scholarly journals Effects of Waste Disposal: Temporo-Spatial Patterns of Bacterial Community Composition on Oyster Farms in Estuary Regions of Bahia, Brazil

2018 ◽  
Vol 10 (2) ◽  
pp. 178
Author(s):  
Maíra Pessoa Jornane Barbosa Santos ◽  
Jorge Raimundo Lins Ribas ◽  
Carlos Eduardo Crispim de Oliveira Ramos ◽  
Rodrigo Dianna Navarro ◽  
Denise Soledade Peixoto Pereira ◽  
...  

Oyster farming in Brazil is limited by a number of problems, such as lack of sanitary control in production areas. The aim of the present study was to use the multiple correspondence analyses (MCA) to correlate the microbiological quality of oyster production in the state of Bahia, Brazil, by linking it to environment parameters. Samples were randomly collected from 15 different oyster farms, from two different cultivation techniques (lantern and pillow baskets), corresponding dry and rainy periods. Twenty eight samples were collected, stored at 5 °C and send to the laboratory. The analyzed environmental parameters were pH, temperature, dissolved oxygen, salinity, and polluting sources like sewage outflow. Coliforms were counted at 35 °C and 45 °C: Escherichia coli, Salmonella sp., Pseudomonas sp. and Aeromonas sp. Statistical analyzes were performed using the data sizing reduction procedure (MCA), through the SPPS Program, version 18.0. A positive relationship was found between the dry period and lantern net farming with poor sanitary conditions. MCA allowed the analyzed commercial oyster farms to form three distinct groups, helped define the intervention strategies of these commercial oyster farms and provided the basis for implementing health protection measures.

2009 ◽  
Vol 39 (6) ◽  
pp. 1836-1841 ◽  
Author(s):  
Thaís Mioto Martineli ◽  
Oswaldo Durival Rossi Junior ◽  
Natacha Deboni Cereser ◽  
Marita Vedovelli Cardozo ◽  
Cristianne Lino Fontoura ◽  
...  

The consumption of lamb meat in Brazil has increased in the last years but little information about the microbiological quality of this product is available. To evaluate the hygienic-sanitary conditions of lamb carcasses, the quantification of microorganism populations indicators (mesophiles and psychrotrophs; total and thermotolerant coliforms; Escherichia coli; moulds and yeasts) and the pathogenic microorganisms indentification (Salmonella sp. and Listeria spp.) were performed. A total of 60 lamb carcasses were sampled from one abattoir in São Paulo. Swab samples were collected from three points (forequarter, back and hindquarter) on the muscle surface after carcasses final washing. Statistical analysis consisted of descriptive evaluation of the results whose counts were grouped by intervals of microorganism populations. Counts ranged from 1.0 x 10¹ to 8.0 x 10(4) colony-forming unit cm-2 (CFU cm-2) for mesophiles; 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for psychrotrophs; < 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for moulds and yeasts; < 0.3 to > 32.0 most probable number/cm² (MPN cm-2) for total and thermotolerant coliforms and Escherichia coli. Salmonella sp. and Listeria spp. were not found in any of the carcasses. Most carcasses presented low counts for all microorganisms. Overall results may be explained by the small size of the industry where the study was taken. Results suggest that good microbiological quality lamb meat is possible to be obtained, but improvement in hygienic-sanitary conditions is still required.


1980 ◽  
Vol 43 (5) ◽  
pp. 385-389 ◽  
Author(s):  
C. JANE WYATT ◽  
V. GUY

A sanitation profile scoring form for evaluating sanitation in retail food stores was designed. The profile was tested in 10 Oregon retail markets to evaluate its ability to reflect sanitary conditions. At the time of inspection, samples of meat processed in-store were purchased for microbiological analysis to explore the feasibility of bacterial quality as a measurement of sanitary conditions. Microbiological tests performed included total aerobic plate count (A PC), coliform, Escherichia coli, Staphylococcus aureus, Clostridium perfringens, and Salmonella. Certain deficiencies were noted in the profile designed; however, it provides a means for objective, uniform measurement of sanitary conditions. Data show no correlation exists between microbiological quality of products processed in the store and total store profile sanitary conditions. Fifty percent of the products sampled exceeded bacterial load guidelines currently enforced in Oregon. These “high” counts appear to be directly related to poor temperature control.


2019 ◽  
Vol 10 (4) ◽  
pp. 382-390
Author(s):  
A. A. Antoshyn ◽  
V. I. Nikitin

The existing methods for monitoring the performance of multi-criteria fire detectors do not provide for verification of their characteristics in the conditions of transition from smoldering to flame burning. The aim of the work is the development of the research methods of the environmental parameters during the transition from smoldering (pyrolysis) to flame combustion for simulation a test fire while checking the quality of multi-criteria fire detectors.A technique to conduct research of environmental parameters under conditions of heating wood samples of different sizes to a temperature of selfignition and burning crumpled and smooth paper has been developed.Changes in the concentration of carbon monoxide, specific optical density, and scattering ability during the transition from smoldering (pyrolysis) to flame burning of prepared wood and crumpled paper were studied for the first time.It is shown that the controlled environmental parameters during the transition from smoldering pyrolysis to flame burning change together. Conclusion: the speed of growth of the scattering ability of smoke decreases by 2.4 times, the speed of increase in the specific optical density and concentration of carbon monoxide increases by 2 and 5.3 times (respec-tively), during the transition from pyrolysis to flame burning of wood.


