Antioxidant and Antibacterial Activities of Phytochemicals in Methanolic Extracts of Five Underutilized Leafy Vegetables

2021 ◽  
Vol 16 (8) ◽  
pp. 1-10
Author(s):  
Adarsha Raj ◽  
Bratati Sikdar ◽  
Alokesh Roy ◽  
Asish Kumar Mukhopadhyay ◽  
Sudipta Roy
Keyword(s):  
Antioxidant Activities ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Antibacterial Activities ◽  
Leafy Vegetables ◽  
Alternanthera Philoxeroides ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Methanolic Extracts ◽  
Therapeutic Uses

Leafy vegetables are important for their nutritive and medicinal values. However, many of them are less explored and underutilized. The present study evaluates the antioxidant and antibacterial activities present in five underutilized leafy vegetables such as Alternanthera philoxeroides, Boerhavia diffusa, Enydra(=Enhydra) fluctuans, Glinus oppositifolius and Suaeda maritima. Methanolic extracts from leafy edible parts of these plants possessed diverse phytochemicals. Of these plants, the polyphenol contents like total phenolic (TPC), total flavonoid (TFC) and total tannin (TTC) were the highest in E. fluctuans with significant variations concerning other plants. Also, the antioxidant activities of E. fluctuans were greater comparing other plants; the parameters were the total antioxidant activity (TAA), ferric reducing power assay and ability to scavenge DPPH, ABTS and superoxide radicals. The effect of varying extract-quantities revealed the highest antibacterial activity of the E. fluctuans extracts with substantial inhibition zones against six bacterial strains. Accordingly, the E. fluctuans extracts showed the lowest MIC (2.75±0.35 mg ml-1) and MBC (3.50±0.71 mg ml-1), albeit with significant variations with ampicillin. The study highlights that these plants are the untapped repertoires of natural antioxidants and antimicrobials for dietary and therapeutic uses.

scholarly journals COMPARATIVE STUDY ON THE ANTIOXIDANT ACTIVITIES OF METHANOLIC AND AQUEOUS EXTRACTS OF TERMINALIA BELLERICA

2019 ◽  
pp. 103-110
Author(s):  
RASNA GUPTA ◽  
RAM LAKHAN SINGH ◽  
ANKIT GUPTA
Keyword(s):  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Fruit Pulp ◽  
Terminalia Bellerica ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Methanolic Extracts ◽  
Better Than

Objective: The present study was undertaken to investigate the antioxidative activity of fruit pulp, seed and bark of T. bellerica. Methods: Methanol (70%) and water were used for the extraction, and the respective extracts were analyzed for total phenolic contents along with the antioxidant activities through different assays. Results: Amongst the tested extracts, methanolic extracts were found to be better than aqueous with the highest total phenolic contents (TPC). Methanolic fruit pulp showed lower IC50 for free radical (118.7 µg/ml), superoxide anion radical (77.65 µg/ml) and hydroxyl radical (73.76 µg/ml) scavenging activities. Methanolic fruit pulp also showed lower IC50 for lipid peroxidation (115.6 µg/ml) and ferric thiocyanate (184.98 µg/ml) assay than aqueous extracts in comparison to standard quercetin. Further, correlation between TPC and antioxidant studies revealed that phenolics are mainly responsible for antioxidant activity of T. bellerica. Conclusion: The current study suggests that the methanolic extract of T. bellerica could be a potential source of natural antioxidants for food and pharmaceutical companies.

scholarly journals Antioxidant Activity and Total Phenolic Content of 24 Lamiaceae Species Growing in Iran

2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Omidreza Firuzi ◽  
Katayoun Javidnia ◽  
Maryam Gholami ◽  
Mohammad Soltani ◽  
Ramin Miri
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Methanolic Extracts ◽  
Ferric Reducing Antioxidant Power

The antioxidant activities of the methanolic extracts of 9 Salvia species and 15 other Lamiaceae plants growing in Iran were evaluated using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values ranged form 8.5 to 79.0 μM quercetin equivalents/g dry weight, and IC50 values in the DPPH assay from 115.7 to 1350.2 μg dry weight/mL. Salvia species showed the highest antioxidant activities. S. santolinifolia, S. eremophila and S. palestina, which have not been studied before, were the most active plants. These were more active than the previously studied species from this family, such as S. multicaulis and Marrubium vulgare. S. hydrangea and Gontscharovia popovii also showed high antioxidant activities. FRAP and DPPH assay results showed good correlations with the total phenolic contents of the plants, measured by the Folin-Ciocalteau assay (r2 = 0.925 and 0.799, respectively, p < 0.0001). In conclusion, our study shows that some Lamiaceae plants growing in Iran represent good potential sources of natural antioxidants useful for either prevention or treatment of oxidative stress-related diseases.

