scholarly journals Aorta Fluorescence Imaging by Using Confocal Microscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Chun-Yang Wang ◽  
Jui-che Tsai ◽  
Ching-Cheng Chuang ◽  
Yao-Sheng Hsieh ◽  
Chia-Wei Sun
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Imaging ◽  
Fluorescence Detection ◽  
Vascular Endothelium ◽  
Biological Properties ◽  
Clinical Applications ◽  
Fluorescence Measurement ◽  
Cardiovascular Tissue ◽  
Wavelength Filter

The activated leukocyte attacked the vascular endothelium and the associated increase in VEcadherin number was observed in experiments. The confocal microscopic system with a prism-based wavelength filter was used for multiwavelength fluorescence measurement. Multiwavelength fluorescence imaging based on the VEcadherin within the aorta segment of a rat was achieved. The confocal microscopic system capable of fluorescence detection of cardiovascular tissue is a useful tool for measuring the biological properties in clinical applications.

Clinical Applications of In Vivo Confocal Microscopy in Keratorefractive Surgery

2021 ◽  
Vol 37 (7) ◽  
pp. 493-503
Author(s):  
Stephen Stewart ◽  
Yu-Chi Liu ◽  
Molly Tzu-Yu Lin ◽  
Jodhbir S. Mehta
Keyword(s):  
Confocal Microscopy ◽  
Clinical Applications ◽  
In Vivo Confocal Microscopy ◽  
Keratorefractive Surgery

Rapid detection and cellular fluorescence imaging of the TBI biomarker Let-7i using a DNA–AgNC nanoprobe

2019 ◽  
Vol 43 (21) ◽  
pp. 7997-8004 ◽  
Author(s):  
Xingmei Li ◽  
Leiming Han ◽  
Yadong Guo ◽  
Yunfeng Chang ◽  
Jie Yan ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Detection ◽  
Rapid Detection ◽  
Intracellular Imaging

Rapid fluorescence detection of Let-7i for TBI diagnosis and intracellular imaging have been studied using the multifunctional DNA–AgNCs.

scholarly journals Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Jorge Jesús Veloz ◽  
Marysol Alvear ◽  
Luis A. Salazar
Keyword(s):  
Flow Cytometry ◽  
Confocal Microscopy ◽  
Streptococcus Mutans ◽  
Metabolic Activity ◽  
Crystal Violet ◽  
Biofilm Formation ◽  
Biological Activities ◽  
Biological Properties ◽  
Alternative Methods ◽  
Flow Cytometry Analysis

Several biological activities have been reported for the Chilean propolis, among their antimicrobial and antibiofilm properties, due to its high polyphenol content. In this study, we evaluate alternative methods to assess the effect of Chilean propolis on biofilm formation and metabolic activity of Streptococcus mutans (S. mutans), a major cariogenic agent in oral cavity. Biofilm formation was studied by using crystal violet and by confocal microscopy. The metabolic activity of biofilm was evaluated by MTT and by flow cytometry analysis. The results show that propolis reduces biofilm formation and biofilm metabolic activity in S. mutans. When the variability of the methods to measure biofilm formation was compared, the coefficient of variation (CV) fluctuated between 12.8 and 23.1% when using crystal violet methodology. On the other hand, the CV ranged between 2.2 and 3.3% with confocal microscopy analysis. The CV for biofilm’s metabolic activity measured by MTT methodology ranged between 5.0 and 11.6%, in comparison with 1.9 to 3.2% when flow cytometry analysis was used. Besides, it is possible to conclude that the methods based on colored compounds presented lower precision to study the effect of propolis on biofilm properties. Therefore, we recommend the use of flow cytometry and confocal microscopy in S. mutans biofilm analysis.

scholarly journals Serum from Varicose Patients Induces Senescence-Related Dysfunction of Vascular Endothelium Generating Local and Systemic Proinflammatory Conditions

