Antral follicles population in heifers and cows of Nelore and Girolando breeds

The aim of this study was to evaluate ovarian antral follicle populations (OAFP) of Nelore and Girolando breed heifers (12–18 months old) and cows (24–60 months old). Animals were assigned to four groups: (1) Nelore cows (n = 18), (2) Girolando cows (n = 20), (3) Nelore heifers (n = 7), and (4) Girolando heifers (n = 7). Cows were treated to synchronize follicular wave emergence by implantation of an intravaginal device containing 1.9 g of progesterone, as well as intramuscular administration of 2 mg of estradiol benzoate and 25 mg of dinoprost. This synchronization treatment was administered at a random day of the estrous cycle of each cow, designated D0. Intravaginal devices were removed on D7, and on D11, OAFP counts were performed by transvaginal ovarian ultrasound. For each cow, all follicles ?3 mm in diameter were counted in both ovaries and counts were performed three times at 35-day intervals. Counts were also obtained from heifers, but these animals were not treated for synchronization of follicular wave emergence. Analysis of variance (ANOVA) with Tukey’s test and Pearson’s correlation test were used to compare mean OAFPs between counts as well as mean OAFPs between breed and age groups. No differences were observed in mean OAFPs between Nelore and Girolando cows (30.9 vs. 26.7, respectively; P > 0.05) or heifers (16.2 vs. 18.1, respectively; P > 0.05). However, within each breed, there were differences in mean OAFPs between heifers and cows (for Nelore cattle: 16.2 and 30.9, respectively; for Girolando cattle: 18.1 and 26.7, respectively; both P < 0.05). In conclusion, OAFPs were similar between Nelore and Girolando breeds and were influenced by age. Furthermore, we observed a high correlation for individual animals between the mean numbers of follicles counted in both ovaries and total number of follicles counted in either the right or left ovary, indicating that the evaluation of a single ovary is sufficient to estimate the OAFP of an individual.

Download Full-text

Histomorphometric Study of the Glomeruli of the Kidney in Bangladeshi Population

Journal of Bangladesh Society of Physiologist ◽

10.3329/jbsp.v9i1.22788 ◽

2015 ◽

Vol 9 (1) ◽

pp. 11-16

Author(s):

Fatema Johora ◽

Abu Sadat Mohammad Nurunnabi ◽

Sunjida Shahriah ◽

Rukshana Ahmed ◽

Shamim Ara

Keyword(s):

Left Kidney ◽

Age Groups ◽

Population Based ◽

Cross Sectional ◽

Medical College ◽

Histomorphometric Study ◽

The Mean ◽

Group 10 ◽

Bangladeshi Population ◽

The Right

Background: Controversies still prevail on glomerular changes of kidney whether due to normal aging or its association with diseases Objective: The aim of the present study was to see the variation in number and size of the glomeruli of kidney with increasing age in a Bangladeshi population based on autopsy. Methods: This cross-sectional, descriptive study was done in the Department of Anatomy, Dhaka Medical College, Dhaka, from July 2008 to June 2009, based on collection of 140 post mortem human kidneys collected from 70 unclaimed dead bodies from the morgue. All the samples were divided into three age-groups: 10-19 years, 20-39 years and 40-59 years. Histological slides were prepared by using routine Harris Haematoxylin and Eosin (H & E) stain. The number of glomeruli was measured by point counting technique, while the size (diameter) was measured by using ocular and stage micrometer. Results: The mean ± SE number of glomeruli per sq. mm found in the right and left kidney were 8.45±0.52 and 8.67±0.80 in group 10-19 years, 9.90±0.42 and 9.92±0.47 in 20-39 years, and 8.52±0.18 and 8.55±0.16 in 40-59 years respectively. Besides, the size (mean ± SE diameter) of glomeruli was found in the right and left kidney were 43.96±3.01ìm and 143.92±2.90ìm in group 10-19 years, 153.69±5.18ìm and 153.61±5.24ìm in 20-39 years, and 140.48±0.95ìm and 140.78±0.88ìm in 40-59 years respectively. Conclusion: No difference was found in number and size of glomeruli between right and left kidney in any group. Similarly, no difference was also evident among different age groups. DOI: http://dx.doi.org/10.3329/jbsp.v9i1.22788 Bangladesh Soc Physiol. 2014, June; 9(1): 11-16

