scholarly journals Impact of Salinity Stress on Germination and Growth of Pea (Pisum sativum L) Plants

2020 ◽  
Vol 35 (2) ◽  
pp. 146-159
Author(s):  
Amal F Ehtaiwwesh ◽  
Munira J Emsahel
Keyword(s):  
Seed Germination ◽  
Pisum Sativum ◽  
Salinity Stress ◽  
Growth Traits ◽  
Dry Weight ◽  
Branch Number ◽  
Pisum Sativum L ◽  
Salinity Levels ◽  
The Impact ◽  
Germination And Growth

The aim of the present study was to evaluate the effects of salinity stress on germination and growth of pea (Pisum sativum L) plants. A laboratory experiment was conducted to evaluate the effect of salinity stress on germination and growth of pea Pisum sativum L plant. Seeds of pea were sown in Petri dishes and pots and treated with four different levels of salinity (0, 50, 100, and 150mM NaCl) with completely randomized designs in four replications. Results revealed that seeds of pea were able to germinate at low salinity levels (NaCl 50mM NaCl) without a significant decrease in germination and growth traits, at the same time as a severe decrease in those traits were recorded at higher levels of salinity (100 and 150mM NaCl). The results indicated that seed germination and seedling establishment were inhibited due to the decrease of water potential, which results in the decline in water uptake by seeds, and seed germination was prevented by a high level of salinity stress (150mM NaCl). The results pointed out that germination percentage (GP), mean daily germination (MDG), germination speed (GS), and vigor index (SVI) varied under moderate and high salinity levels. All the studied parameters were reduced with increasing the NaCl level. The max and min GP, MDG, GS, and SVI were observed under control conditions (0mM NaCl) and highest salinity level (150mM NaCl) respectively. The same trend was seen in plant growth traits including: plant height, branch number, leaf number, leaf area, and shoot fresh and dry weight. The results provided important reference information for research on the impact of salinity on germination and growth of pea.

scholarly journals Characterization of Anatomical and Physiological Effects of Variegation Mutation on Grapevine

HortScience ◽  
2021 ◽  
pp. 1-7
Author(s):  
Jack Olson ◽  
Matthew Clark
Keyword(s):  
Growth Traits ◽  
Phenotypic Expression ◽  
Photochemical Efficiency ◽  
Leaf Tissue ◽  
Dry Weight ◽  
Wild Type ◽  
Branch Number ◽  
Carotenoid Concentration ◽  
Mesophyll Structure ◽  
The Impact

Variegation is a common trait in plants that characteristically displays white or off-colored plant tissue. In grapevine, leaf variegation is expressed as white and pale green leaf tissue resulting in plants that are stunted in growth and hindered in development. In this study, several experiments were performed to investigate the impact of this mutation has on the anatomy of grape leaves and physiology of the plant. Histological staining of variegated and nonvariegated leaf tissue transections showed alterations to the leaf palisade mesophyll structure that affected leaf tissue width. An assay quantifying leaf pigments was performed to compare chlorophyll and carotenoid concentrations in leaves between variegated and wild-type seedlings, which showed that variegated leaf samples had reduced chlorophyll and carotenoid concentration. Through fluorescence imaging, we determined that photochemical efficiency of photosystem II (PSII) is reduced in variegated seedlings. By growing variegated and wild-type plants under high, medium, and low light intensities that variegated plants exposed to higher light intensity reduces the phenotypic expression of the variegation trait. Also, we found variegated plants to have significant reductions in growth traits such as plant height, leaf number, branch number, and dry weight compared with wild-type phenotype plants. Overall, our experiments revealed the variegation mutation altered normal leaf development causing significant effects to grapevine physiology.

scholarly journals Assessment of Morpho-Physiological, Biochemical and Antioxidant Responses of Tomato Landraces to Salinity Stress