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Justyna Mikuła-Pietrasik ◽  
Paweł Uruski ◽  
Krzysztof Aniukiewicz ◽  
Patrycja Sosińska ◽  
Zbigniew Krasiński ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelium ◽  
Varicose Veins ◽  
Neutralizing Antibody ◽  
Biological Properties ◽  
Oxygen Species ◽  
Healthy Donors ◽  
Pai 1

Although the role of endothelium in varicose vein development is indisputable, the effect of the pathology on biological properties of endothelial cells remains unclear. Here we examined if the presence of varicose veins affects senescence of endothelial cells (HUVECs) and, if so, what will be the local and systemic outcome of this effect. Experiments showed that HUVECs subjected to serum from varicose patients display improved proliferation, increased expression of senescence marker, SA-β-Gal, and increased generation of reactive oxygen species (ROS), as compared with serum from healthy donors. Both increased SA-β-Gal activity and ROS release were mediated by TGF-β1, the concentration of which in varicose serum was elevated and the activity of which in vitro was prevented using specific neutralizing antibody. Senescent HUVECs exposed to varicose serum generated increased amounts of ICAM-1, VCAM-1, P-selectin, uPA, PAI-1, and ET-1. Direct comparison of sera from varicose and healthy donors showed that pathological serum contained increased level of ICAM-1, VCAM-1, P-selectin, uPA, and ET-1. Calendar age of healthy subjects correlated positively with serum uPA and negatively with P-selectin. Age of varicose patients correlated positively with ICAM-1, VCAM-1, and ET-1. Collectively, our findings indicate that the presence of varicose veins causes a senescence-related dysfunction of vascular endothelium, which leads to the development of local and systemic proinflammatory environment.

Single-excitation, dual-emission biomass quantum dots: preparation and application for ratiometric fluorescence imaging of coenzyme A in living cells

Nanoscale ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 9270-9275 ◽  
Author(s):  
Yong Cui ◽  
Rongjun Liu ◽  
Fanggui Ye ◽  
Shulin Zhao
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
Fluorescence Detection ◽  
Coenzyme A ◽  
Living Cells ◽  
Fluorescence Probes ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Dual Emissions

A distinctive approach is proposed to prepare biomass quantum dots (BQDs) with single-excitation and dual-emissions property. The obtained BQDs have been employed as the fluorescence probes for ratiometric fluorescence detection and imaging of coenzyme A in living cells.

scholarly journals Mesenchymal stem cells: biological properties and clinical applications

2010 ◽  
Vol 10 (10) ◽  
pp. 1453-1468 ◽  
Author(s):  
Ignacio García-Gómez ◽  
Gema Elvira ◽  
Agustín G Zapata ◽  
María L Lamana ◽  
Manuel Ramírez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Biological Properties ◽  
Clinical Applications

scholarly journals Mesenchymal Stem Cells Isolated from Adipose and Other Tissues: Basic Biological Properties and Clinical Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Hakan Orbay ◽  
Morikuni Tobita ◽  
Hiroshi Mizuno
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adult Stem Cells ◽  
Cell Types ◽  
Biological Properties ◽  
Clinical Applications ◽  
Therapeutic Tool ◽  
Adult Tissues ◽  
Clinical Benefits ◽  
Multiple Cell

Mesenchymal stem cells (MSCs) are adult stem cells that were initially isolated from bone marrow. However, subsequent research has shown that other adult tissues also contain MSCs. MSCs originate from mesenchyme, which is embryonic tissue derived from the mesoderm. These cells actively proliferate, giving rise to new cells in some tissues, but remain quiescent in others. MSCs are capable of differentiating into multiple cell types including adipocytes, chondrocytes, osteocytes, and cardiomyocytes. Isolation and induction of these cells could provide a new therapeutic tool for replacing damaged or lost adult tissues. However, the biological properties and use of stem cells in a clinical setting must be well established before significant clinical benefits are obtained. This paper summarizes data on the biological properties of MSCs and discusses current and potential clinical applications.

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