scholarly journals Co-inoculation of rhizobia, azospirilla and cyanobacteria for increasing common bean production

2020 ◽  
pp. 2015-2028
Author(s):  
Eduardo Hélder Horácio ◽  
Claudemir Zucareli ◽  
Freddy Zambrano Gavilanes ◽  
João Sarkis Yunes ◽  
Alisson Wilson dos Santos Sanzovo ◽  
...  

The combined inoculation of Rhizobium (R. tropici+R. freirei), Azospirillum brasilense, and Anabaena cylindrica, a diazotrophic cyanobacterium, is a technology that has not yet been tested and established in the production of the common bean (Phaseolus vulgaris). The inoculation may be a promising strategy for increasing crop productivity by combining the benefits of biological nitrogen fixation with the production of plant growth phytohormones. Therefore, the objective of this study was to evaluate the co-inoculation of Rhizobium, Azospirillum brasilense, and Anabaena cylindrica as an alternative method for optimizing the symbiotic performance and development of the common bean at greenhouse conditions. The treatments were as follows: (T1) control without N and inoculation, (T2) N addition (100 kg N ha-1), (T3) Riz (addition of R. tropici+R. freirei), (T4) Azo (Azospirillum brasilense addition), (T5) Ana (Anabaena cylindrical addition), (T6) Riz+Azo, (T7) Riz+Ana, (T8) Azo+Ana, (T9) Riz+Azo+Ana. We used a completely randomized experimental design with four replications. The co-inoculation of Riz+Azo+Ana promoted plant height, root length and volume, shoot dry matter, accumulated shoot N, number and dry matter of nodules at flowering, number of grains per pod, hundred seed weight, and grain production of the common bean, contributing to increased yield per plant. We observed an increase in common bean grain yield ranging from 62 to 84% after double and triple co-inoculation of rhizobia with azospirilla and/or cyanobacteria, with the highest yield observed in the plants inoculated with Riz+Azo+Ana (84%), similar to those observed in plants after N addition. However, field experiments are necessary to elucidate the performances of the inoculated beneficial microorganisms.

Author(s):  
Alinne da Silva ◽  
◽  
Vinícius I. Franzini ◽  
Cristiano D. Piccolla ◽  
Takashi Muraoka ◽  
...  

ABSTRACT The common bean has been considered to have low biological nitrogen fixation capacity; however, this process can be made more effective with molybdenum (Mo) supplementation. The objective of this study was to evaluate the influence of Mo rates on the growth and biological nitrogen fixation by two Brazilian common bean cultivars using the 15N isotope dilution technique. The experiment was performed in 2014 in a completely randomized design arranged in a 5 x 3 factorial scheme, corresponding to 5 rates of Mo (control, 40, 80, 120 and 240 g ha-1), the common bean cultivars Aporé, Ouro Negro and NORH-54 (a non-nodulating common bean cultivar), and three replicates. The application of Mo and the inoculation with rhizobia strains contributed to improving nitrogen fixation and grain weight. The cultivar Ouro Negro showed a higher number and weight of nodules and a higher amount of nitrogen derived from the atmosphere than the cultivar Aporé. The biological nitrogen fixation of Aporé was more dependent on the application of Mo. These results indicated that inoculation with Rhizobium strains and Mo supply effectively contributed to biological nitrogen fixation and improving grain production.