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 696
Author(s):  
Reem H. Alzahib ◽  
Hussein M. Migdadi ◽  
Abdullah A. Al Ghamdi ◽  
Mona S. Alwahibi ◽  
Abdullah A. Ibrahim ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salinity Stress ◽  
Dry Weight ◽  
Proline Content ◽  
Enzymatic Antioxidants ◽  
Sod Activity ◽  
Carotene Content ◽  
Antioxidants Activity ◽  
Solanum Lycopersicum L ◽  
Salinity Levels

Understanding salt tolerance in tomato (Solanum lycopersicum L.) landraces will facilitate their use in genetic improvement. The study assessed the morpho-physiological variability of Hail tomato landraces in response to different salinity levels at seedling stages and recommended a tomato salt-tolerant landrace for future breeding programs. Three tomato landraces, Hail 548, Hail 747, and Hail 1072 were tested under three salinity levels: 75, 150, and 300 mM NaCl. Salinity stress reduced shoots’ fresh and dry weight by 71% and 72%, and roots were 86.5% and 78.6%, respectively. There was 22% reduced chlorophyll content, carotene content by 18.6%, and anthocyanin by 41.1%. Proline content increased for stressed treatments. The 300 mM NaCl treatment recorded the most proline content increases (67.37 mg/g fresh weight), with a percent increase in proline reaching 61.67% in Hail 747. Superoxide dismutase (SOD) activity decreased by 65% in Hail 548, while it relatively increased in Hail 747 and Hail 1072 treated with 300 mM NaCl. Catalase (CAT) activity was enhanced by salt stress in Hail 548 and recorded 7.6%, increasing at 75 and 5.1% at 300 mM NaCl. It revealed a reduction in malondialdehyde (MDA) at the 300 mM NaCl concentration in both Hail 548 and Hail 1072 landraces. Increasing salt concentrations showed a reduction in transpiration rate of 70.55%, 7.13% in stomatal conductance, and 72.34% in photosynthetic rate. K+/Na+ ratios decreased from 56% for 75 mM NaCl to 85% for 300 mM NaCl treatments in all genotypes. The response to salt stress in landraces involved some modifications in morphology, physiology, and metabolism. The landrace Hail 548 may have better protection against salt stress and observed protection against reactive oxygen species (ROS) by increasing enzymatic “antioxidants” activity under salt stress.

Some Seed Traits and Their Relationships to Seed Germination and Field Emergence in Pea (Pisum sativum L.)

2004 ◽  
Vol 3 (4) ◽  
pp. 243-246 ◽  
Author(s):  
Erkut Peksen ◽  
Aysun Peksen . ◽  
Hatice Bozoglu . ◽  
Ali Gulumser .
Keyword(s):  
Seed Germination ◽  
Pisum Sativum ◽  
Seed Traits ◽  
Pisum Sativum L ◽  
Field Emergence

Evaluation of the Superabsorbent Effect on the Yield and Some Growth Factors of Pisum sativum L. under Drought Stress Conditions

2019 ◽  
Vol 10 (2) ◽  
pp. 106-114
Author(s):  
Farahnaz A. Norodinvand ◽  
Davoud K. Dehkordi ◽  
Aslan Egdernezhad
Keyword(s):  
Growth Factors ◽  
Drought Stress ◽  
Pisum Sativum ◽  
Treatment Plan ◽  
Dry Weight ◽  
Stress Conditions ◽  
Control Treatment ◽  
Significant Information ◽  
Superabsorbent Hydrogel ◽  
Pisum Sativum L