Phytochemical and Biological Activities of five Turanecio Hamzaoglu (Asteraceae) Species from Turkey

2017 ◽  
Vol 13 (1) ◽  
pp. 49-55
Author(s):  
Sevil Albayrak ◽  
Ahmet Aksoy ◽  
Abit Yasar ◽  
Lutfiye Yurtseven ◽  
Umit Budak
Keyword(s):  
Therapeutic Potential ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Natural Antioxidants ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
In Vitro Assays ◽  
Methanolic Extracts ◽  
Promising Source

Objectives: In vitro biological activities of methanolic extracts of five Turanecio species have been studied. Materials and Methods: The phenolic compositions of the extracts were evaluated by the Folin- Ciocalteu assay and by HPLC analysis. Antioxidant activities were determined with two in vitro assays namely, DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging assay and phosphomolybdenum assay. The antimicrobial activities of the extracts were evaluated against 13 microorganisms. Results: T. hypochionaeus var. hypochionaeus was showed the highest DPPH inhibition with 88.84% at 100 μg·mL-1. All of the extracts were exerted high total antioxidant activities 128.00- 243.13 mg AAE g-1) and seem to be a promising source of natural antioxidants. The phenolic contents in the extracts varied from 26.17 to 60.99 mg·g-1 as gallic acid equivalent. Chlorogenic acid, caffeic acid and p- coumaric acid were the predominant constituents. The methanolic extracts revealed promising antibacterial activities against most bacteria. No activity was recorded against yeasts tested. Conclusion: The polyphenolic constituents appear to be responsible, at least in part, for the extract’s activities. The present study confirms that tested Turanecio species contains significant source of phenolics have antioxidant and antimicrobial activities and may have therapeutic potential.

scholarly journals Determination of Phenolic Compounds in Various Propolis Samples Collected from an African and an Asian Region and Their Impact on Antioxidant and Antibacterial Activities

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4589
Author(s):  
Soumaya Touzani ◽  
Hamada Imtara ◽  
Shankar Katekhaye ◽  
Hamza Mechchate ◽  
Hayat Ouassou ◽  
...  
Keyword(s):  
Cinnamic Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Photodiode Array Detection

The biological activities of propolis samples are the result of many bioactive compounds present in the propolis. The aim of the present study was to determine the various chemical compounds of some selected propolis samples collected from Palestine and Morocco by the High-Performance Liquid Chromatography–Photodiode Array Detection (HPLC-PDA) method, as well as the antioxidant and antibacterial activities of this bee product. The chemical analysis of propolis samples by HPLC-PDA shows the cinnamic acid content in the Palestinian sample is higher compared to that in Moroccan propolis. The results of antioxidant activity demonstrated an important free radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and reducing power assays) with EC50 values ranging between 0.02 ± 0.001 and 0.14 ± 0.01 mg/mL. Additionally, all tested propolis samples possessed a moderate antibacterial activity against bacterial strains. Notably, Minimum Inhibitory Concentrations (MICs) values ranged from 0.31 to 2.50 mg/mL for Gram-negative bacterial strains and from 0.09 to 0.125 mg/mL for Gram-positive bacterial strains. The S2 sample from Morocco and the S4 sample from Palestine had the highest content of polyphenol level. Thus, the strong antioxidant and antibacterial properties were apparently due to the high total phenolic and flavone/flavonol contents in the samples. As a conclusion, the activities of propolis samples collected from both countries are similar, while the cinnamic acid in the Palestinian samples was more than that of the Moroccan samples.

Contribution of organic bee pollen to the determination of the botanical origin of honey and its impact on its biological properties

2021 ◽  
Vol 18 ◽  
Author(s):  
Amar Otmani ◽  
Nadia Amessis-Ouchemoukh ◽  
Zina Mouhoubi-Tafinine ◽  
Kenza Tighlit ◽  
Ibtissam Redouan ◽  
...  
Keyword(s):  
Physicochemical Parameters ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Biological Properties ◽  
Total Phenolic ◽  
Botanical Origin ◽  
Sensitivity Testing ◽  
Physico Chemical