Author(s):  
WLADIMIR PADILHA DA SILVA ◽  
ELIEZER AVILA GANDRA ◽  
EDUARDA HALLAL DUVAL ◽  
MÁRCIA MONKS JANTZEN ◽  
CRISTIANE TESSMANN ◽  
...  

Foram avaliadas as condições higiênico-sanitárias de lingüiças mistas do tipo frescal, provenientes de 4 frigoríficos submetidos à inspeção sanitária estadual, e comercializadas em Pelotas (RS). Os resultados obtidos foram interpretados pela legislação vigente (RDC no 12/01) e pela anterior (Portaria 451), ambas do Ministério da Saúde (Brasil). Verificou-se que 17,86% das 32 amostras estavam contaminadas com Salmonella sp e 3,57% apresentaram Coliformes fecais acima dos parâmetros permitidos pela legislação em vigor. Com a alteração da legislação, a probabilidade de se encontrar amostras de lingüiça mista frescal contaminada por Coliformes fecais passou de 23,27% para 5,6%. MICROBIOLOGICAL QUALITY OF MIXING SAUSAGES OF THE FRESCAL TYPE PRODUCED IN PELOTAS - RS Abstract The hygienic-sanitary conditions of mixing sausages of the frescal type were evaluated, proceeding from 4 slaughterhouses with state sanitary inspection and commercialized in Pelotas (RS). The results were interpreted by the current legislation or the previous one, both of the Brazilian Health Ministry, in the interpretation of the results. It was verified that, 17,86% of the 32 samples were contaminated with Salmonella sp and 3.57% presented faecal coliforms above of the parameter allowed by the current legislation. With the change of the legislation, the probability to find samples of frescal mixing sausage with countings of faecal coliforms above of the maximum limits allowed by the legislation, passed of 23,27% to 5,6%.


2017 ◽  
Vol 80 (9) ◽  
pp. 1429-1435 ◽  
Author(s):  
Giulia Tabanelli ◽  
Chiara Montanari ◽  
Aldo Gardini ◽  
Mirko Maffei ◽  
Chiara Prioli ◽  
...  

ABSTRACT The aim of this work was to evaluate the microbiological quality of striped venus clams (Chamelea gallina L.) harvested in the north Adriatic Sea during an 8-year monitoring period. A total of 387 samples were analyzed to assess the presence of Escherichia coli. Environmental parameters (salinity, pH, dissolved oxygen, seawater temperature, and freshwater outflow) were collected to find out a possible relationship between the E. coli counts and environmental factors. The results evidenced that the microbiological quality of the clams was good, with only about 5% of the samples not complying with European and Italian regulations for this product, that is, with E. coli counts higher than 230 most probable number (MPN) per 100 g of flesh and intravalvar liquid. Statistical analyses revealed a relationship between microbial contamination and the season and water temperature, probably due to the difference in the filtering activity of the mollusks. However, the main factor affecting the E. coli concentration in the clams turned out to be the flow rate of Marecchia, the major river that reaches the sea in the area of harvesting. In fact, a model fitted to evaluate the probability of finding a higher E. coli count in relation to the environmental parameters evidenced that it was an increase of the level of the Marecchia led to a higher probability of elevated E. coli contamination. This result could be explained by the higher supply of both nutrients and coliforms (including E. coli) when the river is higher and by the anthropogenic characteristics of the lands crossed by the river.


2005 ◽  
Vol 3 (4) ◽  
pp. 371-380 ◽  
Author(s):  
A. C. Vantarakis ◽  
A. Tsibouxi ◽  
D. Venieri ◽  
G. Komninou ◽  
M. Papapetropoulou ◽  
...  

To evaluate the microbiological water quality of bathing sites along the Achaia coastline (south western Greece), a survey was conducted to determine the concentration of faecal bacterial and phage indicators as well as the presence of human viruses. Seawater samples (234) were collected from nine bathing sites on the Achaia coastline and were analysed for the presence of: total coliforms, faecal coliforms, faecal streptococci, Escherichia coli, somatic coliphages, F-RNA bacteriophages, bacteriophages infecting Bacteroides fragilis, enteroviruses, adenoviruses and hepatitis A viruses. Most of the bacteriological analysis results were in accordance with the European Union standards. In all sites, bacteriophages were detected occasionally. Enteroviruses and adenoviruses were detected in 24 samples (10.26%) and 37 samples (15.81%) respectively. No samples were positive for the presence of hepatitis A virus. The overall data indicates that bathing sites are impacted by human faecal material. Both bacterial indicators and phages have low predictive capability for the presence of human viruses in coastal waters. None of the environmental parameters analysed was strongly related to the presence of the indicator organisms and viruses. Appropriate and effective administrative measures that should be taken into account may be considered in order to improve water quality and reduce public health risk.