Download Full-text

Effect of exogenous gonadotrophins on ovarian morphology and oocyte maturation in the southern hairy nosed wombat Lasiohinus latifrons during the breeding season

Reproduction Fertility and Development ◽

10.1071/rd05047 ◽

2006 ◽

Vol 18 (4) ◽

pp. 477 ◽

Cited By ~ 9

Author(s):

C. H. McDonald ◽

D. A. Taggart ◽

W. G. Breed ◽

G. V. Druery ◽

G. A. Shimmin ◽

...

Keyword(s):

Oocyte Maturation ◽

Follicular Development ◽

Germinal Vesicle ◽

Control Group ◽

Antral Follicles ◽

Nuclear Maturation ◽

Significant Difference ◽

Ovarian Morphology ◽

The Mean ◽

The Right

The effect of the exogenous administration of porcine follicle-stimulating hormone (pFSH) and pregnant mare serum gonadotrophin (PMSG) on ovarian follicular development and oocyte maturation in the southern hairy nosed wombat Lasiorhinus latifrons was investigated. Three experimental groups were administered pFSH at various doses and for different treatment lengths, followed by 25 mg porcine luteinising hormone (pLH) 12 h after the last dose of pFSH. Another group was given PMSG followed 72 h later by 25 mg pLH. Animals were killed 24 h after pLH. The left ovary was fixed for histology and the morphology of the antral follicles was determined, whereas follicular oocytes in the right ovary were aspirated, fixed, stained with 4′,6′-diamidino-2-phenylindole, and viewed for nuclear maturation. There was no significant difference in the mean number of ovarian follicles >1 mm, or in the size class of follicles assessed between control and experimental groups. However, a trend was observed suggesting a possible increase in follicles >3.0 mm in experimental groups compared with control animals. In all females administered exogenous porcine gonadotrophins, but not controls, some of the mural granulosa cells of large tertiary antral follicles had markedly enlarged nuclei (approximately 14 µm in diameter). All oocytes from the control group remained at the germinal vesicle stage, whereas approximately 40% of oocytes retrieved from the pFSH groups and 82.4% retrieved from the PMSG-primed animals had undergone germinal vesicle break down, with a small number reaching meiosis II. The present study shows that exogenous administration of either pFSH or PMSG to hairy nosed wombats can induce follicular growth and oocyte maturation. Such findings could be useful in the development of reproductive technology in this species.

Download Full-text

Follicle Structure Influences the Availability of Oxygen to the Oocyte in Antral Follicles

Computational and Mathematical Methods in Medicine ◽

10.1155/2011/287186 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 12

Author(s):

A. R. Clark ◽

Y. M. Stokes

Keyword(s):

Mathematical Model ◽

Dissolved Oxygen ◽

Oxygen Concentration ◽

Oxygen Transport ◽

Follicular Fluid ◽

Antral Follicle ◽

Antral Follicles ◽

Cumulus Oocyte Complex ◽

The Mean ◽

Size And Structure

The ability of an oocyte to successfully mature is highly dependent on intrafollicular conditions, including the size and structure of the follicle. Here we present a mathematical model of oxygen transport in the antral follicle. We relate mean oxygen concentration in follicular fluid of bovine follicles to the concentration in the immediate vicinity of the cumulus-oocyte complex (COC). The model predicts that the oxygen levels within the antral follicle are dependent on the size and structure of the follicle and that the mean level of dissolved oxygen in follicular fluid does not necessarily correspond to that reaching the COC.