Background: Deficit irrigation is an optimum technique for producing products under drought stress conditions. The superabsorbent hydrogel is a hydrophilic polymer with cross-linked 3-D hydrophilic nets that are able to take up and keep noteworthy values of water and aquatic liquids. Recently published patents have provided significant information about the superabsorbent application in agriculture. Objective: The aim of this study was to investigate the superabsorbent effect on the yield and some of the growth factors of Pisum sativum L. under drought stress conditions. Methods: The experimental factors included: irrigation treatments at two levels of 100% and 75% water requirement by the plant. The second factor included the levels of superabsorbent application which included three levels of control treatment, potting soil with a weight percentage of 0.5 and 1. The third factor was the location of the superabsorbent application. Results: According to the results, the largest root length value corresponded to I2S1U treatment plan, the largest root dry weight value corresponded to I1S2U treatment plan, the largest plant dry weight corresponded to I2S1U treatment plan, the largest grain dry weight corresponded to I2S1U treatment plan, the largest number of grains per pod corresponded to I2S2U treatment plan and the largest number of pods per plant corresponded to I2S1U treatment plan which were significant at 5% level. Conclusion: It was concluded that the presence of the superabsorbent at the lower end of the pot was effective in providing water and nutrients for the plant root.

scholarly journals Screening of jute and kenaf varieties for salinity tolerance

2018 ◽  
Vol 6 (2) ◽  
pp. 214
Author(s):  
Md. Isfatuzzaman Bhuyan ◽  
K M. Mehadi Hassan ◽  
Nowrose Jahan Lipi ◽  
Md Rafiq Uddin ◽  
Md Monirul Islam ◽  
...  
Keyword(s):  
Plant Height ◽  
Salinity Stress ◽  
Salinity Tolerance ◽  
Dry Weight ◽  
Germination Inhibition ◽  
Germination Test ◽  
Salinity Levels ◽  
Total Plant ◽  
Number Of Leaves ◽  
Total Dry Weight

A study was conducted in the Department of Agronomy, Bangladesh Agricultural University (BAU), Mymensingh from April to August 2012 to examine the salinity tolerance of eight jute varieties (CVE-3, C-83, CVL-1, BJC-7370, O-795, O-9897, OM-1, O-72) and two kenaf varieties (HC-95 and HC-2). Initially germination of these varieties were evaluated under six salinity levels viz. 0mM, 20mM, 40mM, 60mM, 80mM, and 100mM NaCl in the seed laboratory of the Department of Agronomy. Afterwards, all the varieties was grown in pots in the net house under four salinity levels viz. 0mM, 25mM, 50mM, and 75mM. The results from the germination study revealed that under control condition (0mM NaCl) all the jute varieties showed germination more than 80% both at 7 and at 14 days after seed sowing, whereas kenaf varieties had germination a little less than 80%. Among the jute varieties, O-72 showed the highest germination (92%), which was statistically similar with those of OM-1(91%), O-795 (90%), and C-83(87%). Salinity stress decreased germination drastically in all of the jute and kenaf varieties. A salinity level of 100mM caused the highest germination inhibition (74.70%) in jute variety CVL-1, which was very close to those of BJC-7370 and O-72. On the other hand, the lowest germination inhibition (51.11%) was recorded in jute variety O-795. The results of the pot trail showed that the plant characters of jute and kenaf varieties were affected significantly by salinity stress. All the varieties produced their respective plant height, number of leaves per plant, and plant dry weight under control condition (no salinity). Among these varieties, CVE-3 produced the highest plant height (145.2cm), and total dry weight (22.55g), whereas O-72 produced the highest number of leaves per plant (24.67). All these plant characters decreased sharply due to salinity stress irrespective of variety. However, the rate of decrease of plant characters occurred differentially in the jute and kenaf varieties. The highest rate of decrease in number of leaves (74.22%) was found from the variety CVE-3, whereas the lowest one was recorded from the variety HC-2 (51.68%). Salinity stress caused the highest decrease in total plant dry weight (73.68%) in the variety CVL-1 and plant height (73.64%) also in the variety CVL-1. On the contrary, the lowest decrease in plant dry weight (50.99%) was found in the variety O-9897 and plant height also in variety O-9897 (50.88%). Based on the results from germination test and pot trail, it can be inferred that jute variety O-9897 appeared to be the most salt tolerant followed by O-795, HC-2, HC-95, CVE-3, O-72, C-83, BJC-7370, OM-1, and CVL-1.    