2019 ◽  
Vol 1 ◽  
pp. 12 ◽  
Author(s):  
Lydia Mosi ◽  
Samuel Mawuli Adadey ◽  
Sandra Akoley Sowah ◽  
Charles Yeboah

Background: Sachet water, popularly known as “pure water” has become an invaluable entity in most Ghanaian households. Despite its importance, there is no extensive nationwide investigations on its wholesomeness for consumption. The aim of this study was to determine the microbiological quality of 41 brands of sachet water sampled in 16 districts across 5 regions in Ghana. Methods: The samples were analyzed for the presence of total and fecal coliform (Escherichia coli) using the Colilert*- 18 Test Kit. Results: Majority of the samples (56.09%) were excellent, 4.87% satisfactory and 14.63% suspicious. Ten samples (24.4%) were unsatisfactory. For the degree of fecal contamination, (85.56%) were satisfactory, four (9.76%) were suspicious, and two others (4.88%) were unsatisfactory. The contaminations observed could be attributed to poor sanitary conditions (during and/or after production) and failure of some production facilities to adhere to standard manufacturing practices. Conclusion: Our data suggest that microbiological quality sachet water from some sources have not yet attained levels that make it absolutely pure and wholesome for consumption in many areas.


2018 ◽  
Vol 84 (0) ◽  
Author(s):  
Lucélia Guimarães ◽  
Ana Clara dos Santos ◽  
Elka Ferreira ◽  
Débora Pereira ◽  
Francisca Costa

ABSTRACT: Fish are considered rich sources of nutrients. Health care throughout its production chain aims to ensure quality, minimizing the risks of transmission of foodborne diseases. In order to evaluate the microbiological quality of trahira fish (H. malabaricus), 40 samples were analysed for Most Probable Number (MPN) of coliforms at 45ºC, counts of aerobic mesophilic bacteria and Staphylococcus spp., identification of E. coli, Salmonella spp. and Aeromonas spp.. Analyses were conducted according to official methods, procedures, and recommendations. Microbiological results showed coliform values at 45 °C ranging from <3 to > 1.1 × 103 MPN/g, presence of E. coli in 14 (35%) samples, counts of mesophilic aerobic bacteria from 9 × 102 to 109 CFU/g and absence of coagulase positive Staphylococcus aureus. Salmonella spp. was detected in 2 (5%) samples, which is in disagreement with the standards required by the RDC Nº 12 of January, 2001 (ANVISA) regarding Aeromonas spp. In total, 36 (90%) samples were contaminated, 7 (19.4%) by A. cavie and 29 (80.6%) by A. hidrophila. The results of this research showed unsatisfactory hygienic and sanitary conditions of fish from the municipality of São Bento (MA), exposing consumers to the risk of foodborne diseases.


1986 ◽  
Vol 49 (7) ◽  
pp. 507-509 ◽  
Author(s):  
E. B. MARTÍNEZ ◽  
S. TESONE ◽  
F. QUEVEDO

Sixty-nine samples of liquid bovine rennet extract from several cheese-making plants were examined for microbiological quality. Wide differences were observed in the microbiological results, as well as in the pH, which ranged from 4.0 to 6.5, reflecting the manufacturing practices and sanitary conditions. The highest level of contamination was always caused by sporulated bacteria, both aerobic and anaerobic. Coliforms, considered to be enteric indicator bacteria were not detected, although halotolerant bacteria were found.


2011 ◽  
Vol 53 (3) ◽  
pp. 121-124 ◽  
Author(s):  
Rogério Pereira Xavier ◽  
Leonardo Pereira Siqueira ◽  
Fernando Antonio Chaves Vital ◽  
Francisca Janaina Soares Rocha ◽  
João Inácio Irmão ◽  
...  

Despite all efforts to store and reduce its consumption, water is becoming less inexhaustible and its quality is falling faster. Considering that water is essential to animal life, it is necessary to adopt measures to ensure its sanitary conditions in order to be fit for consumption. The aim of this study was to analyze the microbiological quality of drinking rainwater used by rural communities of Tuparetama, a small town located in Northeast Brazil. The study covered seven rural communities, totaling 66 households. In each household two samples were collected, one from a tank and the other from a clay pot located inside the home, resulting in 132 samples (tank plus clay pot). Approximately 90% of samples were below the standard recommended by the current legislation, being considered unfit for human consumption. Part of this high microbiological contamination of drinking rainwater could be related to the lack of sanitary education and of an adequate sewerage sanitation system.


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