Download Full-text

Morphometric study of the size of the postmortem human kidney

Journal of Bangladesh Society of Physiologist ◽

10.3329/jbsp.v15i2.50919 ◽

2020 ◽

Vol 15 (2) ◽

pp. 61-67

Author(s):

Fatema Johora ◽

Abu Sadat Mohammad Nurunnabi ◽

Dilruba Siddiqua ◽

Hasna Hena ◽

Shamim Ara

Keyword(s):

Young Adult ◽

Left Kidney ◽

Age Groups ◽

Morphometric Study ◽

Age Related ◽

Study Results ◽

Group A ◽

The Mean ◽

The Right ◽

Age Related Changes

Background: Changes in the size of the kidney are evident in humans, as age progresses. Objective: To see the age-related changes in the morphological dimensions of the kidney in a sample of the Bangladeshi population. Methods: This crosssectional study was done in the Department of Anatomy, Dhaka Medical College, Dhaka, from July 2008 to June 2009, based on a collection of 140 postmortem human kidneys collected from 70 unclaimed dead bodies from the morgue of the same institution. All the samples of kidney were divided into three age groups, including A (10-19 years), B (20-39 years) and C (40-59 years). The length, breadth and thickness of all the kidneys were measured by using a slide calipers and recorded. Data were expressed as mean±SD. For statistical analysis, independent sample t test and one way ANOVA was used. Results: The length of the right and left kidneys found were 8.72±0.25 cm and 9.28±0.12 cm; 9.73±0.35 cm and 10.31±0.41 cm; 9.68±0.21 cm and 10.24±0.06 cm in group A, B and C respectively. The breadth of the right and left kidneys found were 4.32±0.09 cm and 4.22±0.11 cm; 4.74±0.29 cm and 4.55±0.28 cm; 4.61±0.21 cm and 4.44±0.21 cm in group A, B and C respectively. The thickness of the right and left kidneys found were 2.84±0.10 cm and 2.64±0.05 cm; 3.31±0.16 cm and 3.11±0.10 cm; 3.17±0.07 cm and 3.11±0.10 cm in group A, B and C respectively. The mean length of the left kidneys was found significantly greater than that of the right , whereas the mean breadth and the thickness of the right kidneys were found greater than that of the left kidney in all age groups. Moreover, age related changes were significant in all dimensions (length, breadth and thickness) of the kidney when compared between group A & B and A & C. Conclusion: This study results concluded that the length of the left kidney was greater than that of the right, but the breadth and the thickness of the right kidney were greater than that of the left one in all age groups. In addition, age related changes in all dimensions (i.e. length, breadth and thickness) of the kidneys were evident in middle age versus young adult and older adult versus young adult. J Bangladesh Soc Physiol. 2020, December; 15(2): 61-67

Download Full-text

Histological study of proportion of cortex and medulla of the ovary in Bangladeshi women

Bangladesh Medical Journal ◽

10.3329/bmj.v44i1.26337 ◽

2016 ◽

Vol 44 (1) ◽

pp. 8-10

Author(s):

Hosna Ara Perven ◽

Abu Sadat Mohammad Nurunnabi ◽

Dilruba Siddiqua ◽

Fatema Johora ◽

Halima Afroz ◽

...

Keyword(s):