2017 ◽  
Vol 35 (0) ◽  
Author(s):  
L.F. CIESLIK ◽  
R.A. VIDAL ◽  
A.B. MACHADO ◽  
M.M. TREZZI

ABSTRACT Grass weeds are common in summer crops and strongly decreases the grain yield of the common bean crop. The time of herbicide application influences the variability of environmental conditions and affects the product performance. The objectives of this work were to identify the time of fluazifop-p-butyl (fluazifop) application which gives best grass weed control in the common bean crop and to elucidate the environmental variables most important for the efficacy of this herbicide. Field experiments were conducted involving five application times (2 a.m., 6 a.m., 11 a.m., 4 p.m. and 9 p.m.) and five doses of fluazifop (80, 110, 140, 170 and 200 g ha-1), with additional no-herbicide control. At the time of the herbicide application it was determined the air temperature, relative humidity, the photosynthetically active radiation (PAR) and the leaf angle, whereas the weed control and the dry mass of the weed Urochloa plantaginea was assessed at 20 days after treatment (DAT). Efficacy on grass control with fluazifop was dependent on the herbicide dose and on the time of day that the product was applied. Spray at early morning hours (6 a.m.) showed better efficacy on weed control in relation to periods during warmer conditions of the day (11 a.m. and 4 p.m.). Nocturnal fluazifop application had better weed control when compared to herbicide sprayed in the afternoon. The air temperature, relative humidity and PAR were correlated to weed leaf angle, which correlated the most with fluazifop performance.


2018 ◽  
Vol 36 (0) ◽  
Author(s):  
F.B. PAGNONCELLI JR ◽  
R.A. VIDAL ◽  
M.M. TREZZI ◽  
M. GALLON ◽  
A.P. BRUSAMARELLO

ABSTRACT: In literature, there are few studies evaluating the response of different bean cultivars to herbicides, particularly the ALS enzyme inhibitors. The objective of this study was to evaluate the selectivity of common bean cultivars to the herbicide ethoxysulfuron and to select cultivars that are more tolerant to it. An experiment was conducted in a greenhouse, in a completely randomized experimental design with four replications. Factors consisted in 20 bean cultivars (IPR Eldorado, IPR Siriri, IPR Gralha, IPR 81, IPR Uirapuru, IPR Colibri, IPR Tuiuiú, BRS Esplendor, IPR Tangará, IPR Juriti, IPR Corujinha, IPR Andorinha, IPR Curió, BRS Campeiro, BRS Pérola, BRS Notável, BRS Estilo, UTF 3, UTF 5 e UTF 6), and four doses of ethoxysulfuron (0, 50, 100 and 200 g ha-1) were used. At the dose of 200 g ha-1, the reduction of the shoot dry matter of plants ranged from 30 to 80%, indicating that there was high variability in the response of bean cultivars to the herbicide. The bean cultivars classified as more tolerant to ethoxysulfuron are: IPR 81, IPR Uirapuru, BRS Estilo, IPR Gralha and BRS Pérola, while the less tolerant cultivars are: IPR Eldorado, IPR Tuiuiú, IPR Tangará, IPR Curió, UTF 3, UTF 5 and BRS Esplendor.


2020 ◽  
Vol 41 (6supl2) ◽  
pp. 2967-2976
Author(s):  
José Geraldo da Silva ◽  
◽  
Enderson Petrônio de Brito Ferreira ◽  
Virgínia Damin ◽  
Adriano Stephan Nascente ◽  
...  

The common bean production system is majorly based on the use of granular fertilizers to provide nutrients for the crop. Studies on the use of liquid fertilization at an appropriated depth and, seed inoculation with Rhizobium tropici can provide significant increases in the grain yield of the common bean. The objective of this study was to determine the growth and productivity of common bean as affected by N-P formulations, application depths and the inoculation with R. tropici. Field experiments were carried out in 2015 and 2016 cropping years using a complete block design, in a 2x2x2 factorial arrangement, with four replicates. The treatments involved the combination of N-P formulation (granular and liquid), two application depths of the N-P formulation (6 and 12 cm) and with or without rhizobia inoculant. The plant density (PD), number of pods (NP), number of grains (NG), mass of 100 grains (M100) and grain yield (GY) were determined. The granular and liquid N-P formulations provided similar results for PD, NP, NG, and GY of common bean. Similarly, the application depth of the N-P formulations did not affect GY. Inoculation of the seed with R. tropici stimulated NP and NG, increasing GY. Growth and productivity parameters were equally affected by the type of formulation and application depth; however, GY was greater with rhizobial inoculant. Thus, the liquid N-P formulation, applied at 6 cm depth, associated with rhizobial inoculant can improve the crop management providing better control of application uniformity, minimal soil mobilization, less fuel consumption, and increased grain yield.