scholarly journals Physiological and Biochemical Characterization of the GABA Shunt Pathway in Pea (Pisum sativum L.) Seedlings Under Drought Stress

Horticulturae ◽  
2021 ◽  
Vol 7 (6) ◽  
pp. 125
Author(s):  
Nisreen A. AL-Quraan ◽  
Zakaria I. Al-Ajlouni ◽  
Nima F. Qawasma
Keyword(s):  
Drought Stress ◽  
Pisum Sativum ◽  
Water Availability ◽  
Dry Weight ◽  
Gaba Shunt ◽  
Pisum Sativum L ◽  
Pea Seedlings ◽  
Mda Content ◽  
Carbohydrate Levels

The physiological and biochemical role of the γ-aminobutyric acid (GABA) shunt pathway in green pea seedlings (Pisum sativum L.) was studied in response to soil water holding capacity levels: 80%, 60%, 40%, 20%, and 10% grown under continuous light at 25 °C for 7 days and 14 days, separately. Characterization of seeds germination pattern, seedlings growth (plant height, fresh and dry weight, and chlorophyll contents), GABA shunt metabolite (GABA, glutamate, and alanine) levels, total protein and carbohydrate levels, and oxidative damage (MDA level) were examined. Data showed a significant effect of drought stress on seed germination, plant growth, GABA shunt metabolites level, total protein and carbohydrate contents, and MDA level. A significant decline in seed germination percentage was recorded at a 20% drought level, which indicated that 20% of soil water holding capacity is the threshold value of water availability for normal germination after 14 days. Seedling fresh weight, dry weight, and plant height were significantly reduced with a positive correlation as water availability was decreased. There was a significant decrease with a positive correlation in Chl a and Chl b contents in response to 7 days and 14 days of drought. GABA shunt metabolites were significantly increased with a negative correlation as water availability decreased. Pea seedlings showed a significant increase in protein content as drought stress was increased. Total carbohydrate levels increased significantly when the amount of water availability decreased. MDA content increased slightly but significantly after 7 days and sharply after 14 days under all water stress levels. The maximum increase in MDA content was observed at 20% and 10% water levels. Overall, the significant increases in GABA, protein and carbohydrate contents were to cope with the physiological impact of drought stress on Pisum sativum L. seedlings by maintaining cellular osmotic adjustment, protecting plants from oxidative stress, balancing carbon and nitrogen (C:N) metabolism, and maintaining cell metabolic homeostasis and cell turgor. The results presented in this study indicated that severe (less than 40% water content of the holding capacity) and long-term drought stress should be avoided during the germination stage to ensure proper seedling growth and metabolism in Pisum sativum L.

scholarly journals Seed germination and seedling emergence of velvetleaf (Abutilon theophrasti) and Barnyardgrass (Echinochloa crus-galli)

2013 ◽  
Vol 31 (2) ◽  
pp. 259-266 ◽  
Author(s):  
A. Sadeghloo ◽  
J. Asghari ◽  
F. Ghaderi-Far
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Salinity Stress ◽  
Cropping Systems ◽  
Seedling Emergence ◽  
Abutilon Theophrasti ◽  
Medium Ph ◽  
Wide Range ◽  
Salinity Levels ◽  
Effects Of Temperature

