scholarly journals QUALIDADE MICROBIOLÓGICA DE LINGÜIÇAS MISTAS DO TIPO FRESCAL PRODUZIDAS NA CIDADE DE PELOTAS (RS)

Author(s):  
WLADIMIR PADILHA DA SILVA ◽  
ELIEZER AVILA GANDRA ◽  
EDUARDA HALLAL DUVAL ◽  
MÁRCIA MONKS JANTZEN ◽  
CRISTIANE TESSMANN ◽  
...  

Foram avaliadas as condições higiênico-sanitárias de lingüiças mistas do tipo frescal, provenientes de 4 frigoríficos submetidos à inspeção sanitária estadual, e comercializadas em Pelotas (RS). Os resultados obtidos foram interpretados pela legislação vigente (RDC no 12/01) e pela anterior (Portaria 451), ambas do Ministério da Saúde (Brasil). Verificou-se que 17,86% das 32 amostras estavam contaminadas com Salmonella sp e 3,57% apresentaram Coliformes fecais acima dos parâmetros permitidos pela legislação em vigor. Com a alteração da legislação, a probabilidade de se encontrar amostras de lingüiça mista frescal contaminada por Coliformes fecais passou de 23,27% para 5,6%. MICROBIOLOGICAL QUALITY OF MIXING SAUSAGES OF THE FRESCAL TYPE PRODUCED IN PELOTAS - RS Abstract The hygienic-sanitary conditions of mixing sausages of the frescal type were evaluated, proceeding from 4 slaughterhouses with state sanitary inspection and commercialized in Pelotas (RS). The results were interpreted by the current legislation or the previous one, both of the Brazilian Health Ministry, in the interpretation of the results. It was verified that, 17,86% of the 32 samples were contaminated with Salmonella sp and 3.57% presented faecal coliforms above of the parameter allowed by the current legislation. With the change of the legislation, the probability to find samples of frescal mixing sausage with countings of faecal coliforms above of the maximum limits allowed by the legislation, passed of 23,27% to 5,6%.

2009 ◽  
Vol 39 (6) ◽  
pp. 1836-1841 ◽  
Author(s):  
Thaís Mioto Martineli ◽  
Oswaldo Durival Rossi Junior ◽  
Natacha Deboni Cereser ◽  
Marita Vedovelli Cardozo ◽  
Cristianne Lino Fontoura ◽  
...  

The consumption of lamb meat in Brazil has increased in the last years but little information about the microbiological quality of this product is available. To evaluate the hygienic-sanitary conditions of lamb carcasses, the quantification of microorganism populations indicators (mesophiles and psychrotrophs; total and thermotolerant coliforms; Escherichia coli; moulds and yeasts) and the pathogenic microorganisms indentification (Salmonella sp. and Listeria spp.) were performed. A total of 60 lamb carcasses were sampled from one abattoir in São Paulo. Swab samples were collected from three points (forequarter, back and hindquarter) on the muscle surface after carcasses final washing. Statistical analysis consisted of descriptive evaluation of the results whose counts were grouped by intervals of microorganism populations. Counts ranged from 1.0 x 10¹ to 8.0 x 10(4) colony-forming unit cm-2 (CFU cm-2) for mesophiles; 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for psychrotrophs; < 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for moulds and yeasts; < 0.3 to > 32.0 most probable number/cm² (MPN cm-2) for total and thermotolerant coliforms and Escherichia coli. Salmonella sp. and Listeria spp. were not found in any of the carcasses. Most carcasses presented low counts for all microorganisms. Overall results may be explained by the small size of the industry where the study was taken. Results suggest that good microbiological quality lamb meat is possible to be obtained, but improvement in hygienic-sanitary conditions is still required.


2020 ◽  
Vol 8 (2) ◽  
pp. 14
Author(s):  
KPATA-KONAN Nazo Edith ◽  
YAO N’Zué Benjamin ◽  
COULIBALY Kalpy Julien ◽  
KONATÉ Ibrahim

This article looked at the quantity and storage time of attiéké produced and sold in the town of Daloa. It also examines the microbiological characteristics of attiéké-femme and attiéké-garba from this locality during storage. The study was carried out on the one hand through a field survey carried out on the producers and sellers. On the other hand, a sample was taken from 10 sellers of attiéké-garba and 10 sellers of attiéké-woman. The study found that the women producers sell 87% of their production in the city of Daloa and export 13%. In addition, attiéké can be kept for 2 days at the producers and beyond 2 days at the sellers before their stock runs out. Therefore, a weekly production of more than 200 kg for the majority of the producers is observed. Microbiological analyses showed high levels of germs (MAG: 6.106 CFU/g; Yeasts and moulds: 2.7.106 CFU/g) for attiéké-women and (2.106 CFU/g of GAM and 1.6.103 CFU/g of Yeasts and moulds) for attiéké-garba. Total coliforms and faecal coliforms were only found in attiéké-women. No salmonella was observed. In view of the results, it should be noted that female attiéké is the most contaminated type of attiéké.


