scholarly journals PROXIMATE COMPOSITION AND HPLC-DAD ANALYSIS OF BIOACTIVE POLYPHENOLS IN LEAFY VEGETABLES CONSUMED IN THE DIET FOUND IN SOUTHERN PART OF BANGLADESH

2021 ◽  
Vol 18 (1) ◽  
pp. 27-36
Keyword(s):  
Proximate Composition ◽  
Antioxidant Activities ◽  
Chenopodium Album ◽  
Radical Scavenging ◽  
Syringic Acid ◽  
Leafy Vegetables ◽  
Total Phenolic ◽  
Tannin Content ◽  
Rural People ◽  
Methanolic Extracts

Four leafy vegetables, Phyla nodiflora, Amaranthus spinosus, Amaranthus viridis and Chenopodium album usually consumed by the Southern people of Bangladesh. In this study, we tested proximate composition and antioxidant potential of four leafy vegetables and further HPLC content of bioactive polyphenols in the most promising vegetable. The results revealed that they are rich in protein (12-17g/100g), carbohydrate (10- 20 g/100g) and fibre (29 - 44 g/100 g). The ash, glucose, sucrose and xylose contents were found within the ranges of 14-20 g/100 g, 35-77 mg/100 g, 32.79-68.72 mg/100 g and 3.79-7.71 mg/100 g, respectively. All the vegetables showed a lower content of lipid (0.73 – 1.47 g/100 g) and higher moisture content (81-85 g/100 g). The methanolic extracts of the vegetables were found to possess notable amount of total phenolic (11-60 mg GAE/g), total flavonoid (365-565 mg QE/g) and tannin content (28-49 mg TAE/g). All the samples showed significant DPPH free radical scavenging (IC50 53-1097 µg/ml) and hydrogen peroxide scavenging activity (IC50 41-96 µg/ml). Out of these four species, C. album was found to be the most promising leafy vegetable because of its high protein and fibre content, low lipid content and good antioxidant activities. HPLC-DAD analysis revealed the presence of 3,4-dihydroxy benzoic acid, catechol, vanillic acid, syringic acid, rutin hydrate, p-coumaric acid, trans-ferulic acid, rosmarinic acid and quercetin in C. album. The results of this study provide evidence for the importance of these leafy vegetables in improving the nutritional and health status of Southern rural people of Bangladesh.

scholarly journals Antioxidant Activity and Total Phenolic Content of 24 Lamiaceae Species Growing in Iran

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Omidreza Firuzi ◽  
Katayoun Javidnia ◽  
Maryam Gholami ◽  
Mohammad Soltani ◽  
Ramin Miri
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Methanolic Extracts ◽  
Ferric Reducing Antioxidant Power

The antioxidant activities of the methanolic extracts of 9 Salvia species and 15 other Lamiaceae plants growing in Iran were evaluated using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values ranged form 8.5 to 79.0 μM quercetin equivalents/g dry weight, and IC50 values in the DPPH assay from 115.7 to 1350.2 μg dry weight/mL. Salvia species showed the highest antioxidant activities. S. santolinifolia, S. eremophila and S. palestina, which have not been studied before, were the most active plants. These were more active than the previously studied species from this family, such as S. multicaulis and Marrubium vulgare. S. hydrangea and Gontscharovia popovii also showed high antioxidant activities. FRAP and DPPH assay results showed good correlations with the total phenolic contents of the plants, measured by the Folin-Ciocalteau assay (r2 = 0.925 and 0.799, respectively, p < 0.0001). In conclusion, our study shows that some Lamiaceae plants growing in Iran represent good potential sources of natural antioxidants useful for either prevention or treatment of oxidative stress-related diseases.

scholarly journals Effectiveness of Solvent Extraction on Phytochemicals and Antioxidant Activities from Fresh and Dried Wheatgrass

2021 ◽  
pp. 1-10
Author(s):  
Towkir Ahmed Ove ◽  
Anwara Akter Khatun ◽  
Said Bin Saifullah ◽  
Maruf Ahmed
Keyword(s):  
Solvent Extraction ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Chlorophyll Contents ◽  
Methanolic Extracts ◽  
Ferric Reducing Antioxidant Power