Author(s):  
Fabiano J. de C. Bastos ◽  
Frederico A. L. Soares ◽  
Camylla V. Sousa ◽  
Cássio J. Tavares ◽  
Marconi B. Teixeira ◽  
...  

ABSTRACT The objective of this study was to evaluate the performance of the common bean crop grown with application of osmoprotectants based on algae of the genus Durvillaea potatorum in the winter crop season, with occurrence or not of water suppression. The experiment used a randomized block design, in split plots, arranged in a 4x7 factorial scheme, in which the plots were composed of four treatments - periods of water suppression (7, 14, 21 days and the control treatment: 100% of field capacity throughout the crop cycle) and the subplots consisted of seven types of osmoprotectants. The treatments with osmoprotectants was applied during the full flowering of the common bean. Three days after application of osmoprotectants, the treatments with suppression of irrigation were established. The use of osmoprotectants based on algae of the genus Durvillaea does not affect the variables plant height, stem diameter, stem and pod dry matter, first pod height, number of pods per plant and number of grains per pod in common bean plants cultivated with occurrence of water suppression. Common bean plants under water restriction conditions have lower leaf and shoot dry matter and lower 100-grain weight. Common bean grain yield was influenced by the type of osmoprotectants and the water suppression period.


2021 ◽  
pp. 12-17
Author(s):  
Oleksandr Chynchyk ◽  
Svitlana Olifirovych ◽  
Volodymyr Olifirovych ◽  
Kateryna Nebaba

The results of a field experiment to study the effect of seed treatment with the biological product Rhizoactive (сompany BioNorma, Ukraine) on the formation and functioning of the symbiotic apparatus of common bean (grain) plants are presented. The experiment examined six varieties of common beans (grain), included in the State Register of plant varieties suitable for distribution in Ukraine: Bukovynka, Halaktyka, Slaviia, Ros, Otrada, Nata. The influence of bacterial fertilizer based on nodule bacteria " Rhizoactive " on the indicators of symbiotic and grain productivity of these varieties of common beans was studied. It was found, that the maximum number and raw weight of active nodules in the studied varieties of common beans were formed in the flowering phase. A larger number of nodules, compared to the variety Bukovynka (control), was formed on the root system of bean varieties Ros, Otrada and Nata. The maximum raw weight of rhizobia in the plots without the use of Rhizoactive was formed by the common bean variety Otrada – 0.297 g/plant of active nodules. Inoculation of the seeds of the studied common bean varieties with Rhizoactive increased the raw weight of active nodules in the flowering phase by 0.016–0.042 g/plant. A larger mass of nodules, compared to the Bukovynka variety, was formed on the root system of Ros, Otrada and Nata beans. The maximum raw weight of rhizobia in areas without the use of Rhizoactive was formed by the variety of beans Otrada – 0.297 g/plant of active nodules. Inoculation of seeds of the studied varieties of beans with Rhizoactive increased the raw weight of active nodules in the flowering phase by 0.016–0.042 g/plant. The highest increase in grain yield from seed inoculation was obtained in 2018, which was more favorable in terms of moisture. Thus, the largest increase in grain yield from the use of Rhizoactive was provided by the varieties Halaktyka (0.14 t/ha or 5.7 %), Ros (0.15 t/ha or 5.5 %) and Otrada (0.22 t/ha or 7.7 %). On average, in 2018–2020, inoculation of bean seeds with Rhizoactive, depending on the variety, provided an increase in grain yield from 2.4 to 6.7 %. And the highest level of bean grain yield on average in 2018–2020 was provided by the varieties Otrada (2.60 t/ha), Nata (2.50 t/ha) and Ros (2.40 t/ha) for inoculation of seeds with Rhizoactive


2000 ◽  
Vol 57 (1) ◽  
pp. 153-157 ◽  
Author(s):  
Itamar Pereira de Oliveira ◽  
Colin J. Asher ◽  
David G. Edwards ◽  
Renato S. Mota dos Santos