1980 ◽  
Vol 43 (5) ◽  
pp. 385-389 ◽  
Author(s):  
C. JANE WYATT ◽  
V. GUY

A sanitation profile scoring form for evaluating sanitation in retail food stores was designed. The profile was tested in 10 Oregon retail markets to evaluate its ability to reflect sanitary conditions. At the time of inspection, samples of meat processed in-store were purchased for microbiological analysis to explore the feasibility of bacterial quality as a measurement of sanitary conditions. Microbiological tests performed included total aerobic plate count (A PC), coliform, Escherichia coli, Staphylococcus aureus, Clostridium perfringens, and Salmonella. Certain deficiencies were noted in the profile designed; however, it provides a means for objective, uniform measurement of sanitary conditions. Data show no correlation exists between microbiological quality of products processed in the store and total store profile sanitary conditions. Fifty percent of the products sampled exceeded bacterial load guidelines currently enforced in Oregon. These “high” counts appear to be directly related to poor temperature control.


2013 ◽  
Vol 11 (2) ◽  
Author(s):  
Septia Anggraini ◽  
Erna Triastuti ◽  
Moh. Muchson

Intensive nursing rooms is known high risk zone in which the quality of air should bekept up to the highest standard because it may result in various health disorders. Thisresearch aims to discover the quality of air within the intensive nursing rooms of the BrawijayaHospital in Surabaya in 2013 through comparison with designated standards set forth inhealth ministry decree No. 1204/MENKES/SK/X/2004 re: health requirements of for hospitals.This descriptive research has been conducted at Brawijaya Hospital Surabaya in May2013. There were 11 rooms under study to be represented by air samples taken from 3rooms, i.e. Nusa Indah Room, Dahlia Room, and Tulip Room. The variables to be studiedamong others were microbiological quality of air as shown by number of germs, physicalmeasurements consisting temperature and humidity, assessment of room construction, densityof occupation, and purifying process.Results indicated that the highest number of germ before purifying process was 444CFU/m3 while at the time of patient visitation the number was 672 CFU/m3 meaning that theydid not comply to the requirements stated in health ministry decree no.1204/MENKES/SK/X/2004 which stated that the number of germs in children nursing room is200 CFU/m3 and adult nursing room is 200-500 CFU/m3• The highest temperature recorded atthe time before purifying process was 30°C and the humidity was 57%; while at the time ofpatient visitation the measurements indicated a temperature of 32°C and humidity of 58%.Density assessment has complied to the designated requirements: for a children nursing roomis 2 m2/bed and adult nursing room is 4.5 m2/bed. Pertaining to room and buildingconstruction of the intensive nursing ward that did not comply to the requirement was 60%while the purifying proces which did not comply with the requirements was 54%.Research concluded that number of germs in Nusa Indah Room at the time beforepurifying process and in Tulip Room at the time of patient visitation were clearly not complyingto the requirements. The suggestion included monitoring the quality of air on a regular basisin accordance with the health minister decree No. 1204/MENKES/SK/X/2004.Keyword: Mikrobiological, Air Quality, Intensif Nursing Wards


Author(s):  
MARIA DE FÁTIMA BORGES ◽  
TEREZINHA FEITOSA ◽  
RENATA TIEKO NASSU ◽  
CELLI RODRIGUES MUNIZ ◽  
ÉRIKA HARDY FRANCO DE AZEVEDO ◽  
...  