Aims: This study was intended to investigate and quantify the phytochemicals and the antioxidant activities of fresh and powdered wheatgrass. Study Design:  The experiment was performed by solvent extraction technique for determining the phytochemicals of wheatgrass samples. Place and Duration of Study: The study was conducted at the Food engineering laboratory in Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh between June 2018 and December 2018. Methodology: Fresh and powdered wheatgrass were used for the experiment. Methanol and ethanol solutions were separately utilized, in an attempt to assume the efficient solvent for extraction. UV-Vis spectrophotometric methods were employed in general throughout the study. Total phenolic contents (TPC), total flavonoid contents (TFC), DPPH scavenging activity, ferric reducing antioxidant power (FRAP) and the total chlorophyll contents of both solvent extracts were analyzed. Results: Phytochemicals and antioxidant activity were found lower in both solvent extracts of powdered wheatgrass than fresh leaves. Phenolic content and Chlorophyll were significantly (P < 0.05) higher in fresh samples (extracts). Due to heating, a significant decrease was observed in the DPPH radical scavenging activity of powdered extracts. Ferric Reducing Antioxidant Power (FRAP) was significantly (P < 0.05) higher in methanolic extracts than in ethanolic extracts. No significant (P > 0.05) difference was observed in total flavonoids contents. Conclusion: These findings endorsed that both solvents demonstrate competitive potentials to extract bioactive compounds from wheatgrass and its derivatives and hence can be chosen specifically according to the components.

scholarly journals Phenolic compounds and antioxidant activities of grape canes extracts from vineyards

2016 ◽  
Vol 14 (3) ◽  
pp. e0805 ◽  
Author(s):  
Yanlun Ju ◽  
Ang Zhang ◽  
Yulin Fang ◽  
Min Liu ◽  
Xianfang Zhao ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Low Cost ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Methanolic Extracts ◽  
Phenolic Components ◽  
Dpph Radical Scavenging ◽  
Gallic Acid Equivalent

Grape canes are the main agro-wastes from vineyards. This work studied the antioxidant activities of the defatted methanolic extracts (ME) of canes from 11 genotypes: 5 Vitis vinifera widely known cultivars and 6 Chinese wild varieties from three species (V. amurensis, V. davidii, and V. pentagona) and the antioxidant activities of the ME’s chloroform fractions (CF), ethyl acetate fractions (EAF) and water fractions (WF). Among ME and its three fractions, EAF’s total phenolic contents (TPC) and total flavonoid contents (TFC) were the highest, at 586 mg/g of gallic acid equivalent and 320 mg/g of quercetin equivalent, respectively. The antioxidant power of the fractions/extracts was in the order EAF > ME > WF > CF, based on the DPPH radical-scavenging power and ferric-reducing antioxidant activity, while the order was EAF > CF > WF >ME based on the β-carotene-linoleic acid bleaching activity. Methanolic extracts demonstrated the strongest Fe2+-chelating activity. The antioxidant activities of the extracts/fractions generally correlated with the TPC and TFC in all assays, except with the Fe2+-chelating test. Grape canes from V. davidii had the highest TPC, TFC and antioxidant activities compared with those from other grape species. Catechin, epicatechin and trans-resveratrol were the predominant phenolic components of fractions/extracts. In light of these valuable bioactivities, grape canes from annual pruning practice considered as waste material have good commercial potential for utilization as a promising natural antioxidant in the food, pharmaceutical and cosmetic industries, given its low cost and availability in large amounts.

scholarly journals Variation in Phenolic Compounds and Antioxidant Activity of Various Organs of African Cabbage (Cleome gynandra L.) Accessions at Different Growth Stages

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1952
Author(s):  
Sylvia Maina ◽  
Da Hye Ryu ◽  
Gaymary Bakari ◽  
Gerald Misinzo ◽  
Chu Won Nho ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Leafy Vegetables ◽  
Total Phenolic ◽  
Growth Stages ◽  
Breeding Programs ◽  
Cleome Gynandra ◽  
Strong Linear Correlation ◽  
Different Growth Stages