Magnesium applications (MgS0(4).7H(2)0) to achieve 8 and 16 mmol c/cm3 of Mg (324 and 1284 kg of MgS0(4) ha-1) were made on one Ultisol from Australia Northeast to correct Mg deficiency in plants and to verify the optimum level of Mg to grow common bean (Phaseolus vulgaris L). Magnesium was applied together with lime, N, K, Cu, Zn, B, and Mo as calcium carbonate, ammonium nitrate, potassium phosphate, cupric and zinc sulphate, boric acid and sodium molybdate respectively a month before planting and P as phosphoric acid at the planting. The pH reached the equilibrium after six weeks of incubation. Higher electrical conductivity (EC) was observed in soil where Mg was applied to reach 8 mmol c/cm3 in the absence of common bean and 16 mmol c/cm3 when the plant was present. Higher plant height, leaf area, dry matter weight:leaf area ratio and nutrient concentrations were observed in plants cultivated in soils treated with Mg to reach 8 mmol c/cm3 and 16 mmol c/cm3 when the plant was present. The plant top P content was very low but N, Ca and Mg contents can be considered normal for the common bean.


2021 ◽  
Vol 34 (4) ◽  
pp. 857-866
Author(s):  
JOSÉ GERALDO DA SILVA ◽  
ENDERSON PETRÔNIO DE BRITO FERREIRA ◽  
ADRIANO STEPHAN NASCENTE

ABSTRACT The use of filler liming in the sowing furrow can improve the chemical characteristics of the soil and, together with nitrogen fertilization, increase common bean yield. The objective of this study was to determine the effect of filler liming of the sowing furrow along with nitrogen topdressing fertilization on the yield of common bean, cultivar Pérola, irrigated by central pivot, in the Cerrado Region. The field experiments were conducted for three consecutive cropping years, in a randomized block design with four replications, in a 2x4 factorial scheme, consisting of two doses of nitrogen topdressing fertilization (zero and 60 kg ha-1 of N) and four doses of filler liming application in the sowing furrow (0, 200, 400 and 600 kg ha-1 of CaCO3). The plant density (PD), number of pods (NP), number of grains (NG), mass of 100 grains (M100) and, grain yield (GY) were evaluated. The use of 60 kg ha-1 of N provided greater M100 and GY. The increase of the filler liming doses in the sowing furrow led to a reduction of the NP. The filler liming dose of 200 kg ha-1 provided higher values of NG and M100, and when combined with the nitrogen topdressing fertilization, improved the GY of the common bean.


2017 ◽  
Vol 48 (3) ◽  
Author(s):  
Lourdes Dickmann ◽  
Marcelo Andreotti ◽  
Marcelo Fernando Pereira Souza ◽  
Allan Hisashi Nakao ◽  
Gabriela Christal Catalani

Author(s):  
Priscila Flôres Aguirre ◽  
Sandro José Giacomini ◽  
Clair Jorge Olivo ◽  
Vinicius Felipe Bratz ◽  
Maurício Pase Quatrin ◽  
...  

Abstract: The objective of this work was to quantify the inoculation effect of Azospirillum brasilense (Ab-V5 and Ab-V6 strains) on the forage yield, biological nitrogen fixation (BNF), and urea-15N recovery of the forage grass 'Coastcross-1'. The experiment was carried out in a 2 (with or without inoculation) × 2 (without N fertilizer and with 100 kg ha-1 N per year as urea) × 7 (cuts) factorial arrangement. The natural 15N abundance method was used to determine BNF; to determine urea-N recovery, 15N-labeled urea was applied in microplots. Forage yield was higher in grasses subjected to inoculation, with 7.4 Mg ha-1 dry matter per year, for the treatment without N fertilizer, and 8.0 Mg ha-1 dry matter per year for the treatment with 100 kg ha-1 N per year, respectively, which shows an additive effect of inoculation and N fertilization. The contribution of BNF was 23.0 and 53.8 kg ha-1 per year for the unfertilized treatment, both in uninoculated and inoculated plants, respectively. Urea-15N recovery was 13.7 and 16.5 kg ha-1 per year for uninoculated and inoculated treatments, respectively, corresponding to 13.7 and 16.5% of the urea-N applied. Inoculation with A. brasilense increases forage yield and the contribution of BNF to grass nutrition with N, as well as urea-N recovery by the forage grass.


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