Histological Study ◽

Age Groups ◽

Age Group ◽

Cross Sectional ◽

Medical College ◽

Group A ◽

The Mean ◽

The Difference ◽

Group B ◽

The Right

This cross sectional, descriptive study was done in the Department of Anatomy, Dhaka Medical College, Dhaka from January to December 2009, to determine the proportion of cortex and medulla of the ovary in di_erent age group of Bangladeshi women. This study was based on collection of 140 ovaries of 70 unclaimed female dead bodies from the morgue of Dhaka Medical College, Dhaka. The samples were divided into three age-groups including A (10-13 years), B (14-45 years) & C (46-52 years). Histological slides were prepared by using routine haematoxylin and eosin stain. Ten best prepared histological slides from each age group were examined to determine the thickness of the cortex and medulla & proportion of the thickness of the cortex and the medulla of the ovary were expressed in percentages. The mean proportion of the cortex and the medulla of the right ovary were found 80.83±0.58% and 19.17±0.58% in group A, 86.95±1.14% and 13.05±1.14% in group B, 70.53±1.53% and 29.47±1.53% in group C respectively. The mean proportion of the cortex and the medulla of the left ovary were found 80.63±0.58% and 19.37±0.58% in group A, 86.78±1.14% and 13.22±1.14% in group B, 70.41±1.50% and 29.59±1.50% in group C respectively. The difference in mean proportion of the cortex and the medulla was not signi_cant in between the ovaries. However, the difference in mean proportion of the cortex and the medulla of the ovary between group A & group B, group A & group C and group B & group C were statistically significant.Bangladesh Med J. 2015 Jan; 44 (1): 8-10

Download Full-text

130 Increased antral follicle population at 60 days of gestation in Holstein cows with low antral follicle count

Reproduction Fertility and Development ◽

10.1071/rdv32n2ab130 ◽

2020 ◽

Vol 32 (2) ◽

pp. 192

Author(s):

R. G. Droher ◽

F. Morotti ◽

A. Guidugli Lindquist ◽

A. Fonseca Zangirolamo ◽

M. Marcondes Seneda

Keyword(s):

Mixed Model ◽

Linear Mixed Model ◽

Antral Follicle ◽

Generalized Linear Mixed Model ◽

Antral Follicle Count ◽

Antral Follicles ◽

Intermediate Group ◽

The Mean ◽

Main Factors ◽

High Repeatability

The antral follicle count (AFC) has been pointed out as one of the main factors that influence the efficiency of reproductive biotechniques. Thus, AFC is considered a fertility marker, which has high repeatability in the same animal. The objective of this study was to evaluate AFC in cows at AI (nonpregnant) and at two moments of gestation (30 and 60 days). For this, 35 pregnant cows, aged 23 to 99 months, confined in a freestall system, kept in the same environmental conditions (24°46'28”S, 49°56'42”W) and under the same feeding conditions, were selected for this study. The same technician performed the AI of the females, after detection of natural or induced oestrus, with 25mg of Dinoprost (Lutalyse) intramuscularly (IM). We established AFC (antral follicles ≥3mm diameter) using a convex intravaginal transducer at the time of AI and at 30 and 60 days of pregnancy. Females were distributed in groups of low (≤18 follicles, n=11), intermediate (≥24 and ≤28 follicles, n=11), or high AFC (≥30 follicles, n=13). Data were analysed using a generalized linear mixed model (Minitab version 18.1), with significance declared at P ≤ 0.05. In the low AFC group, there was an increase in the mean number of antral follicles in relation to gestation, presenting 14.82±1.36 at the time of AI and 23.45±2.31 and 35.18±3.17 at 30 and 60 days of pregnancy, respectively (P<0.0001). Similarly, the intermediate group presented an increase in mean AFC, from 24.64±0.76 at the time of AI and 29.27±3.32 at 30 days to 43.36±5.91 at 60 days of gestation (P=0.001). There was no difference in the high AFC group at different moments, as these presented 41.92±3.50 at the time of AI, 38.31±3.96 at 30 days, and 50.92±5.49 at 60 days of pregnancy. Although mean AFCs differed (P<0.0001) between the low, intermediate, and high AFC groups at time of AI and at 30 days of gestation, there were no differences in AFC between the three groups at 60 days of gestation (P=0.329). In conclusion, low AFC females presented an increase in the number of antral follicles at gestation moments 30 and 60 days from AI. This finding reinforces the strategy of using this category more efficiently for ovum pickup at the beginning of gestation.