Background: Honey is a sweet and flavorful natural product which comes from a lot of nectar of medicinal plants. Objective: The aims of this study were to determine the botanical origin, physicochemical parameters and the antioxidant contents and antibacterial activities of some Algerian honeys. Method: The physico-chemical parameters of the honeys analyzed were measured using the methods established by the European Honey Commission. The antioxidant contents were determined using colorimetric assays, and the antioxidant activities were estimated using reducing power, DPPH, ABTS, and FRAP assays. Antibacterial activities were measured using sensitivity testing and minimal inhibitory concentration. Results: The majority of the samples analyzed were monofloral honeys (Fabaceae) and other medicinal plant pollens were shown (Asteraceae, Tiliaceae, Myrtaceae and Apiaceae). The physicochemical parameters of the honeys were in accordance with the legislation, and the analyses of proline and HMF confirmed their authenticity. The content of total phenolic compounds and total flavonoids ranged from 26 to 159 mg GAE/100 g and 10 to 43 mg EC/100 g, respectively. The analyzed honeys showed variable antioxidant activities that differed from one honey to another, and antibacterial activity tests showed that S. aureus and K. pneumoniae were the most sensitive strains with inhibition zones of 24 to 28 mm and 8 to 35 mm in diameter, respectively. Very high correlations were observed between color, antioxidants and antioxidant activities. Conclusion: This study confirmed the powerful properties of honey to trap free radicals and to inhibit bacterial growth that could be used as a therapeutic agent.

scholarly journals Phytochemical Analysis, Antioxidant and Antimicrobial Screening of Seriphidium Oliverianum Plant Extracts

Dose-Response ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 155932582110047
Author(s):  
Ali Abbas ◽  
Syed Ali Raza Naqvi ◽  
Muhammad Hidayat Rasool ◽  
Asma Noureen ◽  
Muhammad Samee Mubarik ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Dpph Scavenging

The aim of this study was to investigate the phytochemicals using reverse-phase high pressure liquid chromatography (RP-HPLC), antioxidant, antifungal and antibacterial activities of Seriphidium oliverianum stem extracts. The extraction was carried out by conventional shaking process (CSP) and ultrasonic assisted process (UAP). The highest total phenolic contents (97.85 ± 0.735 mg gallic acid equivalent (GAE)/g sample) and flavonoid contents (188.15 ± 0.53 mg catechin equivalent (CE)/g sample) were found in methanol extract obtained by CSP. Antioxidant activity was investigated using DPPH° scavenging assay and reducing power assay. Methanol extract using UAP showed the highest DPPH° scavenging activity (79.95% ± 1.80%) followed by methanol and butanol extracts obtained through CSP. Moreover, methanol extracts using CSP showed highest reducing activity (1.032 ± 0.0205 absorbance). In-vitro antimicrobial activity was studied using most common infection causing fungal and bacterial strains. Anti-fungal activity of methanol extract using CSP showed the highest zone of inhibition (10.5 mm) against F. avenaceum fungal strain, while aqueous extracts obtained through showed the highest antibacterial activity (22 ± 1.32 mm zone of inhibition) against S. aureus. The results showed that the methanol stem extract of S. oliverianum is a valued candidate for further screening and could be processed for in-vivo infection induced animal trials.

scholarly journals Effect of solvents on bioactive compounds and antioxidant activity of Padina tetrastromatica and Gracilaria tenuistipitata seaweeds collected from Bangladesh

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammad Khairul Alam Sobuj ◽  
Md. Ariful Islam ◽  
Md. Shoebul Islam ◽  
Md. Mohidul Islam ◽  
Yahia Mahmud ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Brown Seaweed ◽  
Reducing Power ◽  
Total Phenolic ◽  
Gracilaria Tenuistipitata ◽  
Padina Tetrastromatica ◽  
Methanolic Extracts

AbstractSeaweeds are now recognized as a treasure of bioactive compounds. However, the bioactivity of seaweed originating in Bangladesh is still unexplored. So, this study was designed to explore the secondary metabolites and antioxidant activities of solvent extracts of Padina tetrastromatica and Gracilaria tenuistipitata. Phytochemical screening and FTIR spectra confirm the diverse type of bioactive compounds. Antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), reducing power (RP), phosphomolybdenum, hydrogen peroxide and nitric oxide (NO) scavenging assays. Here, methanolic extract of P. tetrastromatica showed highest amount of total phenolic content (85.61 mg of GA/g), total flavonoid content (41.77 mg of quercetin/g), DPPH (77.07%), ABTS (77.65%), RP (53.24 mg AAE/g), phosphomolybdenum (31.58 mg AAE/g), hydrogen peroxide (67.89%) and NO (70.64%) assays compared to its methanolic extracts of G. tenuistipitata. This study concluded that methanol as a solvent extract of brown seaweed (P. tetrastromatica) exhibited bioactivity and antioxidant potentiality which will be useful for pharmacological as well as in functional food application.

scholarly journals Bio-Prospecting of a Few Brown Seaweeds for Their Cytotoxic and Antioxidant Activities

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Rashmi C. Vinayak ◽  
A. S. Sabu ◽  
Anil Chatterji
Keyword(s):  
Metal Ion ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Brown Seaweeds ◽  
Methanolic Extracts

