all the plant parts were estimated using standard methods. The total and serially diluted fractions of the extracts were tested for antibacterial activity against selected enteropathogens by agar well diffusion and deep-well microdilution method. Results: Phytochemical screening revealed the presence of flavonoid, saponin and tannin in all the plant extracts. The extracts from the different parts showed varied antibacterial activity against the test bacteria. The bark extracts showed superior activity against Escherichia coli (zone of inhibition = 17 mm) and Salmonella typhi (zone of inhibition = 20 mm) at 200 mg/mL. Conclusion: The presence of important phytochemical groups and the antibacterial potential of alcoholic extracts of S. birrea could permit to justify its traditional usage against diarrhea.

scholarly journals Phytochemical, Antioxidant and Antimicrobial studies of Lannea egregia Engl. & K. Krause (Anacardiaceae) extracts and chromatographic fractions

2020 ◽  
Vol 19 (1) ◽  
pp. 348-363
Author(s):  
Philip A. Idowu ◽  
Loveth C. Ekemezie ◽  
Charles O. Olaiya
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Reducing Power ◽  
Stem Bark ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Crude Extracts ◽  
Different Parts

Screening ‘new’ medicinal plants of traditional importance for bioactive components is a sure way of discovering novel therapeutic agents to treat diseases. This study, therefore investigated the presence of phytochemical, antioxidant and antibacterial components of the extracts of Lannea egregia. Phytochemical screening was done by standard methods. Antibacterial activity of the extracts of Lannea egregia was determined by agar well diffusion method while the minimum inhibitory concentration (MIC) was determined by agar dilution method. The antioxidant capacity of the crude extracts was determined through the evaluation of total flavonoid content, total phenolic content, ferric reducing power, total antioxidant capacity and 2,2-diphenyl-1-picryhydrazyl. The phytochemical screening of the different parts of this plant revealed the presence of tannins, saponins, alkaloids, flavonoids, steroids, terpenoids, emodins, phlobatannins, anthocyanins, coumarins and phenolics. Phlobatannins was observed to be absent in the stem bark. The crude extracts obtained from the leaves, stem bark and roots of this plant exhibited good antibacterial activity against typed strains of Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. The diameter of the zone of inhibition ranged from 9.0 to 26.0 mm at 100 mg/mL for all the plant parts. The ethyl acetate leaf extract of this plant possessed the highest antibacterial activity with MIC and MBC values of the range of (3.125 to ˃50 mg/mL) and (12.5 to ˃50 mg/mL) respectively. The zone inhibition of the chromatographic fractions of both plants ranged 15-23 mm. Antioxidant study of the extracts of the leaf of L. egregia revealed that the ethyl acetate and methanol extracts have good antioxidant potentials comparable to that of ascorbic acid control. This study has revealed that the extracts from different parts of L. egregia possess good antibacterial and antioxidant activities which could be a function of the various phytochemicals detected in the plant. Keywords: Lannea egregia, Phytochemical, Antibacterial, Antioxidant, Column chromatography.

scholarly journals Phytochemical Screening and Antibacterial Activity of Leaf and Stem Bark Extracts of Adansonia digitata on E. coli, S. aureus and S. typhi

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Mohammed Bashir ◽  
Auwal Ibrahim ◽  
Mohammed Bilyaminu ◽  
Rufai Ali ◽  
Halima Isa ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Stem Bark ◽  
Phytochemical Screening ◽  
Adansonia Digitata ◽  
E Coli

scholarly journals Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Babe Guyasa ◽  
Yadessa Melaku ◽  
Milkyas Endale
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Fungal Infections ◽  
2D Nmr ◽  
Stem Bark ◽  
Spectroscopic Techniques ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
E Coli

Embelia schemperi Vatke is one of the medicinal plants used traditionally for treatment of intestinal tape worm, dysmenorrheal, bacterial, and fungal infections. Phytochemical screening test of the dichloromethane/methanol (1 : 1) and methanol extracts revealed the presence of phenols, alkaloids, tannins, and flavonoids whereas terpenoids, glycoside, and phytosterols were absent. Silica gel column chromatographic separation of the methanol extract afforded 3,5,7,3′,4′-pentahydroxyflavan, named epicatechin (1), along with a close flavan derivative (2). Structures of the compounds were elucidated by spectroscopic techniques (1D and 2D NMR, FTIR, and UV-Vis). The crude extracts and isolated compounds were screened for in vitro antibacterial activity against strains of Klebsiella pneumoniae, Escherichia coli, Cryptococcus neoformans, Shigella dysentriae, and Staphylococcus aureus. Epicatechin (1) exhibited comparable antibacterial activity against S. aureus and E. coli (15 and 12 mm zone of inhibition, resp.) to that of the control antibiotic gentamicin, with zone of inhibition of 15 and 12 mm, respectively, at a concentration of 20 µg/mL.

scholarly journals Phytochemistry and Preliminary Assessment of the Antibacterial Activity of Chloroform Extract ofAmburana cearensis(Allemão) A.C. Sm. againstKlebsiella pneumoniaeCarbapenemase-Producing Strains

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Mirivaldo Barros Sá ◽  
Maria Taciana Ralph ◽  
Danielle Cristina Oliveira Nascimento ◽  
Clécio Souza Ramos ◽  
Isvânia Maria Serafin Barbosa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Hydroxybenzoic Acid ◽  
Preliminary Assessment ◽  
Good Source

The chloroform extract of the stem bark ofAmburana cearensiswas chemically characterized and tested for antibacterial activity.The extract was analyzed by gas chromatography and mass spectrometry. The main compounds identified were 4-methoxy-3-methylphenol (76.7%), triciclene (3.9%),α-pinene (1.0%),β-pinene (2.2%), and 4-hydroxybenzoic acid (3.1%). Preliminary antibacterial tests were carried out against species of distinct morphophysiological characteristics:Escherichia coli,Salmonella entericaSerotype Typhimurium,Pseudomonas aeruginosa,Staphylococcus aureus,Listeria monocytogenes, andBacillus cereus. The minimum inhibitory concentration (MIC) was determinate in 96-well microplates for the chloroform extract and an analogue of themain compound identified, which was purchased commercially.We have shown that plant’s extract was only inhibitory (but not bactericidal) at the maximum concentration of 6900 μg/mL againstPseudomonas aeruginosaandBacillus cereus. Conversely, the analogue 2-methoxy-4-methylphenol produced MICs ranging from215 to 431 μg/mL against all bacterial species.New antibacterial assays conducted with such chemical compound againstKlebsiella pneumoniaecarbapenemase-producing strains have shown similarMICresults and minimumbactericidal concentration (MBC) of 431 μg/mL.We conclude thatA. cearensisis a good source of methoxy-methylphenol compounds,which could be screened for antibacterial activity againstmultiresistant bacteria fromdifferent species

Sign in / Sign up

Export Citation Format

Share Document