Avaliou-se a qualidade higiênico-sanitária de 43 amostras de queijo de coalho produzidas em diferentes microrregiões do estado do Ceará. Bolores e leveduras foram detectados em 100% das amostras de queijos, com contagem variando de 1,7 x 104 a 1,6 x 109 UFC/g. Todas as amostras apresentaram coliformes totais e fecais, com confirmação de Escherichia coli em 93,1% das mesmas. Foi verificada a presença de Estafilococos coagulase positiva em 93,1% das amostras de queijos, com contagens variando de 1,0 x 101 a 2,0 x 109 UFC/g. Apenas 2,3% das amostras encontravam-se de acordo com os padrões microbiológicos vigentes para essa bactéria. A presença de Salmonella foi constatada em 34,9% das amostras de queijos. Listeria sp . foi detectada em 6,9% das amostras, com confirmação de L. monocytogenes em 2,3% dessas. A elevada população de bolores e leveduras (observada em 100% das amostras de queijos de coalho) indicou deficiência nos procedimentos de higiene e sanitização das amostras, caracterizando-as como produto em condições higiênicas insatisfatórias. A alta concentração de estafilococos coagulase positiva e de coliformes fecais (aproximadamente 90% das amostras) caracterizou os queijos como produto em condições higiênico-sanitárias insatisfatórias. Queijos de coalho oriundos das cinco microrregiões do Ceará envolvidas no estudo não apresentaram segurança alimentar, visto que a maioria continha estafilococos coagulase positiva, L. monocytogenes e Salmonella. A presença dessas duas últimas bactérias permitiu classificar os queijos como produtos impróprios para consumo humano. PATHOGENIC AND INDICATOR MICROORGANISMS ISOLATED FROM “COALHO”CHEESE PRODUCED IN THE CEARÁ STATE, BRAZIL Abstract The hygienic-sanitary quality of 43 “coalho” cheese samples produced in different regions of the Ceará state was evaluated. Yeasts and molds were detected in 100% of the samples, varying from 1.7 x 104 to 1.6 x 109 CFU/g. All of the samples presented total and faecal coliforms with confirmation of Escherichia coli in 93.1% of the samples. Positive coagulase staphylococci were observed in 93,1% of the samples with countings ranging from 1.0 x 101 to 2.0 x 109 CFU/g. Only 2.3% of the samples were in accordance with the actual microbiological patterns for this bacteria. Presence of Salmonella was confirmed in 34.9% of the cheese samples. Listeria sp . was detected in 6.9% of the samples and posterior confirmation of L. monocytogenes in 2.3% of the samples. The elevated population of yeasts and molds (observed in 100% of the samples) indicated deficiency in the hygiene and sanitation proceedings becoming characterized as “product at unsatisfactory hygienic conditions”. The high population of positive coagulase staphylococci characterized the cheeses as “product at unsatisfactory hygienic and sanitary conditions”. “Coalho” cheeses originated from five regions of Ceará state involved in this study did not present food safety, mainly because of the presence of positive coagulase staphylococci, L. monocytogenes and Salmonella. The presence of the two last bacteria permitted to classify the cheeses as inappropriate to the human consumption.


2018 ◽  
Vol 10 (2) ◽  
pp. 178
Author(s):  
Maíra Pessoa Jornane Barbosa Santos ◽  
Jorge Raimundo Lins Ribas ◽  
Carlos Eduardo Crispim de Oliveira Ramos ◽  
Rodrigo Dianna Navarro ◽  
Denise Soledade Peixoto Pereira ◽  
...  

Oyster farming in Brazil is limited by a number of problems, such as lack of sanitary control in production areas. The aim of the present study was to use the multiple correspondence analyses (MCA) to correlate the microbiological quality of oyster production in the state of Bahia, Brazil, by linking it to environment parameters. Samples were randomly collected from 15 different oyster farms, from two different cultivation techniques (lantern and pillow baskets), corresponding dry and rainy periods. Twenty eight samples were collected, stored at 5 °C and send to the laboratory. The analyzed environmental parameters were pH, temperature, dissolved oxygen, salinity, and polluting sources like sewage outflow. Coliforms were counted at 35 °C and 45 °C: Escherichia coli, Salmonella sp., Pseudomonas sp. and Aeromonas sp. Statistical analyzes were performed using the data sizing reduction procedure (MCA), through the SPPS Program, version 18.0. A positive relationship was found between the dry period and lantern net farming with poor sanitary conditions. MCA allowed the analyzed commercial oyster farms to form three distinct groups, helped define the intervention strategies of these commercial oyster farms and provided the basis for implementing health protection measures.


1997 ◽  
Vol 35 (11-12) ◽  
pp. 147-150 ◽  
Author(s):  
B. Mendes ◽  
P. Urbano ◽  
C. Alves ◽  
N. Lapa ◽  
J. Morais ◽  
...  