The presence of nutritional and health-benefiting compounds has increased awareness of orphan leafy vegetables such as Cleome gynandra (CG), whose phytochemicals vary among accessions and organs during growth. This study investigated the polyphenol accumulation and antioxidant activities (AOA) of eight CG accessions from the vegetative stage to the seed set stage. Plants were separated into leaves and stem (LS), flowers, and silique organs, and extracts were analyzed for total phenolic content (TPC), total flavonoid content (TFC), rutin and astragalin content, and AOA using 2,2-diphenyl-1-picrylhydrazyl-hydrate (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). There were significant interaction effects of growth stages and accessions that contributed to changes in compounds content and AOA. TPC accumulated in plant generative parts, whereas flavonoids accumulated in young plant organs. HPLC profiling revealed that rutin was the most abundant compound in all organs, with flowers having the highest levels, while astragalin was only found in flowers. Silique extracts, particularly accession KF-14, recorded the highest TPC, which corresponded to the strongest radical scavenging activity in ABTS and DPPH assays and a strong linear correlation. The germplasm contained accessions with significantly different and varying levels of bioactive compounds and AOA. These findings potentiate the exploitation of CG organs such as siliques for AOA, flowers for rutin and astragalin, and young shoots for flavonoids. Moreover, the significant accumulation of the compounds in particular accessions of the germplasms suggest that such superior accessions may be useful candidates in genetic breeding programs to improve CG vegetable.

scholarly journals An Investigation of Antibacterial and Antioxidant Activity of Nettle (Urtica dioica L.), Mint (Mentha piperita), Thyme (Thyme serpyllum) and Chenopodium album L. Plants from Yaylacık Plateau, Giresun, Turkey

2019 ◽  
Vol 7 (1) ◽  
pp. 73 ◽  
Author(s):  
Duygu Balpetek Külcü ◽  
Cavidan Demir Gökışık ◽  
Sinem Aydın
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Chenopodium Album ◽  
Radical Scavenging ◽  
Urtica Dioica ◽  
Mentha Piperita ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Hexane Extracts

Ethanol, chloroform and hexane extracts from plants namely Urtica dioica L., Mentha piperita, Thyme serpyllum and Chenopodium album L. were evaluated for their total phenolic and total flavonoid contents, antioxidant and antibacterial efficiencies. The antioxidant activities were screened utilizing DPPH radical scavenging activity, ABTS scavenging activity, CUPRAC activity and total antioxidant capacity. Antibacterial activity of the tested extracts was determined by disc diffusion and broth dilution methods. U. dioica and C. album extracts showed varying activities against the test bacteria. The hexane extracts of T. serpyllum and C. album showed the weakest copper reducing antioxidant capacity (CUPRAC) activity. 2,2-diphenyl-1picrylhydrazyl (DPPH) activity of the solvents are increased in the following order: Ethanol>Chloroform>Hexane. Our results revealed that all of the tested plants might be an alternative to synthetic antioxidant and antibacterial agents.

scholarly journals Bio-Prospecting of a Few Brown Seaweeds for Their Cytotoxic and Antioxidant Activities

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Rashmi C. Vinayak ◽  
A. S. Sabu ◽  
Anil Chatterji
Keyword(s):  
Metal Ion ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Brown Seaweeds ◽  
Methanolic Extracts

Methanolic extracts (MEs) of seven brown seaweeds occurring in the Indian coastal waters were screened for their cytotoxic and antioxidant properties following various assays. The methanolic extracts of seaweeds in the order ofDictyopteris australis > Spatoglossum variabile > Stoechospermum marginatum > Spatoglossum aspermumshowed significant cytotoxic activity. A very high DPPH radical scavenging activity was exhibited by the methanolic extracts prepared fromSt. marginatum, Padina tetrastromatica, Dictyopteris delicatulaandS. aspermum. The total phenolic content of the MEs varied from 13.19 ± 0.32 to 25.29 ± 0.445 gallic acid equivalents (mg g−1of methanolic extract). The reducing power assay indicated a dose dependency, at concentrations of 0.1, 0.5 and 1.0 and 2.0 mg mL−1of MEs and decreased in the following order:Butylated hydroxy toluene > P. tetrastromatica > D. delicatula > S. aspermum > S. variabile > S. marginatum > D. australis > S. marginatum. Furthermore,D. australis,S. aspermum, S. variabileandS. marginatumdemonstrated good metal ion chelating properties. All the above evidences suggest that, the antioxidant compounds found in brown seaweeds scavenge free radicals through effective intervention. This decisively promotes them as a potential source of natural antioxidants.