Download Full-text

Morphological changes in the ampula of the uterine tubes in different periods of life of giving women

Medical Science And Education Of Ural ◽

10.36361/1814-8999-2021-22-3-9-12 ◽

2021 ◽

Vol 22 (3) ◽

pp. 9-12

Author(s):

I. A. Balandina ◽

◽

A. M. Nekrasova ◽

Keyword(s):

Fallopian Tube ◽

Morphological Study ◽

Transverse Section ◽

Morphological Changes ◽

Age Groups ◽

Fallopian Tubes ◽

Autopsy Material ◽

Mean Values ◽

The Mean ◽

The Right

The aim of the study is to determine the dynamics of morphological changes in the ampullae of the fallopian tubes at different age periods of life using autopsy material from women giving birth. Materials and research methods. The analysis of the results of a complex morphological study of the ampullae of the fallopian tubes of 150 corpses of giving birth women of adolescence, the first and second periods of adulthood, elderly and old age was carried out. Results. The histological picture revealed regular atrophic changes in all layers of the wall of the ampulla of the fallopian tube. When comparing the mean values in five age groups according to the Kruskell-Wallis test, statistically significant differences in the length of the epithelial lining in the transverse section of the ampulla of the right and left fallopian tubes (p < 0,001), as well as the wall thickness of the ampulla of the right and left fallopian tubes (p < 0,001) have been identified. Conclusion. The results of the study supplement the literature data on morphological changes in the fallopian tube and can be used as a standard reference.

Download Full-text

Study on weight of the postmortem parathyroid glands in a Bangladeshi population

Journal of Dhaka Medical College ◽

10.3329/jdmc.v22i1.15603 ◽

2013 ◽

Vol 22 (1) ◽

pp. 34-38

Author(s):

Sabiha Mahbub ◽

Abu Sadat Mohammad Nurunnabi ◽

Abdul Alim ◽

Rukshana Ahmed ◽

Shamim Ara

Keyword(s):

Age Groups ◽

Parathyroid Glands ◽

Cross Sectional ◽

Medical College ◽

Group A ◽

The Mean ◽

The Difference ◽

Bangladeshi Population ◽

Group B ◽

The Right

Context: A cross-sectional descriptive type of study was designed to find out the difference in weight of the parathyroid glands of Bangladeshi people in relation to age and sex and to compare with the previous studies. Methods: This study was done in the Department of Anatomy, Dhaka Medical College, Dhaka, from January to December 2008. The study was performed on 207 post mortem human parathyroid glands collected from 60 unclaimed dead bodies which were in the morgue under examination in the Department of Forensic Medicine, Dhaka Medical College, Dhaka. The samples were divided into three age-groups including group A (10-30 years), group B (31-60 years) & group C (61-90 years) and the weight of the parathyroids were measured and recorded. Results: The mean weight of the right superior parathyroid glands were 13.43±13.77 mg in group A, 13.86±19.15 mg in group B and 9.25±5.12 mg in group C in males and 16.00±6.69 mg in group A and 8.57±5.97 mg in group B in females respectively. The mean weight of the right inferior parathyroids were 17.78±13.94 mg, 16.75±15.67 mg and 23.00±11.53 mg in group A, B and C respectively in males and 26.00±4.18 mg in group A and 15.20±4.32 mg in group B in females. The mean weight of the left superior parathyroid glands were 13.63±6.08 mg in group A, 10.58±7.57 mg in group B and 11.00±3.74 mg in group C in males and 16.20±7.89 mg in group A and 8.29±5.47 mg in group B in females respectively. The mean weight of the left inferior parathyroid gland was 26.11±24.29 mg, 15.81±10.28 mg and 25.25±25.28 mg in group A, B and C respectively in males and 32.25±13.82 mg in group A and 13.29±5.41 mg in group B in females. No difference was found in the weight of superior parathyroid glands at right side between sexes and age groups excepting in superior and inferior parathyroid glands at left side between group A & B (p<0.05). DOI: http://dx.doi.org/10.3329/jdmc.v22i1.15603 J Dhaka Medical College, Vol. 22, No. 1, April, 2013, Page 34-38

Download Full-text

Age Related Change of Inner Diameter of Ampulla of Fallopian Tube in Bangladeshi Female Cadaver

Journal of National Institute of Neurosciences Bangladesh ◽

10.3329/jninb.v4i2.38930 ◽

2018 ◽

Vol 4 (2) ◽

pp. 137-140

Author(s):

Hasna Hena ◽

Shamim Ara ◽

Rubina Qasim ◽

Dilruba Siddiqua ◽

Fatema Johora ◽

...