An evaluation of the sanitary quality of the sand beaches of Azores archipelago was undertaken May-October 1994 and 1995. The samples were analysed for total and faecal coliforms, faecal enterococci, Clostridium, Pseudomonas aeruginosa and fungi (keratinolytic, yeasts, potential pathogenic and allergic and/or environmental saprophytes). The results were evaluated according to a “Proposal Guideline for the Microbiological Quality of Sand”. The data showed good or satisfactory sanitary quality of the sand beaches. The results indicated that the faecal enterococci can be a better indicator organism than the total and faecal coliforms. The fungi could be used as indicator organisms. The application of the “Proposal” should complete the criteria used in the “Blue Flag” distinction for high environmental quality beaches.


2019 ◽  
Vol 1 ◽  
pp. 12 ◽  
Author(s):  
Lydia Mosi ◽  
Samuel Mawuli Adadey ◽  
Sandra Akoley Sowah ◽  
Charles Yeboah

Background: Sachet water, popularly known as “pure water” has become an invaluable entity in most Ghanaian households. Despite its importance, there is no extensive nationwide investigations on its wholesomeness for consumption. The aim of this study was to determine the microbiological quality of 41 brands of sachet water sampled in 16 districts across 5 regions in Ghana. Methods: The samples were analyzed for the presence of total and fecal coliform (Escherichia coli) using the Colilert*- 18 Test Kit. Results: Majority of the samples (56.09%) were excellent, 4.87% satisfactory and 14.63% suspicious. Ten samples (24.4%) were unsatisfactory. For the degree of fecal contamination, (85.56%) were satisfactory, four (9.76%) were suspicious, and two others (4.88%) were unsatisfactory. The contaminations observed could be attributed to poor sanitary conditions (during and/or after production) and failure of some production facilities to adhere to standard manufacturing practices. Conclusion: Our data suggest that microbiological quality sachet water from some sources have not yet attained levels that make it absolutely pure and wholesome for consumption in many areas.


1997 ◽  
Vol 35 (11-12) ◽  
pp. 205-209 ◽  
Author(s):  
L. Botero ◽  
M. Montiel ◽  
P. Estrada ◽  
M. Villalobos ◽  
L. Herrera

Waste stabilisation ponds are an efficient means of wastewater treatment in many parts of the world wherever suitable land is available at reasonable cost and solar energy is an abundant energy resource. This study evaluated the removal of total coliforms TC, faecal coliforms FC and coliphages C in waste stabilisation ponds functioning as a pilot system in the tropical climate of Maracaibo, Venezuela. Sampling points included raw sewage and each pond effluent. Turbidity, pH and temperature were recorded. The results for raw sewage show average levels of 4.1×106 TC, 2.8×106 FC and 7.0×105 C/100mL. Temperature, pH and turbidity ranges between 26–31°C, 6.2–9.5 and 15–98 NTU respectively. Removal of microorganisms in the three systems ranged between 93–98%. Despite the high removal efficiency of microorganisms, the final effluents showed average counts of 5.4×104−1.4×105 TC, 5.2×104−1.3×105 FC and 1.6×104−4.7×104 C/100mL. This study shows that the microbiological quality of the final effluents did not achieve the WHO water quality requirement for FC (103/100mL); therefore, they cannot be used for irrigation. Additional treatments, such as slow sand filtration, are needed in order to improve the quality of the water.


2018 ◽  
Vol 84 (0) ◽  
Author(s):  
Lucélia Guimarães ◽  
Ana Clara dos Santos ◽  
Elka Ferreira ◽  
Débora Pereira ◽  
Francisca Costa

ABSTRACT: Fish are considered rich sources of nutrients. Health care throughout its production chain aims to ensure quality, minimizing the risks of transmission of foodborne diseases. In order to evaluate the microbiological quality of trahira fish (H. malabaricus), 40 samples were analysed for Most Probable Number (MPN) of coliforms at 45ºC, counts of aerobic mesophilic bacteria and Staphylococcus spp., identification of E. coli, Salmonella spp. and Aeromonas spp.. Analyses were conducted according to official methods, procedures, and recommendations. Microbiological results showed coliform values at 45 °C ranging from <3 to > 1.1 × 103 MPN/g, presence of E. coli in 14 (35%) samples, counts of mesophilic aerobic bacteria from 9 × 102 to 109 CFU/g and absence of coagulase positive Staphylococcus aureus. Salmonella spp. was detected in 2 (5%) samples, which is in disagreement with the standards required by the RDC Nº 12 of January, 2001 (ANVISA) regarding Aeromonas spp. In total, 36 (90%) samples were contaminated, 7 (19.4%) by A. cavie and 29 (80.6%) by A. hidrophila. The results of this research showed unsatisfactory hygienic and sanitary conditions of fish from the municipality of São Bento (MA), exposing consumers to the risk of foodborne diseases.


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