Antioxidant and Antibacterial Activities of Phytochemicals in Methanolic Extracts of Five Underutilized Leafy Vegetables

2021 ◽  
Vol 16 (8) ◽  
pp. 1-10
Author(s):  
Adarsha Raj ◽  
Bratati Sikdar ◽  
Alokesh Roy ◽  
Asish Kumar Mukhopadhyay ◽  
Sudipta Roy
Keyword(s):  
Antioxidant Activities ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Antibacterial Activities ◽  
Leafy Vegetables ◽  
Alternanthera Philoxeroides ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Methanolic Extracts ◽  
Therapeutic Uses

Leafy vegetables are important for their nutritive and medicinal values. However, many of them are less explored and underutilized. The present study evaluates the antioxidant and antibacterial activities present in five underutilized leafy vegetables such as Alternanthera philoxeroides, Boerhavia diffusa, Enydra(=Enhydra) fluctuans, Glinus oppositifolius and Suaeda maritima. Methanolic extracts from leafy edible parts of these plants possessed diverse phytochemicals. Of these plants, the polyphenol contents like total phenolic (TPC), total flavonoid (TFC) and total tannin (TTC) were the highest in E. fluctuans with significant variations concerning other plants. Also, the antioxidant activities of E. fluctuans were greater comparing other plants; the parameters were the total antioxidant activity (TAA), ferric reducing power assay and ability to scavenge DPPH, ABTS and superoxide radicals. The effect of varying extract-quantities revealed the highest antibacterial activity of the E. fluctuans extracts with substantial inhibition zones against six bacterial strains. Accordingly, the E. fluctuans extracts showed the lowest MIC (2.75±0.35 mg ml-1) and MBC (3.50±0.71 mg ml-1), albeit with significant variations with ampicillin. The study highlights that these plants are the untapped repertoires of natural antioxidants and antimicrobials for dietary and therapeutic uses.

scholarly journals Antibacterial, Antibiofilm and Antioxidant Activities of some Medicinal Plants from Pharmacopoeia of Tassili N’ajjer

2020 ◽  
Vol 14 (3) ◽  
pp. 1835-1844
Author(s):  
Abdelkader Benhelima ◽  
Olivier Vidal ◽  
Zohra Kaid-Omar ◽  
Rabea Sahki ◽  
Jean-Marie Lacroix
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Confocal Scanning Laser Microscopy ◽  
Total Phenolic ◽  
Systematic Research ◽  
Biofilm Inhibition ◽  
Methanolic Extracts ◽  
Cymbopogon Schoenanthus

Three plants adopted by nomads at Tassili n’ajjer (south Algeria) in traditional medicine namely, Cymbopogon schoenanthus, Anabasis articulata and Salvia chudaei, were analysed for theirs antibacterial, antibiofilm and antioxidant properties. Total flavonoid and phenolic contents were measured with 2% AlCl3 and Folin-Ciocalteu’s reagent method, respectively. The antibacterial propertie was investigated by measurement of MIC of plants extract inhibing bacterial proliferation. The antibiofilm propertie was calculated by fluorescent quantization of the DAPI labeled bacterial biomass fixed on the surface and by COMSTAT analysis of confocal scanning laser microscopy (CSLM) images. DPPH radical scavenging and β-carotene/linoleate bleaching methods were used to determine the antioxidant activities of the plants. Total phenolic content was ranged from 21.98 to 2.51 (gallic acid equivalents mg/g methanolic extracts) and the total flavonoid content ranged from 19.27 to 1.65 (catechin equivalent mg/g methanolic extracts). Antibacterial activity against four Gram positive bacteria was shown with plants extracts. The biofilm inhibition concentration of extracts decreasing 50% of biofilm cell density (BIC50) for Salvia chudaei and Cymbopogon schoenanthus extracts ranges from 1 to 10 µg/mL. CSLM images analysis revealed that both surface covering by germ and three dimensional development of the biofilm were reduced with plants extracts. For antioxidant activities, the methanol extracts of the plants evaluated showed low antioxidant activity, with a IC50 between 1.94 and 6.16 mg/ml. Thus, Our systematic research showed that this three common plants of Sahara desert has diversified phytochemicals possessing satisfying extent of antimicrobial, antibiofilm and antioxidant activities.