Keyword(s):

Fallopian Tube ◽

Age Groups ◽

Uterine Cavity ◽

Fallopian Tubes ◽

Cross Sectional ◽

Age Related ◽

Significant Difference ◽

Inner Diameter ◽

The Mean ◽

The Right

Background: The fallopian tubes act as conduit for spermatozoa to reach the oocyte and to convey the fertilized (egg) to enter the uterine cavity following fertilization. Problems with the fallopian tubes can lead to infertility. Detailed morphological and histological knowledge is essential for the diagnosis and management of fallopian tube disease.Objective: The purpose of the present study was to identify the inner diameter of ampulla of the fallopian tube and its changes with advancing age.Methodology: This descriptive cross-sectional study was conducted in the Department of Anatomy at Dhaka Medical College, Dhaka, Bangladesh from July 2008 to June 2009 for a period of one (01) year. This present study was performed on post mortem fallopian tubes of Bangladeshi female. Among them lowest age was 12 years and highest age was 50 years. Samples were divided into three differential age groups named asgroup A (10 to 13 years), group B (14 to 45 years), and group C (46 to 50 years). All samples were studied morphologically and histologically.Results: The mean inner diameter of the ampulla of the right and left fallopian tubes ranged from 1.99±0.08 to 3.24±0.27 mm. The difference between all the groups were statistically significant (p < 0.001).Conclusion: The mean difference of the inner diameter of the ampulla of the right and left fallopian tubesbetween groupA and groupB and groupB and groupC were statistically significant; however, there was no significant difference between right and left fallopian tube.Journal of National Institute of Neurosciences Bangladesh, 2018;4(2): 137-140

Download Full-text

A Study to Evaluate and Compare Right and Left Condylar Discrepancy between Centric Relation and Maximum Intercuspation in Three Age Groups

International Journal of Medical and Dental Sciences ◽

10.19056/ijmdsjssmes/2016/v5i1/83540 ◽

2016 ◽

Vol 5 (1) ◽

pp. 984

Author(s):

Chetan Hegde ◽

Swapnil Mahade ◽

Krishna Prasad D.

Keyword(s):

Age Groups ◽

Orientation Relation ◽

Group Iii ◽

Area Of Interest ◽

Centric Relation ◽

Group I ◽

Left And Right ◽

The Mean ◽

Group Ii ◽

The Right

Background:Since discrepancy between centric relation and maximum intercuspation has been an area of interest for dental fraternity for decades, the study was conducted to expand the concepts of the same when age is taken into consideration.Objective: This study was conducted to assess and measure the discrepancy between centric relation (CR) and maximum intercuspation (MI) at right and left condyles in three age groups, compare the discrepancy at right and left condyles between three age groups and right and left side condyles within the same age group.Methods: Sixty healthy subjects were selected and divided into three groups of twenty subjects each. Group I: 18-25 years, Group II: 30-45 years and Group III: more than fifty years. Preliminary impressions were made. Orientation relation was transferred to a semi-adjustable arcon articulator. Subjects were guided into centric relation using Dawson's bimanual manipulation technique and centric interocclusal record was made. The mandibular cast was related to the maxillary cast using centric interocclusal record. The casts were then allowed to fall into maximum intercuspation and the distance that the condylar analogues had moved was measured using Feeler gauge.Results: The mean CR-MI discrepancy in Group I was 0.417±0.137 mm and 0.364±0.123 mm, Group II was 0.528±0.160 mm and 0.512±0.158 mm and Group III was 0.873±0.228 mm and 0.815±0.172 mm at the right and left condyles respectively.Conclusion: Within the limitations of the study it was concluded that all the sixty subjects had a CR-MI discrepancy in both left and right condyles. The variation in discrepancy between the three age groups was found to be very highly significant.

Download Full-text