Abutilon theophrasti and Barnyardgrass (Echinochloa crus-galli) are major weeds that affect cropping systems worldwide. Laboratory and greenhouse studies were conducted to determine the effects of temperature, pH, water and salinity stress, and planting depth on seed germination and seedling emergence of Velvetleaf and Barnyardgrass. For Velvetleaf, the base, optimum and ceiling germination temperatures were estimated as 5, 35 and 48 ºC, respectively. Seed germination was sensitive to drought stress and completely inhibited by a potential of -0.6 MPa, but it was tolerant to salinity. Salinity stress up to 45 mM had no effect on the germination of Velvetleaf, but germination decreased with increasing salt concentration. Drought and salinity levels for 50% inhibition of maximum germination were -0.3 MPa and 110 mM, respectively. Seed germination of Velvetleaf was tolerant to a wide range of pH levels. For Barnyardgrass, the base, optimum and ceiling germination temperatures were estimated as 5, 38 and 45 ºC, respectively. Seed germination was tolerant to drought stress and completely inhibited by a potential of -1.0 MPa. Salinity stress up to 250 mM had no effect on seed germination. Drought and salinity levels for 50% inhibition of maximum germination were -0.5 MPa and 307 mM, respectively. A high percentage of seed germination was observed at pH=5 and decreased to 61.5% at acidic medium (pH 4) and to 11% at alkaline medium (pH 9). Maximum seedling emergence of Velvetleaf and Barnyardgrass occurred when the seeds were placed on the surface of the soil or at a depth of 1 cm.

scholarly journals Mahameru Soybean (Glycine max) Cultivar, High Salinity Tolerant

2018 ◽  
Vol 10 (1) ◽  
pp. 23-31
Author(s):  
Juwarno Juwarno ◽  
Tata Brata Suparjana ◽  
Muachiroh Abbas
Keyword(s):  
Chlorophyll A ◽  
Salinity Stress ◽  
High Salinity ◽  
Dry Weight ◽  
Salinity Treatment ◽  
Soybean Production ◽  
Anatomy And Physiology ◽  
Salinity Levels ◽  
Chlorophyll A And B ◽  
Soybean Plants

Mahameru cultivar is high salinity tolerant cultivar. The previous study result showed Mahameru cultivar could tolerate 140mM NaCl, but Cilacap Coast salinity levels often reaching 200mM NaCl. A research of salinity stress on Mahameru cultivar at 200 mM NaCl have not conducted yet. Therefore to conduct the research of Mahameru at high salinity stress to obtained high salinity tolerant soybean cultivar.   The observed variables are anatomy (epidermis thickness, the density of stomata and trichomes, palisade thickness) physiology (the dry weight of roots and canopy, the content of chlorophyll a and b) Production (whole pod, total filled pod, total empty pod, weight per one-hundred beans). The salinity treatment was 0, 50,100, 150, 200 mM NaCl given at three days before planting and twenty-one days after planting. The data of anatomy and physiology was taken at forty-five days after planting. The production data was taken when soybean plants turned brown. The result indicates that salinity affects anatomy characteristic of leaf, higher the salinity increasing epidermis thickness and the density of stomata and trichomes. Salinity affected the content of chlorophyll a and b. Higher the salinity increased the content of chlorophyll a and b. Salinity did not affect soybean production. Based on this study Mahameru cultivar is resistant to salinity up to 200 mM NaCl. The benefit of this research help to enhance national soybean production with utilization coastal land for soybean planting Mahameru cultivar.         

scholarly journals Impact of seed size on seeds viability, vigor and storability of Hordeum vulgare (L.)

2018 ◽  
Author(s):  
M. Massimi
Keyword(s):  
Seed Germination ◽  
Seed Size ◽  
Seed Quality ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Germination Percentage ◽  
Seedling Vigor ◽  
Hordeum Vulgare L ◽  
Vigor Index ◽  
The Impact

Seed size may influence seed germination, and seedling vigor. Few investigations are available about the effect of seed size on barley seedling vigor in Jordan. The present study was designed to investigate the impact of seed size on germination percentage, seedling dry weight, seedling vigor index, and germination percentage after accelerated aging in barley. Three seed sizes i.e. large, medium, and small (having diameter of > 2.75 millimeter, 2.5 - 2.75 millimeter, and less than 2.5 millimeter, respectively) were tested in the experiment. Seed quality for different seed size categories was evaluated in the laboratory by measuring seed germination, germination after accelerated aging, as well seedling dry weight and vigor index. Results showed significant differences for large seeds in germination percentages, seedling dry weight and vigor index. It may be concluded that large seed size of barley showed best quality.

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