scholarly journals Chromatographic Separation of Breynia retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents from Bioactive Extracts

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5537
Author(s):  
Stefano Dall’Acqua ◽  
Kouadio Ibrahime Sinan ◽  
Irene Ferrarese ◽  
Stefania Sut ◽  
Kouadio Bene ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Flavonoid Glycosides ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Main Compound ◽  
Methanolic Extracts

Breynia retusa (Dennst.) Alston (also known as Cup Saucer plant) is a food plant with wide applications in traditional medicine, particularly in Ayurveda. Extracts obtained with four solvents (dichloromethane, methanol, ethyl acetate and water), from three plant parts, (fruit, leaf and bark) were obtained. Extracts were tested for total phenolic, flavonoid content and antioxidant activities using a battery of assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), total antioxidant capacity (TAC) (phosphomolybdenum) and metal chelating. Enzyme inhibitory effects were investigated using acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase and α-glucosidase as target enzymes. Results showed that the methanolic bark extract exhibited significant radical scavenging activity (DPPH: 202.09 ± 0.15; ABTS: 490.12 ± 0.18 mg Trolox equivalent (TE)/g), reducing potential (FRAP: 325.86 ± 4.36: CUPRAC: 661.82 ± 0.40 mg TE/g) and possessed the highest TAC (3.33 ± 0.13 mmol TE/g). The methanolic extracts were subjected to LC-DAD-MSn and NMR analysis. A two-column LC method was developed to separate constituents, allowing to identify and quantify forty-four and fifteen constituents in bark and fruits, respectively. Main compound in bark was epicatechin-3-O-sulphate and isolation of compound was performed to confirm its identity. Bark extract contained catechins, procyanidins, gallic acid derivatives and the sulfur containing spiroketal named breynins. Aerial parts mostly contained flavonoid glycosides. Considering the bioassays, the methanolic bark extract resulted a potent tyrosinase (152.79 ± 0.27 mg kojic acid equivalent/g), α-amylase (0.99 ± 0.01 mmol acarbose equivalent ACAE/g) and α-glucosidase (2.16 ± 0.01 mmol ACAE/g) inhibitor. In conclusion, methanol is able to extract the efficiently the phytoconstituents of B. retusa and the bark is the most valuable source of compounds.

scholarly journals Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2864
Author(s):  
Marwa Rezgui ◽  
Mabrouk Basma ◽  
Nuno Neng ◽  
José Manuel Nogueira ◽  
Leila Bettaieb Ben-Kaab ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Artemia Salina ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Methanolic Extracts ◽  
Marrubium Vulgare

Marrubium vulgare L., known as horehound, is a widespread and widely known plant that is used in beer breweries and also as a traditional remedy in Tunisia. In this study, methanolic extracts of plants harvested from five different locations were investigated for their antioxidant activities using three assays (ferric reducing power, radical scavenging activity, and β-carotene-linoleic acid bleaching assay) as well as the total phenolic content. The mineral composition of the plant was also investigated concerning the following elements: Fe, Mg, Ca, Cu, Zn, Mn, K, and three heavy metals, Ni, Pb, and Cd. Marrubiin, the major bioactive diterpenoid lactone, was quantified by NMR in the samples. The essential oils were obtained by hydrodistillation, and their radical scavenging activity was investigated. The toxicity of essential oils was evaluated against Artemia salina (the brine shrimp larva). The essential oil showed a weak radical scavenging activity and low toxicity. Data obtained from the five different locations showed that the antioxidant activity, as well as the total phenolic and marrubiin content, were strongly affected by the harvest sites. The metal content in the samples showed differences with the harvest location, but there was always a great abundance of calcium, magnesium, and potassium.

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