Methanolic extracts (MEs) of seven brown seaweeds occurring in the Indian coastal waters were screened for their cytotoxic and antioxidant properties following various assays. The methanolic extracts of seaweeds in the order ofDictyopteris australis > Spatoglossum variabile > Stoechospermum marginatum > Spatoglossum aspermumshowed significant cytotoxic activity. A very high DPPH radical scavenging activity was exhibited by the methanolic extracts prepared fromSt. marginatum, Padina tetrastromatica, Dictyopteris delicatulaandS. aspermum. The total phenolic content of the MEs varied from 13.19 ± 0.32 to 25.29 ± 0.445 gallic acid equivalents (mg g−1of methanolic extract). The reducing power assay indicated a dose dependency, at concentrations of 0.1, 0.5 and 1.0 and 2.0 mg mL−1of MEs and decreased in the following order:Butylated hydroxy toluene > P. tetrastromatica > D. delicatula > S. aspermum > S. variabile > S. marginatum > D. australis > S. marginatum. Furthermore,D. australis,S. aspermum, S. variabileandS. marginatumdemonstrated good metal ion chelating properties. All the above evidences suggest that, the antioxidant compounds found in brown seaweeds scavenge free radicals through effective intervention. This decisively promotes them as a potential source of natural antioxidants.

scholarly journals PROXIMATE COMPOSITION AND HPLC-DAD ANALYSIS OF BIOACTIVE POLYPHENOLS IN LEAFY VEGETABLES CONSUMED IN THE DIET FOUND IN SOUTHERN PART OF BANGLADESH

2021 ◽  
Vol 18 (1) ◽  
pp. 27-36
Keyword(s):  
Proximate Composition ◽  
Antioxidant Activities ◽  
Chenopodium Album ◽  
Radical Scavenging ◽  
Syringic Acid ◽  
Leafy Vegetables ◽  
Total Phenolic ◽  
Tannin Content ◽  
Rural People ◽  
Methanolic Extracts

Four leafy vegetables, Phyla nodiflora, Amaranthus spinosus, Amaranthus viridis and Chenopodium album usually consumed by the Southern people of Bangladesh. In this study, we tested proximate composition and antioxidant potential of four leafy vegetables and further HPLC content of bioactive polyphenols in the most promising vegetable. The results revealed that they are rich in protein (12-17g/100g), carbohydrate (10- 20 g/100g) and fibre (29 - 44 g/100 g). The ash, glucose, sucrose and xylose contents were found within the ranges of 14-20 g/100 g, 35-77 mg/100 g, 32.79-68.72 mg/100 g and 3.79-7.71 mg/100 g, respectively. All the vegetables showed a lower content of lipid (0.73 – 1.47 g/100 g) and higher moisture content (81-85 g/100 g). The methanolic extracts of the vegetables were found to possess notable amount of total phenolic (11-60 mg GAE/g), total flavonoid (365-565 mg QE/g) and tannin content (28-49 mg TAE/g). All the samples showed significant DPPH free radical scavenging (IC50 53-1097 µg/ml) and hydrogen peroxide scavenging activity (IC50 41-96 µg/ml). Out of these four species, C. album was found to be the most promising leafy vegetable because of its high protein and fibre content, low lipid content and good antioxidant activities. HPLC-DAD analysis revealed the presence of 3,4-dihydroxy benzoic acid, catechol, vanillic acid, syringic acid, rutin hydrate, p-coumaric acid, trans-ferulic acid, rosmarinic acid and quercetin in C. album. The results of this study provide evidence for the importance of these leafy vegetables in improving the nutritional and health status of Southern rural people of Bangladesh.

scholarly journals Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2864
Author(s):  
Marwa Rezgui ◽  
Mabrouk Basma ◽  
Nuno Neng ◽  
José Manuel Nogueira ◽  
Leila Bettaieb Ben-Kaab ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Artemia Salina ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Methanolic Extracts ◽  
Marrubium Vulgare

Marrubium vulgare L., known as horehound, is a widespread and widely known plant that is used in beer breweries and also as a traditional remedy in Tunisia. In this study, methanolic extracts of plants harvested from five different locations were investigated for their antioxidant activities using three assays (ferric reducing power, radical scavenging activity, and β-carotene-linoleic acid bleaching assay) as well as the total phenolic content. The mineral composition of the plant was also investigated concerning the following elements: Fe, Mg, Ca, Cu, Zn, Mn, K, and three heavy metals, Ni, Pb, and Cd. Marrubiin, the major bioactive diterpenoid lactone, was quantified by NMR in the samples. The essential oils were obtained by hydrodistillation, and their radical scavenging activity was investigated. The toxicity of essential oils was evaluated against Artemia salina (the brine shrimp larva). The essential oil showed a weak radical scavenging activity and low toxicity. Data obtained from the five different locations showed that the antioxidant activity, as well as the total phenolic and marrubiin content, were strongly affected by the harvest sites. The metal content in the samples showed differences with the harvest location, but there was always a great abundance of calcium, magnesium, and potassium.

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